Article
Agriculture, Dairy & Animal Science
Jalila S. Dridi, Elizabeth S. Greene, Craig W. Maynard, Giorgio Brugaletta, Alison Ramser, Courtney J. Christopher, Shawn R. Campagna, Hector F. Castro, Sami Dridi
Summary: Heat stress is a major issue in poultry production, affecting growth, welfare, and gut integrity. This study used high-throughput metabolomics to analyze the metabolite profiles in the duodenum of heat-stressed broilers. The results showed that heat stress altered several duodenal metabolites in a duration-dependent manner and identified potential metabolite signatures. The study also identified specific pathways and diseases associated with heat stress. These findings provide valuable insights into the mechanisms underlying heat stress response in poultry and could contribute to the development of strategies to mitigate its impact.
Article
Chemistry, Multidisciplinary
Ke Wang, Xiao-Ping Peng, Dan Feng, Chuan Tian, Wen-Long Wan, Hong-Xiang Lou, Gang Li
Summary: In this study, a variety of GAs compounds were isolated from a fungus and their spectroscopic data were reported. Compound 1 showed a promoting effect on the growth of Arabidopsis thaliana seedlings.
Article
Environmental Sciences
Shiyuan Zhao, Haitao Zhong, Chunmei Geng, Hongjia Xue, Changshui Wang, Wenxue Sun, Ruili Dang, Wenxiu Han, Pei Jiang
Summary: In this study, gas chromatography-mass spectrometry was used to investigate the toxic mechanisms of ACR in the main target tissues of rats, revealing 14 metabolic pathways related to amino acid, fatty acid, purine, and energy metabolism. The study suggests that the toxic mechanism of ACR may involve oxidative stress, inflammation, amino acid metabolism, and energy disorders.
ENVIRONMENTAL POLLUTION
(2021)
Article
Neurosciences
Carolina Gonzalez-Riano, Silvia Tapia-Gonzalez, Gertrudis Perea, Candela Gonzalez-Arias, Javier DeFelipe, Coral Barbas
Summary: Brain slice preparations undergo significant changes in metabolome and lipidome, including adjustments in levels of various lipid classes and metabolites. These preparation-dependent biochemical changes related to time intervals should be considered in future studies to facilitate unbiased interpretation of experimental results.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Chemistry, Analytical
Huihui Liu, Ting Xie, Jiyun Wang, Xiao Wang, Jing Han, Zhihua Huang, Lixia Jiang, Zongxiu Nie
Summary: HIE is a major cause of neurological disability and fetal or neonatal asphyxia death. The study found significant changes in metabolites involved in multiple metabolic pathways during the HIE period, including the accumulation of neutral TGs in the ischemic region.
Article
Chemistry, Multidisciplinary
Yifan Meng, Elumalai Gnanamani, Richard N. Zare
Summary: Water microdroplets can directly produce stable C7H7+ cations from toluene and other substrates, and the reaction is driven by hydroxyl radicals generated at the water-gas interface of the microdroplets. Primary, secondary, and tertiary amines in the microdroplets can react with C7H7+ and benzyl radicals to form alkyl C(sp3)-N coupling products in one step.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Applied
Zhibin Yin, Tao Dong, Wenjie Huang, Mingyi Du, Dong Chen, Alisdair R. Fernie, Ganjun Yi, Shijuan Yan
Summary: This study investigated the spatiotemporal distribution and levels of metabolites within Brazil and Dongguan banana pulps during postharvest senescence. The results showed that both postripening stages and banana varieties influenced metabolite levels. The use of gold nanoparticle-assisted laser desorption/ionization mass spectrometry imaging (LDI-MSI) allowed for the visualization of sugars, amino acids, and monoamines within the pulps for the first time.
Article
Pharmacology & Pharmacy
Shereen M. Aleidi, Lina A. Dahabiyeh, Xinyun Gu, Mohammed Al Dubayee, Awad Alshahrani, Hicham Benabdelkamel, Muhammad Mujammami, Liang Li, Ahmad Aljada, Anas M. Abdel Rahman
Summary: Metformin has metabolic effects in T2DM and obesity, with 30 dysregulated metabolites in obese diabetic patients restored to control levels by metformin. Interestingly, 9 metabolites were significantly dysregulated between lean and obese cohorts due to T2DM and metformin regardless of obesity status, providing insight into potential molecular targets of metformin in T2DM and obesity.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Yuming Jiang, Alexandre Hutton, Caleb W. Cranney, Jesse G. Meyer
Summary: This article introduces an improved direct infusion and ion mobility method for proteome analysis, and proposes a label-free quantification method DISPA-LFQ, which can rapidly identify and quantify proteins in complex mixtures. This technology has important applications in drug screening, biomarker discovery, and clinical analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Pierluigi Reveglia, Carmela Paolillo, Antonella Angiolillo, Gabriella Ferretti, Ruggero Angelico, Rossana Sirabella, Gaetano Corso, Carmela Matrone, Alfonso Di Costanzo
Summary: Alzheimer's disease is the most common cause of dementia in the elderly population. It not only affects the brain, but also disturbs the overall metabolism of the body. Analysis of blood samples from patients with AD revealed significant dysregulation of metabolites, including upregulation of glycerophospholipids and ceramide, and downregulation of glutamic acid, other phospholipids, and sphingolipids. Pathway analysis showed that at least five metabolic pathways related to polar compounds were dysregulated in AD patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Yanan Li, Dadi Xie, Luxi Li, Pei Jiang
Summary: This study aimed to explore the metabolic changes induced by hypertension at different ages. The results showed that spontaneously hypertensive rats (SHR) at 5 weeks old and 32 weeks old had different metabolic characteristics, mainly involving amino acid metabolism, glycolysis, lipid metabolism, and amino acid metabolism.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2023)
Article
Surgery
Richmond T. Bergner, Sarah Onida, Rahul Velineni, Konstantina Spagou, Manjit S. Gohel, Marielle Bouschbacher, Serge Bohbot, Joseph Shalhoub, Elaine Holmes, Alun H. Davies
Summary: The study aimed to identify potential biomarkers predictive of healing or failure to heal in venous leg ulceration. It found that serum biofluids could differentiate between healing and nonhealing patients, and the ratio of carnitine to ceramide could differentiate between healing phenotypes with 100% sensitivity, 79% specificity, and 91% accuracy. This metabolic signature has potential applications for disease prognostication and development of targeted therapies for venous leg ulceration.
Article
Medicine, General & Internal
Yun Hwan Oh, Seulggie Choi, Gyeongsil Lee, Joung Sik Son, Kyae Hyung Kim, Sang Min Park
Summary: The study found that increasing relative lean body mass and appendicular skeletal mass can reduce the risk of metabolic syndrome in both men and women, while increasing relative body fat mass index can increase the risk of metabolic syndrome. Particularly, the risk reduction of MetS is more significant in groups with higher baseline BMI and body fat mass index.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Shreyas Deshpande, Sirsha Mitra
Summary: Beta-cyclocitral (beta CC) is an important oxidative product of beta-carotene that enables plants to acclimate to multiple stresses while maintaining growth. This study demonstrates the metabolic changes induced by beta CC that promote defense and growth in tomato plants under stress. The findings show that beta CC specifically affects amino acid and phenolic metabolism in tomato plants.
Article
Biochemistry & Molecular Biology
Linqiang Gong, Shiyuan Zhao, Xue Chu, Hui Yang, Yanan Li, Shanshan Wei, Fengfeng Li, Yazhou Zhang, Shuhui Li, Pei Jiang
Summary: This study investigated the metabolic changes in multiple tissues induced by cold exposure in mice. The results showed that cold exposure effectively modulated adipose tissue metabolism and improved metabolic health. Amino acids, fatty acids, and energy metabolism were found to be the most significantly altered compounds, and these changes may provide new insights for treating metabolic diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Shih-Chao Lin, Brian D. Carey, Victoria Callahan, Ji-Hyun Lee, Nicole Bracci, Anurag Patnaik, Amy K. Smith, Aarthi Narayanan, Benjamin Lepene, Kylene Kehn-Hall
Article
Chemistry, Multidisciplinary
Elizabeth E. Pierson, Anthony J. Midey, William P. Forrest, Vivek Shah, Hernando J. Olivos, Bindesh Shrestha, Ryan Teller, Seth Forster, Alena Bensussan, Roy Helmy
PHARMACEUTICAL RESEARCH
(2020)
Article
Thermodynamics
J. Houston Miller, Rachelle J. Golden, Jennifer A. Giaccai, Andrew Kamischke, Andrew Korte, Akos Vertes
Summary: Our laboratory demonstrated a method to precisely determine and spatially resolve the PAH composition of soot using low-fluence laser desorption ionization coupled with high-resolution mass spectrometry imaging. The majority of observed PAH species were found to have benzenoid structures with highly condensed configurations.
PROCEEDINGS OF THE COMBUSTION INSTITUTE
(2021)
Article
Chemistry, Multidisciplinary
Laith Z. Samarah, Tina H. Tran, Gary Stacey, Akos Vertes
Summary: This study demonstrated the complementary analysis and MSI of three biological polymers in soybean root nodule sections using NAPA-LDI and MALDI. NAPA-LDI-MSI showed improved performance in characterizing the molecular distributions in tissue sections and regions of interest.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Virology
Bibha Dahal, Caitlin W. Lehman, Ivan Akhrymuk, Nicole R. Bracci, Lauren Panny, Michael D. Barrera, Nishank Bhalla, Jonathan L. Jacobs, Jonathan D. Dinman, Kylene Kehn-Hall
Summary: The research revealed that the loss of PERK significantly reduced VEEV and EEEV infectious titers in human primary astrocytes, pericytes, and endothelial cells. Moreover, PERK depletion also affected nonstructural protein translation and RNA production of various RNA viruses, suggesting that PERK is a potential target for antiviral drug development.
Review
Neurosciences
Ellen A. Wood, Sylwia A. Stopka, Linwen Zhang, Sara Mattson, Gabor Maasz, Zsolt Pirger, Akos Vertes
Summary: The great pond snail Lymnaea stagnalis is a well-established model organism with a simple central nervous system that has been extensively used to study neural functioning. Through electrophysiological experiments and mass spectrometry techniques, researchers have mapped neuronal connections and identified circuits responsible for basic life functions. This combination of techniques has led to increasing insights into the molecular basis of CNS-controlled biological functions.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Yuriy Kim, Gifty A. Mensah, Sarah Al Sharif, Daniel O. Pinto, Heather Branscome, Sowmya V. Yelamanchili, Maria Cowen, James Erickson, Pooja Khatkar, Renaud Mahieux, Fatah Kashanchi
Summary: The study found differential secretion of EV and autophagy-related proteins in HIV-1 and HTLV-1 infected cells, with viral protein and RNA production correlating with viral release. Furthermore, EV release may occur prior to viral release from infected cells, potentially impacting uninfected recipient cells before subsequent viral infection and spread.
Article
Biochemistry & Molecular Biology
Michael J. Taylor, Sara Mattson, Andrey Liyu, Sylwia A. Stopka, Yehia M. Ibrahim, Akos Vertes, Christopher R. Anderton
Summary: Single cell analysis, particularly using LAESI-MS technology, allows for advantages in metabolomics; integrating a microscope with the LAESI source enables visually informed ambient in situ single cell analysis; coupling with a drift-tube ion mobility mass spectrometer can enhance confidence in structural identification of molecules from single cells.
Article
Chemistry, Analytical
Sylwia A. Stopka, Ellen A. Wood, Rikkita Khattar, Beverly J. Agtuca, Walid M. Abdelmoula, Nathalie Y. R. Agar, Gary Stacey, Akos Vertes
Summary: This study utilized mass spectrometry imaging (MSI) to analyze tissue-embedded single cells in high throughput, achieving accurate single-cell sampling through automated image analysis and discovering cellular phenotypes characterized by differing metabolite levels.
ANALYTICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Shih-Chao Lin, Caitlin W. Lehman, Allison K. Stewart, Lauren Panny, Nicole Bracci, Jeffrey L. C. Wright, Mikell Paige, Wendy K. Strangman, Kylene Kehn-Hall
Summary: The study isolated a potential anti-alphavirus compound, Homoseongomycin, from marine microbes, which showed significant antiviral activity against Venezuelan and eastern equine encephalitis viruses with low cytotoxicity.
ANTIVIRAL RESEARCH
(2021)
Article
Biochemical Research Methods
Michael J. Taylor, Andrey Liyu, Akos Vertes, Christopher R. Anderton
Summary: LAESI-MS is an emerging method with the potential to revolutionize metabolomics, offering the advantages of minimal sample preparation and in situ analysis with high spatial resolution for cells and tissues.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Virology
Nicole Bracci, Cynthia de la Fuente, Sahar Saleem, Chelsea Pinkham, Aarthi Narayanan, Adolfo Garcia-Sastre, Velmurugan Balaraman, Juergen A. Richt, William Wilson, Kylene Kehn-Hall
Summary: RVFV, a virus causing significant disease in humans and livestock, was first reported in the Rift Valley of Kenya. In this study, host proteins interacting with the Gn protein of RVFV were identified using a proteomics approach. Depletion of UBR4, a host protein, resulted in a decrease in RVFV titers and viral RNA production.
Article
Pharmacology & Pharmacy
Lauren Panny, Ivan Akrhymuk, Nicole Bracci, Caitlin Woodson, Rafaela Flor, Isaac Elliott, Weidong Zhou, Aarthi Narayanan, Catherine Campbell, Kylene Kehn-Hall
Summary: Venezuelan equine encephalitis virus (VEEV) is transmitted by mosquitos and can cause febrile illness and severe neurological complications. There are currently no FDA approved vaccines or antiviral treatments available. A study identified protein disulfide isomerase family A member 6 (PDIA6) as a critical regulator of alphavirus replication. Inhibition of PDIs through LOC14 or nitazoxanide treatment effectively reduced production of VEEV and other alphaviruses in vitro.
ANTIVIRAL RESEARCH
(2023)
Article
Chemistry, Analytical
Marjan Dolatmoradi, Sylwia A. Stopka, Chloe Corning, Gary Stacey, Akos Vertes
Summary: In this study, a mass spectrometry enhanced with ion mobility separation and coupled with an automated sampling platform was utilized for in situ high-throughput single-cell metabolomics in soybean root nodules. The system achieved a high sampling rate and improved molecular coverage, leading to the discovery of metabolic heterogeneity. The upgraded platform allowed for the characterization of both unimodal and bimodal metabolite abundance distributions, revealing the presence of distinct subpopulations.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Marjan Dolatmoradi, Laith Z. Samarah, Akos Vertes
Summary: Cellular heterogeneity is an important property of cell populations that can be studied through single-cell metabolomics, which reveals molecular information and variations in metabolite concentrations. Mass spectrometry techniques face challenges in analyzing single cells, but can potentially uncover cellular synergism through tissue-embedded cell analysis.
ANALYSIS & SENSING
(2022)