Article
Biochemistry & Molecular Biology
Alexander J. Knights, Shanshan Liu, Yingxu Ma, Victoria S. Nudell, Eric Perkey, Matthew J. Sorensen, Robert T. Kennedy, Ivan Maillard, Li Ye, Heejin Jun, Jun Wu
Summary: This study demonstrates that macrophages play a crucial role in regulating thermogenesis by secreting acetylcholine in adipose tissue. The abundance of ChAMs increases in response to acute cold exposure, and their regulation is mediated through adrenergic signaling. Deletion of the enzyme ChAT impairs cold-induced thermogenesis in mice.
Article
Biochemistry & Molecular Biology
Moema de Alencar Hausen, Ariana de Souza Moraes, Flavia Pedrini, Felipe Grabarz, Jose Angelo Camilli, Eliana Aparecida de Rezende Duek
Summary: This study introduces a natural biomaterial device that can improve the wound healing process by modulating macrophage behavior in vitro. The device, assembled with adipose-derived stem cells and biocompatible polymers, promotes the release of anti-inflammatory cytokines, indicating the prevalence of M2 macrophages.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hsu-Hsiang Lin, Pen-Hsiu Grace Chao, Wei-Chiu Tai, Po-Chun Chang
Summary: This study successfully developed a biomimetic microfibrous system to assist PDL regeneration and withstand functional load. Collagen-based microfibers were fabricated and PDL cells seeded on waveform microfibers showed enhanced growth and gene expression levels, indicating potential for promoting healing and regeneration under shear stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Pavel Hruska, Jan Kucera, Matej Pekar, Pavol Holeczy, Miloslav Mazur, Marek Buzga, Daniela Kuruczova, Peter Lenart, Jana Fialova Kucerova, David Potesil, Zbynek Zdrahal, Julie Bienertova-Vasku
Summary: The study compared the proteomic profiles of mature adipocytes from different adipose tissue depots, with findings showing that subcutaneous adipocytes are more active in vesicular transport, secretion, and lipid metabolism, while visceral adipocytes have higher protein expression related to mitochondrial energy metabolism and translational or biosynthetic activity.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Francesca Favaretto, Chiara Compagnin, Elisa Cogliati, Giulia Montagner, Francesco Dell'Antonia, Giorgio Berna, Roberto Vettor, Gabriella Milan, Diletta Trojan
Summary: This study aimed to characterize and validate the storage effectiveness of cryopreserved adipose tissue (AT) for up to three years for clinical applications. AT components were isolated from fresh and cryopreserved samples and cell viability was assessed. Adipogenic and endothelial precursors and mature cells were measured. The differentiation potential and cellular composition of cryopreserved AT were maintained. Two patients successfully underwent autologous cryopreserved AT transplants without complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Jingyi Chi, Zeran Lin, William Barr, Audrey Crane, Xiphias Ge Zhu, Paul Cohen
Summary: The study revealed that the association between beige adipocytes and dense sympathetic neurites in mouse subcutaneous fat is established during early postnatal period, with Prdm16 playing a crucial role in guiding sympathetic growth. However, this patterning is not essential for maintaining the sympathetic structure in mature animals.
Article
Agriculture, Multidisciplinary
Xin Wang, Hao Cai, Shanshan Shui, Yan Lin, Fangbin Wang, Lu Wang, Juan Chen, Jian Liu
Summary: Chrysin, a natural flavonoid, alleviates adiposity and insulin resistance in mice fed a high-fat diet. It promotes subcutaneous adipocyte browning and systemic energy expenditure by regulating PDGFR alpha and microRNA expressions in the SAT.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Shuqing Yang, Wenwei Jiang, Xi Hong, Peixian Chen, Wei Li, Guangyu Yao, Dan Zhou
Summary: This study developed a gelatin-based bilayer porous scaffold for subcutaneous osteochondral regeneration, using the antiangiogenic drug curcumin and the proangiogenic drug Danshen. The results showed that the curcumin-loaded gelatin inhibited tube formation, while the Danshen-loaded gelatin promoted tube formation. The bilayer scaffold successfully achieved subcutaneous osteochondral regeneration when recolonized with bone marrow stem cells.
Article
Cell Biology
Hai P. Nguyen, Frances Lin, Danielle Yi, Ying Xie, Jennie Dinh, Pengya Xue, Hei Sook Sul
Summary: Adipose tissue mass and adiposity change throughout lifespan, with visceral adipose tissue (VAT) increasing and subcutaneous adipose tissue (SAT) decreasing with aging. A regulatory cell population called ARCs emerges in aging SAT, expressing progenitor markers but lacking adipogenic capacity, inhibiting proliferation and differentiation of neighboring adipose precursors. Pu.1 is identified as a driving factor for ARC development, correlating with aging-associated loss of SAT.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Kyung-A Byun, Hyun Jun Park, Seyeon Oh, Sosorburam Batsukh, Hye Jin Sun, Taehui Kim, Sunggeun Kim, Donghwan Kang, Kuk Hui Son, Kyunghee Byun
Summary: High-intensity focused ultrasound (HIFU) decreases subcutaneous adipose tissue (SAT) thickness through inducing adipocyte necrosis. The study found that HIFU increases the expression of nuclear and mitochondrial p53, leading to apoptosis and autophagy. HIFU also affects the expression of apoptosis and autophagy-related proteins, resulting in decreased SAT thickness.
Article
Nanoscience & Nanotechnology
Matthew McGrath, Karolina Zimkowska, Katelyn J. Genoud, Jack Maughan, Javier Gutierrez Gonzalez, Shane Browne, Fergal J. O'Brien
Summary: A biomimetic, bilayered antimicrobial collagen-based scaffold was developed to deal with the etiology of diabetic foot ulcers (DFUs). The scaffold exhibited high structural stability, successfully inhibited the growth and infiltration of Staphylococcus aureus, and supported the proliferation of epidermal cells and vascularization. These results suggest that the bilayered scaffold is a promising candidate for enhancing diabetic wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Panneerselvam Jithendra, Jamal Moideen Muthu Mohamed, Dinesh Annamalai, Rasha Hamed Al-Serwi, Ateya Megahed Ibrahim, Mohamed El-Sherbiny, Abraham Merlin Rajam, Mamdouh Eldesoqui, Nimer Mansour
Summary: The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes. The scaffold showed higher chondrogenic efficacy compared to COL-CS, making it a suitable candidate for cartilage tissue engineering. The study utilized various assays and staining techniques to analyze cytocompatibility, GAG content, RNA content, and expression of cartilage-specific genes and collagen type II.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Editorial Material
Endocrinology & Metabolism
Imke L. Lemmer, Alexander Bartelt
Summary: An elegant quantitative analysis of brown fat and skeletal muscle metabolite flux reveals that brown fat predominantly uses glucose and lactate and acts as a nitrogen scavenger during thermogenesis.
Article
Chemistry, Applied
Sihan Zhang, Guanglei Zhao, Wei Ma, Yanghui Song, Cheng Huang, Chong Xie, Kebing Chen, Xiaofeng Li
Summary: A porous chitosan nanofiber scaffold (CSNFS) with an extracellular matrix-like structure was prepared through a cross-linking reaction, showing high porosity and adequate micro-scale pores. CSNFS has good mechanical strength and biocompatibility, and can be used as a repair material for bone tissue engineering.
CARBOHYDRATE POLYMERS
(2022)
Article
Anatomy & Morphology
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi
Summary: Tissue engineering research has focused on the characteristics and biocompatibility of decellularized sheep tracheal scaffolds, as well as the differentiation of Adipose-derived stem cells (AD-MSCs) into tracheal cells. The prepared scaffolds show potential for engineering applications and provide a suitable environment for stem cell differentiation. This research serves as a foundation for future studies on tracheal decellularization scaffolds and paves the way for their use in organ regeneration and clinical medicine.