Journal
PLASMID
Volume 70, Issue 2, Pages 240-246Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2013.05.001
Keywords
Symbiosis; Vitamin C; L-ascorbic acid; Plasmid elimination; 2-Keto-L-gulonic acid
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Funding
- Major State Basic Research Development Program of China (973 Program) [2012CB720806]
- National High Technology Research and Development Program of China (863 Program) [2012AA022103]
- Program for New Century Excellent Talents in University [NCET-12-0876]
- Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD) [2011046]
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In the two-step vitamin C fermentation process, the precursor 2-keto-L-gulonic acid (2-KLG) was synthesized using a mixed culture of Ketogulonicigenium vulgare WSH-001 and Bacillus megaterium WSH-002, which contained three plasmids, pBME1, pBME2 and pBME3. The cell growth of B. megaterium was not affected by the elimination of these plasmids. However, elimination of pBME2 and pBME3 significantly affected L-sorbose uptake and 2-KLG production. Sequence analysis of the plasmids showed that many of the pBME2 and pBME3 genes were of unknown function or could not be assigned to a specific metabolic pathway. The current work showed that the indigenous plasmids pBME2 and pBME3 of B. megaterium WSH-002 involved in mutualism with K. vulgare WSH-001. The results provided a promising new route to further demonstrate the mutualism process between the two bacteria. (C) 2013 Published by Elsevier Inc.
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