Review
Cell Biology
Mohamad Mahjoor, Arshia Fakouri, Simin Farokhi, Hojjatollah Nazari, Hamed Afkhami, Fatemeh Heidari
Summary: The skin is essential for the innate and adaptive immune systems, but skin lesions can have undesirable consequences. Mesenchymal stromal cells (MSCs) are being increasingly used for skin wound treatment due to their advantages in promoting healing. The oxygen concentration has an impact on MSC biology, and expanding MSCs in a hypoxic environment can enhance their effectiveness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Limberg Jaldin-Crespo, Nataly Silva, Jessica Martinez
Summary: This article summarizes the latest therapies for wound healing based on bionanomaterials assisted by honey and propolis, emphasizing their anti-inflammatory, antimicrobial, antioxidant, collagen synthesis stimulation, cell proliferation, and angiogenic effects. Nanomaterials enable targeted and continuous delivery at the site of the wound, avoiding systemic adverse effects.
Article
Biochemistry & Molecular Biology
Judith C. J. Holzer-Geissler, Simon Schwingenschuh, Martin Zacharias, Johanna Einsiedler, Sonja Kainz, Peter Reisenegger, Christian Holecek, Elisabeth Hofmann, Barbara Wolff-Winiski, Hermann Fahrngruber, Thomas Birngruber, Lars-Peter Kamolz, Petra Kotzbeck
Summary: The treatment of chronic wounds poses a challenge for modern medicine due to the complex pathophysiology involved. Previous wound models used for studying chronic wounds lack translatability to patients and often overlook the impact of exaggerated inflammation. This study aims to investigate the role of prolonged inflammation in a porcine wound model using the TLR7 and TLR8 agonist resiquimod. The results demonstrate that prolonged inflammation delays wound healing, a common observation in chronic wounds. This model highlights the importance of inflammation in wound healing and provides insights into the progression of chronic wounds.
Article
Engineering, Biomedical
Que Bai, Caiyun Zheng, Na Sun, Wenting Chen, Qian Gao, Jinxi Liu, Fangfang Hu, Tong Zhou, Yanni Zhang, Tingli Lu
Summary: This study presents an oxygen-releasing hydrogel that promotes cell survival, migration, and tube formation under hypoxic conditions, and facilitates tissue repair by reducing inflammation, increasing angiogenesis, and collagen deposition.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
M. C. Linju, M. R. Rekha
Summary: The present study developed a L-proline conjugated chitosan scaffold for wound healing. The scaffold was characterized by various tests and showed no cytotoxicity. In vitro experiments demonstrated that the modified scaffold promoted wound closure and collagen deposition. These findings suggest that the L-proline conjugated scaffold has potential as a wound dressing to improve wound healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Polymer Science
Siti Nor Najihah Yasin, Zulfahmi Said, Nadia Halib, Zulaiha A. Rahman, Noor Izzati Mokhzani
Summary: Excellent wound dressings should have crucial components such as high porosity, non-toxicity, high water absorption, and the ability to maintain a moist environment and promote wound healing. However, current wound dressings have limitations in antibacterial, anti-inflammatory, and antioxidant activity, frequent dressing changes, low biodegradability, and poor mechanical properties. Hydrogels, including natural, synthetic, or hybrid polymers, have shown potential as wound dressings by providing a moist environment, a protective barrier against infections, and pain relief. Honey-based hydrogel dressing, with its anti-inflammatory, antibacterial, and antioxidant properties, exhibits excellent characteristics including biodegradability, biocompatibility, cell proliferation, inhibition of bacterial growth, and accelerated wound healing. This review aims to showcase the potential of honey-based hydrogel dressings in wound healing applications and complement existing studies on their implementation.
Article
Plant Sciences
Parimah Ebadi, Mehdi Fazeli
Summary: The study found that Iranian propolis and honey had effects on HDF cell migration, proliferation, and viability in a dose-dependent manner, but no synergistic effects were observed between the two. The optimal concentrations for these effects were observed at 100 μg/ml and 200 μg/ml.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Polymer Science
Emine Salva, Ahmet Enes Akdag, Saadet Alan, Sema Arisoy, Fatma Julide Akbuga
Summary: The 3D polymeric network structure of chitosan/hyaluronic acid/honey hydrogels, which imitates the extracellular matrix, promotes cell growth and differentiation and exhibits excellent wound-healing abilities. The hydrogels were characterized and their properties, such as water content, water uptake capacities, and porosity, were investigated. The addition of honey altered the interaction between chitosan and hyaluronic acid, resulting in changes in the hydrogel's properties. The honey-containing hydrogels showed decreased porosity and slightly decreased water-uptake capacity, but increased cell adhesion and proliferation. In vivo studies demonstrated that honey addition accelerated wound healing. These findings highlight the potential of honey-loaded chitosan-hyaluronic acid hydrogels in wound healing applications.
Article
Pharmacology & Pharmacy
Vandiara Martins Moreira, Joandra Maisa da Silva Leite, Kaline de Araujo Medeiros, Karoll Moangella Andrade de Assis, Joyce Cordeiro Borges, Lucas Matheus Barreto Santana, Livia Maria Coelho de Carvalho Moreira, Larissa Pereira Alves, Tharcia Kiara Beserra de Oliveira, Joao Walter de Souza da Silveira, Dayanne Tomaz Casimiro da Silva, Bolivar Ponciano Goulart de Lima Damasceno
Summary: The study developed chitosan films associated with pentoxifylline for healing wounds. The films were prepared at two concentrations and evaluated for their structural characteristics, release properties, and wound healing effects in mice. Higher concentration of pentoxifylline in the films showed faster wound reduction, indicating the effectiveness of the combination of chitosan and pentoxifylline in promoting wound healing.
Review
Dermatology
Andrzej Hecker, Marlies Schellnegger, Elisabeth Hofmann, Hanna Luze, Sebastian Philipp Nischwitz, Lars-Peter Kamolz, Petra Kotzbeck
Summary: Resveratrol is a powerful antioxidant that may aid in wound healing and prevent skin aging. Current research suggests that resveratrol could be a feasible treatment approach, but further studies are needed to confirm its effects.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Filip Humenik, Marcela Maloveska, Nikola Hudakova, Patricia Petrouskova, Zuzana Sufliarska, L'ubica Hornakova, Alexandra Valencakova, Martin Kozar, Barbora Siskova, Dagmar Mudronova, Martin Bartkovsky, Dasa Cizkova
Summary: The aim of this study was to investigate the beneficial treatment effect of canine amniotic membrane-derived mesenchymal stem cell products (AM-MSC) on complicated wound healing in dogs. Characterization of AM-MSCs showed their distinctive features and potential for differentiation. In vitro studies demonstrated a significant decrease in scratch area on skin fibroblast cultures treated with canine amniotic mesenchymal stem cell conditioned media (AMMSC-CM). In the experimental study, the combination therapy of antibiotics, NSAIDs, and local AMMSC-CM application resulted in a substantial reduction in wound surface area compared to conventional therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Joel Yupanqui Mieles, Cian Vyas, Enes Aslan, Gavin Humphreys, Carl Diver, Paulo Bartolo
Summary: Honey is experiencing a resurgence in evaluation for antimicrobial and wound healing applications due to its ability to inhibit various bacteria, fungi, and viruses, as well as promote wound healing. Its activity is attributed to the enzymatic generation of hydrogen peroxide and other non-peroxide factors. The development of tissue engineering approaches has been driven by the difficulty of delivering honey's active ingredients to the wound site.
Article
Biochemistry & Molecular Biology
Qi Zhang, Miao Zhang, Teng Wang, Xiangyan Chen, Quancai Li, Xia Zhao
Summary: Maintaining a moist and sterile environment is beneficial for wound healing. A novel natural multifunctional hydrogel, combining Aloe polysaccharide (AP) and honey, was prepared for infected wound healing. The hydrogel exhibited good water retention capacity, antibacterial activity, and accelerated the healing of infected wounds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Afnan Harb, Mahmoud H. Elbatreek, Ahmed Elshahat, Nadia El-Akabawy, Waleed Barakat, Nesreen MIM. Elkomy
Summary: This study reveals the potential of alagebrium in the treatment of diabetic foot ulcer (DFU), as it promotes wound healing, improves sensory function and gait, reduces inflammation, and enhances angiogenesis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hairul-Islam Mohamed Ibrahim, Muthukumar Thangavelu, Ashraf Khalifa
Summary: In this study, collagen hydrolysates modified with honey-propolis wax (HPW) were tested on wound healing in a mouse model. Results showed that the CHDs-HPW mixture significantly enhanced wound healing and reduced inflammation, indicating its potential as a therapeutic approach for damaged human skin.
Article
Genetics & Heredity
Sanmitra Basu, Amrita Chaudhary, Pramita Chowdhury, Deepmala Karmakar, Keya Basu, Dilip Karmakar, Jyotirmoy Chatterjee, Sanghamitra Sengupta
Article
Biotechnology & Applied Microbiology
Tila Khan, Ranjan Saurav Das, Bikas K. Arya, Amrita Chaudhary, Jyotirmoy Chatterjee, Sangeeta Das Bhattacharya
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2020)
Review
Chemistry, Medicinal
Abhishek Gour, Diksha Manhas, Swarnendu Bag, Bapi Gorain, Utpal Nandi
Summary: The emergence of SARS-CoV-2 and COVID-19 has caused worldwide panic since December 2019, prompting the search for novel therapeutics. Plant natural products, especially flavonoids, have shown potential in immunomodulation to combat the cytokine release storm during COVID-19 infection. Research on these natural products may lead to the development of effective and safe management strategies for SARS-CoV-2.
PHYTOTHERAPY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Debabrata Dutta, Shakilur Rahman, Gourab Bhattacharje, Swarnendu Bag, Bidhan Chandra Sing, Jyotirmoy Chatterjee, Amit Basak, Amit Kumar Das
Summary: A label-free method using mass spectrometry was developed to identify and map hydroxylated proteins in human plasma, revealing 11 hydroxylated proteins, with proline hydroxylation in IgA1 causing structural changes validated by molecular dynamics analysis. This study provides a comprehensive understanding of Hyp PTMs in the plasma proteome and indicates future prospects for distinguishing normal and diseased proteomes.
Article
Chemistry, Multidisciplinary
Shipra Bhatt, Ankita Sharma, Ashish Dogra, Priyanka Sharma, Amit Kumar, Pankul Kotwal, Swarnendu Bag, Prashant Misra, Gurdarshan Singh, Ajay Kumar, Payare Lal Sangwan, Utpal Nandi
Summary: The study aimed to explore the protective effect of glabridin on PCM-induced liver injury and found that glabridin significantly improved biochemical parameters and oxidative stress markers, suppressed CYP2E1 expression, and reduced NF-kappa B activation.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Medicine, Research & Experimental
Ashish Dogra, Divya Gupta, Swarnendu Bag, Irfan Ahmed, Shipra Bhatt, Ekta Nehra, Shakti Dhiman, Amit Kumar, Gurdarshan Singh, Sheikh Tasduq Abdullah, Payare Lal Sangwan, Utpal Nandi
Summary: The study demonstrated that glabridin effectively prevented methotrexate-induced liver injury by modulating oxidative stress, inflammation, and apoptosis.
Article
Integrative & Complementary Medicine
Debalina Bose, Amrita Chaudhary, Manchikanti Padmavati, Jyotirmoy Chatterjee, Rintu Banerjee
Summary: This study observed the cytotoxic activity of honey from Litchi chinensis and purified honey protein (PHP) on HeLa cells. Both honey and PHP significantly reduced cancer cell viability and induced apoptosis in a dose-dependent manner. The IC(50) of honey and PHP were found to be 3.5 mg/mL and 4.13 ng/mL, respectively. PHP intervention resulted in more apoptosis compared to honey treatment. These findings provide scientific rationale for the development of effective anti-proliferative agents and endorse the use of lychee honey and PHP as herbal medicines.
JOURNAL OF HERBAL MEDICINE
(2022)
Article
Anatomy & Morphology
Arpan Haldar, Manisha RajanGaikwad, Apurba Patra, Soumya C. Bhattacharya
Summary: This study examined the detailed histogenesis and development of the fetal liver using cytological, immunohistochemical, and ultrastructural methods. It found that duct patterns and hematopoietic patterns develop at all stages of fetal development. The organizational changes in lobular pattern, duct pattern, and microstructure of the liver during fetogenesis are crucial for achieving adult morphology in the future.
ANATOMY & CELL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ashish Dogra, Dilpreet Kour, Abhishek Gour, Mahir Bhardwaj, Swarnendu Bag, Shakti Kumar Dhiman, Ajay Kumar, Gurdarshan Singh, Utpal Nandi
Summary: Rutin can protect against diclofenac-induced cardiac injury by reducing oxidative stress, inhibiting inflammation, and reducing apoptosis.
DRUG AND CHEMICAL TOXICOLOGY
(2023)
Article
Cell Biology
Swarnendu Bag, Janina Oetjen, Soni Shaikh, Amrita Chaudhary, Pattatheyil Arun, Geetashree Mukherjee
Summary: MALDI imaging and immunohistochemistry were used to characterize the spatial biology of two primary oral tumours, one from a patient with early recurrence (Tumour R), and the other from a patient with no recurrence after 2 years (Tumour NR). Tumour R showed increased purine nucleotide metabolism in different regions and adenosine-mediated suppression of immune cells compared to Tumour NR. Differentially expressed markers in Tumour R included CD33, CD163, TGF-beta, COX2, PD-L1, CD8, and CD20. These findings suggest that altered tumour metabolomics and immune microenvironment could potentially serve as markers for recurrence.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Vartika Lohani, A. R. Akhiya, Soumen Kundu, M. D. Quasid Akhter, Swarnendu Bag
Summary: Nowadays, the concept of single-cell proteomics (SCP) has gained attention, especially in clinical research, due to its ability to identify specific proteomic signatures in diseased cells. This information is crucial for understanding the progression of diseases like cancer, diabetes, and Alzheimer's. Conventional destructive proteomics provides only an average idea of protein expression, whereas SCP along with spatial attributes allows for the study of heterogeneous protein functions. Various techniques, including fluorescence-activated cell sorting, mass spectrometry-based proteomics tools, are used to isolate and study single cells. This review primarily focuses on the use of mass spectrometry-based approaches in single-cell proteomics.
Article
Oncology
Ankita Chatterjee, Amrita Chaudhary, Arnab Ghosh, Pattatheyil Arun, Geetashree Mukherjee, Indu Arun, Arindam Maitra, Nidhan Biswas, Partha P. Majumder
Summary: Deep immune profiling of gingivobuccal oral cancer patients revealed that patients with poor prognosis had restricted immune cell infiltration in tumors but not in margins. Reduced expression of 8 immune-related genes in tumors strongly predicted better prognosis. Targeting CD73 immune-checkpoint inhibition may improve clinical outcome.
Article
Medicine, General & Internal
Arpan Haldar, Manisha Gaikwad, Apurba Patra, Soumya Chakraborty
Summary: The study investigated the histogenesis patterns of the spleen in relation to gestational age using various microscopy and immunohistochemistry techniques. The developmental changes in the spleen during fetogenesis were found to be crucial for adult morphology, with deviations from normalcy potentially leading to structural and functional abnormalities later in life.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Anurup Mukhopadhyay, Ankita Das, Suranjana Mukherjee, Monika Rajput, Ayan Gope, Amrita Chaudhary, Kabita Choudhury, Ananya Barui, Jyotirmoy Chatterjee, Rabibrata Mukherjee
Summary: The hierarchically patterned porous honey-incorporated silk fibroin scaffolds fabricated using soft lithography and freeze-drying techniques show improved biocompatibility and proliferation of adipose-derived mesenchymal stem cells (ADMSCs). The honey-release scaffolds exhibit sustained antibacterial efficacy against MRSA and regulate ADMSC behavior by altering their cytoskeletal rearrangement for increased cell spreading and sheet formation. The synergistic effect of biochemical (honey) and biophysical (patterns) cues on ADMSCs highlights the potential of these scaffolds for regenerative healing implant applications.
ACS APPLIED BIO MATERIALS
(2021)