Article
Biochemistry & Molecular Biology
Lixue Mao, Lijie Ge, Xinchun Ye, Li Xu, Weina Si, Ting Ding
Summary: A gene encoding a protein similar to germin-like proteins (GLPs) was found in maize. The gene, named ZmGLP1, is expressed in different tissues during different growth stages and is mainly found in stems and leaves. Overexpression of ZmGLP1 in Arabidopsis enhances resistance to different pathogens by inducing the expression of signaling-related genes and increasing oxidative burst activity and JA contents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Qi Chen, Yan Jin, Zhonghua Zhang, Meng Cao, Guanyun Wei, Xiaorui Guo, Jian Zhang, Xueyan Lu, Zhonghua Tang
Summary: Soil salinization causes severe stress to plants, inhibits plant growth, and limits agricultural productivity. This study compares the responses of Suaeda salsa and Puccinellia tenuiflora communities to saline-alkali stress, revealing different strategies in ion transport and phenolic compound accumulation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Xiangzhe Zhou, Feng Liu, Nuomin Li, Yongqian Zhang
Summary: Excessive hydrogen peroxide causes oxidative stress in cells. In this study, the largest dityrosine crosslinking dataset in E. coli to date was created, identifying 71 dityrosine crosslinks and 410 dityrosine loop links on 352 proteins. The dityrosine crosslinks are mainly involved in regulating metabolic pathways in response to oxidative stress.
Article
Chemistry, Analytical
Anqi Lin, Quanyi Liu, Yihong Zhang, Quan Wang, Sirong Li, Bijun Zhu, Leiying Miao, Yan Du, Sheng Zhao, Hui Wei
Summary: This study develops a universal strategy to measure CAT and CAT-like activity, overcoming the limitations of current assays and facilitating insightful studies on applications of CAT and CAT-like nanozymes. The oxygen-sensitive and H2O2-inhibitory self-polymerization of dopamine is demonstrated as an activity indicator for CAT or CAT-like nanozymes. This assay shows allround advantages in sensitivity, specificity, and versatility.
ANALYTICAL CHEMISTRY
(2022)
Review
Plant Sciences
Sheng Zheng, Min Su, Lu Wang, Tengguo Zhang, Juan Wang, Huichun Xie, Xuexia Wu, Syed Inzimam Ul Haq, Quan-Sheng Qiu
Summary: Cold stress adversely affects plant growth and crop yields in the Qinghai-Tibet Plateau, but plants have evolved mechanisms to overcome it by utilizing small signaling molecules for cellular signal transduction. These molecules play crucial roles in inducing gene expression, activating hormone signaling, and improving physiological functions to help plants tolerate cold stress.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Editorial Material
Multidisciplinary Sciences
Ferdi L. Hellweger, Charlotte Schampera, Robbie M. Martin, Falk Eigemann, Derek J. Smith, Gregory J. Dick, Steven W. Wilhelm
Summary: In response to the criticism by Huisman et al., this article demonstrates that their claims are based on incomplete evidence, misinterpretation of data, or do not actually refute our model. While our model, like any ecosystem models, has its simplifications and uncertainties, it outperforms existing approaches that ignore biology and cannot predict toxin concentration.
Review
Biochemistry & Molecular Biology
Ilaria Camponeschi, Arianna Montanari, Cristina Mazzoni, Michele Maria Bianchi
Summary: Living organisms on the surface biosphere are regularly exposed to light, which has led to the development of protective responses in yeasts against its deleterious effects. Light stress is propagated through the synthesis of hydrogen peroxide and regulated by factors also involved in response to other stressors. This suggests that light stress is a common factor in the yeast environmental response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Feijie Liu, Yueqiao Wang, Guhua Zhang, Longna Li, Wenbiao Shen
Summary: This study found that endogenous hydrogen gas (H-2) can promote lateral root (LR) formation, and this process is likely regulated through cell cycle regulation and auxin-dependent pathway.
Article
Cell Biology
Bjoern Klabunde, Andre Wesener, Wilhelm Bertrams, Stephan Ringshandl, Luke D. Halder, Evelyn Vollmeister, Bernd Schmeck, Birke J. Benedikter
Summary: Streptococcus pneumoniae infection disrupts the Golgi apparatus through H2O2 production, inhibiting anti-infective mechanisms and promoting bacterial attachment and replication.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biotechnology & Applied Microbiology
Feng Liu, Rui Min, Jie Hong, Guangqin Cheng, Yongqian Zhang, Yulin Deng
Summary: The study investigated the impact of endogenous oxidative stress caused by submicromolar hydrogen peroxide in a mutant strain of E. coli. Through label-free quantitative proteomic analysis, 265 differentially expressed proteins were identified, activating DNA repair and cellular antioxidant signaling pathways. These findings highlight the role of oxidative signaling and the potential of mutant E. coli as a cell model for studying oxidative stress effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
A. Ledo, E. Fernandes, A. Salvador, J. Laranjinha, R. M. Barbosa
Summary: Hydrogen peroxide is a significant redox signaling molecule that plays a crucial role in cell function and communication. In the brain, it acts as a signaling molecule and neuromodulator. Our study reveals that hydrogen peroxide can diffuse in the extracellular space and has a relatively short half-life. These quantitative details provide valuable insights into the physiology of redox signaling and lay the foundation for studying dysregulation in redox homeostasis associated with diseases.
Article
Engineering, Environmental
Yuan Cheng, Xin Li, Ming-Ya Fang, Qing-Jing Ye, Zhi-Miao Li, Golam Jalal Ahammed
Summary: The application of EECCG alleviated the adverse effects of cadmium stress in tomato plants by reducing oxidative stress and promoting the expression of RBOH1 gene. These findings contribute to our understanding of the stress resistance properties of EGCG in plants.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Physical
Do-Yeon Lee, Minju Park, Namhee Kim, Minsu Gu, Hyoung-il Kim, Byeong-Su Kim
Summary: This study presents a novel method for green and sustainable H2O2 production using dopamine-based materials, demonstrating the conversion of H2O2 generation mechanism from a chemical to a photocatalytic process along with the structural transformation of dopamine to polydopamine. The results showed that dopamine and polydopamine can both effectively generate H2O2, providing a new approach for the development of organic-based solar-to-H2O2 conversion and sustainable energy systems in the future.
JOURNAL OF CATALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Yubo Sun, Xiangrong Tian, Hao Wu, Xinchang Hao, Baowei Gao, Hongyan Zhang, Juntao Feng, Lirong Han
Summary: GP-1 is a glycoprotein elicitor found in Streptomyces kanasensis zx01 with antiviral activity, and it induces programmed cell death (PCD) in tobacco suspension cells, modulated by hydrogen peroxide (H2O2) and participating in biologically relevant signaling in plant cells.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Po-Kai Hsu, Yohei Takahashi, Ebe Merilo, Alex Costa, Li Zhang, Klara Kernig, Katie H. Lee, Julian Schroeder
Summary: The study investigated the kinetics of high VPD-induced stomatal responses, revealing that SLAC1 plays a more significant role in stomatal VPD signaling compared to ALMT12/QUAC1. The research also showed that VPD-induced stomatal closure is independent of the CO2 signal-transduction pathway. Calcium imaging demonstrated transient changes in cytoplasmic Ca2+ in guard cells under osmotic stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Yusen Zhou, Xiaomin Zhang, Jing Chen, Xiaopeng Guo, Hongyan Wang, Weibo Zhen, Junli Zhang, Zhubing Hu, Xuebing Zhang, Jose Ramon Botella, Toshiro Ito, Siyi Guo
Summary: In this study, we identified AHL9 as a positive regulator of leaf senescence in Arabidopsis thaliana. AHL9 accelerates leaf senescence through ethylene synthesis or signaling.
Article
Plant Sciences
Ryan Kilburn, Suzanne A. Gerdis, Yi-Min She, Wayne A. Snedden, William C. Plaxton
Summary: Phosphoenolpyruvate carboxylase (PEPC) plays a crucial role in plant metabolism. In castor oil seeds, a bacterial-type PEPC forms a complex with Ricinus communis Ca2+-dependent protein kinase-1 (RcCDPK1) which regulates its activity through phosphorylation. The autophosphorylation of RcCDPK1 facilitates its interaction with PEPC and regulates its transphosphorylation activity.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Junhua Li, Jinjin Guo, Chenchen Wei, Yao Meng, Xiaoduan Wang, Panpan Yu, Lin Yang, Yi Liang, Siyi Guo, Jinhong Yuan
Summary: The development of genetic and genomic resources for biological studies in cucumber has led to a boom in recent years. This study provides cytological techniques for studying meiosis in cucumber, including identification of meiotic stages using hierarchical criteria and the development of a simplified fluorescence in situ hybridization (FISH) protocol. Comparative analysis of cytoplasmic organelle distribution between cucumber and Arabidopsis is also presented.
Article
Biotechnology & Applied Microbiology
Yang Li, Heng Zhang, Yongxue Zhang, Yanshuang Liu, Yueyue Li, Haodong Tian, Siyi Guo, Meihong Sun, Zhi Qin, Shaojun Dai
Summary: In this study, the BSK gene family in spinach was analyzed, and the role of BSKs in metabolic processes was investigated. The results showed that SoBSKs in spinach had relatively conserved gene structure and protein function domains, and played important roles in signal transduction, redox regulation, and protein turnover. SoBSKs were found to respond to various abiotic stresses and phytohormones, and were highly expressed in leaves. These findings provide a foundation for further studying the regulatory mechanisms of BSK in spinach development and stress response.
Article
Biochemistry & Molecular Biology
Yi-Min She, Kody Klupt, Grayson Hatfield, Zongchao Jia, Roger Y. Tam
Summary: Mannosidases are crucial enzymes that play diverse roles in cellular processes. We discovered unexpected β1-4-galactosidase activity in the α1-6-mannosidase, providing insights into its dual glycosidase specificity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Jianjun Zhang, Yang Xia, Dinghe Wang, Yamin Du, Yongwu Chen, Congcong Zhang, Juan Mao, Muyang Wang, Yi-Min She, Xinxiang Peng, Li Liu, Josef Voglmeir, Zuhua He, Linchuan Liu, Jianming Li
Summary: This study demonstrates that in rescuing bri1-5 protein degradation, AtEDEM1 plays a predominant role over AtEDEM2, while a mutation in ALG3 eliminates the inhibitory effect of irb1-1 on bri1-5 ERAD.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Virology
Janet T. Lin, Sarah Kirst, Stevan Cucic, Alexandra Klem, Yi-Min She, Andrew M. Kropinski, Hany Anany
Summary: A novel bacteriophage with high specificity and a long latent period was isolated from EHEC-enriched sewage, expanding our understanding of bacteriophage diversity.
Article
Multidisciplinary Sciences
Yi-Min She, Shaojun Dai, Roger Y. Tam
Summary: Glycosylation is an important attribute for assessing the manufacturing quality of mAbs. We developed a highly sensitive LC MS/MS method for comprehensive characterization of low-abundance non-human glycans and their structural isomers. Our findings reveal the structural diversity of non-human glycans and the sulfation of alpha-galactosylated glycans, providing an analytical method and candidate structures for quality control of therapeutic mAb products.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Dexin Qiao, Junya Wang, Min-Hui Lu, Cuiping Xin, Yiping Chai, Yuanyuan Jiang, Wei Sun, Zhenghong Cao, Siyi Guo, Xue-Chen Wang, Qi-Jun Chen
Summary: This study tested PEs optimized through three strategies in maize and demonstrated that the ePE5max system efficiently generated heritable mutations that conferred resistance to herbicides in maize target genes.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Fuyou Xiang, Wen-Cheng Liu, Xin Liu, Yuwei Song, Yu Zhang, Xiaojing Zhu, Pengtao Wang, Siyi Guo, Chun-Peng Song
Summary: During seed germination, plants utilize the CYP77A4 gene to catalyze the epoxidation of unsaturated fatty acids, balancing energy supply and reactive oxygen species (ROS) production to prevent oxidative damage.
Article
Plant Sciences
Yongqi Cui, Meiqing He, Jie Liu, Shuang Wang, Junli Zhang, Shiyi Xie, Zhubing Hu, Siyi Guo, Dawei Yan
Summary: The maize LSC gene, encoding a large subunit of ribonucleotide reductase, plays a crucial role in dNTP synthesis, subsidiary cell development, and plant growth. The loss of subsidiary cells in the maize lsc mutant is attributed to impaired subsidiary mother cell polarization and division. In addition to the defect in subsidiary cells, the lsc mutant exhibits a dwarf morphology and pale and striped newly-grown leaves. Our findings indicate that LSC is essential for dNTP production, subsidiary mother cell polarization, subsidiary cell differentiation, and overall maize growth.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Correction
Plant Sciences
Yuebin Jia, Xiangpei Kong, Kongqin Hu, Mengqiang Cao, Jiajia Liu, Changle Ma, Siyi Guo, Xianzheng Yuan, Shan Zhao, Helene S. Robert, Cuiling Li, Huiyu Tian, Zhaojun Ding
Article
Plant Sciences
Chengyun Wu, Xiaoqing Wang, Weibo Zhen, Yaqing Nie, Yan Li, Penglai Yuan, Qiaoqiao Liu, Siyi Guo, Zhenguo Shen, Binglian Zheng, Zhubing Hu
Summary: The degradation of lariat intronic RNAs (lariRNAs) modulated by SICKLE (SIC) is essential for lateral root development in Arabidopsis. SIC interacts with DBR1 and facilitates its nuclear accumulation, leading to lariRNA decay and fine-tuning of miRNA biogenesis, thereby regulating lateral root development.
Article
Biochemistry & Molecular Biology
Shan Jiang, Jinwei Wei, Na Li, Zhibiao Wang, Yilan Zhang, Ran Xu, Lixun Zhou, Xiahe Huang, Li Wang, Siyi Guo, Yingchun Wang, Chun-Peng Song, Wei Qian, Yunhai Li
Summary: This study identifies a regulatory cascade involving UBP14/DA3-CDKB1;1-CDKG2/SUD6 that plays a crucial role in controlling endoreduplication and cell growth in Arabidopsis.
Article
Biochemistry & Molecular Biology
Guiling Sun, Mingzhang Xia, Jieping Li, Wen Ma, Qingzeng Li, Jinjin Xie, Shenglong Bai, Shanshan Fang, Ting Sun, Xinlei Feng, Guanghui Guo, Yanli Niu, Jingyi Hou, Wenling Ye, Jianchao Ma, Siyi Guo, Hongliang Wang, Yu Long, Xuebin Zhang, Junli Zhang, Hui Zhou, Baozhu Li, Jiong Liu, Changsong Zou, Hai Wang, Jinling Huang, David W. Galbraith, Chun-Peng Song
Summary: A cell atlas of maize peels and leaf bases created using single-nucleus RNA sequencing provides insights into the molecular mechanisms of stomatal movement and development in grasses. The study identified differentially expressed genes in guard cells and subsidiary cells, which are involved in various signaling pathways and affect cell wall plasticity. Overall, this research lays a comprehensive and fundamental foundation for understanding grass stomatal function.