Article
Biochemistry & Molecular Biology
Mindaugas Lesanavicius, Daisuke Seo, Narimantas Cenas
Summary: RpFNR is a novel thioredoxin reductase-type FNR with partly characterized redox properties. It can be oxidized by various nonphysiological electron acceptors, and the reactivity is influenced by the single-electron reduction midpoint potential of the compounds.
Article
Biology
Manuela Kramer, Melvin Rodriguez-Heredia, Francesco Saccon, Laura Mosebach, Manuel Twachtmann, Anja Krieger-Liszkay, Chris Duffy, Robert J. Knell, Giovanni Finazzi, Guy Thomas Hanke
Summary: This study demonstrates the membrane association of FNR and its distribution depending on interaction with specific tether proteins. There is a correlation between FNR's interaction with different proteins and the activity of alternative photosynthetic electron transport pathways, supporting a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.
Article
Biochemistry & Molecular Biology
Yutaro Chikuma, Masayuki Miyata, Young-Ho Lee, Toshiharu Hase, Yoko Kimata-Ariga
Summary: This study investigated the molecular mechanism of negative cooperativity between FNR and Fd in plants, caused by the allosteric binding of NADP(H) on FNR. It identified a salt bridge between Asp60 of Fd and Lys33 of FNR as crucial for this negative cooperativity. Site-specific mutants of FdD60 and FNRK33 confirmed the involvement of this salt bridge in downregulating the interaction between FNR and Fd by NADPH.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Paula Monchietti, Arleth S. Lopez Rivero, Eduardo A. Ceccarelli, Daniela L. Catalano-Dupuy
Summary: FNRs are ubiquitous flavoenzymes involved in redox metabolisms, catalyzing electron transfer between NADP(H) and ferredoxin or flavodoxin. Plant-type FNRs are divided into plastidic and bacterial classes, with plastidic FNRs showing higher turnover numbers. Research on bacterial FNRs revealed a new model of NADP(+) binding and catalysis, highlighting its essential role in redox homeostasis.
Article
Multidisciplinary Sciences
Yuichi Yokochi, Keisuke Yoshida, Florian Hahn, Atsuko Miyagi, Ken-ichi Wakabayashi, Maki Kawai-Yamada, Andreas P. M. Weber, Toru Hisabori
Summary: The redox regulation of enzyme activity through cysteine residues in chloroplasts is crucial for maintaining NADPH homeostasis under different light conditions. A mutation that eliminates a redox switch in chloroplast NADP-malate dehydrogenase results in severe growth retardation in plants under short-day or fluctuating light conditions, highlighting the importance of fine-tuning the activity of key enzymes for efficient photosynthesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Yoko Kimata-Ariga, Karen Fukuta, Masayuki Miyata
Summary: In the chloroplast stroma, changes in pH affect the electron transfer activity between ferredoxin-NADP(+) reductase (FNR) and ferredoxin (Fd) isoproteins in maize leaves. Increasing pH leads to increased activity between FNR and photosynthetic-type Fd (Fd1), while activity with nonphotosynthetic type Fd (Fd3) decreases. The His78 amino acid residue in Fd1 plays a regulatory role in its interaction with FNR, as demonstrated by mutation studies.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Microbiology
Wanwipa Ittarat, Takeshi Sato, Masaharu Kitashima, Hidehiro Sakurai, Kazuhito Inoue, Daisuke Seo
Summary: Through studying the oxidoreductases in the genome of the green sulfur bacterium, it was found that rubredoxin (Rd) and a putative flavodiiron protein (FDP) may participate in the reduction of dioxygen, and the ferredoxin-NAD(P)(+) oxidoreductase (FNR) can promote the reduction of Rd.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Environmental Sciences
Mahire Bayramoglu Akkoyun, Yusuf Temel, Aydin Sukru Bengu, Hurrem Turan Akkoyun
Summary: Copper ions have inhibitory effects on rat erythrocyte pentose phosphate pathway and antioxidant system enzymes, which can be reduced by astaxanthin.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biophysics
Stanislav D. Zakharov, Sergei Savikhin, Yuko Misumi, Genji Kurisu, William A. Cramer
Summary: This study confirms the absence of a significant interaction between FNR and the b(6)f complex through thermodynamic analysis. It also provides a precedent for considering protein-detergent interactions in ITC analyses.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Hitesh Medipally, Marvin Mann, Carsten Koetting, Willem J. H. van Berkel, Marc M. Nowaczyk
Summary: Photosynthetic organisms use light for the regeneration of NADPH. In this study, a clickable fusion system was established to regenerate NADPH with PSI-Fd and PSI-Fd-FNR. Immunity protein 7 was fused to the PSI-PsaE subunit, and colicin DNase E7 fusion chimeras of Fd and FNR were expressed. The complexes showed the ability to regenerate NADPH, demonstrating the applicability of this strategy.
Article
Biochemistry & Molecular Biology
H. Doerpholz, S. Subramanian, A. Zouni, F. Lisdat
Summary: In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus were prepared and characterized. The direct electron transfer between PSI-Fd and the electrode was investigated. The results showed that the modified PSI-Fd photobioelectrode had a lower photocurrent and a lower onset potential of the cathodic photocurrent compared to native PSI, mainly due to the different adsorption behavior of the PSI-Fd construct onto the 3D ITO electrode. However, the overall behavior was similar to PSI. First experiments demonstrated the usage of the PSI-Fd photobioelectrode for enzyme-driven NADPH generation.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuxi Lin, So-Hyeon Park, Eugene Bok, Yunseok Heo, Seong-Bin Yang, Yoon-Sun Yi, Jun-Hyuck Lee, Donghyun Seo, Eunae Jo, Sungsu Lim, Yun Kyung Kim, Jozsef Kardos, Kyoung-Seok Ryu, Jaekwang Kim, Jooho Park, Young-Ho Lee
Summary: This study synthesized a novel amphiphilic sub-nanosized material, RR-BA, which showed specific binding to α SN through electrostatic interactions and induced amyloid fibrillation. RR-BA had no noticeable effect on the amyloid formation of K18 and Aβ42 due to weak and non-specific interactions.
Article
Chemistry, Medicinal
Bogdan Adam Cichocki, Maxime Donzel, Kim C. Heimsch, Mindaugas Lesanavicius, Liwen Feng, Enrique Jose Montagut, Katja Becker, Alessandro Aliverti, Mourad Elhabiri, Narimantas Cenas, Elisabeth Davioud-Charvet
Summary: Plasmodione (PD) is a potent antimalarial compound that acts on different stages of the malaria parasite at low nanomolar concentrations. The bioactivation of PD involves a cascade of redox reactions resulting in the generation of key metabolites. The oxidation of PD is closely related to the formation of a benzylic semiquinone radical, which can be produced under UV irradiation or catalysis by Plasmodium falciparum apicoplast ferredoxin-NADP(+) reductase.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Christine M. Lewis, Justin D. Flory, Thomas A. Moore, Ana L. Moore, Bruce E. Rittmann, Wim F. J. Vermaas, Cesar Torres, Petra Fromme
Summary: A novel microbial electro-photosynthetic system (MEPS) is presented, which uses redox mediators and electrodes to drive electron transport in live cells. The results show that light-dependent current can be generated at high light intensities, and the electron delivery rate exceeds that of wildtype photosystem II (PSII). Furthermore, electron delivery occurs before the cytochrome b(6)f complex, enabling the production of both NADPH and ATP.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Takashi Iyanagi
Summary: This article compares the roles of FNR-Fd and FNR-Fld complex pairs in plants with the diflavin reductase (FAD-FMN) family and discusses the evolutionary aspects of NAD(P)H-dependent multi-domain electron transfer systems.
Article
Plant Sciences
Jose A. Rojas-Gonzalez, Mauricio Soto-Suarez, Angel Garcia-Diaz, Maria C. Romero-Puertas, Luisa M. Sandalio, Angel Merida, Ina Thormaehlen, Peter Geigenberger, Antonio J. Serrato, Mariam Sahrawy
JOURNAL OF EXPERIMENTAL BOTANY
(2015)
Article
Plant Sciences
Mauricio Soto-Suarez, Antonio J. Serrato, Jose A. Rojas-Gonzalez, Rocio Bautista, Mariam Sahrawy
Article
Genetics & Heredity
Jarkko Salojarvi, Olli-Pekka Smolander, Kaisa Nieminen, Sitaram Rajaraman, Omid Safronov, Pezhman Safdari, Airi Lamminmaki, Juha Immanen, Tianying Lan, Jaakko Tanskanen, Pasi Rastas, Ali Amiryousefi, Balamuralikrishna Jayaprakash, Juhana I. Kammonen, Risto Hagqvist, Gugan Eswaran, Viivi Helena Ahonen, Juan Alonso Serra, Fred O. Asiegbu, Juan de Dios Barajas-Lopez, Daniel Blande, Olga Blokhina, Tiina Blomster, Suvi Broholm, Mikael Brosche, Fuqiang Cui, Chris Dardick, Sanna E. Ehonen, Paula Elomaa, Sacha Escamez, Kurt V. Fagerstedt, Hiroaki Fujii, Adrien Gauthier, Peter J. Gollan, Pauliina Halimaa, Pekka I. Heino, Kristiina Himanen, Courtney Hollender, Saijaliisa Kangasjarvi, Leila Kauppinen, Colin T. Kelleher, Sari Kontunen-Soppela, J. Patrik Koskinen, Andriy Kovalchuk, Sirpa O. Karenlampi, Anna K. Karkonen, Kean-Jin Lim, Johanna Leppala, Lee Macpherson, Juha Mikola, Katriina Mouhu, Ari Pekka Mahonen, Ulo Niinemets, Elina Oksanen, Kirk Overmyer, E. Tapio Palva, Leila Pazouki, Ville Pennanen, Tuula Puhakainen, Peter Poczai, Boy J. H. M. Possen, Matleena Punkkinen, Moona M. Rahikainen, Matti Rousi, Raili Ruonala, Christiaan van der Schoot, Alexey Shapiguzov, Maija Sierla, Timo P. Sipila, Suvi Sutela, Teemu H. Teeri, Arja I. Tervahauta, Aleksia Vaattovaara, Jorma Vahala, Lidia Vetchinnikova, Annikki Welling, Michael Wrzaczek, Enjun Xu, Lars G. Paulin, Alan H. Schulman, Martin Lascoux, Victor A. Albert, Petri Auvinen, Yka Helariutta, Jaakko Kangasjarvi
Article
Plant Sciences
Juan de Dios Barajas-Lopez, Jose Ramon Moreno, Francisco M. Gamez-Arjona, Jose M. Pardo, Matleena Punkkinen, Jian-Kang Zhu, Francisco J. Quintero, Hiroaki Fujii
Article
Plant Sciences
Valle Ojeda, Juan Manuel Perez-Ruiz, Maricruz Gonzalez, Victoria A. Najera, Mariam Sahrawy, Antonio J. Serrato, Peter Geigenberger, Francisco Javier Cejudo
Article
Biochemistry & Molecular Biology
Antonio Jesus Serrato, Maria C. Romero-Puertas, Alfonso Lazaro-Payo, Mariam Sahrawy
Article
Multidisciplinary Sciences
Zhenying Cai, Jingjing Liu, Haijiao Wang, Cangjing Yang, Yuxiao Chen, Yongchi Li, Shanjin Pan, Rui Dong, Guiliang Tang, Juan de Dios Barajas-Lopez, Hiroaki Fujii, Xuelu Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Plant Sciences
Lucia Perez, Erika Soto, Gemma Farre, Julia Juanos, Gemma Villorbina, Ludovic Bassie, Vicente Medina, Antonio Jesus Serrato, Mariam Sahrawy, Jose Antonio Rojas, Ignacio Romagosa, Pilar Munoz, Changfu Zhu, Paul Christou
PLANT CELL REPORTS
(2019)
Article
Plant Sciences
Antonio J. Serrato, Jose A. Rojas-Gonzalez, Diego Torres-Romero, Paola Vargas, Angel Merida, Mariam Sahrawy
Summary: The different isoforms of Thioredoxins (TRXs) play essential roles in light energy uptake and primary metabolism regulation in plants. Disruption of TRX m signalling can have harmful consequences on chloroplast metabolism and plant development levels. Furthermore, TRX m isoforms are involved in fine-tuning the response to high light conditions, such as regulating the accumulation of protective pigments like anthocyanin.
Article
Plant Sciences
Diego Torres-Romero, Angeles Gomez-Zambrano, Antonio Jesus Serrato, Mariam Sahrawy, Angel Merida
Summary: The study found that FBN proteins interact with each other in plant chloroplasts, interacting with allene oxide synthase, affecting photosynthesis and oxidative stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Maria-Cruz Gonzalez, Francisco Javier Cejudo, Mariam Sahrawy, Antonio Jesus Serrato
Summary: The article discusses mechanisms that allow an appropriate electron flux to avoid the detrimental consequences of photosynthesis redox imbalances.
Article
Biochemistry & Molecular Biology
Mariam Sahrawy, Juan Fernandez-Trijueque, Paola Vargas, Antonio J. Serrato
Summary: This study investigates the localization and function of Thioredoxins f and m in Arabidopsis. The results reveal different localization patterns of these proteins within chloroplasts and their presence in non-photosynthetic plastids and roots. Furthermore, the study suggests that TRXm2 might activate cFBP1 in root plastids.
Article
Biochemistry & Molecular Biology
Juan Fernandez-Trijueque, Antonio-Jesus Serrato, Mariam Sahrawy
Article
Biochemistry & Molecular Biology
Yanru Feng, Muhammad Shahedul Alam, Feng Yan, Michael Frei
Summary: Tropospheric ozone has significant effects on the remobilization and allocation efficiency of aboveground biomass and nutrients in cereal crops. Long-term ozone exposure increases straw C:N ratio and affects grain C:N ratio. Grain N concentrations increase significantly under ozone stress, but N yield declines due to grain yield losses. Various indicators of N use efficiency are reduced, indicating reduced N absorption from soil and allocation from vegetative to reproductive organs. Straw C:N ratio is not suitable for predicting wheat productivity. Nitrogen harvest index (NHI) is not affected by ozone stress, but the relationship between harvest index (HI) and NHI is changed by elevated ozone concentration.
Article
Biochemistry & Molecular Biology
Cong Guan, Wei Li, Guoliang Wang, Ruimei Yang, Jinglei Zhang, Jinhong Zhang, Bo Wu, Run Gao, Chunlin Jia
Summary: This study characterized the expression profiles of mRNAs and ncRNAs in switchgrass under drought stress. The up-regulated mRNAs were enriched in starch and sucrose metabolism pathway, while the differentially expressed lncRNAs potentially regulated protein-coding genes. The study also constructed regulatory networks and validated the functionality of the target gene PvSS4 in enhancing drought tolerance.
Article
Biochemistry & Molecular Biology
Juan Nicolas-Espinosa, Lucia Yepes-Molina, Fuensanta Martinez-Bernal, Miriam Fernandez-Pozurama, Micaela Carvajal
Summary: This study aimed to investigate the physiological response of broccoli leaves to abiotic stresses such as salinity and boron toxicity/deficiency. The results showed that the combined stress of salinity and boron deficiency resulted in a significant reduction in plant biomass, and the adaptation mechanisms were associated with water and boron concentration in the leaves. The expression patterns of PIP aquaporins varied among the different stress treatments, and their presence in the plasma membrane and interaction with the lipid environment played potential regulatory roles in facilitating salinity-boron stress adaptation mechanisms.
Review
Biochemistry & Molecular Biology
Wen-Feng Huang, Juan Li, Jian-An Huang, Zhong-Hua Liu, Li-Gui Xiong
Summary: This review examines the seasonal trends of phyllosphere microorganisms in woody and herbaceous plants and explores the factors influencing these trends. While herbaceous and woody plants share some similarities and differences in their phyllosphere microbiomes, further experimental validation is needed.
Article
Biochemistry & Molecular Biology
Changguang Liao, Hui Shen, Zihan Gao, Yunshu Wang, Zhiguo Zhu, Qiaoli Xie, Ting Wu, Guoping Chen, Zongli Hu
Summary: The novel CRF, SlCRF6, plays a crucial role in regulating tomato plant morphology, leaf development, and the accumulation of photosynthetic products.
Article
Biochemistry & Molecular Biology
Alok Madhu, Alok Sharma, Amandeep Kaur, Kashmir Singh, Santosh Kumar Upadhyay
Summary: In this study, 15 TaMDHAR genes were identified in bread wheat and their crucial roles in antioxidants, growth and development, and stress responses were revealed.
Article
Biochemistry & Molecular Biology
Kai Zheng, Yongsheng Cai, Yanying Qu, Lu Teng, Chaoyue Wang, Jie Gao, Quanjia Chen
Summary: In this study, the whole genome identification and bioinformatics analysis of the HCT gene family were performed in G. barbadense. The results showed that the GbHCT114 gene regulates plant trichome development, which is closely related to cotton fiber quality. Gene silencing and overexpression experiments confirmed the important role of GbHCT114 gene in cotton fiber morphology, lignin content, and secondary xylem duct cell wall development. Transcriptomic analysis identified differentially expressed genes associated with lignin synthesis and fiber development.
Review
Biochemistry & Molecular Biology
Tanashvi Seth, Sejal Asija, Shahid Umar, Ravi Gupta
Summary: Plants activate a sophisticated signaling cascade in response to pests and pathogens, with lipids playing a crucial role in mediating these defense responses. Different types of lipids are involved in cell signaling during plant-pathogen interaction and each lipid has specific relevance and contributes to specific signaling cascades. Lipid biosynthetic enzymes, including phospholipases, are involved in the production of defense signaling molecules. Lipids participate in stress signaling by mediating signal transduction, acting as precursors for bioactive molecules, regulating ROS formation, and interacting with phytohormones.
Article
Biochemistry & Molecular Biology
Yangyang Chen, Xiao Wu, Xiaohua Wang, Qionghou Li, Hao Yin, Shaoling Zhang
Summary: 'Nanguo' pears emit a rich aroma when fully ripe, and the important volatile components are the six-carbon compounds derived from the lipoxygenase pathway. This study identified a highly expressed bZIP transcription factor that is induced during the mature stage of 'Nanguo' pears, and demonstrated its regulatory role in fatty acid-derived volatile biosynthesis.
Article
Biochemistry & Molecular Biology
Zhao Geng, Haikuan Dou, Jianguang Liu, Guiyuan Zhao, Linlin Liu, Ning Zhao, Hanshuang Zhang, Yongqiang Wang, Zetong An
Summary: The overexpression of GhFB15 gene decreases the salt tolerance of Arabidopsis plants, while silencing the gene improves the salt tolerance of cotton plants. Furthermore, GhFB15 regulates the accumulation of flavonoids and the levels of ROS.
Article
Biochemistry & Molecular Biology
Linjun Cai, Ancheng Ma, Jiao Lei, Chongsheng He
Summary: METTL4 is identified as a plant DNA 6mA methyltransferase in Arabidopsis thaliana and plays a crucial role in regulating heat stress response.
Article
Biochemistry & Molecular Biology
Zailong Tian, Kun Li, Yaru Sun, Baojun Chen, Zhaoe Pan, Zhenzhen Wang, Baoyin Pang, Shoupu He, Yuchen Miao, Xiongming Du
Summary: Plants have evolved a mechanism called 'stress memory' to survive in various environmental stresses. This study reveals the physiological, biochemical, and molecular mechanisms underlying drought stress memory formation in cotton, highlighting the role of histone modification H3K4me3 in regulating transcriptional memory. It also investigates the intergenerational inheritance of drought stress memory in cotton, providing theoretical guidance for cotton breeding.