Article
Plant Sciences
Enuo Liu, Hiroshi Tsuboi, Shuuji Ikegami, Tomonori Kamiyama, Yukio Asami, Luyi Ye, Munehiro Oda, Zai-Si Ji
Summary: The main components of Nelumbo nucifera leaf extract (NnEx), quercetin-3-O-ss-glucuronide (Q3GA) and quercetin, were found to promote lipid metabolism by inhibiting the cAMP pathway. Ingestion of NnEx may provide a novel treatment for obesity and type II diabetes by modulating fat and sugar absorption.
PLANT FOODS FOR HUMAN NUTRITION
(2021)
Review
Chemistry, Applied
Xu Zhao, Ran Zhao, Xiaojing Yang, Lihui Sun, Yongming Bao, Yuan Shuai Liu, Andreas Blennow, Xingxun Liu
Summary: This review focuses on the biosynthesis and types of flavonoids, alkaloids, and lipids in lotus plants, as well as their physiological functions and applications in traditional medicine and scientific research. Future studies should prioritize the standardization of lotus extracts and their pharmacological mechanisms as drugs or functional foods. This review is important for lotus-based food processing and application.
Article
Multidisciplinary Sciences
Hui Li, Xingyu Yang, Yue Zhang, Zhiyan Gao, Yuting Liang, Jinming Chen, Tao Shi
Summary: The Nelumbo Genome Database (NGD) is a comprehensive database that provides updated lotus genome information, gene expression and coexpression network data, genetic variants, key traits, and various tools for sequence analysis and gene searching. This valuable genomic resource will facilitate future studies on lotus population genetics and molecular breeding.
Article
Biology
Mingyue Luo, Hui Jia, Qijia Li, Xiangning Meng, David K. Ferguson, Ping Liu, Zhuochen Han, Junjie Wang, Cheng Quan
Summary: The new species of lotus reported in this paper is the first fossil record of lotus in Northwest China, providing valuable information on the paleoenvironment of the Qaidam Basin. The findings suggest that during the late Middle Miocene, a large freshwater lake surrounded by temperate forests and grassland existed in this area.
Article
Plant Sciences
Mikhail S. Romanov, Alexey V. F. C. H. Bobrov, Ekaterina S. Romanova, Nikita S. Zdravchev, Alexey N. Sorokin
Summary: This study investigated the fruitlet development in Nelumbonaceae, revealing its unique fruit structure and histology. It also proposed additional autapomorphies for Nelumbonaceae and demonstrated the relationship between Nelumbonaceae, Platanaceae, and some Proteaceae in Proteales.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Jiao Deng, Juanjuan Li, Mengyue Su, Zhongyuan Lin, Lei Chen, Pingfang Yang
Summary: Lotus is an important aquatic ornamental plant with key horticultural traits, and anthocyanins play important roles in determining the red color of lotus flowers. The NnTT8 gene in lotus is involved in regulating anthocyanin biosynthesis, which could be crucial in breeding high anthocyanin content lotus varieties for potential lotus flower beverages.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Jiraporn Laoung-on, Churdsak Jaikang, Kanokporn Saenphet, Paiwan Sudwan
Summary: The study found that the aqueous extract of white lotus petals has higher antioxidant activity and promotes sperm viability, reducing oxidative stress and improving sperm vitality.
Article
Plant Sciences
Dingding Cao, Zhongyuan Lin, Longyu Huang, Rebecca Njeri Damaris, Ming Li, Pingfang Yang
Summary: The study demonstrates that the CONSTANS-LIKE gene family, particularly COL5, plays a role in regulating potato tuber expansion and is positively associated with the enlargement of lotus rhizome. Further analysis suggests that this gene may affect the expression of genes in CO-FT and GA signaling pathways, leading to changes in tuber size and starch content.
Article
Biochemistry & Molecular Biology
Gui-Zhen Chen, Jie Huang, Xiao-Qin Zhou, Yang Hao, Jin-Liao Chen, Yu-Zhen Zhou, Sagheer Ahmad, Siren Lan, Zhong-Jian Liu, Dong-Hui Peng
Summary: This study provides a comprehensive overview of the GRF transcription factor family in Nelumbo nucifera. Through phylogenetic analysis, gene structure analysis, motif analysis, and analysis of cis-acting regulatory elements, eight GRF genes were identified in the Nelumbo nucifera genome. The expression pattern of the GRF genes was analyzed based on transcriptomic data and real-time reverse transcription-quantitative PCR (qRT-PCR). The study also predicted the 3D structure of NuGRF proteins and determined the subcellular localization of GRF1 in tobacco leaves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Anne-Lise Marie, Somak Ray, Shulin Lu, Jennifer Jones, Ionita Ghiran, Alexander R. Ivanov
Summary: A highly sensitive method for profiling N-glycans released from proteins using CZE-ESI-MS was developed and applied to glycan analysis of plasma and blood-derived isolates. The technique significantly improved detection sensitivity and resolution, allowing for qualitative and quantitative analysis of N-glycans in blood samples with unmatched depth compared to previous methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Peng Wu, Ailian Liu, Yongyan Zhang, Kai Feng, Shuping Zhao, Liangjun Li
Summary: The study revealed that ABA induces upregulation of NnABI4 expression, which in turn promotes NnSS1 expression, enhancing starch accumulation in lotus rhizomes. This provides a crucial theoretical basis for studying the molecular mechanism of ABA regulation of starch synthesis in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Rungnapa Pankla Sranujit, Chanai Noysang, Patcharaporn Tippayawat, Nateelak Kooltheat, Thitiya Luetragoon, Kanchana Usuwanthim
Summary: The study identified phytochemicals in lotus flower and examined the antioxidant and immunomodulatory properties of ethyl acetate (EA) and ethyl alcohol (ET) lotus petal extracts. The findings revealed that the extracts possess high antioxidant capability and can beneficially impact individuals with compromised immune functions by suppressing TNF-alpha secretion and NF-kappa B-dependent inflammatory response.
Article
Plant Sciences
Sung Ho Moon, Euihyun Kim, Hye-In Kim, Soo-Yun Kim, Hyo-Hyun Seo, Jeong Hun Lee, Min-Sup Lee, Seung-Ki Lee, Sang Hyun Moh, Seunghee Bae
Summary: The study found that the extract derived from lotus callus in the Haman region not only has anti-inflammatory, antioxidant, and soothing properties, but also plays an important role in skin whitening. Clinical trials have confirmed the significant whitening effect of cosmetics containing this extract.
Article
Agriculture, Multidisciplinary
Zebron Nchimunya Tembo, Seker Fatma Aygun
Summary: Teas and traditional herbs are commonly used for health purposes, but they may contain carcinogenic contaminants. This study developed a quick method using capillary electrophoresis for simultaneous determination of nitrate, nitrite, and bromate ions in selected plants and tea samples. Optimal conditions were determined, and the method was found to be quick and efficient with detection achievable within 6 minutes.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Plant Sciences
Hieu Nguyen Ngoc, Mostafa Ahlou, Marlies Stonig, Duc Trong Nghiem, Le Thien Kim, Johanna M. Gostner, Hermann Stuppner, Markus Ganzera
JOURNAL OF NATURAL PRODUCTS
(2019)
Article
Chemistry, Medicinal
Stefanie Hofer, Anja Hartmann, Maria Orfanoudaki, Hieu Nguyen Ngoc, Markus Nagl, Ulf Karsten, Svenja Heesch, Markus Ganzera
Article
Chemistry, Medicinal
Maria Orfanoudaki, Anja Hartmann, Mostafa Alilou, Thomas Gelbrich, Patricia Planchenault, Severine Derbre, Andreas Schinkovitz, Pascal Richomme, Andreas Hensel, Markus Ganzera
Article
Biochemistry & Molecular Biology
Stefanie Hofer, Simon Geisler, Rebecca Lisandrelli, Hieu Nguyen Ngoc, Markus Ganzera, Harald Schennach, Dietmar Fuchs, Julian E. Fuchs, M. Johanna Gostner, Katharina Kurz
Article
Biochemistry & Molecular Biology
Maria Orfanoudaki, Anja Hartmann, Mitsunobu Kamiya, John West, Markus Ganzera
Article
Biochemistry & Molecular Biology
Maria Orfanoudaki, Anja Hartmann, Mostafa Alilou, Naida Mehic, Marcel Kwiatkowski, Karin Joehrer, Hieu Nguyen Ngoc, Andreas Hensel, Richard Greil, Markus Ganzera
Summary: The study focused on investigating the chemical compositions of two Mediterranean demosponges, isolating 20 pure secondary metabolites with interesting biological activities, including 5 new natural products. These compounds were found to have diverse chemical scaffolds and exhibited cytotoxic properties in multiple cell lines, with one compound showing particularly promising activities.
Article
Chemistry, Analytical
Michael Zwerger, Markus Ganzera
Summary: Boswellic acids, bioactive constituents in Indian frankincense, were successfully separated and quantified in B. serrata extracts using Supercritical Fluid Chromatography for the first time. The method showed excellent performance in terms of accuracy, precision, and sensitivity, with potential for application in analyzing commercial products with variable concentrations of boswellic acids.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Medicinal
Maria Orfanoudaki, Anja Hartmann, Julia Mayr, Felix L. Figueroa, Julia Vega, John West, Ricardo Bermejo, Christine Maggs, Markus Ganzera
Summary: This study validates a HPLC-DAD method for the determination of MAAs in the red alga Bostrychia scorpioides, discovering minor differences in the MAA composition among samples collected from four European countries. The study confirms the consistency in MAA composition of the investigated B. scorpioides samples, in line with the reported monophyly of this specific Bostrychia species.
Article
Chemistry, Analytical
Fabian Hammerle, Michael Zwerger, Anja Hoeck, Markus Ganzera, Ursula Peintner, Bianka Siewert
Summary: Recently, it has been discovered that certain fungal pigments have significant photoactivity, making them promising candidates for treating microbial infections or malignant diseases. In this study, a separation method for fungal anthraquinones was developed using centrifugal partition chromatography. The method was successfully applied to purify multiple pigments from fungal extracts.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Medicinal
Michael Zwerger, Stefan Schwaiger, Markus Ganzera
Summary: A novel approach for the isolation of two specific secondary metabolites, mycosporine-like amino acids (MAAs), from marine rhodophyta was developed. The method combined fast centrifugal partition chromatography and solid phase extraction, enabling efficient purification in a shorter time frame compared to established protocols. The isolated MAAs were highly pure, as confirmed by various analyses.
Review
Chemistry, Analytical
Markus Ganzera, Michael Zwerger
Summary: SFC has been proven to be an excellent analytical technique for investigating a wide range of compounds, including those with different polarities, acidities/alkalinity, and require chiral separations. Its flexibility allows compatibility with different stationary phase chemistries and detection modes, while online coupling to SFE is also a new technical option.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Michael Zwerger, Markus Ganzera
Summary: In this study, a new UHPLC method was developed for the determination of eleven MAAs in red algae. The results showed significant variations in MAA profiles and content among different algal species.
Article
Biotechnology & Applied Microbiology
Michael J. Zwerger, Fabian Hammerle, Bianka Siewert, Markus Ganzera
Summary: Marine red algae are a valuable source for natural sunscreens and antioxidants due to their synthesis of unique secondary metabolites to protect themselves from high levels of UV radiation. This study used HR-MS/MS metabolomics to detect and classify algal natural products, with a focus on mycosporine-like amino acids (MAAs). By employing UHPLC hyphenated to orbitrap HRMS2, a feature-based molecular network (FBMN) was created and matched against an in-house and open source library on the GNPS platform. In combination with computational annotation tools and published fragmentation patterns, known compounds were confirmed and structures for novel substances were proposed.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Chemistry, Analytical
Michael Zwerger, Judith Zelger, Markus Ganzera
Summary: The neophyte plant species S. inaequidens from South Africa has spread widely throughout Europe and the world. All species in this genus contain toxic pyrrolizidine alkaloids (PAs), which pose a potential health hazard to humans and livestock. Efficient and straightforward assays for their qualitative and quantitative analysis are in high demand due to their presence in the food chain and contaminants in herbal crops and phytopharmaceutical formulations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Michael Zwerger, Sarah Contratti, Valentina Mariano, Markus Ganzera
Summary: In recent years, liquid chromatography (LC) and gas chromatography (GC) have been the main methods for analyzing natural products, but alternative methods such as capillary electrophoresis (CE) and supercritical fluid chromatography (SFC) with orthogonal selectivity have also been increasingly used. In this study, CE and SFC methods were developed and compared for analyzing bioactive compounds in apple leaves. Both methods were able to separate the target compounds efficiently within a short time and meet the requirements for environmentally friendly technologies. Analysis of different apple samples using the optimized methods revealed significant variations in the DHC profile, with overall DHC concentrations ranging from 5.47% to 17.24%.
