Article
Biochemistry & Molecular Biology
Maxim A. Khomutov, Arthur I. Salikhov, Vladimir A. Mitkevich, Vera L. Tunitskaya, Olga A. Smirnova, Sergey P. Korolev, Alexander O. Chizhov, Marina B. Gottikh, Sergey N. Kochetkov, Alex R. Khomutov
Summary: The biogenic polyamines, spermidine (Spd) and spermine (Spm), are essential for regulating vital cellular functions and are present in high concentrations in all eukaryotic cells. In this study, convenient gram-scale syntheses of C-methylated analogs of Spd were described, which could be used to investigate the cellular functions of polyamines and their metabolism. The biochemical properties of these compounds and the potential for regulating their activity by modifying their structure were discussed.
Article
Marine & Freshwater Biology
Xinxin Lu, Kai Wang, Xiaozhen Mou
Summary: Short-chained aliphatic polyamines are important carbon, nitrogen, and energy sources for marine bacterioplankton. Metagenomic responses of bacterioplankton in the Gulf of Mexico to different PA compounds show that the transformations are carried out by a broad range of taxa and have diverse pathways. PA-transforming genes and taxa vary among different sites, but remain similar among individual compound-amended metagenomes.
MARINE LIFE SCIENCE & TECHNOLOGY
(2022)
Article
Food Science & Technology
Caroline Liboreiro Paiva, Dea A. M. Netto, Valeria A. V. Queiroz, Maria Beatriz A. Gloria
Summary: This study quantified the levels of free bioactive amines during germination of tannin and tannin-free sorghum seeds and seedlings. It was found that tannin-free sorghum seeds had higher levels of spermidine, spermine, putrescine, and total amines compared to tannin sorghum. Throughout germination, putrescine was prevalent followed by spermidine. Spermine was only detected in the 7th germination day in tannin sorghum. The findings suggest that by selecting different genotypes, the polyamine contents in germinated sorghum can be modulated for specific food applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Silviya Ruseva, Inna Popova, Valentin Lozanov, Vanio Mitev
Summary: The study examined levels of Met, Hcy, and polyamines in psoriasis patients, finding correlations between them. Significant increases in Met and polyamine concentrations were observed in psoriatic plaques. The results highlight the importance of a balanced protein diet and intake of vitamins B-12 and B-9 in psoriasis treatment.
Article
Biochemistry & Molecular Biology
Longlong Wang, Jianjun Liang, Yu Zhou, Tao Tian, Baoli Zhang, Deqiang Duanmu
Summary: In this study, 13 carbonic anhydrase (CA) genes were identified in the model legume Lotus japonicus, with three of them further characterized for their expression patterns during nodule development. Knockout experiments using CRISPR/Cas9 technology showed that the knocked-out genes were not essential for nitrogen fixation under normal symbiotic conditions. The diverse expression patterns and distributions of these genes suggest their potential functions in symbiotic nitrogen fixation, awaiting further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Anastasia Mantziari, Enni Mannila, Maria Carmen Collado, Seppo Salminen, Carlos Gomez-Gallego
Summary: The study evaluated the effects of exogenous polyamines on the adhesive properties of various bacterial strains to human intestinal mucus, finding that polyamines may modulate bacterial adhesion to mucus depending on the bacterial strain and the age at which the mucus is generated.
Article
Biochemistry & Molecular Biology
Orsolya Kinga Gondor, Judit Tajti, Kamiran Aron Hamow, Imre Majlath, Gabriella Szalai, Tibor Janda, Magda Pal
Summary: This study aimed to investigate the influence of polyamine metabolism under different light conditions. It was found that polyamines had varying effects on photosynthesis under different light regimes, suggesting a complex relationship between polyamines and plant adaptation to light conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Nelly C. Munoz-Esparza, Oriol Comas-Baste, M. Luz Latorre-Moratalla, M. Teresa Veciana-Nogues, M. Carmen Vidal-Carou
Summary: Human milk is rich in polyamines, which are essential for intestinal maturation and immune system development, while infant formulas have significantly lower levels of polyamines compared to human milk. Spermidine and spermine levels in human milk decrease over lactation, while putrescine remains relatively constant.
Article
Nutrition & Dietetics
Nelly C. Munoz-Esparza, Edgar M. Vasquez-Garibay, Elizabeth Guzman-Mercado, Alfredo Larrosa-Haro, Oriol Comas-Baste, M. Luz Latorre-Moratalla, M. Teresa Veciana-Nogues, M. Carmen Vidal-Carou
Summary: The study evaluated the influence of different breastfeeding factors on the polyamine levels in human milk, finding that polyamine levels varied significantly between mothers and tended to decrease over time. Spermidine and spermine concentrations were higher in hindmilk compared to foremilk, and decreased significantly as lactation progressed.
Review
Biochemistry & Molecular Biology
Guadalupe Elizabeth Jimenez Gutierrez, Fabiola V. Borbolla Jimenez, Luis G. Munoz, Yessica Sarai Tapia Guerrero, Nadia Mireya Murillo Melo, Jose Melesio Cristobal-Luna, Norberto Leyva Garcia, Joaquin Cordero-Martinez, Jonathan J. Magana
Summary: Polyamines are short molecules with two or three amine groups that play an important role in age-related diseases. This review provides a new perspective on the involvement of polyamines in cellular and molecular processes related to these diseases, suggesting their potential as novel biomarkers for healthier aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Letizia Galasso, Annalisa Cappella, Antonino Mule, Lucia Castelli, Andrea Ciorciari, Alessandra Stacchiotti, Angela Montaruli
Summary: Autophagy dysregulation is common in musculoskeletal diseases. Polyamines, such as spermidine and spermine, have anti-aging effects and can reverse dysfunctional autophagy. Supplementation of polyamines may be important in preventing or treating muscle atrophy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Rakesh K. Upadhyay, Jonathan Shao, Autar K. Mattoo
Summary: Identification of the polyamine biosynthetic pathway genes in duckweed S. polyrhiza shows the presence of prokaryotic as well as land plant-type ADC pathway but absence of ODC encoding genes. Differential gene expression and transcript abundance of these genes are modulated by exogenous methyl jasmonate, salinity, and acidic pH. The S. polyrhiza genome encodes for complete polyamine biosynthesis pathway and the genes are transcriptionally active in response to changing environmental conditions, suggesting an important role of polyamines in this aquatic plant.
Review
Biochemistry & Molecular Biology
Abhishek Kulkarni, Cara M. Anderson, Raghavendra G. Mirmira, Sarah A. Tersey
Summary: This review summarizes the existing research on polyamine-hypusine metabolism in the context of beta-cell function and diabetes pathogenesis.
Review
Biochemistry & Molecular Biology
Julian Rieck, Serguei N. Skatchkov, Christian Derst, Misty J. Eaton, Rudiger W. Veh
Summary: This review provides a comprehensive summary of the characteristics and biological functions of polyamines, including some new aspects and questions, covering their roles, uptake mechanisms, concentration differences, biosynthesis, and transport.
Article
Food Science & Technology
Nelly C. Munoz-Esparza, Judit Costa-Catala, Oriol Comas-Baste, Natalia Toro-Funes, M. Luz Latorre-Moratalla, M. Teresa Veciana-Nogues, M. Carmen Vidal-Carou
Summary: Research has shown that different foods contain varying levels of polyamines, with plant-based foods such as mushrooms and citrus fruits, and animal-based foods such as cheese and sausages, containing higher levels. The study also found that different cooking processes can impact polyamine levels, with boiling and grilling reducing polyamine levels, while microwave and sous-vide cooking methods have minimal effect on polyamine content.
Article
Biotechnology & Applied Microbiology
Chrysanthi Pateraki, Dimitrios Skliros, Emmanouil Flemetakis, Apostolis Koutinas
Summary: This study evaluated the metabolic response of Actinobacillus succinogenes for succinic acid production using spent sulphite liquor (SSL) as feedstock, derived from Eucalyptus globulus hardwood. Transcriptomic analysis revealed significant upregulation of genes related to major metabolic pathways in batch cultures with SSL compared to glucose and xylose.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Na Ding, Raul Huertas, Ivone Torres-Jerez, Wei Liu, Bonnie Watson, Wolf-Rudiger Scheible, Michael Udvardi
Summary: This study found that switchgrass responds to phosphorus limitation by affecting overall plant growth, root/shoot ratio, root branching and gene transcription, with stress-induced genes involved in transcriptional regulation, metabolism, and transport processes associated with phosphorus acquisition and homeostasis. Metabolite profiling also revealed changes in levels of sugars, organic acids and amino acids under different levels of phosphorus stress.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Sonia Torres, Carmen Lama, Lalia Mantecon, Emmanouil Flemetakis, Carlos Infante
Summary: Quantitative real-time reverse transcription PCR (RT-qPCR) was used to analyze gene modulation in green microalgae Tetraselmis chui, with evaluation of 18 candidate reference genes using different algorithms under various culture conditions. The study identified specific reference genes for different culture conditions and highlighted the reliability of these genes for gene expression studies in T. chui. This research contributes to establishing an RT-qPCR platform for quality control of biomass production in industrial facilities.
Article
Plant Sciences
Sotirios Zerveas, Evangelos Kydonakis, Polychronis Moutidis, Aristeidis Maragkoudakis, Kiriakos Kotzabasis
Summary: The study demonstrates that microalgae can effectively increase oxygen levels in anoxic atmospheres by utilizing high concentrations of CO2 for photosynthesis, promoting growth without stress responses. This survival strategy of microalgae could potentially be used for combating the greenhouse effect and recycling atmospheres on other planets, providing a useful module for (astro)biotechnological applications.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Virology
Dimitrios Skliros, Efthymios Karpouzis, Chrysanthi Kalloniati, Pantelis Katharios, Emmanouil Flemetakis
Summary: Tailed bacteriophages with their peculiar contractile tail structure have drawn attention. Through comparative genomics analysis, a pattern of genomic organization and the importance of the baseplate protein encoding genomic region have been revealed. Additionally, the proposal of new genera for dwarf Vibrio myoviruses was made. Comparative genomics of phages infecting aquatic bacteria could provide valuable information for combating fish pathogens in aquaculture.
ARCHIVES OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Bikash Raul, Oindrila Bhattacharjee, Amit Ghosh, Priya Upadhyay, Kunal Tembhare, Ajeet Singh, Tarannum Shaheen, Asim Kumar Ghosh, Ivone Torres-Jerez, Nick Krom, Josh Clevenger, Michael Udvardi, Brian E. Scheffler, Peggy Ozias-Akins, Ravi Datta Sharma, Kaustav Bandyopadhyay, Vineet Gaur, Shailesh Kumar, Senjuti Sinharoy
Summary: The study reveals dynamic changes in plant gene expression during peanut nodule development using comprehensive analysis and transcriptomics. Compared to other model legumes, peanut demonstrates diversification in oxygen-scavenging mechanisms and molecular mechanisms of terminal bacteroid differentiation.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Plant Sciences
Sonali Roy, Marcus Griffiths, Ivone Torres-Jerez, Bailey Sanchez, Elizabeth Antonelli, Divya Jain, Nicholas Krom, Shulan Zhang, Larry M. York, Wolf-Ruediger Scheible, Michael Udvardi
Summary: This study describes a high throughput phenotyping platform for testing the effects of small signaling peptides (SSP) on root uptake of multiple nutrients in plants. The results show that SSP CEP1 can enhance nitrate uptake rate in Medicago truncatula plants deprived of nitrogen. Furthermore, specific structural variants of CEP1 peptides not only enhance nitrate uptake, but also phosphate and sulfate uptake in both Medicago truncatula and Arabidopsis thaliana. Transcriptome analysis reveals that CEP1 peptides can induce the expression of several genes, including nutrient transporters and signaling pathway genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Yun Kang, Amaia Seminario, Michael Udvardi, Paolo Annicchiarico
Summary: This study aimed to explore the relationship between leaf morphophysiological traits and adaptive response in alfalfa, and it provided support for breeding strategies. The results showed that cultivars specifically adapted to drought-prone environments exhibited better drought tolerance and smaller leaves compared to those specifically adapted to moisture-favourable environments. In addition, the study identified some inexpensive morphophysiological traits that could be used for early selection targeting drought-prone environments.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2023)
Review
Plant Sciences
Eleni Navakoudis, Kiriakos Kotzabasis
Summary: This review highlights the bioenergetic role of polyamines in plant protection and development and proposes a universal model for describing polyamine-mediated stress responses. Polyamines can regulate the energy and structure of the photosynthetic apparatus and control the photochemical and non-photochemical quenching of energy. By altering the composition of polyamines, stress-sensitive plants can be made tolerant.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Cell Biology
Kashif Mahmood, Ivone Torres-Jerez, Nick Krom, Wei Liu, Michael K. Udvardi
Summary: This study used RNA-sequencing to investigate the molecular basis of age-dependent and dark-induced leaf senescence in Medicago truncatula, a forage crop. The results revealed the differentially expressed genes and identified the key biological processes associated with leaf senescence. The study also identified a set of transcription factors that promoted senescence in tobacco leaves when expressed. These findings provide valuable insights into the mechanisms of leaf senescence in forage crops and suggest potential targets for controlling senescence in these crops.
Article
Biology
Zhao Hao, Yuan Wang, Na Ding, Malay C. Saha, Wolf-Rudiger Scheible, Kelly Craven, Michael Udvardi, Peter S. Nico, Mary K. Firestone, Eoin L. Brodie
Summary: In this study, it was found that switchgrass adapts well to marginal soils with low phosphorus content. The research also discovered that phosphorus limitation affects the chemical composition and quality of switchgrass, and different soil conditions impact nutrient allocation strategies, which in turn affects biomass yields in the subsequent year. The use of a machine learning model effectively addresses these challenges and provides important information about switchgrass growth conditions and feedstock quality.
COMMUNICATIONS BIOLOGY
(2022)
Article
Plant Sciences
Aikaterini Koletti, Irene Dervisi, Chrysanthi Kalloniati, Maria-Eleftheria Zografaki, Heinz Rennenberg, Andreas Roussis, Emmanouil Flemetakis
Summary: This study identifies a selenium-binding protein, CrSBD1, as a cell regulator involved in modulating early responses to oxidative stress in Chlamydomonas reinhardtii. The absence of functional CrSBD1 leads to increased growth under mild oxidative stress conditions, but decreased cell viability at higher hydrogen peroxide concentrations. Transcriptomic and metabolomic analysis reveals a significant reduction in molecular and biochemical responses to H2O2-induced oxidative stress in the sbd1 mutant compared to the wild-type.
Editorial Material
Biochemistry & Molecular Biology
Nancy A. Eckardt, Sean Cutler, Thomas E. Juenger, Amy Marshall-Colon, Michael Udvardi, Paul E. Verslues
Article
Biotechnology & Applied Microbiology
Dimitrios Skliros, Polyxeni Papazoglou, Danai Gkizi, Eleni Paraskevopoulou, Pantelis Katharios, Dimitrios E. Goumas, Sotirios Tjamos, Emmanouil Flemetakis
Summary: In recent years, the biology and biotechnology of bacteriophages have been extensively studied as a means to find environmentally friendly methods to control phytopathogenic bacteria. The use of bacteriophages in biological control of Pseudomonas syringae pv. tomato (Pst) could be an alternative approach to combat bacterial speck disease in tomato plants. The isolated bacteriophage, Medea1, showed promising results in reducing Pst symptoms in tomato plants and upregulating defense-related genes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Irene Dervisi, Chrysanthi Valassakis, Aikaterini Koletti, Vassilis N. Kouvelis, Emmanouil Flemetakis, Christos A. Ouzounis, Andreas Roussis
Summary: Selenium-binding proteins (SBPs) are a widely distributed protein family, with SBP1 recently identified as a new stress response regulator in plants. Although SBP1 has been characterized as a methanethiol oxidase, its exact role is still unclear. In mammals, SBP1 is involved in anti-carcinogenic growth regulation, redox modulation, and detoxification. This study investigates the functional potential of specific motifs of SBPs in an evolutionary context. Phylogenetic analysis reveals the absence of SBP in fungi and most non-eukaryotic organisms. The phylogenetic tree also highlights the differentiation and evolution of characteristic SBP motifs, with modifications found in Acidobacteria, Fungi, and Archaea for the CSSC motif and a conserved CC motif in Plants modified to CxxC in Animals. These results suggest that SBPs likely emerged from bacteria as methanethiol oxidases.
JOURNAL OF MOLECULAR EVOLUTION
(2023)
