Article
Cell & Tissue Engineering
Stefan Balko, Evan Kerr, Ed Buchel, Sarvesh Logsetti, Afshin Raouf
Summary: Stromal vascular fraction (SVF) cells and keratinocytes engage in a paracrine crosstalk during wound closure, resulting in a new cytokine profile that can be used to accelerate wound healing and initiate re-epithelialization.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Justyna Mazurkiewicz, Aleksandra Simiczyjew, Ewelina Dratkiewicz, Magdalena Kot, Katarzyna Pietraszek-Gremplewicz, Dominika Wilk, Marcin Zietek, Rafal Matkowski, Dorota Nowak
Summary: This study provides a more in-depth understanding of the role of keratinocytes in the melanoma microenvironment, highlighting their unique secretome and mediators of invasive abilities, which could be utilized by cancer cells to support their invasion of surrounding tissues.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Physiology
Peng Zhou, Vanessa Chrepa, Ioannis Karoussis, Michael A. Pikos, Georgios A. Kotsakis
Summary: This study found that an herbal extract compound-based oral rinse was cytocompatible to cells critical to oral wound healing and promoted fibroblast migration and differentiation, contrary to existing antiseptic rinses that lack selective cytotoxicity.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Claudia Vater, Leonie Bosch, Alexandra Mitter, Thomas Goels, Saskia Seiser, Elke Heiss, Adelheid Elbe-Buerger, Michael Wirth, Claudia Valenta, Victoria Klang
Summary: Previous studies have shown that lecithin-based nanoemulsions are skin-friendly drug carrier systems and may also be suitable for wound healing. Incorporation of betulin-enriched extract from birch bark and purified spruce balm into NEs positively influence cell viability, proliferation, and wound closure. NEs with these natural wound healing agents displayed better wound closure rates compared to control formulations in in vitro studies.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Chemistry, Analytical
Ehsan Yazdanpanah Moghadam, Nahum Sonenberg, Muthukumaran Packirisamy
Summary: This article investigates the migration behavior of microglia cells on different substrates using a microfluidic wound-healing chip. The results show that certain substrate types can promote cell migration, while others may have inhibitory effects.
Article
Biochemistry & Molecular Biology
Sun-Hye Choi, Kyung-Jong Won, Rami Lee, Han-Sung Cho, Sung-Hee Hwang, Seung-Yeol Nah
Summary: This study demonstrates that gintonin promotes wound healing by enhancing migration and proliferation of skin cells, as well as increasing vascular endothelial growth factor release, possibly through activation of LPA receptors and downstream signaling pathways involving VEGF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Edward J. Calabrese, Gaurav Dhawan, Rachna Kapoor, Evgenios Agathokleous, Vittorio Calabrese
Summary: This article investigates the commonly observed hormetic dose responses in dermal wound healing, specifically focusing on cell viability, proliferation, and migration of human keratinocytes in in vitro studies. Hormetic responses can be induced by a wide range of substances, including endogenous agents, drugs, and plant derived extracts. The findings suggest that hormesis plays a fundamental role in wound healing and has important implications for agent screening, evaluation, and clinical strategies.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Cell Biology
Lei Qiang, Seungwon Yang, Yan-Hong Cui, Yu-Ying He
Summary: The study found that inhibiting epidermal cell autophagy impairs wound healing in mice, affecting processes such as keratinocyte proliferation, immune cell infiltration, and dermal tissue formation. TNF induces CCL2 transcription through the autophagy-AMPK-BRAF-MAPK1/3/ERK-AP1 pathway, influencing keratinocyte migration and proliferation, and activating dermal fibroblasts.
Article
Pharmacology & Pharmacy
Lucelia Albarello Balestrin, Taina Kreutz, Flavia Nathiely Silveira Fachel, Juliana Bidone, Nicolly Espindola Gelsleichter, Leticia Scherer Koester, Valquiria Linck Bassani, Elizandra Braganhol, Cristiana Lima Dora, Helder Ferreira Teixeira
Summary: The study investigated the effects of ASE-loaded nanoemulsions (NEASE) on HaCaT cells, showing positive impacts on cell viability, death by necrosis, migration, and irritant potential. NEASE was found to increase cell migration at low concentrations.
Article
Pharmacology & Pharmacy
Myla Lobo de Souza, Douglas Dourado, Ivon Pinheiro Lobo, Vinicius Couto Pires, Sara Nunes de Oliveira Araujo, Juliana de Souza Reboucas, Ana Maria Costa, Caio Pinho Fernandes, Natalia Machado Tavares, Neila de Paula Pereira, Fabio Rocha Formiga
Summary: This study evaluated the physicochemical properties and cellular effects of seed oils from wild Passiflora species. The results showed that these oils had good cytocompatibility and could stimulate the proliferation of keratinocytes. Incorporating them into nanoemulsions for use in dermatological products can provide superior outcomes.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Fariba Saadati, Walison Augusto da Silva Brito, Steffen Emmert, Sander Bekeschus
Summary: Research suggests that nano- and micro-plastic particles (NMPPs) may pose a potential threat to human health. In this study, non-malignant HaCaT keratinocytes were exposed to different types, concentrations, and sizes of polystyrene (PS) and poly methyl methacrylate (PMMA) particles to determine their ability to induce micronuclei (MN) formation. The results showed that most non-charged polymer particles were neither toxic nor genotoxic, while aminated particles were toxic but not genotoxic.
Article
Endocrinology & Metabolism
Peng Zhou, Haijun Feng, Wenhui Qin, Qin Li
Summary: Impaired diabetic wound healing is a significant issue in diabetic complications. Hyperkeratosis may affect the speed of wound healing. Increased KRT17 expression under high glucose stimulates keratinocyte proliferation and migration through the c-MYB/PI3K-AKT pathway, leading to hyperkeratosis and delayed wound healing.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Medicine, Research & Experimental
Chi-Cheng Lu, Jai-Sing Yang, Yu-Jen Chiu, Fuu-Jen Tsai, Yuan-Man Hsu, Mei-Chin Yin, Yu-Ning Juan, Tsung-Jung Ho, Hao-Ping Chen
Summary: Dracorhodin perchlorate promotes wound healing in human keratinocytes by enhancing cell migration and activating specific signaling pathways.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Genetics & Heredity
Khrystyna Sych, Simon P. Nold, Josef Pfeilschifter, Rajkumar Vutukuri, Jana Meisterknecht, Ilka Wittig, Stefan Frank, Itamar Goren
Summary: In injured skin, intact insulin signaling and glucose uptake are crucial for proper wound closure. mRNA and protein analysis revealed that the expression of genes involved in glycolysis, oxidative phosphorylation, pentose phosphate shunt, and glutamine anaplerosis are boosted during wound healing. Specifically, PKM2 plays a significant role in facilitating VEGF induction in keratinocytes during wound repair.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Pharmacology & Pharmacy
S. N. Yuksel, M. Dikmen, Z. Canturk
Summary: The study found that helenalin promotes cell proliferation, wound healing, and anti-inflammatory effects on human keratinocyte cells. Under inflammatory conditions, helenalin at concentrations of 0.02 and 0.2 μM increased keratinocyte proliferation and induced more wound healing and anti-inflammatory activity.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Bruno Burlando, Laura Cornara, Raffaella Boggia
Summary: This review focuses on three species of berries, including the high-latitude cloudberry, arctic bramble, and high-altitude yellow raspberry. These berries are rich in ellagitannins (ETs) and other phenolic compounds, which make them potential nutraceuticals with antioxidant and bioactive properties. The health benefits of ETs include anti-inflammatory, antiviral, anti-bacterial, and anticancer actions. The review covers ethnobotanical, pharmaceutical, medicinal, and nutritional knowledge about these berries, revealing a wide range of potential uses in health care.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Food Science & Technology
Antonella Rosa, Mariella Nieddu, Federica Pollastro, Cristina Piras
Summary: The fatty acid (FA) composition of cell membranes is a metabolic biomarker. This study validates the use of cell FA ratios as alternative metabolic markers. The results show that FA ratios can serve as a cell-specific fingerprint with reproducibility across intra-laboratory conditions.
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mariella Nieddu, Federica Pollastro, Paola Caria, Stefano Salamone, Antonella Rosa
Summary: This study evaluated the impact of the flavone xanthomicrol on lipid metabolism in cancer cells and investigated the changes in cell growth, morphology, and apoptosis induced by treatment. Xanthomicrol significantly reduced cell viability and modulated the lipid profile in cancer cells. It also induced noticeable changes in cell morphology, apoptosis, and cell cycle arrest at the G2/M phase.
Article
Biochemistry & Molecular Biology
Carlotta Bollati, Stefania Marzorati, Lorenzo Cecchi, Martina Bartolomei, Jianqiang Li, Maria Bellumori, Lorenza d'Adduzio, Luisella Verotta, Laura Piazza, Anna Arnoldi, Nadia Mulinacci, Carmen Lammi
Summary: To improve the management of the production chain of PGI Mantua pears, fruits with diseases or defects were used to produce dried rounds of pears, extending the remaining life. The secondary and residual by-products of the pears were then utilized to extract valuable phytocompounds using traditional and green extraction methods. The extracted compounds showed antioxidant activity, suggesting their potential use in dietary supplements and functional foods.
Article
Plant Sciences
Ivan Anchesi, Federica Betto, Luigi Chiricosta, Agnese Gugliandolo, Federica Pollastro, Stefano Salamone, Emanuela Mazzon
Summary: In this study, NSC-34 cells were treated with CBG at a concentration of 10 μM, and the effects of CBG on these cells were evaluated using NGS technology. The analysis showed that CBG activates the WNT/planar cell polarity pathway and Ephrin-Eph signaling. The results indicated that CBG increases the expression of genes associated with cytoskeletal remodeling and axon guidance.
Article
Plant Sciences
Stefano Salamone, Nicola Aiello, Pietro Fusani, Antonella Rosa, Mariella Nieddu, Giovanni Appendino, Federica Pollastro
Summary: Musk yarrow, an endemic plant in the Central Alps, is used for flavoring alcoholic beverages. However, little is known about its chemical composition. To address this issue, a sample of musk yarrow from the Italian Eastern Alps was studied, and several compounds were identified as potential markers for validating the supply chain.
Article
Biochemistry & Molecular Biology
Ivan Anchesi, Giovanni Schepici, Luigi Chiricosta, Agnese Gugliandolo, Stefano Salamone, Diego Caprioglio, Federica Pollastro, Emanuela Mazzon
Summary: Cannabinoids, both natural and synthetic, possess various properties such as antidepressant, anxiolytic, anticonvulsant, and anti-psychotic effects. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (?(9)-THC) are extensively studied cannabinoids, while delta-8-tetrahydrocannabinol (?(8)-THC), an isomer of ?(9)-THC, has received less attention. This study aimed to investigate the impact of ?(8)-THC on gene expression related to synaptic pathways using next generation sequencing (NGS) in SH-SY5Y human neuroblastoma cells. The findings revealed that ?(8)-THC upregulated genes involved in the glutamatergic pathway and inhibited gene expression at cholinergic synapses, but had no effect on genes related to the GABAergic and dopaminergic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Antonella Smeriglio, Susanna Alloisio, Raffaella Barbieri, Mariarosaria Ingegneri, Paola Malaspina, Bruno Burlando, Laura Cornara, Domenico Trombetta
Summary: Citrus lumia Risso and Poit. 'Pyriformis' are horticultural varieties of Citrus lumia Risso. The fruit is very fragrant and pear-shaped, with a bitter juice, a floral flavor, and a very thick rind. The essential oil extracted from the rind has shown interesting antioxidant, anti-inflammatory, and neuroinhibitory effects, as well as acetylcholinesterase inhibitory activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Agnese Gugliandolo, Santino Blando, Stefano Salamone, Diego Caprioglio, Federica Pollastro, Emanuela Mazzon, Luigi Chiricosta
Summary: Alzheimer's disease is characterized by amyloid beta plaques and neurofibrillary tangles. This study evaluated the neuroprotective potential of Delta(8)-THC pretreatment in an in vitro model of AD and found that it restored cell viability and reduced ER stress and neuronal apoptosis. This suggests that Delta(8)-THC may be a new neuroprotective agent in AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Foteini D. Kalousi, Federica Pollastro, Aikaterini G. Karra, Ioannis Tsialtas, Achilleas Georgantopoulos, Stefano Salamone, Anna-Maria G. Psarra
Summary: Chios Mastiha, the resin of Pistacia lentiscus L. var. chia, is a natural chewing gum widely used in Mediterranean cuisine and traditional medicine. It is rich in triterpenes, which have anti-inflammatory activities similar to glucocorticoids. Different polarity fractions of Chios Mastiha have been found to exhibit anti-proliferative, anti-inflammatory, and apoptotic activities, and interfere with glucocorticoid receptor signaling.
Article
Plant Sciences
Martina Tamburello, Stefano Salamone, Lisa Anceschi, Paolo Governa, Virginia Brighenti, Alice Morellini, Giada Rossini, Fabrizio Manetti, Giorgio Gallinella, Federica Pollastro, Federica Pellati
Summary: In this study, the antiviral activity of cannabinoids isolated from Cannabis sativa L. against SARS-CoV-2 variants was evaluated. Cannabidiolic acid (CBDA) was identified as the most active compound, and its methyl ester derivative showed an even stronger antiviral effect. The stability of CBDA methyl ester was confirmed through analysis using UHPLC and HRMS, and its interaction with the virus spike protein was assessed in silico. These findings suggest that CBDA methyl ester could be a promising lead compound for the development of a new effective drug against COVID-19.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Antonella Smeriglio, Joseph Lionti, Mariarosaria Ingegneri, Bruno Burlando, Laura Cornara, Federica Grillo, Luca Mastracci, Domenico Trombetta
Summary: The study investigated the use of the diatom Phaeodactylum tricornutum Bohlin (Phaeodactylaceae) as a functional ingredient for nutraceutical and cosmeceutical applications. It was found that the extract from this diatom, which contains high levels of neutral lipids and xanthophylls, exhibited excellent antioxidant activity and photostability against UVA and UVB rays. In addition, it was also shown to effectively reduce oxidative and inflammatory stress markers induced by solar radiation, making it a potentially safe and effective ingredient in broad-spectrum sunscreens.
Article
Chemistry, Medicinal
Vittoria Borgonetti, Clarissa Caroli, Paolo Governa, Brighenti Virginia, Federica Pollastro, Silvia Franchini, Fabrizio Manetti, Francisco Les, Victor Lopez, Federica Pellati, Nicoletta Galeotti
Summary: The prevalence of obesity is increasing, and there is an urgent need for safe and efficient anti-obesity treatments. This study investigated the efficacy of a methanolic extract from Helichrysum stoechas, known for its anti-inflammatory properties, and its main constituent arzanol. The extract limited weight gain and comorbidities and showed potential as a therapeutic option for obesity and mood disorders.
PHYTOTHERAPY RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Maria Talmon, Erika Massara, Martina Quaregna, Marta De Battisti, Francesca Boccafoschi, Giulia Lecchi, Federico Puppo, Michele A. Bettega Cajandab, Stefano Salamone, Enrica Bovio, Renzo Boldorini, Beatrice Riva, Federica Pollastro, Luigia G. Fresu
Summary: This study demonstrates, for the first time, the presence and functionality of a type 2 bitter receptor hTAS2R46 in human skeletal muscle cells. This finding suggests that bitter receptors may play an important role in the development, maintenance, and protection of muscle tissue functions.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Agnese Gugliandolo, Santino Blando, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Simone D'Angiolini
Summary: In this study, the potential protective effects of cannabinol (CBN) pre-treatment against mitochondria impairment in a Parkinson's disease model were evaluated. The results showed that CBN was able to counteract the changes in gene expression related to mitochondria function caused by neurodegeneration, particularly in the process of mitophagy mediated by PINK1/Parkin signaling pathway.