Article
Food Science & Technology
Haixi You, Yu Zhang, Tianliang Wu, Juanrui Li, Liying Wang, Zhipeng Yu, Jingbo Liu, Xuebo Liu, Long Ding
Summary: This study aimed to investigate the DPP-IV inhibitory peptides from rapeseed proteins. The results showed that rapeseed protein hydrolysates had DPP-IV inhibitory activities. In silico approach was used to select potential DPP-IV inhibitory peptides and 12 of them were determined to have inhibitory activities. Four peptides (IPK, LPR, VPHL, and IPNQT) were identified from rapeseed protein hydrolysate, with IPK exhibiting the highest inhibitory activity. IPK acted as a competitive inhibitor and interacted with specific residues in the S1 and S2 pockets of DPP-IV. These findings suggest that rapeseed proteins are a valuable source of DPP-IV inhibitory peptides for the management of type 2 diabetes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Haixi You, Tianliang Wu, Wei Wang, Yiju Li, Xuebo Liu, Long Ding
Summary: This study found that quinoa protein-derived peptides, particularly QPH1 with molecular weight less than 1 kDa, exhibited strong DPP-IV inhibitory activity. Peptides IPI, IPV, VAYPL, and IPIN showed the highest inhibitory activities and were identified as competitive inhibitors of DPP-IV.
FOOD RESEARCH INTERNATIONAL
(2022)
Review
Chemistry, Medicinal
Anand-Krishna Singh, Dhananjay Yadav, Neha Sharma, Jun-O Jin
Summary: Type 2 diabetes mellitus is characterized by high blood sugar levels, and therapeutic approaches include improving insulin sensitivity and using DPP-IV inhibitors. These inhibitors improve hyperglycemia by stabilizing gut hormones such as glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptides.
Article
Food Science & Technology
Daqiao Yang, Chunsheng Li, Laihao Li, Yueqi Wang, Shengjun Chen, Yongqiang Zhao, Xiao Hu, Hui Rong
Summary: DPP-IV inhibitory peptides from fermented foods like Chouguiyu have the potential to alleviate type 2 diabetes mellitus. This study identified 125 DPP-IV inhibitory peptides in Chouguiyu and selected four novel peptides with the lowest docking energy. These peptides possess structures that easily connect with DPP-IV enzyme through hydrogen bond, salt bridge, and alkyl interactions, and show potential for remission of T2DM.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Food Science & Technology
Long Ding, Ruidi Ma, Haixi You, Juanrui Li, Qingyang Ge, Zhipeng Yu, Liying Wang
Summary: This study investigates the DPP-IV inhibitory properties of wheat gluten-derived peptides. The hydrolysate by flavourzyme showed the highest DPP-IV inhibitory activity, with MPF and VAVPV being the main contributors. Molecular docking revealed that these peptides might interact with the active site of DPP-IV, indicating their potential as ingredients in functional foods or drugs for prediabetes and Type 2 diabetes mellitus management.
JOURNAL OF CEREAL SCIENCE
(2022)
Article
Chemistry, Medicinal
Zongyang Li, Yuan Zhang, Xiangbao Meng, Min Li, Weiwei Cao, Junshan Yang, Xudong Xu, Wenlan Liu, Weiping Li, Qian Cai, Sicen Wang, Guoxu Ma, Zhiheng Liu, Guodong Huang
Summary: The DPP-4 inhibitor Gramcyclin A enhances GLP-1 signaling and shows protective effects against Alzheimer's disease by reversing cognitive decline, reducing abnormal deposits in the brain, suppressing neuroinflammation, and increasing glucose uptake in the brain.
PHYTOTHERAPY RESEARCH
(2022)
Review
Clinical Neurology
Xiaocui Qin, Xia Zhang, Pinyu Li, Min Wang, Li Yan, Zeqing Bao, Qili Liu
Summary: The association between different oral diabetes medications and the risk of Parkinson's disease remains unclear. While the use of sulfonylureas, DPP4 inhibitors, metformin, and glitazones show no significant link to PD risk, metformin and glitazones may still increase the risk. Limited data suggests a protective effect of glucagon-like peptide-1 agonists on the risk of PD.
FRONTIERS IN NEUROLOGY
(2021)
Review
Pharmacology & Pharmacy
Vera Battini, Sara Rocca, Greta Guarnieri, Anna Bombelli, Michele Gringeri, Giulia Mosini, Marco Pozzi, Maria Nobile, Sonia Radice, Emilio Clementi, Antonio Schindler, Carla Carnovale, Nicole Pizzorni
Summary: This study explores the potential effects of three commonly used drug classes on dysphagia and aspiration pneumonia, aiming to probe the possibility of drug repurposing for dysphagia treatment.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Endocrinology & Metabolism
Mona Mashayekhi, Jessica R. Wilson, Scott Jafarian-Kerman, Hui Nian, Chang Yu, Megan M. Shuey, James M. Luther, Nancy J. Brown
Summary: Genetic variation in the gene encoding the GLP-1 receptor may affect the metabolic response to DPP-4 inhibitors. The rs6923761 variant was associated with decreased postprandial glucose excursion in individuals with type 2 diabetes and hypertension. The study suggests a potential role of GLP1R rs6923761 genotype in modulating GLP-1 signaling in response to treatment.
DIABETES OBESITY & METABOLISM
(2021)
Article
Biochemical Research Methods
Phasit Charoenkwan, Chanin Nantasenamat, Md Mehedi Hasan, Mohammad Ali Moni, Pietro Lio, Balachandran Manavalan, Watshara Shoombuatong
Summary: The development of efficient bioinformatics tools for identifying DPP-IV inhibitory peptides is crucial for discovering potential antidiabetic drugs. This study introduces a novel stacking-based ensemble learning predictor, StackDPPIV, which outperforms existing methods by combining different feature encodings and machine learning algorithms. Experimental results demonstrate the superior predictive performance of StackDPPIV on both training and independent datasets.
Article
Endocrinology & Metabolism
Shiau Chin Chong, Norlela Sukor, Sarah Anne Robert, Kim Fong Ng, Nor Azmi Kamaruddin
Summary: This study assessed the impact of endogenous GLP-1 levels on the treatment response to DPP-IV inhibitors in individuals with prediabetes and T2D. The results showed that linagliptin significantly reduced HbA1c and glucose levels in individuals with high GLP-1 levels. This suggests that endogenous GLP-1 levels play an important role in determining the efficacy of DPP-IV inhibitors.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Chemistry, Medicinal
Ponnurengam Malliappan Sivakumar, B. Premkumar, Veluchamy Prabhawathi, Pranav Kumar Prabhakar
Summary: GLP-1RAs have shown significant benefits in improving cardiovascular complications, kidney protection, and neuroprotection in type 2 diabetes mellitus, reducing the risk of therapy.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Samuel Igweokpala, Naheemot Olaoluwa Sule, Antonios Douros, Oriana H. Y. Yu, Kristian B. Filion
Summary: The use of incretin-based drugs is not associated with the risk of diabetic retinopathy among individuals with type 2 diabetes, according to the synthesis of available evidence from observational studies.
DIABETES OBESITY & METABOLISM
(2023)
Article
Endocrinology & Metabolism
Mark M. Smits, Kristina S. Fluitman, Hilde Herrema, Mark Davids, Mark H. H. Kramer, Albert K. Groen, Clara Belzer, Willem M. de Vos, Djuna L. Cahen, Max Nieuwdorp, Daniel H. van Raalte
Summary: The study aimed to investigate the effects of GLP-1 receptor agonists and DPP-4 inhibitors on the intestinal microbiota in adults with type 2 diabetes. Results showed that these agents did not affect the diversity of the intestinal microbiota, nor were changes in microbial composition related to clinical parameters.
DIABETES & METABOLISM
(2021)
Article
Medicine, Research & Experimental
Cristina Bouzas, Rosario Pastor, Silvia Garcia, Margalida Monserrat-Mesquida, Miguel Angel Martinez-Gonzalez., Jordi Salas-Salvado, Dolores Corella, Albert Goday, J. Alfredo Martinez, Angel M. Alonso-Gomez, Olga Fernandez-Barcelo, Jesus Vioque, Dora Romaguera, Jose Lopez-Miranda, Ramon Estruch, Francisco J. Tinahones, Jose Lapetra, Lluis Serra-Majema, Blanca Riquelme-Gallegop, Vicente Martin-Sanchez, Xavier Pinto, Miguel Delgado-Rodriguez, Pilar Matia, Josep Vidal, Jersy-Jair Cardenas-Salas, Lidia Daimiel, Emilio Ros, Estefania Toledo, Josep M. Manzanares, Inmaculada Gonzalez-Monge, Miguel-Angel Munoz, Diego Martinez-Urbistondo, Lucas Tojal-Sierra, Carlos Munoz-Bravoaf, Salvador Miralles-Gisbert, Marian Martin, Antonio Garcia-Rios, Sara Castro-Barquero, Jose Carlos Fernandez-Garcia, Jose Manuel Santos-Lozano, F. Javier Basterra-Gortari, Liliana Gutierrez-Carrasquilla, Patricia Guillem-Saiz, Alba Satorres, Itziar Abete, Carolina Sorto-Sanchez, Javier Diez-Espino, Nancy Babio, Montse Fito, Josep A. Tur
Summary: In a one-year study involving 6881 patients with MetS, it was found that those treated with GLP-1RA and DPP-4I had better glycemic profiles, but improvements in weight and waist circumference were modest.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Anna Jaromin, Robert Zarnowski, Magdalena Pietka-Ottlik, David R. Andes, Jerzy Gubernator
Article
Microbiology
Eddie Dominguez, Robert Zarnowski, Hiram Sanchez, Antonio S. Covelli, William M. Westler, Parastoo Azadi, Jeniel Nett, Aaron P. Mitchell, David R. Andes
Article
Biochemistry & Molecular Biology
Robert Zarnowski, Hiram Sanchez, Antonio S. Covelli, Eddie Dominguez, Anna Jaromin, Joerg Bernhardt, Kaitlin F. Mitchell, Christian Heiss, Parastoo Azadi, Aaron Mitchell, David R. Andes
Article
Microbiology
E. G. Dominguez, R. Zarnowski, H. L. Choy, M. Zhao, H. Sanchez, Jeniel E. Nett, D. R. Andes
Article
Multidisciplinary Sciences
Jieh-Juen Yu, Eric Holbrook, Yu-Rou Liao, Robert Zarnowski, David R. Andes, L. Joseph Wheat, Joshua Malo, Chiung-Yu Hung
Article
Biochemistry & Molecular Biology
Robert Zarnowski, Anna Jaromin, Agnieszka Zagorska, Eddie G. Dominguez, Katarzyna Sidoryk, Jerzy Gubernator, David R. Andes
Summary: The study demonstrated that DiMIQ inhibits Candida albicans biofilm formation by altering fungal cell walls and extracellular matrix, while affecting various metabolic pathways. It may provide a potent alternative for treating biofilm-associated Candida infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hongjie Li, Chang-Yu Sun, Yihang Fang, Caitlin M. Carlson, Huifang Xu, Ana Jesovnik, Jeffrey Sosa-Calvo, Robert Zarnowski, Hans A. Bechtel, John H. Fournelle, David R. Andes, Ted R. Schultz, Pupa U. P. A. Gilbert, Cameron R. Currie
NATURE COMMUNICATIONS
(2020)
Article
Biotechnology & Applied Microbiology
Robert Zarnowski, Hiram Sanchez, Cecilia Andreu, David Andes, Marcel-li del Olmo
Summary: Yeast whole cells are widely used in modern biotechnology for generating compounds of industrial importance. Seawater is now considered a sustainable alternative to freshwater for biocatalytic processes, leading to research on salt-tolerant yeast strains and microbial biofilms. The study characterizes biofilm formation in seawater-based growth media by salt-tolerant yeast strains and provides insights for further development of biocatalytic processes employing seawater and halotolerant yeast biofilms.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Miao Zhao, Fan Zhang, Robert Zarnowski, Kenneth Barns, Ryley Jones, Jen Fossen, Hiram Sanchez, Scott R. Rajski, Anjon Audhya, Tim S. Bugni, David R. Andes
Summary: Turbinmicin is a broad-spectrum antifungal agent that targets biofilm growth. By disrupting extracellular vesicle delivery, turbinmicin impairs biofilm matrix assembly, making drug-resistant biofilms more susceptible to its fungicidal effects. Additionally, turbinmicin can potentiate the activity of other antifungal drugs.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Microbiology
Mark Horton, Chad J. Johnson, Robert Zarnowski, Brody D. Andes, Taylor J. Schoen, John F. Kernien, Douglas Lowman, Michael D. Kruppa, Zuchao Ma, David L. Williams, Anna Huttenlocher, Jeniel E. Nett
Summary: Candida auris, an emerging fungal pathogen with high mortality rates, evades neutrophil activity through cell wall mannosylation, a mechanism distinct from other common Candida species. Disruption of mannosylation pathways renders C. auris susceptible to neutrophil killing and sheds light on innate immune evasion for this emerging pathogen.
Review
Biotechnology & Applied Microbiology
Cecilia Andreu, Robert Zarnowski, Marceluli del Olmo
Summary: This study summarizes the latest findings on halotolerant yeasts and provides a detailed description of their modern biotechnological applications.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Robert Zarnowski, Andrea Noll, Marc G. Chevrette, Hiram Sanchez, Ryley Jones, Hanna Anhalt, Jen Fossen, Anna Jaromin, Cameron Currie, Jeniel E. Nett, Aaron Mitchell, David R. Andes
Summary: The fungal pathogen Candida albicans can form biofilms that protect it from drugs and the immune system by releasing extracellular vesicles (EVs) that promote extracellular matrix formation and resistance to antifungal drugs. The study identifies functions for numerous EV cargo proteins in biofilm matrix assembly, drug resistance, fungal cell adhesion, and dissemination. The findings suggest that EVs play a crucial role in coordinating biofilm development in C. albicans.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Robert Zarnowski, Hiram Sanchez, Anna Jaromin, Urszula J. Zarnowska, Jeniel E. Nett, Aaron P. Mitchell, David Andes
Summary: Extracellular vesicles play an important role in mediating community interactions among cells. In this study, researchers investigated the composition and function of biofilm-associated vesicles in different Candida species. They found similarities in vesicle size and release over the biofilm lifespan, and identified a group of proteins enriched in these vesicles that are important for biofilm processes. By delivering wild-type vesicle cargo, they were able to restore mutant phenotypes and observed functional complementation in cross-species biofilm interactions. These findings highlight the significance of vesicles in shaping protective biofilm communities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Robert Zarnowski, Justin Massey, Aaron P. Mitchell, David Andes
Summary: Extracellular vesicles play a crucial role in modulating interactions between cellular communities. They are involved in delivering core protein and carbohydrate cargo, which is essential for biofilm maturation features such as matrix production, drug resistance, and dispersion in Candida species. While vesicle cargo promotes cooperative interactions within species, it exhibits interference competition among different Candida species in mixed-species biofilms. This study highlights the dual roles of vesicle cargo in biofilm initiation, serving as a negative factor during cross-species interactions but supporting the growth of mature communities.
Article
Microbiology
Max P. Cravener, Eunsoo Do, Gemma May, Robert Zarnowski, David Andes, C. Joel McManus, Aaron Mitchell
Summary: Biofilm growth of the fungal pathogen Candida albicans is a prevalent source of infection. Deletion of the master regulatory gene EFG1 results in abolishment of biofilm formation in all isolates of different clades and infection sites. A principal gene set of Efg1-responsive genes has been identified, including direct and indirect targets. Upregulation of the transcription factors BRG1, UME6, and WOR3, among the direct Efg1 targets, restores the expression of multiple principal targets and biofilm formation ability. Wor3, known as an antagonist of Efg1, unexpectedly promotes biofilm formation.