Article
Plant Sciences
Rajtilak Majumdar, Paul J. Galewski, Imad Eujayl, Rakesh Minocha, Eric Vincill, Carl A. Strausbaugh
Summary: Beet curly top virus (BCTV) is a significant threat to sugar beet production worldwide. This study focuses on the role of sugar beet microRNAs in the resistance to BCTV during early infection stages. The findings provide insights into the molecular mechanisms of resistance and the regulation of metabolic pathways in sugar beet.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Atiyeh Bahari, Araceli G. Castillo, Naser Safaie, Eduardo R. Bejarano, Ana P. Luna, Masoud Shams-Bakhsh
Summary: In this study, it was found that the V2 gene of BCTIV, one of the main causal agents of beet curly top disease in Iran, has similar function to curtoviruses in suppressing local silencing and delaying systemic silencing spreading. The V2 gene of BCTIV also has the same subcellular localization as curtoviruses in the nucleus and perinuclear region. Additionally, overexpression of BCTIV V2 gene can induce necrotic lesions and systemic infection in Nicotiana benthamiana.
Article
Biochemistry & Molecular Biology
Rajtilak Majumdar, Carl A. Strausbaugh, Eric D. Vincill, Imad Eujayl, Paul J. Galewski
Summary: Through sequencing and analysis of leaf bacteriome changes in different sugar beet genotypes in response to Beet curly top virus, this study identified potential bacteria with antiviral properties, providing insights for their use as biocontrol agents or biomarkers against the virus in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
M. Saadati, A. Rajabi, M. Shams-Bakhsh
Summary: The study aimed to identify sugar beet genotypes resistant to Severe strain of Beet Curly Top Virus and Beet Curly Top Iran Virus. It was found that ten genotypes were resistant to BCTV-Svr and seven genotypes were resistant to BCTIV, with six genotypes identified as resistant to both viruses.
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
(2021)
Article
Entomology
Rebecca Creamer, Annabel Simpson, Hanah T. Rheay, Catherine E. Brewer
Summary: The beet leafhopper, the sole carrier of beet curly top virus, has been found to infect industrial hemp in recent years. This study evaluated the interaction between the beetle leafhopper and hemp in New Mexico by examining the natural infection rate and monitoring leafhopper numbers using sticky traps. Higher numbers of leafhoppers were observed in hemp fields in 2022 compared to 2021, and the beet curly top virus infected multiple types and varieties of hemp.
ENVIRONMENTAL ENTOMOLOGY
(2023)
Article
Plant Sciences
Tomas A. Melgarejo, Li-Fang Chen, Maria R. Rojas, Annemiek Schilder, Robert L. Gilbertson
Summary: Commercial fields of industrial hemp in California showed symptoms similar to curly top disease caused by beet curly top virus. The virus infection was detected in 89% of hemp samples with curly top symptoms and primarily consisted of mild-type strains. This study confirms the potential economic impact of the virus on processing tomato production.
Article
Biochemical Research Methods
Kubilay Yildirim, Musa Kavas, Riza Kaya, Zafer Secgin, Cansu Can, Ilkay Sevgen, Cigdem Gokcek Sarac, Vahid Tahan
Summary: In this study, the genome based characterization of BCTD-associated viruses in Turkish sugar beet fields was conducted, confirming that the virus belongs to the beet curly top Iran virus species. The spread of this virus poses a significant threat to sugar beet and other agricultural crops.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Biochemistry & Molecular Biology
Kubilay Yildirim, Musa Kavas, Ilkay Sevgen Kucuk, Zafer Secgin, Cigdem Gokcek Sarac
Summary: This study demonstrated the efficiency of gRNA/Cas9 constructs in improving sugar beet resistance against BCTIV. The CRISPR-mediated broad-spectrum resistance showed great potential in protecting sugar beet from viral infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Omid Eini, Nadine Schumann, Markus Niessen, Mark Varrelmann
Summary: Researchers have developed a geminiviral replicon from BCTV with a wide host range and high DNA accumulation capacity to efficiently deliver CRISPR/Cas12a components into plant cells. This replicon enabled a higher rate of mutagenesis and HDR in plants, providing a new tool for gene editing in dicotyledonous plants and facilitating further optimization and understanding of HDR in plants using CRISPR/Cas12a system.
Article
Virology
Saeideh Ebrahimi, Omid Eini, Alexandra Bassler, Arvid Hanke, Zeynep Yildirim, Michael Wassenegger, Gabi Krczal, Veli Vural Uslu
Summary: Beet curly top Iran virus (BCTIV) has a unique genome organization and its V2 and Rep genes can suppress different types of gene silencing. Rep can suppress systemic silencing while V2 cannot, as demonstrated by experiments.
Article
Plant Sciences
Alvaro Soler-Garzon, Deidrah Goldoff, Alyson Thornton, Kylie D. Swisher D. Grimm, John P. Hart, Qijian Song, Carl A. Strausbaugh, Phillip N. Miklas
Summary: Dr. Peng Wei and his team used a genome-wide association study to identify a missense single nucleotide polymorphism (SNP) in the candidate gene Phvul.007G036300 Exonuclease V (EXO5) as the most likely causal mutation for beet curly top virus (BCTV) in common bean. The use of three SNP-haplotype assay markers will help researchers track resistance sources and develop bean cultivars with better BCTV resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Mehdi Saadati, Mahdi Ayyari, Masoud Shams-Bakhsh
Summary: This study investigated the effect of beet curly top virus infection on the physical and chemical properties of coriander leaves. The infected plants showed reductions in height, fresh and dry mass, and had impaired flowering and seed production. There was a decrease in the levels of flavonoids, phenolic compounds, and vitamin C in the infected leaves. The essential oil content did not show significant differences between infected and uninfected plants, but the composition of the essential oils changed.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Plant Sciences
Ying Zhai, Anirban Roy, Hao Peng, Daniel L. Mullendore, Gurpreet Kaur, Bikash Mandal, Sunil Kumar Mukherjee, Hanu R. Pappu
Summary: This study identifies four silencing suppressors encoded by Croton yellow vein mosaic virus (CYVMV) and its cognate betasatellite, and investigates their subcellular localizations, interaction behavior, and roles in symptom induction and intercellular virus movement.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sergey Y. Y. Morozov, Alexander A. A. Lezzhov, Andrey G. G. Solovyev
Summary: This paper investigates the genome organization and protein properties of Reclovirids, a potential group within the Benyviridae-related viruses. It suggests that the Reclovirid genomes may contain previously unknown transport genes. Moreover, the coding potential of these novel viral genomes reveals the presence of proteins with hydrophobic segments that are structurally diverse among Reclovirids and unique to other plant RNA viruses. The study also indicates an association of most Reclovirid proteins with cell membranes, which is not observed in annotated members of the Benyviridae family.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Saeideh Ebrahimi, Omid Eini, Davoud Koolivand, Mark Varrelmann
Summary: This study characterizes the complementary-sense transcripts of Beet curly top Iran virus (BCTIV) and identifies Rep and C1 as the main pathogenicity factors that also trigger the hypersensitive response (HR) in plants.
Article
Biochemistry & Molecular Biology
Dan Zhang, Sanyuan Tang, Peng Xie, Dekai Yang, Yaorong Wu, Shujing Cheng, Kai Du, Peiyong Xin, Jinfang Chu, Feifei Yu, Qi Xie
Summary: Researchers successfully developed aromatic sorghum lines with the help of CRISPR/Cas9-mediated knockout of SbBADH2 gene. The fragrant sorghum seeds and leaves are attractive, making them valuable in the sorghum market for both grain production and biomass forage.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Qian Chen, Yaorong Wu, Feifei Yu, Qi Xie
Summary: Endoplasmic reticulum-associated degradation (ERAD) and selective autophagy are two important degradation pathways in plant cells, which cooperate to clear protein aggregates and relieve cellular stress. This review analyzes ERAD and different types of autophagy in plants, and summarizes characterized selective autophagy receptors. Additionally, ERAD components also serve as cargos in selective autophagy.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chengxuan Chen, Xiaoling Shang, Meiyu Sun, Sanyuan Tang, Aimal Khan, Dan Zhang, Hongdong Yan, Yanxi Jiang, Feifei Yu, Yaorong Wu, Qi Xie
Summary: Through comparative transcriptome analysis, it was found that the salt tolerance mechanism in sweet sorghum might be closely related to the early response in secondary metabolism and hormone signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuting Xie, Yeting Gu, Guangping Shi, Jianliang He, Wenjing Hu, Zhonghui Zhang
Summary: This study identified 20 AtPUSs and 22 ZmPUSs from Arabidopsis and maize, respectively. Phylogenetic analysis categorized them into six known subfamilies. Non-canonical RluA variants were also discovered, and subcellular localization of PUS proteins was determined. Transcriptional expression analysis revealed tissue-specific expression and differential responses to heat and salt stresses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hongbo Li, Juan Wei, Yanglan Liao, Xiaoling Cheng, Shuhong Yang, Xiaohong Zhuang, Zhonghui Zhang, Wenjin Shen, Caiji Gao
Summary: This study reveals a previously unidentified function of plant-specific casein kinase I members in attenuating ABA signaling by regulating the phosphorylation and nuclear accumulation of FREE1.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Chen Zhu, Jia-Hui Liu, Jian-Hua Zhao, Ting Liu, Yun-Ya Chen, Chun-Han Wang, Zhong-Hui Zhang, Hui-Shan Guo, Cheng-Guo Duan
Summary: A study has found that the fungal pathogen Verticillium dahliae counters cross-kingdom antifungal RNA interference (RNAi) by secreting a protein called VdSSR1. This protein suppresses the movement of small RNAs (sRNAs) between the host plant and the fungus, increasing the virulence of the fungus in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Peng Xie, Sanyuan Tang, Chengxuan Chen, Huili Zhang, Feifei Yu, Chao Li, Huimin Wei, Yi Sui, Chuanyin Wu, Xianmin Diao, Yaorong Wu, Qi Xie
Summary: Low glume coverage is preferred for easy threshing in grain production, but the genetic basis is unclear. The GC1 gene is found to negatively regulate sorghum glume coverage and naturally truncated alleles can be useful in naked grain breeding. Naked grains with low glume coverage have increased threshing efficiency and seed quality. A strong positive selection signature is found around the GC1 genic region in naked sorghum cultivars.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Guangchao Liu, Jiaxuan Liang, Lijuan Lou, Miaomiao Tian, Xiangyun Zhang, Lijing Liu, Qingzhen Zhao, Ran Xia, Yaorong Wu, Qi Xie, Feifei Yu
Summary: The study reveals that UBP12 and UBP13, as deubiquitinating enzymes, modulate ABA signaling and drought tolerance in Arabidopsis by deubiquitinating and stabilizing VPS23A and the E3 ligase XBAT35.2. This finding demonstrates the important regulatory role of deubiquitination in ABA response.
Editorial Material
Biochemistry & Molecular Biology
Federica Casagrande, Qi Xie, Giovanna Serino
Article
Biochemistry & Molecular Biology
Hongbo Li, Tingting Li, Yingzhu Li, Haiyan Bai, Yanghuan Dai, Yanglan Liao, Juan Wei, Wenjin Shen, Binglian Zheng, Zhonghui Zhang, Caiji Gao
Summary: The FYVE domain protein FREE1 is shown to have a negative regulatory role in plant miRNA biogenesis by interacting with the core microprocessor component in the nucleus.
Article
Multidisciplinary Sciences
Huili Zhang, Feifei Yu, Peng Xie, Shengyuan Sun, Xinhua Qiao, Sanyuan Tang, Chengxuan Chen, Sen Yang, Cuo Mei, Dekai Yang, Yaorong Wu, Ran Xia, Xu Li, Jun Lu, Yuxi Liu, Xiaowei Xie, Dongmei Ma, Xing Xu, Zhengwei Liang, Zhonghui Feng, Xiahe Huang, Hong Yu, Guifu Liu, Yingchun Wang, Jiayang Li, Qifa Zhang, Chang Chen, Yidan Ouyang, Qi Xie
Summary: The use of alkaline salt lands for crop production is limited by a lack of knowledge and breeding efforts in plant alkaline tolerance. Through genome association analysis of sorghum, a major locus, Alkaline Tolerance 1 (AT1), specifically related to alkaline-salinity sensitivity, was identified. Knockout of AT1 increased tolerance to alkalinity in multiple crops, while an at1 allele with a carboxyl-terminal truncation increased sensitivity. AT1 encodes an atypical G protein g subunit that modulates the distribution of hydrogen peroxide (H2O2) by affecting the phosphorylation of aquaporins. Knockout of AT1 homologs or selection of its natural nonfunctional alleles could enhance crop productivity in sodic lands.
Editorial Material
Multidisciplinary Sciences
Wenjing Sun, Huili Zhang, Sen Yang, Lijing Liu, Peng Xie, Jian Li, Yaoyao Zhu, Yidan Ouyang, Qi Xie, Huawei Zhang, Feifei Yu
NATIONAL SCIENCE REVIEW
(2023)
Article
Biochemistry & Molecular Biology
Dapu Liu, Xiaoxing Zhang, Qingliang Li, Yunhua Xiao, Guoxia Zhang, Wenchao Yin, Mei Niu, Wenjing Meng, Nana Dong, Jihong Liu, Yanzhao Yang, Qi Xie, Chengcai Chu, Hongning Tong
Summary: Through yeast two-hybrid screening, we identified a U-box ubiquitin ligase TUD1 that interacts with the negative regulator of BR signaling, GSK2, and promotes its degradation through ubiquitination. The tud1 mutant shows similar phenotypes to plants with constitutively activated GSK2, and GSK2 protein accumulates in the tud1 mutant. In addition, inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability, while BRs induce GSK2 degradation and promote TUD1 accumulation. Our study reveals the role of TUD1 in BR-induced GSK2 degradation and enhances our understanding of a critical step in the BR signaling pathway in rice.
PLANT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jieming Jiang, Xiaolin Ou, Danlu Han, Zhipeng He, Song Liu, Ning Mao, Zhonghui Zhang, Chang-Lian Peng, Jianbin Lai, Chengwei Yang
Summary: This study reveals that localization of the SMC5/6 complex at double-strand breaks in plant cells is dependent on the protein scaffold consisting of IDN2, CDC5, and ADA2b, and this recruitment is further mediated by DNA damage-induced RNAs.
Article
Biotechnology & Applied Microbiology
Ruyi Wang, Xiaoman You, Chongyang Zhang, Hong Fang, Min Wang, Fan Zhang, Houxiang Kang, Xiao Xu, Zheng Liu, Jiyang Wang, Qingzhen Zhao, Xuli Wang, Zeyun Hao, Feng He, Hui Tao, Debao Wang, Jisong Wang, Liang Fang, Mengchao Qin, Tianxiao Zhao, Pingping Zhang, Hefei Xing, Yunping Xiao, Wende Liu, Qi Xie, Guo-Liang Wang, Yuese Ning
Summary: This study constructs a complete E3 ligase library in rice, providing a powerful resource for rapid identification of E3 ligases of ubiquitinated proteins and establishment of functional E3-substrate interactome.