Article
Biochemical Research Methods
Fei-Fan Tan, Rui Zhu, Bin Xiong, Gui-Min Zhang, Wei Zhao, Kai-Zhi Jia
Summary: The study engineered the entrance of an enzyme to improve substrate recognition and enzymatic activity. By manipulating the positions of key residues and the size of the entrance, as well as hydrophobic and electrostatic interactions, the study successfully increased the affinity and conversion rate between the enzyme and substrate.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mengyuan Wang, Changliang Yao, Jiayuan Li, Xuemei Wei, Meng Xu, Yong Huang, Quanxi Mei, De-an Guo
Summary: This study proposed an integrated approach combining column separation, mass spectrometry post-processing, and result verification for the identification of flavonoids in Dalbergia odorifera. A total of 197 flavonoids were characterized, with 7 compounds unambiguously identified in level 1.
Article
Multidisciplinary Sciences
David Elser, David Pflieger, Claire Villette, Baptiste Moegle, Laurence Miesch, Emmanuel Gaquerel
Summary: This study used a computational mass spectrometry-based metabolomics approach to explore the diversity of specialized metabolites (SM) in tissues of 20 species of the Nicotiana genus. By creating a large in silico fragmentation database and scripts for class prediction, the study provides an unprecedented mapping of SM diversity in this genus. The data integration approaches introduced here can serve as a valuable resource for future research on the evolution of plant SM.
Review
Food Science & Technology
Yuqing Tian, Wei Xu, Cuie Guang, Wenli Zhang, Wanmeng Mu
Summary: This review provides a comprehensive overview of the O-glycosylation of flavonoids catalyzed by glycoside hydrolases, including their catalytic mechanism, reaction conditions, and physicochemical properties of the product flavonoid glycosides. This biosynthesis approach offers a practical method to enhance glycodiversification due to its cheap glycosyl donor substrates and high yields.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Plant Sciences
Ximing Che, Bessie L. L. Splitt, Magnus T. T. Eckholm, Nathan D. D. Miller, Edgar P. P. Spalding
Summary: Gravitropism is crucial for plant growth above and below ground. Mutations in LAZY1 disrupt stem gravitropism and result in wider angles of inflorescence branches. The interaction between BRXL4 and LAZY1 at the plasma membrane negatively regulates LAZY1 function, affecting shoot system architecture.
Article
Biochemistry & Molecular Biology
Shiv Mani Dubey, Soeun Han, Nathan Stutzman, Michael J. Prigge, Eva Medvecka, Matthieu Pierre Platre, Wolfgang Busch, Matyas Fendrych, Mark Estelle
Summary: The phytohormone auxin triggers root growth inhibition within seconds via a non-transcriptional pathway. The AFB1 protein plays a crucial role in this rapid response, and its N-terminal region, including the F-box domain and residues contributing to auxin binding, is essential for its specific function. A substitution of the N-terminal region of AFB1 disrupts its cytoplasmic localization and activity in rapid root growth inhibition. Moreover, AFB1 is involved in calcium influx triggered by auxin and negatively regulates lateral root formation and transcription of auxin-induced genes.
Article
Biochemistry & Molecular Biology
Houjun Zhou, Haiman Ge, Jiahong Chen, Xueqin Li, Lei Yang, Hongxia Zhang, Yuan Wang
Summary: This study reveals the mechanism by which high concentration of SA affects gravitropic root growth and root hair development in plants. The regulation of PIN2 gene transcription and endocytosis of PIN2 protein are involved in this process. Exogenous SA application inhibits gravitropic root growth and root hair development by affecting auxin accumulation and PIN2 distribution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Liangqin Xie, Zeyuan Deng, Jie Zhang, Huanhuan Dong, Wei Wang, Banghuai Xing, Xiaoru Liu
Summary: This study compared the differences between flavonoid O-glycoside and C-glycoside in terms of their antioxidant activities and metabolism. The aglycones had higher activity but unstable structures, while the sugar substituents reduced the activity and improved stability. Moreover, C-glycoside was more stable than O-glycoside, which contributed to their differences in absorption and metabolism in vivo.
Article
Pharmacology & Pharmacy
Pengfei He, Sha Wang, Sen Li, Siqi Liu, Shuqi Zhou, Jing Wang, Jiayue Tao, Dongdong Wang, Rufeng Wang, Wenfu Ma
Summary: Recently, scientists have discovered an enzyme called DgpA/B/C from a human intestinal bacterium that can specifically cleave the C-glycosidic bond of puerarin and also has the activity as a structure isomerase. Through crystal structure and biochemical characterization, they have revealed how DgpA recognizes and oxidizes puerarin and proposed a possible mechanistic model. These findings not only contribute to the understanding of enzyme-mediated C-glycosidic bond breakage and formation, but also have the potential to facilitate stereospecific C-glycoside synthesis in the pharmaceutical industry.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Chemistry, Applied
Jinqian Yu, Wenxiu Chen, Daijie Wang, Lihua Xu, Xiao Wang
Summary: Four new compounds were isolated from Panax quinquefolius, and their structures were elucidated. These compounds exhibited cytotoxic activity against human cancer cell lines, with compound 1 showing the strongest targeted inhibitory effect on HCT116 cells.
NATURAL PRODUCT RESEARCH
(2023)
Article
Multidisciplinary Sciences
Zhenwei Zhang, Danie van Ophem, Raghunath Chelakkot, Naftali Lazarovitch, Ido Regev
Summary: This paper proposes a mechano-sensing model to explain the root-waving phenomenon in Arabidopsis, which combines the friction induced by gravitropism, the elasticity of the root, and the anchoring of the root to agar by thin hairs. By performing experiments and analyzing the results, the researchers demonstrate that the model can explain the effect of inclination angles on the wavelength and other properties of growing roots. They also show that the prevalence of waving in different plant species is related to root thickness, as predicted by the model.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Song An, Maho Yamashita, Sho Iguchi, Taketo Kihara, Eri Kamon, Kazuya Ishikawa, Masaru Kobayashi, Takeshi Ishimizu
Summary: This study identified two enzymes, PcGlcT and PcApiT, involved in the synthesis of flavonoid glycoside apiin in parsley. Their expression profiles were closely correlated with the accumulation of flavonoid 7-O-apiosylglucosides in parsley organs and developmental stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Kijong Song, Dae-Woo Lee, Jeongheon Kim, Jaewook Kim, Hwanuk Guim, Keunhwa Kim, Jong-Seong Jeon, Giltsu Choi
Summary: The research discovered that the ESV1 protein plays a role in promoting gravitropic responses in plants by regulating the formation of starch granules for hypocotyl negative gravitropism. In both Arabidopsis and rice, ESV1 mutants showed reduced levels of starch accumulation in the root columella and weakened root gravitropism.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Applied
Nguyen Thi Thuy Linh, Trinh Thi Thuy, Nguyen Thanh Tam, Ba Thi Cham, Khieu Thi Tam, Nguyen Hoang Sa, Do Thi Thao, Vu Tien Chinh, Nguyen Thi Hoang Anh
Summary: Sixteen compounds were isolated from Impatiens chapaensis, with seven showing alpha-glucosidase inhibition activity. Among them, flavonoid 5 exhibited the most significant inhibitory effect.
NATURAL PRODUCT RESEARCH
(2022)
Article
Agriculture, Multidisciplinary
Jiajing Chen, Gu Li, Haipeng Zhang, Ziyu Yuan, Wenyun Li, Zhaoxin Peng, Meiyan Shi, Wenyu Ding, Huixian Zhang, Yunjiang Cheng, Jia-Long Yao, Juan Xu
Summary: The study found a close correlation between the bitterness of citrus fruits and the presence of the 1,2RhaT gene, with pummelo identified as the genetic origin for FNH bitterness in modern citrus cultivars. Furthermore, certain transcription factor genes were shown to coexpress with genes related to FNH accumulation, potentially affecting the taste of citrus fruits.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
David. S. Letham, Xue-Dong Zhang, Charles H. Hocart
Article
Biochemistry & Molecular Biology
Karen J. Marsh, Isha Saraf, Charles H. Hocart, Kara Youngentob, Inder-Pal Singh, William J. Foley
Review
Physiology
Yasir Arfat, Andleeb Rani, Wang Jingping, Charles H. Hocart
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2020)
Article
Plant Sciences
Kelly Chapman, Ariel Ivanovici, Michael Taleski, Craig J. Sturrock, Jason L. P. Ng, Nadiatul A. Mohd-Radzman, Florian Frugier, Malcolm J. Bennett, Ulrike Mathesius, Michael A. Djordjevic
Article
Biochemical Research Methods
Zhenyu Zhu, Xijie Yin, Fengyan Lu, Bo Wang, Ran Ma, Yu Zhao, Ying Wang, Yi Ma, Jing Su, Qiulin Yan, Charles H. Hocart, Youping Zhou
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2020)
Article
Chemistry, Applied
Jinfeng Peng, Yang Yang, Youping Zhou, Charles H. Hocart, Hong Zhao, Yawei Hu, Feng Zhang
Article
Plant Sciences
Michael Taleski, Kelly Chapman, Nijat Imin, Michael A. Djordjevic, Michael Groszmann
Article
Multidisciplinary Sciences
Carole Laffont, Ariel Ivanovici, Pierre Gautrat, Mathias Brault, Michael Anthony Djordjevic, Florian Frugier
NATURE COMMUNICATIONS
(2020)
Article
Forestry
Lingling Zhu, Hongcheng Li, Michael R. Thorpe, Charles H. Hocart, Xin Song
Summary: Stomatal and mesophyll conductance were found to be the main limitations to photosynthesis in temperate and tropical tree species under heat stress during severe drought. The study highlights the importance of balancing water conservation and transpirational cooling in the diffusive pathway to respond to the combination of water and heat stress in forest trees.
TREES-STRUCTURE AND FUNCTION
(2021)
Article
Biotechnology & Applied Microbiology
Andleeb Rani, Yasir Arfat, Rao Salman Aziz, Liaqat Ali, Hammad Ahmed, Shazia Asim, Muhammad Rashid, Charles H. Hocart
Summary: Enhanced extraction of bioactive compounds from radish was achieved using enzymic pre-treatment, with optimal conditions producing high levels of antioxidants for potential commercial exploitation.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Plant Sciences
Han-Chung Lee, Steve Binos, Kelly Chapman, Sacha B. Pulsford, Ariel Ivanovici, John P. Rathjen, Michael A. Djordjevic
Summary: This study identified CRA2 as the Medicago CEP receptor using formaldehyde or photoactivation cross-linking methods, and found that sequence diversity within the CEP family influences receptor binding. Different CEP family peptides have distinct binding specificities.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Lingling Zhu, Wei Wen, Michael R. Thorpe, Charles H. Hocart, Xin Song
Summary: This study investigated the effects of combined heat stress and pre-existing drought on photosystem II in different plant species, showing that the combination exacerbated the effects on PSII photochemistry. The findings provide direction for future research and applications in agriculture and forestry under climate change.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Ariel Ivanovici, Carole Laffont, Estibaliz Larrainzar, Neha Patel, Courtney S. Winning, Han-Chung Lee, Nijat Imin, Florian Frugier, Michael A. Djordjevic
Summary: Legumes acquire soil nutrients through nitrogen-fixing root nodules and lateral roots. The hormone SymCEP7 promotes nodule formation without affecting lateral root number. This study reveals how plants coordinate root nodulation and lateral root development to procure nutrients.
Article
Plant Sciences
Kelly Chapman, Michael Taleski, Manuel Frank, Michael A. Djordjevic
Summary: Root system architecture plays a crucial role in acquiring soil nutrients. The hormones cytokinin and CEP influence root system architecture by controlling the angle of lateral root growth. This study reveals that cytokinin and CEP intersect at the downstream components CEPD1 and CEPD2 to promote shallower lateral root growth, which affects resource capture from the soil.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Michael Taleski, Marvin Jin, Kelly Chapman, Katia Taylor, Courtney Winning, Manuel Frank, Nijat Imin, Michael A. Djordjevic
Summary: Peptide hormones play crucial roles in plant growth, development, and environmental responses. Recent studies have provided insight into how CEP signaling operates, its interactions with diverse downstream targets, and its roles in nitrogen demand signaling, root system architecture, and nutrient uptake.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)