Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 70, Issue -, Pages 52-60Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2013.05.015
Keywords
Nucleobase cation symporter 1; Purine; Pyrimidine; Chlamydomonas; Transport
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Funding
- IPFW
- Hatch Fund [CONH00253]
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The single cell alga Chlamydomonas reinhardtii is capable of importing purines as nitrogen sources. An analysis of the annotated C. reinhardtii genome reveals at least three distinct gene families encoding for known nucleobase transporters. In this study the solute transport and binding properties for the lone C. reinhardtii nucleobase cation symporter 1 (CrNCS1) are determined through heterologous expression in Saccharomyces cerevisiae. CrNCS1 acts as a transporter of adenine, guanine, uracil and allantoin, sharing similar - but not identical - solute recognition specificity with the evolutionary distant NCS1 from Arabidopsis thaliana. The results suggest that the solute specificity for plant NCS1 occurred early in plant evolution and are distinct from solute transport specificities of single cell fungal NCS1 proteins. (c) 2013 Elsevier Masson SAS. All rights reserved.
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