Article
Oncology
Benson Chellakkan Selvanesan, Alvaro de Mingo Pulido, Sheelu Varghese, Deepak Rohila, Daniel Hupalo, Yuriy Gusev, Sara Contente, Matthew D. Wilkerson, Clifton L. Dalgard, Geeta Upadhyay
Summary: This study investigated the effect of a small molecule NSC243928 on intra-tumoral immune cells in mouse mammary tumor models. NSC243928 treatment reduced tumor burden and altered immune cell infiltration. The study suggests that NSC243928 could be a potential drug for immuno-oncology and breast cancer treatment.
Article
Virology
Waqar Ahmad, Neena G. Panicker, Shaima Akhlaq, Bushra Gull, Jasmin Baby, Thanumol A. Khader, Tahir A. Rizvi, Farah Mustafa
Summary: This study aimed to identify genes and pathways dysregulated by MMTV expression in mouse mammary epithelial cells. mRNAseq analysis revealed differentially expressed genes and pathway dysregulation upon MMTV expression. Bioinformatics analysis identified hub genes and dysregulated molecular pathways, including the down-regulation of the PI3-AKT-mTOR pathway. Comparison with other mouse models provided insights into the molecular mechanisms and potential of MMTV to cause cell transformation. These findings have important implications for the understanding of MMTV replication and its involvement in breast cancer.
Article
Biochemistry & Molecular Biology
Seung Un Seo, Seon Min Woo, Shin Kim, Jong-Wook Park, Hyun-Shik Lee, Young-Seuk Bae, Sang Hyun Kim, Seung-Soon Im, Ji Hae Seo, Kyoung-Jin Min, Taeg Kyu Kwon
Summary: Inhibition of cathepsin K enhances oxaliplatin-induced apoptosis by increasing OTUB1 phosphorylation, promoting p53 stabilization, and upregulating Bax expression, leading to decreased tumor size. There is a strong correlation between phosphorylation of OTUB1(Ser16) and p53/Bax expression in human renal clear cell carcinoma tissues.
Article
Immunology
Hai Huang, Xiaonan Wang, Shuling Zhang, Xueli Bai, Noreen Griffin, Yuhua Shan, Fengping Shan
Summary: MENK can significantly inhibit the proliferation, invasion, and migration of MG-63 and Saos-2 cells, arrest the cell cycle in the G0/G1 phase, upregulate Bax, caspase 3, caspase 9, and PARP expression, and downregulate Bcl-2 expression. Tumor size and weight were lower in the MENK group. MENK-treated mice exhibited a reduced ratio of CD11b + Gr-1 + myeloid-derived suppressor cells. MENK increased the ratio of M1-type macrophages and decreased the proportion of M2-type macrophages in tumor tissue. Furthermore, the level of TNF-alpha significantly increased while that of IL-10 decreased in MENK-treated mice. The effect of MENK could be partly reversed by OGFr knockdown.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Anna Matysik-Wozniak, Waldemar A. Turski, Monika Turska, Roman Paduch, Miroslaw Lancut, Pawel Piwowarczyk, Miroslaw Czuczwar, Robert Rejdak
Summary: The study evaluated the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. KYNA showed potential in promoting corneal epithelial cell migration, reducing inflammatory cytokine secretion, and accelerating corneal epithelium healing.
Article
Biochemistry & Molecular Biology
Marion Buffard, Aurelien Naldi, Gilles Freiss, Marcel Deckert, Ovidiu Radulescu, Peter J. Coopman, Romain M. Larive
Summary: Research suggests that SYK can act as an oncogene or tumor suppressor depending on the cell and tissue type. By reconstructing and comparing signaling networks in breast cancer and Burkitt lymphoma cell lines, differences in signaling pathways and network topology were uncovered. Protein interactions were found to be rewired differently in the two types of cancer, demonstrating the complex interplays within SYK pathways affecting tumor formation and progression.
Article
Biochemical Research Methods
Lei Yuan, Shaofang Xie, Huiru Bai, Xiaoqin Liu, Pei Cai, Jing Lu, Chunhui Wang, Zuobao Lin, Shuying Li, Yajing Guo, Shang Cai
Summary: A long-term mammary mini gland culture system derived from adult stem cells has been developed, which can mimic the morphology, size, cellular context, and transcriptional heterogeneity of the normal mammary gland. This system is hormone responsive and can recapitulate the entire postnatal mammary development, as well as initiate tumor formation in vitro and in vivo.
Article
Multidisciplinary Sciences
Hye Kyung Lee, Michaela Willi, Tyler Kuhns, Chengyu Liu, Lothar Hennighausen
Summary: Enhancers that activate gene expression in the lactating mouse mammary gland were investigated, revealing the importance of a lactation-specific distal enhancer and a super-enhancer in coordinating with the promoter for gene activation. The study provides insights into the regulatory mechanisms of enhancers and super-enhancers during lactation.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Geetha Durairaj, Ozlem Demir, Bryant Lim, Robert Baronio, Delia Tifrea, Linda V. Hall, Jacob C. DeForest, Linda Lauinger, Maryam M. Jebril Fallatah, Clinton Yu, Hosung Bae, Da-Wei Lin, Jin Kwang Kim, Faezeh Salehi, Cholsoon Jang, Feng Qiao, Richard H. Lathrop, Lan Huang, Robert Edwards, Scott Rychnovsky, Rommie E. Amaro, Peter Kaiser
Summary: This study utilized a virtual screening approach to identify small molecules that can reactivate the tumor suppressor p53. The UCI-LC0023 compound series was explored in depth and shown to restore the function of mutant p53, prevent tumor progression in cells carrying mutant p53, and induce transcription programs dependent on p53 activity.
CELL CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Teresa Sadras, Mickael Martin, Kohei Kume, Mark E. Robinson, Supraja Saravanakumar, Gal Lenz, Zhengshan Chen, Joo Y. Song, Tanya Siddiqi, Laura Oksa, Anne Marie Knapp, Jevon Cutler, Kadriye Nehir Cosgun, Lars Klemm, Veronika Ecker, Janet Winchester, Dana Ghergus, Pauline Soulas-Sprauel, Friedemann Kiefer, Nora Heisterkamp, Akhilesh Pandey, Vu Ngo, Lili Wang, Hassan Jumaa, Maike Buchner, Jurgen Ruland, Wing-Chung Chan, Eric Meffre, Thierry Martin, Markus Muschen
Summary: Although SYK and ZAP70 kinases share high sequence homology and serve analogous functions, their expression in B and T cells is strictly segregated throughout evolution. Aberrant ZAP70 expression is identified as a common feature in various B cell malignancies. The strict segregation of the two kinases is crucial for normal B cell selection and acts as a central safeguard against autoimmune diseases and B cell malignancies.
Article
Veterinary Sciences
Qiang Li, You-Seok Kim, Ju-Hyun An, Jin-Ah Kwon, Sang-Hyun Han, Woo-Jin Song, Hwa-Young Youn
Summary: In this study, Rivoceranib exhibited anti-proliferative effects on canine melanoma and mammary gland tumor cells, induced cell cycle arrest, decreased tumor volume in animal experiments, induced apoptosis and anti-angiogenic effects in tumor tissues, downregulated cyclin-D1 expression, and inhibited VEGFR2 activity. These findings support the potential use of Rivoceranib as a novel chemotherapeutic strategy for canine melanoma and mammary gland tumors.
BMC VETERINARY RESEARCH
(2021)
Article
Biology
Marion Rosello, Juliette Vougny, Francois Czarny, Marina C. Mione, Jean-Paul Concordet, Shahad Albadri, Filippo Del Bene
Summary: Researchers have successfully generated precise point mutations in zebrafish models using gene editing technology, mimicking oncogenic mutations in human genes and creating new disease models.
Review
Immunology
Lankai Chen, Xipeng Zhang, Guisheng Liu, Shuo Chen, Minying Zheng, Siwei Zhu, Shiwu Zhang
Summary: Mouse mammary tumor virus (MMTV) can be transmitted from mother to offspring through milk during lactation, targeting Peyer's patches (PPs) in the mouse intestine. It enters PPs through microfold cells and activates antigen-presenting cells (APCs) by binding with Toll-like receptors (TLRs). MMTV can infect both APCs and T cells, leading to extensive proliferation and recruitment of immune cells. The infected lymphocytes and dendritic cells in the intestinal lymphatic tissues serve as hotbeds for viral replication and amplification. This transmission of MMTV eventually results in mammary gland infection and the occurrence of breast cancer.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Rebecca M. Harman, Sanjna P. Das, Matt Kanke, Praveen Sethupathy, Gerlinde R. van de Walle
Summary: This study found that the expression level of miR-214-3p in mammosphere-derived epithelial cells affects the occurrence of mammary cancer. In species with low incidence of mammary cancer, the expression of miR-214-3p is lower, leading to increased expression of the apoptosis gene nuclear factor kappa B subunit 1 in response to DNA damage caused by carcinogens.
COMMUNICATIONS BIOLOGY
(2023)
Article
Oncology
Najmeh Alsadat Abtahi, Saba Salehi, Seyed Morteza Naghib, Fatemeh Haghiralsadat, Mohammadmahdi Akbari Edgahi, Sadegh Ghorbanzadeh, Wei Zhang
Summary: MicroRNAs are potential candidates for cancer treatment, and a niosomal delivery system with non-ionic surfactants has been developed for efficient delivery of miR-34a. The system showed successful elimination of cancer cells and reduction in tumor size in both in vitro and in vivo tests, indicating its potential as a tumor suppressor and a reliable procedure for breast cancer treatment.
CANCER NANOTECHNOLOGY
(2023)
Article
Engineering, Multidisciplinary
Tengjiang Yu, Haitao Zhang, Junfeng Sun, Yabo Wang, Shuang Huang, Dan Chen
Summary: The study establishes a generalized formula for the friction coefficient between the base and surface layers of asphalt pavement in cold areas, based on the structural characteristics analysis. The relationships between the generalized friction coefficient and the resilient modulus of asphalt mixtures, temperature shrinkage coefficient, and temperature are also established through quantitative analysis. The findings provide a technical basis for the design, construction, and maintenance of asphalt pavement in cold areas.
JOURNAL OF ENGINEERING DESIGN AND TECHNOLOGY
(2023)
Article
Engineering, Multidisciplinary
Guangyuan Wu, Haitao Zhang, Junfeng Sun, Tengjiang Yu
Summary: The rheological properties of different modified asphalt at different temperatures were comprehensively evaluated using methods such as dynamic shear rheometer and bending beam creep test. The evolution of rheological properties of different modified asphalt was analyzed by analyzing model parameters.
JOURNAL OF ENGINEERING DESIGN AND TECHNOLOGY
(2023)
Article
Physics, Nuclear
Yueling Yang, Liting Wang, Jinshu Huang, Qin Chang, Junfeng Sun
Summary: The study investigates nonleptonic weak decays induced by bottom and charm quark decays in the standard model using a simple factorization approach. The results suggest that several hundred or even thousands of events may be observable in future high-luminosity collider experiments.
Letter
Clinical Neurology
Zhaolin Zhai, Liyuan Ren, Zhenhua Song, Qiong Xiang, Kaiming Zhuo, Suzhen Zhang, Xuan Li, Yi Zhang, Xiong Jiao, Shanbao Tong, Junfeng Sun, Dengtang Liu
Article
Neurosciences
Yujie Ren, Yu Zhang, Jianhua Zhan, Junfeng Sun, Jinhua Luo, Wenqiang Liao, Xing Cheng
Summary: A machine learning-based model was developed to predict postoperative delirium in patients with extensive burns, which was successfully validated. The model can effectively identify high-risk patients and interventions can be made to reduce the incidence of delirium.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Editorial Material
Neurosciences
Kai Yu, Junfeng Sun, Bin He
FRONTIERS IN HUMAN NEUROSCIENCE
(2023)
Article
Genetics & Heredity
Xiaoliang Zhou, Hua Ye, Xianlin Wang, Junfeng Sun, Jiajin Tu, Jing Lv
Summary: Hypertrophic scar is a skin condition characterized by excessive fibrosis with disordered collagens from skin fibroblasts, which causes abnormal esthetic and even functional symptoms, thereby affecting millions of people. Ursolic acid is widely used in skincare and exerts anti-fibrotic effects. The present study aimed to delve into the impact of Ursolic acid on hypertrophic scar and the mechanism.
Review
Critical Care Medicine
Samuel Minkove, Rhea Dhamapurkar, Xizhong Cui, Yan Li, Junfeng Sun, Diane Cooper, Peter Q. Eichacker, Parizad Torabi-Parizi
Summary: This article presents a systematic review of animal studies investigating the effects of lower but still supraphysiologic oxygen concentrations on lung injury. The results suggest that these lower oxygen concentrations might have detrimental effects on animals, particularly in the presence of inflammatory challenges.
INTENSIVE CARE MEDICINE EXPERIMENTAL
(2023)
Article
Chemistry, Multidisciplinary
Mmed. Junfeng Sun, Mmed. Xiaojun Zheng, Mmed. Qiang Gao, Nisar Hussain Lone
Summary: This study investigated the effect of berberine on atherosclerosis-induced damage to the aorta wall in a mouse model. The results showed that berberine treatment significantly reversed the increase in lesion area caused by atherosclerosis. The treatment also reduced the expression of inflammatory markers and targeted the JAK/STAT pathway in atherosclerotic mice.
Article
Multidisciplinary Sciences
Luis J. Perez-Valencia, Kevin M. Vannella, Marcos J. Ramos-Benitez, Junfeng Sun, Mones Abu-Asab, David W. Dorward, Keytam S. Awad, Andrew Platt, Eliana Jacobson, Jason Kindrachuk, Daniel S. Chertow
Summary: Lymphocyte depletion is a key feature of Ebola virus disease, and shed GP, the ectodomain of EBOV glycoprotein, can induce death of B, NK, and T cells. B and NK cells are more susceptible, and the cytotoxic effect starts within 4-5 hours. Shed GP-induced lymphocyte death can occur through caspase-dependent and caspase-independent mechanisms.
Article
Imaging Science & Photographic Technology
Han Wen, Julio A. Huapaya, Shreya M. Kanth, Junfeng Sun, Brianna P. Matthew, Simone C. Lee, Michael Do, Marcus Y. Chen, Ashkan A. Malayeri, Anthony F. Suffredini
Summary: Reduced alveolar volume, changes in vascular and parenchymal structures, along with minimal residual lesions in the lungs, are likely contributors to the lingering low diffusion capacity in some post-COVID-19 patients, according to a cross-sectional study.
JOURNAL OF IMAGING
(2023)
Article
Cell Biology
Xuehua Xu, Xi Wen, Smit Bhimani, Amer Moosa, Dustin Parsons, HyunGee Ha, Tian Jin
Summary: The current dogma states that chemoattractant/G protein-coupled receptors activate beta phospholipase C, while receptor tyrosine kinases activate gamma phospholipase C. This study shows that chemoattractant/G protein-coupled receptor-mediated membrane recruitment of gamma2 phospholipase C is crucial for neutrophil polarization and migration during chemotaxis. Cells lacking gamma2 phospholipase C displayed defects in cell polarization and migration, indicating its essential role in neutrophil chemotaxis.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Correction
Immunology
Sadia H. Sarzynski, Sarah Warner, Junfeng Sun, Roland Matsouaka, John P. Dekker, Ahmed Babiker, Willy Li, Yi Ling Lai, Robert L. Danner, Vance G. Fowler Jr, Sameer S. Kadri
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Engineering, Biomedical
Jie Zhou, Hong Zhu, Jijun Wang, Junfeng Sun
Summary: In this study, the dynamics of local functional connectivity (FC) were explored using the dynamic regional phase synchrony (DRePS) method. The study revealed a consistent spatial distribution of voxels with high or low temporal averaged DRePS in specific brain regions across subjects. The average regional similarity of local FC patterns decreased quickly as volume interval increased, indicating the dynamic change of local FC patterns. The local minimal similarity and the mean of steady similarity were found to have high test-retest reliability and negative correlation with the regional temporal variability of global FC, suggesting the existence of local-to-global FC correlation.
IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING
(2023)
Article
Respiratory System
Seife Yohannes, Nitin Seam, Junfeng Sun, Joel McAlduff, Janet L. Thorne, Susanne B. Lara, Michael Keller
Summary: This study evaluates the impact of implementing an early warning system and response team in medical floors to triage and care for critically ill patients. The results show that the intervention hospital was less likely to admit patients to the ICU and had shorter ICU stays compared to control hospitals. The implementation of the early warning system did not lead to worse outcomes.
CLINICAL MEDICINE INSIGHTS-CIRCULATORY RESPIRATORY AND PULMONARY MEDICINE
(2023)