Article
Multidisciplinary Sciences
Daisuke Kitagawa, Yuki Suzuki, Noriyuki Abe, Koji Ui, Keitaro Suzuki, Takaya Yamashita, Atsuko Sakaguchi, Soma Suzuki, Kazue Masuo, Akiyo Nakano, Ryuichi Nakano, Masatoshi Sato, Koichi Maeda, Fumihiko Nakamura, Hisakazu Yano
Summary: In this study, the accuracy of two different bacterial identification methods, rpoB gene sequencing in conjunction with phylogenetic analysis and MALDI-TOF MS, were compared for differentiating Aeromonas spp. The results showed that rpoB sequencing method was the most accurate for species level identification, while MALDI Biotyper and VITEK MS accurately identified isolates at the genus level. However, MALDI-TOF MS-based methods had the advantages of low cost and ease of analysis, making them suitable for use in clinical laboratories.
Article
Biotechnology & Applied Microbiology
Samart Dorn-In, Sirkka Mang, Karin Schwaiger
Summary: This study investigated cold-tolerant Clostridium spp. strains isolated from various samples, identifying them into different groups based on genetic and biochemical characteristics. Spiked beef samples showed changes in odor after storage, while maintaining similar color and meat quality compared to the control group.
Article
Agriculture, Dairy & Animal Science
Leszek Guz, Aneta Nowakiewicz, Krzysztof Puk, Przemyslaw Zieba, Sebastian Gnat, Lukasz Matuszewski
Summary: The study identified a high prevalence of Aeromonas species in water sources and European pond turtles from Eastern Poland, with most isolates showing resistance to multiple antibiotics and carrying multiple virulence genes.
Article
Food Science & Technology
Josie Roberta Teodoro, Gabriela Guimaraes Carvalho, Murilo Mariz Queiroz, Carlos Emilio Levy, Dirce Yorika Kabuki
Summary: Aeromonas spp. is an opportunistic bacterium that can cause infections in animals and humans. This study investigated the prevalence, virulence profile, and antimicrobial resistance of Aeromonas spp. in ready-to-eat foods. The results showed that temaki, a type of sushi, is a carrier of potentially pathogenic Aeromonas spp. and should be avoided by certain vulnerable populations. Constant monitoring of these microorganisms is necessary due to the presence of antimicrobial-resistant strains.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Fisheries
Gaixiao Qin, Jin Xu, Xiaohui Ai, Yibin Yang
Summary: This study isolated a pathogenic strain ZZ051 from hemorrhagic channel catfish and identified it as Aeromonas veronii using physiological and biochemical methods, 16S rRNA gene sequencing, and MALDI-TOF-MS analysis. The isolated strain was confirmed to be the causative agent of the catfish disease through artificial infection. It was highly sensitive to enrofloxacin but resistant to florfenicol. This research provides a theoretical basis for preventing and controlling hemorrhagic disease in channel catfish.
Article
Microbiology
Luna Girolamini, Maria Rosaria Pascale, Marta Mazzotta, Simona Spiteri, Federica Marino, Silvano Salaris, Antonella Grottola, Massimiliano Orsini, Sandra Cristino
Summary: Legionella surveillance is crucial for preventing infection risks and studying the ecology of isolates, but difficulties in isolation may lead to underestimation of human infection. The discovery of a new Legionella species highlights the importance of paying attention to characteristics of isolates less associated with the Legionella genus during culture techniques.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Daniele Maubon, Cecile Garnaud, Lala Soavina Ramarozatovo, Rapelanoro Rabenja Fahafahantsoa, Muriel Cornet, Tahinamandranto Rasamoelina
Summary: Chromoblastomycosis and sporotrichosis, as neglected tropical diseases, have laboratory diagnosis methods that require expertise and have limitations in sensitivity and specificity. However, the application of molecular methods such as MALDI-TOF MS, PCR, and sequencing can greatly improve the speed and accuracy of diagnosis, providing user-friendly point-of-care tests for basic healthcare settings and field consultations.
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Article
Microbiology
Mari Tohya, Kanae Teramoto, Shin Watanabe, Tomomi Hishinuma, Masahito Shimojima, Miho Ogawa, Tatsuya Tada, Yoko Tabe, Teruo Kirikae
Summary: The study compared different methods to identify Pseudomonas species and found that many of the clinical isolates, initially identified as P. putida or P. fluorescens, were misidentified. Whole-genome sequencing (WGS) and MALDI-TOF MS analysis revealed that these isolates actually belonged to different Pseudomonas species, including 9 novel species. The study suggests that WGS is the optimal method for Pseudomonas species identification, and MALDI-TOF MS has the potential to complement WGS.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Mary Cruz Torrico, Anna Fernandez-Arevalo, Cristina Ballart, Marco Solano, Ernesto Rojas, Alba Abras, Fabiola Gonzales, Albert Arnau, Silvia Tebar, Teresa Llovet, Daniel Lozano, Eva Ariza-Vioque, Joaquim Gascon, Albert Picado, Faustino Torrico, Carmen Munoz, Montserrat Gallego
Summary: The objective of the study was to evaluate the usefulness of MALDI-TOF MS for the characterization of Leishmania species in Bolivia. All of the isolates could be identified, and no misidentifications were observed at the complex level. MALDI-TOF MS can be used as an alternative to molecular techniques for the identification of Leishmania spp., and its simplicity and cost-effectiveness make it a valuable tool in improving patient management and control of tegumentary leishmaniasis in Bolivia.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Lijun Zhao, Lirong Jia, Bingcun Ma, Wei Zhong, Ying Huang, Feixia Duan
Summary: The study found that temperature significantly affects the texture stiffening of soybean curd, with high-temperature autoclaving leading to increased hardness. Analysis identified Bacillus, Clostridium, Lysinibacillus, and Brevibacillus as main contaminants. Bacterial contamination in two different processing lines was dominated by Staphylococcus, Macrococcus, Psychrobacter, Bacillus, and Acinetobacter.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Microbiology
Ekaterina Frantsuzova, Alexander Bogun, Anna Vetrova, Yanina Delegan
Summary: Gordonia spp. are common pathogens found in soil, but their identification and susceptibility to antimicrobial drugs are not well understood. This review discusses the methods for identifying Gordonia strains and presents future directions in this field.
Article
Microbiology
Arkadiusz Jozef Zakrzewski, Urszula Zarzecka, Wioleta Chajecka-Wierzchowska, Anna Zadernowska
Summary: This study aimed to compare the effectiveness of different identification methods for Enterobacterales strains isolated from fresh fish and shrimps. The results showed that conventional identification methods could provide false results, while the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) had the highest genus identification rate.
Article
Environmental Sciences
Samet Kalkan
Summary: This study focused on the marine sediments in the Black Sea and explored the value of bacterial diversity for public health and biotechnology applications. Through the collection of sediment samples and the use of multimodal analytical platforms, 26 different bacterial species were identified, including some newly reported species. These findings contribute to a better understanding of microbial life in the southeastern sediments of the Black Sea.
MARINE POLLUTION BULLETIN
(2022)
Article
Agriculture, Dairy & Animal Science
Esther Vazquez-Fernandez, Blanca Chinchilla, Agustin Rebollada-Merino, Lucas Dominguez, Antonio Rodriguez-Bertos
Summary: This article discusses a mortality outbreak in sturgeons caused by Aeromonas salmonicida in Spain. The study found that sturgeons are susceptible to this pathogen and exhibit a hyperacute form of the disease without specific clinical signs. The findings contribute to a better understanding of aeromoniasis in sturgeons and its impact on the aquaculture and caviar industries.
Article
Medical Laboratory Technology
Myungsook Kim, Shin Young Yun, Yunhee Lee, Hyukmin Lee, Dongeun Yong, Kyungwon Lee
Summary: This study investigated clinical differences among patients infected with Fusobacterium spp. and determined the antimicrobial susceptibility of the isolates. Despite clinical differences, there was no significant difference in mortality rates among patients infected with different species of Fusobacterium. Some Fusobacterium spp. exhibited resistance to certain commonly used antimicrobial agents.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Junhyup Song, Shinyoung Yoon, Yongha In, Daewon Kim, Hyukmin Lee, Dongeun Yong, Kyoungwon Lee
Summary: This study evaluated the performance of MALDI-TOF MS in identifying nontuberculous mycobacteria (NTM) and achieved improved identification results through an upgraded database. The study laid the foundation for further application of MALDI-TOF MS in the rapid identification of NTM.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Pediatrics
Seungbok Lee, Se Song Jang, Soojin Park, Jihoon G. Yoon, Soo Yeon Kim, Byung Chan Lim, Jong Hee Chae
Summary: This study investigated the clinical features of patients with CTNNB1-related neurodevelopmental disorder and found that patients showed varying degrees of intellectual disability, impaired motor performance, and ophthalmologic problems. In addition, female patients exhibited autistic features.
FRONTIERS IN PEDIATRICS
(2022)
Article
Genetics & Heredity
Soojin Park, Se Song Jang, Seungbok Lee, Minsoo Kim, Hyungtai Sim, Hyeongseok Jeon, Sung Eun Hong, Jean Lee, Jeongeun Lee, Eun Young Jeon, Jeongha Lee, Cho-Rong Lee, Soo Yeon Kim, Man Jin Kim, Jihoon G. Yoon, Byung Chan Lim, Woo Joong Kim, Ki Joong Kim, Jung Min Ko, Anna Cho, Jin Sook Lee, Murim Choi, Jong-Hee Chae
Summary: By analyzing the genetic characteristics of 1,180 Korean patients with neurological symptoms, we identified different genetic mechanisms and expression patterns for genes involved in neurodevelopmental disorders.
FRONTIERS IN GENETICS
(2022)
Letter
Clinical Neurology
Jae Young Joo, Jihoon G. Yoon, Seoungbok Lee, Jangsup Moon, Han-Joon Kim
MOVEMENT DISORDERS
(2023)
Article
Multidisciplinary Sciences
Yong Chan Kim, Heun Choi, Young Ah Kim, Yoon Soo Park, Young Hee Seo, Hyukmin Lee, Kyungwon Lee
Summary: Understanding the risk factors and microbiological features in recurrent E. coli BSI can aid clinicians in their management planning. Data from 2017 to 2018 were collected to assess antimicrobial resistance rates and molecular epidemiology. Risk factors for recurrence included vascular catheterization, ESBL phenotype, and high SOFA score. The highest proportion of ST131 and ESBL genotype was observed in early onset recurrent BSI. Whole genome sequencing revealed the presence of various virulence factors, resistant genes, and plasmid types in all types of BSI. This knowledge is important for clinicians in managing recurrent E. coli BSI.
Letter
Medical Laboratory Technology
Jihoon G. Yoon, Seungbok Lee, Sheehyun Kim, Man Jin Kim, Yoon Hwan Chang, Jin Kyun Park, Dong-Yeop Shin, Jangsup Moon
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Yangsoon Lee, Hye Gyung Bae, Dongju Won, Woobin Yun, Hyukmin Lee, Jong Rak Choi, Young Uh, Kyungwon Lee
Summary: In this study, the distribution of GBS isolates in pregnant Korean women was investigated. CC1 was the dominant lineage, followed by CC19, CC12, and CC17. The low prevalence of CC17 GBS colonization may explain the low frequency of neonatal GBS infections. Whole-genome sequencing is a useful tool for predicting antimicrobial susceptibility and capsular serotypes in GBS isolates.
ANNALS OF LABORATORY MEDICINE
(2023)
Letter
Clinical Neurology
Hyoshin Son, Jihoon G. Yoon, Man Jin Kim, Jangsup Moon, Han-Joon Kim
JOURNAL OF MOVEMENT DISORDERS
(2023)
Article
Genetics & Heredity
Man Jin Kim, Sheehyun Kim, Seung Won Chae, Seungbok Lee, Jihoon G. Yoon, Boram Kim, Jee-Soo Lee, Jong Hee Chae, Moon-Woo Seong, Jangsup Moon
Summary: This study found that DRC1 exon 1-4 deletion is a common mutation in PCD patients in Korea and Japan, possibly due to a founder effect. It is suggested that genetic testing for DRC1 exon 1-4 deletion should be considered as an initial screening tool for PCD patients in Korea and Japan.
JOURNAL OF HUMAN GENETICS
(2023)
Article
Immunology
Han Sang Kim, Chang Gon Kim, Won Kyu Kim, Kyung-A Kim, Jinseon Yoo, Byung Soh Min, Soonmyung Paik, Sang Joon Shin, Hyukmin Lee, Kyungwon Lee, Hoguen Kim, Eui-Cheol Shin, Tae-Min Kim, Joong Bae Ahn
Summary: This study found that Fusobacterium nucleatum (FN) in colorectal cancer tissues is associated with immune regulation in the tumor microenvironment, and its infection is correlated with poor prognosis and altered distribution of immune cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Medicine, General & Internal
Jihoon G. Yoon, Man Jin Kim, Yong Jin Kwon, Jong-Hee Chae
Summary: Next-generation sequencing (NGS) technologies have greatly impacted genetic testing in clinical settings, but proper variant interpretation and clinical evaluation are essential for optimal clinical practice. Ethical considerations and regulatory measures are also necessary to prepare for the future of genomic medicine.
JOURNAL OF THE KOREAN MEDICAL ASSOCIATION
(2023)
Article
Clinical Neurology
Young Jun Ko, Soo Yeon Kim, Seungbok Lee, Jihoon G. Yoon, Man Jin Kim, Hyeji Jun, Hunmin Kim, Jong-Hee Chae, Ki Joong Kim, Kwangsoo Kim, Byung Chan Lim
Summary: This study aimed to identify the clinical and genetic characteristics of patients with epilepsy in an NDD cohort and demonstrate the importance of genetic testing. The results showed no significant differences in clinical features and treatment responses between NDD patients with epilepsy caused by epilepsy-related genes and those caused by NDD-related genes. Further studies are needed to understand the integrated pathomechanisms.
FRONTIERS IN NEUROLOGY
(2023)
Letter
Clinical Neurology
Jaeso Cho, Jihoon G. Yoon, Seungbok Lee, Sheehyun Kim, Soo Yeon Kim, Man Jin Kim, Jangsup Moon, Jong-Hee Chae
Summary: Cho et al. identified novel variants in two Korean ataxia patients, expanding the clinico-genomic findings of PRDX3 disease. These pathogenic PRDX3 variants, found in compound heterozygotes with a previously reported variant, highlight the need for increased awareness of PRDX3 disease in East Asians.
BRAIN COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Shin Young Yun, Yunhee Lee, Juwon Hong, Dong-Chan Kim, Hyukmin Lee, Dongeun Yong, Yun Kyong Lim, Joong-Ki Kook, Kyungwon Lee
Summary: The aim of this study was to improve the accuracy and efficacy of identifying Fusobacterium species in clinical laboratories. Through the identification of 229 Fusobacterium isolates, the updated ASTA MicroIDSys system was found to be able to effectively identify nine species of Fusobacterium and four subspecies of F. nucleatum.
YONSEI MEDICAL JOURNAL
(2022)