Article
Multidisciplinary Sciences
Hsi-En Tsao, Shu Nga Lui, Anthony Hiu-Fung Lo, Shuai Chen, Hiu Yan Wong, Chi-Kin Wong, Liwen Jiang, Kam-Bo Wong
Summary: In Arabidopsis, vacuolar sorting receptor isoform 1 (VSR1) plays a key role in directing 12S globulins and other storage proteins to the protein storage vacuoles during seed development. The crystal structure of VSR1-PA in complex with the C-terminal pentapeptide of cruciferin 1 sheds light on the molecular mechanisms involved in the sorting process. The study provides insights into how VSR1 recognizes and interacts with the C-terminal sorting determinants of various storage proteins, which could potentially aid in targeting recombinant proteins to seed storage vacuoles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Lei Yang, Liwen Jiang
Summary: Vacuolar sorting is crucial for plants and plays a role in important agricultural traits. This study focused on the subcellular localization of seven vacuolar sorting receptors (VSRs) in rice and confirmed their role as markers for the prevacuolar compartment/multivesicular body (PVC/MVB) in rice.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eleanor L. Mitchell, Franco Viscarra, Isabel Bermudez, Joseph Hawkins, Jim A. Goodchild, Andrew K. Jones
Summary: This study presents the functional expression of the alpha 5 nAChR subunit from honey bees in a heterologous system, revealing its low sensitivity to acetylcholine and the efficacy of serotonin as an agonist. Molecular modelling suggests that residues in the binding site may play a role in the receptor's selectivity towards serotonin. This finding may provide potential targets for the development of novel insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Cell Biology
Jan Zouhar, Wenhan Cao, Jinbo Shen, Enrique Rojo
Summary: The study of endomembrane trafficking is crucial for understanding cellular and organism functionality. Investigating endomembrane trafficking in plants is of particular interest due to its role in transporting seed storage proteins and secreting cell wall material. While the mechanisms of anterograde transport in plants have been extensively discussed, retrograde trafficking pathways have received less attention. This review summarizes the current understanding of retrograde trafficking in the endomembrane system of plants, discussing its integration with anterograde transport, describing retrieval mechanisms, highlighting contentious issues, and identifying future research questions.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Cerrone Cabanos, Yuki Matsuoka, Nobuyuki Maruyama
Summary: This review article focuses on the major seed storage proteins of soybean, their three-dimensional structures, nutritional importance, and bioactive peptides. It also summarizes past efforts in the recombinant production of foreign proteins or bioactive peptides in soybean seed.
Article
Biochemical Research Methods
Yasen Wang, Buqing Wang, Yahui Gao, Hideki Nakanishi, Xiao-Dong Gao, Zijie Li
Summary: This study achieved highly efficient expression and secretion of human lysozyme (hLYZ) in Pichia pastoris using multiple strategies. The highest hLYZ activity reached 352,000 U/mL in a 5L fermenter. This research provides a cost-effective approach for hLYZ production and can be applied to the production of other recombinant proteins.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Virology
Nadishka Jayawardena, Linde A. Miles, Laura N. Burga, Charles Rudin, Matthias Wolf, John T. Poirier, Mihnea Bostina
Summary: Research shows that N-linked glycosylation in ANTXR1 is necessary for SVV attachment and entry, and could be a crucial factor for patient selection in future cancer therapy. Experimental validation indicates the essential role of this glycosylation in establishing stable interactions between SVV and ANTXR1.
Article
Cell Biology
Sebastian Eising, Bianca Esch, Mike Waelte, Prado Vargas Duarte, Stefan Walter, Christian Ungermann, Maria Bohnert, Florian Froehlich
Summary: Lysosome is a crucial catabolic organelle in cells, with mutations in lysosomal proteins potentially leading to neurodegeneration, cancer, and age-related diseases. The vacuole serves as a lysosomal analog in Saccharomyces cerevisiae, containing evolutionarily conserved proteins. Proteins can reach vacuoles through different pathways, including the Vps10-dependent endosomal vacuolar protein sorting pathway, the alkaline phosphatase pathway, and the cytosol-to-vacuole transport pathway.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Immunology
Jian Huang, Jin Gan, Jian Wang, Min Zheng, Han Xiao
Summary: In this study, significant overexpression of 28 VPSs in hepatocellular carcinoma (HCC) tissues compared to normal tissues was observed. The expression of VPSs showed a negative correlation with overall survival, while displaying a positive correlation with tumor grade and stage. VPS72 was identified as a potential independent risk factor for HCC prognosis, and its overexpression was associated with poor prognosis factors and immune cell infiltration levels.
IMMUNITY INFLAMMATION AND DISEASE
(2023)
Article
Biology
Astrid S. Jorgensen, Viktorija Daugvilaite, Katia De Filippo, Christian Berg, Masa Mavri, Tau Benned-Jensen, Goda Juzenaite, Gertrud Hjorto, Sara Rankin, Jon Vabeno, Mette M. Rosenkilde
Summary: Plerixafor exhibits biased action by reversing the CXCL12 gradient across the bone marrow endothelium and showing superior therapeutic effect in HSC mobilization. In contrast, AMD11070, despite having superior antagonism, does not perform as effectively in HSC mobilization in vivo.
COMMUNICATIONS BIOLOGY
(2021)
Article
Agronomy
Xuekai Shi, Xiaojian Liu, Anastasia Mw Cooper, Kristopher Silver, Hans Merzendorfer, Kun Yan Zhu, Jianzhen Zhang
Summary: This study identified and characterized the V-ATPase-c gene in Locusta migratoria and found that it is primarily expressed in the Malpighian tubules. The silencing of LmV-ATPase-c resulted in severe molting defects and impaired RNA interference efficiency in the insect. These findings suggest that LmV-ATPase-c plays a crucial role in cuticle formation and dsRNA escape.
PEST MANAGEMENT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ryu Imamura, Hiroki Sato, Dominic Chih- Cheng Voon, Takayoshi Shirasaki, Masao Honda, Makoto Kurachi, Katsuya Sakai, Kunio Matsumoto
Summary: Met in epithelial tissue plays a crucial role in cytokine production, and its tyrosine kinase activity is not required for this process. The interaction between the intracellular carboxy terminus of Met and MAVS in RLR-mediated signaling promotes immune responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Jini Sugatha, Amulya Priya, Prateek Raj, Ebsy Jaimon, Uma Swaminathan, Anju Jose, Thomas John Pucadyil, Sunando Datta, Felix Campelo
Summary: SNX32 interacts with SNX4 via its BAR domain and with TfR, CIMPR, and BSG through its PX domain, regulating cargo sorting and trafficking processes. Silencing of SNX32 leads to defects in intracellular trafficking and neuronal differentiation, suggesting its crucial role in maintaining neuroglial coordination.
Article
Immunology
Kun Yang, Jie Han, Jennifer G. Gill, Jason Y. Park, Meghana N. Sathe, Jyothsna Gattineni, Tracey Wright, Christian Wysocki, M. Teresa de la Morena, Nan Yan
Summary: Mutations in the SKIV2L gene cause severe B cell immunodeficiency in THES patients, indicating its crucial role in early B cell development.
SCIENCE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Evgenia Fadzeyeva, Cassandra A. A. Locatelli, Natasha A. Trzaskalski, My-Anh Nguyen, Megan E. Capozzi, Branka Vulesevic, Nadya M. Morrow, Peyman Ghorbani, Antonio A. Hanson, Ilka Lorenzen-Schmidt, Mary-Anne Doyle, Richard Seymour, Elodie M. Varin, Morgan D. Fullerton, Jonathan E. Campbell, Erin E. Mulvihill
Summary: Mice lacking DPP4 have improved islet health, glucoregulation, and reduced obesity with HFD feeding. The improvement can be partly attributed to the loss of DPP4 in non-EC types. Beta cell incretin receptors are necessary for DPP4 inhibitor effects on insulin secretion and glucose tolerance in HFD-fed mice.
Article
Biochemistry & Molecular Biology
Zhiming Ma, Yanbiao Sun, Xinlu Zhu, Liang Yang, Xu Chen, Yansong Miao
Summary: This study demonstrates that the condensation of nanodomain constituents and the self-assembly of remorin proteins regulate the condensation and activity of formins during innate immunity in Arabidopsis thaliana. Through interaction with formins, remorin enhances actin nucleation, influencing the transduction of immune signals. Such nanodomain- and remorin-mediated regulation is expected to be a common feature in plant innate immune responses, creating spatially separated biochemical compartments and fine-tuning membrane properties for immune signal transduction in the host.
Article
Materials Science, Biomaterials
Young Eun Lee, Chae Min Yuk, Minseok Lee, Ki-Cheol Han, Eunsung Jun, Tae Sung Kim, Ja-Lok Ku, Sung G. Im, Eunjung Lee, Mihue Jang
Summary: This study introduces a novel 3D assay platform for monitoring NK-mediated cancer cell killing, offering a new approach for studying cancer therapy.
BIOMATERIALS SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Qiuyu Gong, Lei Chen, Jining Wang, Fenghou Yuan, Zhiming Ma, Guoxin Chen, Yinjuan Huang, Yansong Miao, Tian Liu, Xin-Xing Zhang, Qing Yang, Jing Yu
Summary: This study reports that a highly abundant cuticular protein OfCPH-1 from the Asian corn borer has the ability to form gel-like droplets in the presence of chitosan. The transition from liquid-like to gel-like state is driven by hydrogen-bonding interactions and is associated with the induced beta-sheet structure of OfCPH-1. This liquid-liquid phase separation process and its aging behavior may play critical roles in the formation of the insect cuticle.
Review
Biochemistry & Molecular Biology
Kexin Guo, Changjin Huang, Yansong Miao, Daniel J. Cosgrove, K. Jimmy Hsia
Summary: Plants produce a wide variety of leaf forms, and the mechanisms of leaf morphogenesis have been extensively studied with a recent focus on the interactions between mechanics and morphology. Mechanical stress plays a crucial role in the growth and development of plant organs, but the underlying principles of morphogenesis guided by mechanics are not well understood.
Article
Biochemistry & Molecular Biology
Zhiming Ma, Kexin Zhu, Yong-Gui Gao, Suet-Mien Tan, Yansong Miao
Summary: The actin cytoskeleton undergoes rapid remodelling during signal transduction, and the plant class I formin family plays a major role in this process. The PM-integrated formins are highly responsive to mechanical perturbation, regulating their condensation on the cell surface. However, the molecular mechanisms underlying the mechanosensing and mechanoregulation of formin in actin remodelling remain unclear.
Review
Biochemistry & Molecular Biology
Ya-Wen He, Yinyue Deng, Yansong Miao, Subhadeep Chatterjee, Tuan Minh Tran, Jing Tian, Steven Lindow
Summary: While bacteria are unicellular, they have the ability to communicate with each other and their environment for adaptation. Quorum sensing is a well-researched mode of cell-to-cell communication, extending beyond bacterial communication to communication between bacteria and their eukaryotic hosts. This review focuses on the diffusible signal factor (DSF) family, which plays a crucial role in communication among diverse Gram-negative bacteria, fungi, insects, plants, and zebrafish.
TRENDS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zhitong Zhao, Tao Xu, Xiaoyong Pan, Susanti, Jason C. White, Xiao Hu, Yansong Miao, Philip Demokritou, Kee Woei Ng
Summary: This study explores the potential of upcycling and repurposing keratinous and cellulosic wastes to provide sustainable nutrient delivery to crops. The researchers fabricated composite substrates using keratin derived from human hair and cellulose nanofibers obtained from wood pulp. These substrates exhibited excellent physical properties and hydrophilicity, and were able to support seed germination and seedling development through controlled release of nutrients.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Editorial Material
Plant Sciences
Ruixi Li, Georgia Drakakaki, Yansong Miao, Ying Fu, Xiaojuan Li
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
HaiYan Wang, Jinrong Hu, Kexi Yi, Zhiming Ma, XinJie Song, Yaelim Lee, Petr Kalab, Alexander D. Bershadsky, Yansong Miao, Rong Li
Summary: In this study, the recruitment of FMN2 to the ER around the spindle and its activity regulation in mouse meiosis I oocytes were investigated. The results showed that this process is regulated by the Ran GTPase and the ER-associated protein VAPA. The binding of FMN2 to importin a1/b complex and its subsequent disruption by RanGTP were required for FMN2 accumulation near the chromatin and the MI spindle. The importin-free FMN2 was then recruited to the ER surface around the spindle through its interaction with VAPA. Moreover, autoinhibition of FMN2 through an intramolecular interaction between its SLD and FH2 domain was relieved by the binding of VAPA, leading to localized actin polymerization. This study sheds light on the crucial role of FMN2 in the maturation of mammalian female gametes.
Article
Nanoscience & Nanotechnology
Nicolo Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. . Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marite Cardenas
Summary: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We investigated the structure of lipid bilayers formed on nanoparticle arrays through vesicle fusion using scattering techniques and direct imaging. The presence of both flat and curved supported lipid bilayers on the same substrate, combined with the accuracy of grazing incidence neutron scattering, offers a promising approach to studying curvature-dependent membrane phenomena.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell Biology
Xiao Han, Zixin Hu, Wahyu Surya, Qianqian Ma, Feng Zhou, Lars Nordenskiold, Jaume Torres, Lanyuan Lu, Yansong Miao
Summary: The IDR region in the coronin family plays a critical role in optimizing the biochemical activities of coronins, both in vivo and in vitro, by fine-tuning oligomerization and maintaining proper protein structure.
Article
Oncology
Young Eun Lee, Ga-Yeon Go, Eun-Young Koh, Han-Na Yoon, Minkoo Seo, Seung-Mo Hong, Ji Hye Jeong, Jin-Chul Kim, Duck Cho, Tae Sung Kim, Song Cheol Kim, Eunsung Jun, Mihue Jang
Summary: Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) impair the functionality of natural killer (NK) cells, which are considered as a promising therapeutic modality. Targeted therapies against CAFs may enhance NK-mediated cancer killing.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Review
Plant Sciences
Yansong Miao, Xiangfu Guo, Kexin Zhu, Wenting Zhao
Summary: This review discusses the diverse spatiotemporal regulation of cellular signal transduction by membraneless organelles through recruiting necessary signaling factors. Specifically, it focuses on the formation of multicomponent immune signaling hubs at the plasma membrane interface during host-pathogen interactions, and the importance of macromolecular condensation in regulating immune signaling outputs and crosstalk between signaling pathways.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Plant Sciences
Xiaolin Liu, Zhiming Ma, Tuan Minh Tran, Carsten Rautengarten, Yingying Cheng, Liang Yang, Berit Ebert, Staffan Persson, Yansong Miao
Summary: The plant cell wall is an important physical barrier for phytopathogens to invade their hosts, and it responds to pathogen infection through a complex network of immune receptors. This study found a negative correlation between cellulose and callose biosynthesis in Arabidopsis, and this balance is mediated by the activity of a beta-1,3-glucanase.
Article
Plant Sciences
Bin Yu, Xuemei He, Yang Tang, Zhonghui Chen, Limeng Zhou, Xiaoming Li, Chunyu Zhang, Xiang Huang, Yuhua Yang, Wenbin Zhang, Fanjiang Kong, Yansong Miao, Xingliang Hou, Yilong Hu
Summary: Photoperiod plays a prominent role in seed development by controlling seed size in different plant species. The central regulator CONSTANS directly represses the transcription of AP2, thereby modulating seed size in a photoperiod-dependent manner.
