Article
Multidisciplinary Sciences
Hsi-En Tsao, Shu Nga Lui, Anthony Hiu-Fung Lo, Shuai Chen, Hiu Yan Wong, Chi-Kin Wong, Liwen Jiang, Kam-Bo Wong
Summary: In Arabidopsis, vacuolar sorting receptor isoform 1 (VSR1) plays a key role in directing 12S globulins and other storage proteins to the protein storage vacuoles during seed development. The crystal structure of VSR1-PA in complex with the C-terminal pentapeptide of cruciferin 1 sheds light on the molecular mechanisms involved in the sorting process. The study provides insights into how VSR1 recognizes and interacts with the C-terminal sorting determinants of various storage proteins, which could potentially aid in targeting recombinant proteins to seed storage vacuoles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Jan Zouhar, Wenhan Cao, Jinbo Shen, Enrique Rojo
Summary: The study of endomembrane trafficking is crucial for understanding cellular and organism functionality. Investigating endomembrane trafficking in plants is of particular interest due to its role in transporting seed storage proteins and secreting cell wall material. While the mechanisms of anterograde transport in plants have been extensively discussed, retrograde trafficking pathways have received less attention. This review summarizes the current understanding of retrograde trafficking in the endomembrane system of plants, discussing its integration with anterograde transport, describing retrieval mechanisms, highlighting contentious issues, and identifying future research questions.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Xin Yong, Lin Zhao, Wenfeng Hu, Qingxiang Sun, Hyoungjun Ham, Zhe Liu, Jie Ren, Zhen Zhang, Yifei Zhou, Qin Yang, Xianming Mo, Junjie Hu, Daniel D. Billadeau, Da Jia
Summary: This study reveals a functional distinction between the FERM domain of SNX27 and SNX17, showing that SNX27 interacts with a novel DLF motif in SNX1/2 instead of the NPxY/NxxY motif in cargo proteins. The interaction between SNX27 and SNX1/2 is critical for efficient recruitment of SNX27 to endosomes and endocytic recycling of multiple cargoes, and is also crucial for brain development in zebrafish. The findings suggest that SNX27 and SNX17 mediate endocytic recycling through fundamentally distinct mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Dana A. Dahhan, Gregory D. Reynolds, Jessica J. Cardenas, Dominique Eeckhout, Alexander Johnson, Klaas Yperman, Walter A. Kaufmann, Nou Vang, Xu Yan, Inhwan Hwang, Antje Heese, Geert De Jaeger, Jiri Friml, Daniel Van Damme, Jianwei Pan, Sebastian Y. Bednarek
Summary: In this study, mass spectrometry was used to analyze the proteome of Arabidopsis suspension-cultured cell clathrin-coated vesicles (CCVs). The study identified evolutionarily conserved and plant-specific factors associated with CCVs. Notably, the AP-4 complex was found to be abundantly present in the CCV proteome.
Article
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiu-Mei Tao, Xiao-Wei Yu
Summary: It was found that knocking out vps10, vps13, and vps21 genes in T. reesei improved cellulase production by alleviating vacuolar transport and degradation. Transcriptome analysis indicated that increased cellulase gene expression might be attributed to the differential expression of sugar transporters. This study provides new clues for higher cellulase production in T. reesei through the alleviation of vacuolar transport via knockout of vps10, vps13, and vps21.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Jian Huang, Jin Gan, Jian Wang, Min Zheng, Han Xiao
Summary: In this study, significant overexpression of 28 VPSs in hepatocellular carcinoma (HCC) tissues compared to normal tissues was observed. The expression of VPSs showed a negative correlation with overall survival, while displaying a positive correlation with tumor grade and stage. VPS72 was identified as a potential independent risk factor for HCC prognosis, and its overexpression was associated with poor prognosis factors and immune cell infiltration levels.
IMMUNITY INFLAMMATION AND DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Jinjin Li, Weiwei Wang, Jinhong Yuan, Jinyu Xu, Lifei He, Xinying Zhang, Haiyan Zhang
Summary: Plants have evolved sophisticated mechanisms to adjust to nutrient deficiency or excess. The COPT2 protein in Arabidopsis thaliana is transcriptionally regulated in response to changing levels of cellular copper, but its activity is subject to degradation in response to excess copper. The protein's stability is affected by proteasome inhibition and its ubiquitination status, highlighting the complexity of plasma membrane transporter degradation mechanisms.
Article
Multidisciplinary Sciences
Hongbo Li, Ruihua Huang, Yanglan Liao, Shuhong Yang, Bojin Feng, Hongting Qin, Jun Zhou, Yonglun Zeng, Jinbo Shen, Xiaohong Zhuang, Liwen Jiang, Marisa S. Otegui, Shengchun Zhang, Caiji Gao
Summary: The study identifies a plant-specific endosomal regulator, BLISTER (BLI), that interacts with the retromer complex and plays a crucial role in regulating endosomal trafficking in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Lu Yang, Weihua Tan, Xinzhi Yang, Yong You, Jing Wang, Gebo Wen, Jing Zhong
Summary: Sorting nexins (SNXs) are a group of diverse cytoplasmic- and membrane-associated phosphoinositide-binding proteins that play a crucial role in regulating intracellular protein trafficking. Dysfunctions of SNX proteins have been linked to various cancerous/neoplastic diseases, making them potential biomarkers and therapeutic targets for cancer diagnostics and treatment.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joao Neves, Miguel Sampaio, Ana Seneca, Susana Pereira, Jose Pissarra, Claudia Pereira
Summary: Plants respond to abiotic stress by remodeling their endomembranes and adapting their trafficking pathways. Differential gene expression related to protein trafficking machinery leads to alterations in cell ultrastructure under stress conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jeong Hun Kim, Hyera Jung, Kyoungjun Song, Han Nim Lee, Taijoon Chung
Summary: Phosphatidylinositol 3-phosphate (PI3P) is a signaling phospholipid that is crucial for endomembrane trafficking, including autophagy and endosomal trafficking. The role of PI3P downstream effectors in plant autophagy, particularly regarding ATG18A and FYVE2, has been unclear. In this study, we demonstrate that FYVE3, a paralog of plant-specific FYVE2, interacts with ATG18A and FYVE2 and contributes to autophagy regulation. FYVE3 is transported to the vacuole and its delivery relies on PI3P biosynthesis and the canonical autophagic machinery. The fyve3 mutation suppresses defective autophagy in fyve2 mutants, suggesting a specific role of FYVE3 in FYVE2-dependent autophagy.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jing Lu, Suowen Xu, Yuqing Huo, Duanping Sun, Yuehuai Hut, Junjian Wang, Xiaolei Zhang, Panxia Wang, Zhuoming Li, Mengya Liang, Zhongkai Wu, Peiqing Liu
Summary: The study reveals that SNX3 plays a key role in cardiac function and is crucial for myocardial injury, suggesting that SNX3 could be a potential therapeutic target for cardiac hypertrophy and heart failure.
CELL DEATH AND DIFFERENTIATION
(2021)
Review
Biology
D. C. Ghislaine Mayer
Summary: This review discusses the latest findings on the molecular mechanisms of protein sorting and vesicular trafficking in P. falciparum, showing that the parasite has repurposed molecules of the endosomal system for its secretory pathway. The sorting and vesicular trafficking mechanism appears to be conserved among apicomplexans, indicating a clever modification of P. falciparum's secretory machinery for its intracellular lifestyle.
Article
Immunology
Tuan-yuan Shi, Tian-en Li, Yun Hao, Hong-chao Sun, Yuan Fu, Wen-chao Yan, Li-li Hao
Summary: In this study, a partial sequence of Eimeria tenella VPS29 (EtVPS29) was cloned and expressed. It was found that EtVPS29 was widely distributed in the cytoplasm of E. tenella sporozoites and showed a protective effect against E. tenella infection in chickens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Lei Yang, Liwen Jiang
Summary: Vacuolar sorting is crucial for plants and plays a role in important agricultural traits. This study focused on the subcellular localization of seven vacuolar sorting receptors (VSRs) in rice and confirmed their role as markers for the prevacuolar compartment/multivesicular body (PVC/MVB) in rice.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
