Article
Plant Sciences
Matthew E. Dwyer, Roger P. Hangarter
Summary: This study provides insights into the mechanistic role of THRUMIN1 in light-dependent chloroplast movements, including its interactions with actin filaments, the impact of phosphorylation sites, association with 14-3-3 proteins, and the involvement of THRUMIN1's C-terminus in chloroplast movement.
Article
Chemistry, Multidisciplinary
Yun-Wei Xue, Kensuke Miura, Hiroaki Itoh, Masayuki Inoue
Summary: Polytheonamide B (1) is a large peptide that forms a transmembrane ion channel and exhibits potent cytotoxicity against MCF-7 cancer cells. The functions of depolarizing the plasma membrane and neutralizing acidic lysosomes have been uncoupled by designing and synthesizing new analogues of 1.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biology
Kazusato Oikawa, Takuto Imai, Chonprakun Thagun, Kiminori Toyooka, Takeshi Yoshizumi, Kazuya Ishikawa, Yutaka Kodama, Keiji Numata
Summary: The researchers investigated the rate and trajectory of mitochondria in Arabidopsis thaliana mesophyll cells using confocal laser-scanning microscopy. They discovered that mitochondria migrate through wiggling during their association with chloroplasts, shedding light on the impact of mitochondria-chloroplast interaction on mitochondrial movement.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Roa'a Jaradat, Xiaole Li, Honghong Chen, Peter B. Stathopulos, Donglin Bai
Summary: Functional studies and structural models of Cx46 and Cx50 GJs reveal the importance of hydrophobic interactions between the NT and TM2 domains in V-j-gating, gamma(j), and channel open stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chao-Hua Jiang, Mengping Wei, Chen Zhang, Yun Stone Shi
Summary: Long-term potentiation (LTP) is a crucial cellular mechanism for learning and memory, with the amino-terminal domain (ATD) of GluA1 interacting with neuroplastin-65 (Np65) playing a key role in anchoring AMPARs at the postsynaptic membrane during LTP.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Shota Kato, Yamato Takahashi, Yuta Fujii, Kotoko Sasaki, Satoyuki Hirano, Koji Okajima, Yutaka Kodama
Summary: This study investigates the functional differences of the photoreceptor Phototropin (phot) in plants, specifically focusing on the light-oxygen-voltage (LOV) domains in species with duplication events and those without. The research reveals that even in unduplicated liverworts, Mpphot exhibits some similar functions to duplicated photosensitive proteins, highlighting conservation and diversification among LOV domains in the evolution of land plants.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Michael Reidy, Kevin Garzillo, Daniel C. Masison
Summary: Hsp90 is an essential eukaryotic chaperone that regulates protein activity. The Hsp82-E33A mutant, which binds ATP but does not hydrolyze it, supports viability of S. cerevisiae and other eukaryotic species, suggesting that ATP hydrolysis is not necessary for Hsp90 function. The exchange of ATP for ADP is critical for Hsp90 function, with ATP hydrolysis providing an important control point regulated by co-chaperones.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Guanlan Fan, Fan Wang, Yurou Chen, Qian Zheng, Jie Xiong, Qiongying Lv, Kejia Wu, Jiaqiang Xiong, Lei Wei, Dongqing Li, Jiachen Zhang, Wei Zhang, Feng Li
Summary: In this study, OTUD1 is identified as an Akt-associated protein and is downregulated upon Akt activation. Ectopic OTUD1 inhibits Akt phosphorylation, and a short peptide (OUN-36) located in the OTUD1 N-terminal intrinsically disordered region strongly binds to the Akt PH domain. OUN-36-based therapy efficiently abrogates Akt feedback reactivation and sensitizes cancer cells to chemotherapy and immunotherapy.
Article
Virology
Chelsea T. Barrett, Hadley E. Neal, Kearstin Edmonds, J. Lizbeth Reyes Zamora, Carole L. Moncman, Andreea Popa, Everett Clinton Smith, Stacy R. Webb, Rebecca Ellis Dutch
Summary: Hendra virus uses two surface glycoproteins to infect host cells, with the TM region of the F protein playing a key role in stability and fusion. Mutations at residues M491 and L492 have been shown to alter F protein function, indicating their involvement in the fusion process.
Article
Biochemistry & Molecular Biology
Natalia Ruiz -Molina, Juliana Parsons, Eva L. Decker, Ralf Reski
Summary: Human complement is crucial for defense against pathogens and tissue homeostasis. Factor H (FH) and Factor H-related protein 1 (FHR1) are important complement regulators. The structure of synthetic regulators based on FH and FHR1 were modeled using AlphaFold2, and the interactions between C3 fragments, MAC components (C5, C7, and C9), and various regulators were studied using AlphaFold-Multimer (AFM). The findings provide insights into the molecular mechanisms of complement regulation and can guide the development of complement-based therapies.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ravichandran Vignesh, Anita Sjolander, Ganesh Venkatraman, Suresh Kumar Rayala, Gopala Krishna Aradhyam
Summary: The characteristic features of cancer cells include aberrant intracellular pH and elevated phosphatidylserine levels. Research focuses on discovering biomarkers for early diagnosis and therapy. Calnuc, expressed in azoxymethane-treated mice and contained in tumor cell exosomes, drives epithelial-mesenchymal transition and migration in non-tumor cells, potentially leading to metastasis.
BIOCHEMICAL JOURNAL
(2021)
Article
Plant Sciences
Xiaohui Zhao, Zhengqiang Chen, Qian Wu, Yazhen Cai, Yu Zhang, Ruizhen Zhao, Jiaoling Yan, Xin Qian, Jia Li, Min Zhu, Lizhou Hong, Jincheng Xing, Nasr Ullah Khan, Yinghua Ji, Peijun Wu, Changjun Huang, Xin Shun Ding, Hui Zhang, Xiaorong Tao
Summary: Studies have shown that the nucleotide-binding domain Sw-5b NLR alone can activate downstream defense signaling in tomato, leading to elicitor-independent cell death. Self-association of the Sw-5b NB domain is crucial for activating cell death signaling, while mutations can compromise this function. These findings suggest a potential mechanism for the activation of defense signaling in plant cells.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Chemistry, Multidisciplinary
Camila A. Cotrim, Russell J. Jarrott, Andrew E. Whitten, Hassanul G. Choudhury, David Drew, Jennifer L. Martin
Summary: In this study, it was demonstrated that human ZnT1 and its variant can be expressed and purified in yeast cells, with the purified hZnT1 variant showing Zn2+/H+ antiporter activity. Additionally, the soluble C-terminal domain of hZnT1 was successfully expressed, purified, and characterized in a bacterial expression system, showing an increase in melting temperature at acidic pH and forming a dimer in solution based on small-angle X-ray scattering analysis.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Emilie Aponte, Marie Lafitte, Audrey Sirvent, Valerie Simon, Maud Barbery, Elise Fourgous, Mariano Maffei, Florence Armand, Romain Hamelin, Julie Pannequin, Philippe Fort, Miquel Pons, Serge Roche, Yvan Boublik
Summary: This study reveals the important role of the unique domain ULBR in Src tyrosine kinase in malignant cell transformation. The ULBR is involved in membrane anchoring, MAPK signaling, and phosphorylation of specific membrane-localized tyrosine kinases needed for Src oncogenic signaling.
Article
Cell Biology
Hidenori Ito, Koh-ichi Nagata
Summary: CNKSR2 is a scaffolding molecule that interacts with various molecules, playing a role in signal transduction and regulating neuronal development. It is considered as a causative gene for X-linked intellectual disability.
Article
Biochemistry & Molecular Biology
Yang-Tsung Lin, Lih-Jen Chen, Cornelia Herrfurth, Ivo Feussner, Hsou-min Li
Article
Plant Sciences
Po-Kai Huang, Po-Ting Chan, Pai-Hsiang Su, Lih-Jen Chen, Hsou-min Li
Article
Plant Sciences
Chiung-Chih Chu, Hsou-min Li
FRONTIERS IN PLANT SCIENCE
(2015)
Article
Plant Sciences
Yih-Lin Chen, Lih-Jen Chen, Hsou-min Li
Article
Plant Sciences
Lih-Jen Chen, Hsou-Min Li
Article
Plant Sciences
Jun-Shian Chang, Lih-Jen Chen, Yi-Hung Yeh, Chwan-Deng Hsiao, Hsou-min Li
Review
Plant Sciences
Chiung-Chih Chu, Hsou-min Li
PHOTOSYNTHESIS RESEARCH
(2018)
Review
Plant Sciences
Hsou-min Li, Chun-Wei Yu
PLANT AND CELL PHYSIOLOGY
(2018)
Review
Plant Sciences
Hsou-min Li, Yi-Shan Teng
TRENDS IN PLANT SCIENCE
(2013)
Article
Multidisciplinary Sciences
Yih-Lin Chen, Lih-Jen Chen, Chiung-Chih Chu, Po-Kai Huang, Jie-Ru Wen, Hsou-min Li
Article
Biochemistry & Molecular Biology
Chiung-Chih Chu, Krishna Swamy, Hsou-min Li
Letter
Biochemistry & Molecular Biology
Hsou-min Li, Danny Schnell, Steven M. Theg
Article
Plant Sciences
Chun-Wei Yu, Yang-Tsung Lin, Hsou-min Li
Article
Plant Sciences
Meng-Rong Chuang, Lih-Jen Chen, Hsou-min Li
Summary: Chloroplasts are divided into six subcompartments, and Tic22 is one of the proteins identified as localizing to the intermembrane space. Research suggests that Tic22 may use part of the general translocon for import into the intermembrane space.
Article
Multidisciplinary Sciences
Jun Fang, Bingqi Li, Lih-Jen Chen, Vivek Dogra, Shengji Luo, Wangpin Wu, Pengcheng Wang, Inhwan Hwang, Hsou-Min Li, Chanhong Kim
Summary: This study reveals a related component of TIC236, CRL, which is involved in plastid division and plant stress responses. The findings highlight the interconnectedness between plastid protein import, plastid division, and plant stress responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)