Article
Biochemistry & Molecular Biology
Robert Soukup, Iris Gerner, Sinan Gueltekin, Hayeon Baik, Johannes Oesterreicher, Johannes Grillari, Florien Jenner
Summary: Extracellular vesicles (EVs) play an essential role in cellular crosstalk, and equine MSC-derived EVs have comparable therapeutic potential to their donor cells. The International Society for Extracellular Vesicles (ISEV) has proposed standards to define EVs and their functions, but their application in veterinary field is restricted by nonstandardised isolation methods and limited availability of cross-reacting markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Alison J. Wilson, Emma Rand, Andrew J. Webster, Paul G. Genever
Summary: Many clinical trials involving mesenchymal stem/stromal cells lack comprehensive characterization data of the cellular population, including details on surface marker expression, viability, and differentiation. Functional assessment is very limited and does not always correlate with the likely mechanism of action.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Gautier Tejedor, Patricia Luz-Crawford, Audrey Barthelaix, Karine Toupet, Sebastien Roudieres, Francois Autelitano, Christian Jorgensen, Farida Djouad
Summary: The research demonstrates that MSC derived from MRL mice have a higher migration potential and produce higher levels of MANF, which plays a significant role in regenerative medicine and OA treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chiara Reina, Clara Cardella, Margot Lo Pinto, Gaia Pucci, Santina Acuto, Aurelio Maggio, Vincenzo Cavalieri, Antonino Germana
Summary: Conditioned media derived from stem cell culturing have high potential as therapeutic tools against various diseases. Zebrafish embryos were used to evaluate the biological effects of a conditioned medium derived from Wharton's jelly mesenchymal stem cells. The exposure to the conditioned medium triggered antioxidant, anti-apoptotic, and pro-regenerative effects through gene upregulation and downregulation, leading to future therapeutic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Weizong Wang, Yixin Zhao, Huilin Li, Yujiao Zhang, Xiaomeng Jia, Cong Wang, Pengju Zhu, Jiangrong Wang, Yinglong Hou
Summary: This study demonstrates that MSC-derived exosomes can enhance the proliferation, migration, and tube formation ability of umbilical vein endothelial cells (HUVECs) in vitro, as well as inhibit apoptosis induced by rapamycin. Furthermore, a distinct class of exosomal miRNAs, including six miRNAs closely related to neovasculogenesis, was identified.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Cell Biology
Candice Bohaud, Rafael Contreras-Lopez, Jholy de la Cruz, Claudia Terraza-Aguirre, Mingxing Wei, Farida Djouad, Christian Jorgensen
Summary: Osteoarthritis is a multifaceted degenerative and inflammatory joint disorder, with current therapies often only providing short-term benefits without complete structural and functional reconstitution. Identifying novel therapeutic targets and strategies is crucial to achieve long-term clinical benefits for OA patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell & Tissue Engineering
Len Frisbie, Ronald J. Buckanovich, Lan Coffman
Summary: The interaction between tumor cells and non-malignant host cells within the tumor microenvironment (TME) is crucial for cancer progression. Mesenchymal stem/stromal cells (MSCs), one of the non-malignant host cells, play a critical role in the formation and function of the TME. These MSCs, when reprogrammed by cancer cells, become carcinoma-associated MSCs (CA-MSCs) and contribute to tumor growth, metastasis, and TME formation. CA-MSCs can differentiate into various stromal sub-lineages, secrete extracellular matrix, promote angiogenesis, and recruit immunosuppressive myeloid cells, thereby impacting almost every aspect of the TME. However, the heterogeneity of CA-MSCs and the lack of definitive markers for their identification pose challenges in accurately understanding their origin and characteristics.
Review
Biochemistry & Molecular Biology
Kian Leong Tan, Wui Chuen Chia, Chee Wun How, Yin Sim Tor, Pau Loke Show, Qi Hao Daniel Looi, Jhi Biau Foo
Summary: The study aimed to develop a simple protocol for isolating and characterizing sEVs from human umbilical cord-derived MSCs. Morphological, immunophenotypic, and multidifferentiation analyses were conducted on hUC-MSCs. The study successfully isolated sEVs from the conditioned medium of hUC-MSCs and characterized their size distribution, surface marker population, and internal markers. Further exploration of the downstream application of hUC-MSCs-sEVs is planned.
MOLECULAR BIOTECHNOLOGY
(2021)
Article
Cell Biology
Felicia A. M. Klampfleuthner, Benedict Lotz, Tobias Renkawitz, Wiltrud Richter, Solvig Diederichs
Summary: PI3K/AKT signaling plays an important role in the speed of chondrogenesis in human mesenchymal stromal cells (MSCs), but not in hypertrophic degeneration. This study provides valuable insights for improving MSC chondrogenesis and generating high-quality cartilage.
Article
Multidisciplinary Sciences
Maria Casanova-Acebes, Erica Dalla, Andrew M. Leader, Jessica LeBerichel, Jovan Nikolic, Blanca M. Morales, Markus Brown, Christie Chang, Leanna Troncoso, Steven T. Chen, Ana Sastre-Perona, Matthew D. Park, Alexandra Tabachnikova, Maxime Dhainaut, Pauline Hamon, Barbara Maier, Catherine M. Sawai, Esperanza Agullo-Pascual, Markus Schober, Brian D. Brown, Boris Reizis, Thomas Marron, Ephraim Kenigsberg, Christine Moussion, Philippe Benaroch, Julio A. Aguirre-Ghiso, Miriam Merad
Summary: Macrophages play a crucial role in shaping the tumor microenvironment and tumor immunity, with tissue-resident macrophages identified as key contributors to early lung cancer progression. Targeting tissue-resident macrophages may offer new possibilities for prevention and treatment of early lung cancer lesions.
Review
Biochemistry & Molecular Biology
Youssef Slama, Franck Ah-Pine, Mohamed Khettab, Angelique Arcambal, Mickael Begue, Fabien Dutheil, Philippe Gasque
Summary: Mesenchymal stem/stromal cells (MSCs) play a dual role in cancer pathophysiology by interacting with the tumor microenvironment and influencing tumor cells. They can promote tumor progression through various mechanisms while also exhibiting therapeutic effects such as anti-tumor and anti-inflammatory effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Agriculture, Dairy & Animal Science
Soheil Sadr, Narges Lotfalizadeh, Abolfazl Ghaniei, Pouria Ahmadi Simab, Ashkan Hajjafari
Summary: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into various cell types and produce growth factors and cytokines. They play important roles in bone mineralization, infectious diseases, and interactions with other cells. MSCs have advantages as a stem cell model, including availability, proliferation, and immune-modulatory properties.
WORLDS POULTRY SCIENCE JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Yoshitaka Iso, Sayaka Usui, Hiroshi Suzuki
Summary: The aim of this study was to investigate the pro-angiogenic potential of skeletal muscle mesenchymal stem/stromal cells (mMSCs). The results showed that PDGFR-alpha positive mMSCs secreted VEGF and hepatocyte growth factor, inducing endothelial tube formation and promoting capillary growth. Epo stimulation enhanced phosphorylation and cellular proliferation in mMSCs, leading to improved perfusion recovery and capillary growth in the ischemic limbs.
Article
Cell Biology
Cosette M. Rivera-Cruz, Shreya Kumar, Marxa L. Figueiredo
Summary: In this study, we primed human MSC derived from adipose tissues using toll-like receptor agonists and investigated their interactions with prostate cancer cells. The results showed that primed MSC significantly accelerated tumor growth and induced changes in the tumor microenvironment, including altered modulation of angiogenesis and immune response processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nerea Palao, Celia Sequera, Angel M. Cuesta, Cristina Baquero, Paloma Bragado, Alvaro Gutierrez-Uzquiza, Aranzazu Sanchez, Carmen Guerrero, Almudena Porras
Summary: The knock-down of C3G enhances the migratory and invasive ability of oval cells and increases the expression of stemness markers. It also enhances the clonogenic capacity of oval cells. The knock-down of C3G impairs HGF/MET signaling and increases TGF-beta signaling in oval cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
