Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 85, Issue -, Pages 9-13Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2014.10.006
Keywords
Nicotiana tabacum; Phyllosphere microorganism; Pyridoxal; Enzymatic reduction; Vitamin B-6
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Funding
- National Natural Science Foundation of China [31372262]
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Vitamin B-6 (VB6) comprises six interconvertible pyridine compounds (vitamers), among which pyridoxal 5'-phosphate (PLP) is a coenzyme involved in a high diversity of biochemical reactions. In plants, PLP is de novo synthesized, and pyridoxine (PN) is usually maintained as the predominant B-6 vitamer. Although the conversion from pyridoxal (PL) to PN catalyzed by PL reductase in plants has been confirmed, the enzyme itself remains largely unknown. We previously found pre-incubation at 35 degrees C dramatically enhanced PL reductase activity in tobacco leaf homogenate. In this study, we demonstrated that the increase in the reductase activity was a consequence of phyllosphere microbial proliferation. VB6 was detected from tobacco phyllosphere, and PL level was the highest among three non-phosphorylated B-6 vitamers. When the sterile tobacco rich in PL were kept in an open, warm and humid environment to promote microorganism proliferation, a significant change from PL to PN was observed. Our results suggest that there may be a plant microbe interaction in the conversion from PL to PN within tobacco phyllosphere. (C) 2014 Elsevier Masson SAS. All rights reserved.
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