Article
Plant Sciences
Felix Moran, Mario Herrero-Cervera, Sofia Carvajal-Rojas, Ester Marco-Noales
Summary: Huanglongbing (HLB) is a devastating disease that affects all commercial citrus species worldwide, and it is transmitted by psyllid vectors. This study presents a new real-time recombinase polymerase amplification (RPA) protocol that can detect the three bacteria species associated with HLB in both plant and insect samples. The protocol is accurate, sensitive, specific, and reliable, and it does not require specialized equipment, making it ideal for field use. The results indicate the potential of this new RPA protocol to be implemented as a reliable on-site detection kit for HLB.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Engineering, Environmental
Aron Mari, Gabor Bordos, Szilveszter Gergely, Monika Buki, Judit Hahn, Zoltan Palotai, Gabriella Besenyo, Eva Szabo, Andras Salgo, Balazs Kriszt, Sandor Szoboszlay
Summary: Studies show the potential risks of microplastics to ecosystems and the need for standardized methods for measuring their concentration. A new study has developed a Small Volume Glass Separator for enhanced microplastic recovery, showing higher recovery rates than traditional methods and effectiveness across various microplastic types.
Article
Biochemistry & Molecular Biology
Elena Fabris, Michela Bulfoni, Alessandro Nencioni, Emanuele Nencioni
Summary: This study illustrated the development and validation of a spectrophotometric enzymatic assay for alpha-Gal activity quantification in dietary supplements. The method showed good performance in quantitatively determining alpha-Gal activity in commercial food supplements according to ICH guidelines, and is suitable for rapid in-house quality control testing.
Article
Engineering, Environmental
S. M. Mohammed Al-Azzawi, Oliver Knoop, E. Jorg Drewes
Summary: The interest in monitoring microplastics in the environment has grown, but the challenge lies in the interactions with complex environmental matrices, especially wastewater effluents rich in natural organic matter (NOM). Sample preparation methods are needed to remove NOM and minimize interference during analysis. This study successfully tagged and fractured microplastic particles to assess the effects of sample preparation methods. The results suggest that both Fenton and hydrogen peroxide methods can be used for small microplastic particles (1-10 μm), while hydrogen peroxide is more suitable for nanoplastics (<1 μm).
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Construction & Building Technology
Josef Kaufmann, Roman Loser, Frank Winnefeld, Andreas Leemann
Summary: Sulfate-resisting cement is recommended for shotcrete in contact with sulfate-containing groundwater, but it may not guarantee sufficient durability. Testing sulfate resistance in accelerated tests is necessary, and laboratory-produced samples showed similar properties to on-site shotcrete in terms of sulfate resistance, porosity, transport properties, and chemical phase assemblage.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Food Science & Technology
Emmanuel K. Tangni, Julien Masquelier, Els Van Hoeck
Summary: Apple and apple derivatives are the most contaminated foodstuffs with patulin, and a method using LC-MS/MS was developed to monitor and ensure the patulin levels are below the maximum permitted levels. The method was validated and applied to juices, purees, and ciders purchased in Belgium, showing the presence of patulin in a significant percentage of apple juices and a small percentage of puree samples. The results highlight the need for regular surveillance of the quality of apple juices and purees in the market.
Article
Infectious Diseases
Faraj Hijaz, Yasser Nehela, Ozgur Batuman, Nabil Killiny
Summary: The study investigated the use of citrate for detecting oxytetracycline in citrus tissues, revealing that plant matrix reduced fluorescence intensity of the europium complex and accelerated phenol degradation. By cleaning sample extracts with solid-phase extraction, successful measurement of OTC levels was achieved.
Article
Chemistry, Analytical
Victoria Silva Amador, Juliane Soares Moreira, Rodinei Augusti, Ricardo Mathias Orlando, Evandro Piccin
Summary: This paper introduces a novel method combining a four-phase electroextraction system with paper spray mass spectrometry for the extraction, pre-concentration, and direct analysis of target compounds in different samples. The proposed method improves protocol efficiency, reduces analysis time, and enhances sensitivity. The feasibility of the method was validated through analysis of biofluid, food, and water quality samples.
Review
Biochemical Research Methods
Ryan Zenhausern, Chia-Hung Chen, Jeong-Yeol Yoon
Summary: The techniques for preparing clinical samples have been perfected for diagnostic testing, but microfluidics has emerged as a popular method for rapid virus detection in resource-limited environments. Early microfluidic prototypes faced limitations due to reliance on off-chip preprocessing steps and external equipment, but recent advancements have allowed for all-in-one, automated virus detection with no need for specialized training or complex machinery. These devices can operate independently and efficiently to provide rapid, automated sample preparation and efficient detection of viral samples.
Article
Biochemical Research Methods
Alexandra Perebikovsky, Yujia Liu, Alexander Hwu, Horacio Kido, Ehsan Shamloo, Dian Song, Gabriel Monti, Oren Shoval, Daniel Gussin, Marc Madou
Summary: This study demonstrates a novel sample incubation technique on a microfluidic centrifugal disc, showing enhanced incubation for E. coli isolates and successful identification of antibiotic resistance within under 2 hours.
Review
Chemistry, Analytical
Fei Xu, Jiedan Zhou, Hai Yang, Linzhou Chen, Jinjian Zhong, Yihong Peng, Ke Wu, Yukai Wang, Huajun Fan, Xiangliang Yang Yuliang Zhao
Summary: This review focuses on the contributions of exhaled breath (EB) preparation in drug of abuse (DOA) detection. The use of available EB sampling devices and related sample pretreatment methods coupled with chromatographic approaches has enabled the detection of DOAs. Various EB sampling devices and sample pretreatment methods are detailed, with an emphasis on the use of adsorbents to improve the concentration and enrichment of target analytes. The applications of EB analysis in different types of DOAs are summarized and commented, and the challenges and future perspectives in sampling devices and sample pretreatment techniques for DOA detection are discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, Legal
Guang-Yao Fan, Dan-Ping Wang, Dan-Lu Song, Xing-Kai Zheng, Jing Zhu, Bing Long
Summary: The STRtyper-32G PCR Amplification Kit is a multiplex system that combines 30 autosomal STR loci, an Indel site, and the sex-determinant locus Amelogenin. It has been validated following SWGDAM guidelines and proven to be a robust tool for forensic database applications. This novel system meets the compatibility requirements for databasing and has been optimized for DNA amplification on different substrates.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Horticulture
Diogo Felix-Oliveira, Jose Alberto Pereira, Jacinto Benhadi-Marin
Summary: The citrus greening disease is caused by Candidatus Liberibacter spp. The African citrus psyllid is a vector of the disease, but spiders may act as natural enemies of the psyllid. Experimental results show that different web architecture significantly affects the trapping efficiency of the spiders.
Article
Biochemical Research Methods
Morteza Alipanah, Carlos Manzanas, Xin Hai, John A. Lednicky, Alberto Paniz-Mondolfi, J. Glenn Morris, Z. Hugh Fan
Summary: To differentiate Mayaro virus (MAYV) from other similar viruses diagnostically, a portable device integrating sample preparation with real-time, reverse-transcription, loop-mediated isothermal amplification (rRT-LAMP) has been developed. The device successfully detected MAYV without cross-reactions with CHIKV, DENV, or ZIKV. The platform allows rapid detection of MAYV in as short as 13 minutes, with a limit of detection as low as 10 genome equivalents per reaction.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Marine
Savin Viswanathan, Christian Holden, Olav Egeland, Ronny Sten
Summary: In this paper, the authors extend the cosimulation methodology to a full scale riser-tensioner system, using OrcaFlex to model the semi-submersible platform and the riser stack, and SimulationX to model the hydro-pneumatic tensioner. The two models are coupled using a Python interface file. Field measurements from an operational semi-submersible drilling platform are used to validate the model. A planned disconnect sequence is simulated and compared with field measurements. Sample simulations are also presented to demonstrate the capabilities of the proposed co-simulation methodology.
Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Everton de Carvalho, Eduardo A. Girardi, Silvio A. Lopes
Summary: Las, associated with HLB, moves laterally through phloem in plants, predominantly towards newly developing root and leaf tissues. Protecting rapidly growing shoots from feeding by D. citri is crucial to minimize transmission and spread of the pathogen.
Article
Agronomy
Felix Moran, Silvia Barbe, Saskia Bastin, Inmaculada Navarro, Edson Bertolini, Maria M. Lopez, Estrella Hernandez-Suarez, Alberto Urbaneja, Alejandro Tena, Felipe Siverio, Ester Marco-Noales
Summary: HLB, caused by three species of 'Ca. Liberibacter', is detected early using PCR, but detecting phytopathogenic bacteria in various environmental samples can be problematic. A study showed that most amplifications in various samples were associated with parasitic wasps, suggesting the need for improved PCR methods to avoid false alarms.
Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Nik J. Cunniffe, Robert Turgeon, Silvio A. Lopes
Summary: The study found that the movement speed of Las in plants is between 2.9-3.8 cm per day, and the colonization of plants by Las is influenced by temperature, with 25.7 degrees Celsius being the optimal condition. This explains the higher impact and incidence of huanglongbing disease during the winter months or regions of milder climates in Brazil.
Article
Plant Sciences
Silvio A. Lopes, Juan C. Cifuentes-Arenas
Summary: A new protocol for transmitting the huanglongbing (HLB) pathogen and an index for favorability to infection (IFI) were proposed, providing alternative ways for growers to control the Asian citrus psyllid (ACP) and assess orchard vulnerabilities to 'Ca. Liberibacter asiaticus'.
Article
Agronomy
Rafael Oliveira Moreira, Eduardo de Andrade Bressan, Horst Bremer Neto, Angelo Pedro Jacomino, Antonio Figueira, Francisco de Assis Alves Mourao Filho
Summary: The native Brazilian fruit crop, cambuci, has great potential as a new fruit crop. Genetic diversity analysis using microsatellite markers revealed that the genetic diversity of cambuci is mainly within populations, with low genetic diversity among populations. A core collection with high genetic diversity was selected using Darwin analysis.
GENETIC RESOURCES AND CROP EVOLUTION
(2022)
Article
Virology
Elena G. Biosca, Jose Francisco Catala-Senent, Angela Figas-Segura, Edson Bertolini, Maria M. Lopez, Belen alvarez
Summary: The genomic sequences of three lytic bacteriophages against Ralstonia solanacearum were analyzed, revealing that they belong to the same novel species within the Gyeongsanvirus family. These phages have the potential for biological control of R. solanacearum.
Article
Agronomy
Priscila Ayumi Sybuia, Gabriela de Castro-Prado, William Mario de Carvalho Nunes, Carlos Alexandre Zanutto, Edilson Nobuyoshi Kaneshima, Dartanha Jose Soares, Claudineia Conationi da Silva Franco, Paulo Cezar de Freitas Mathias, Marialba Avezum Alves de Castro-Prado
Summary: This study evaluates the diversity, genetic variability, and occurrence of parasexual recombination in Brazilian isolates of the economically important fungal pathogens M. pseudophaseolina and M. euphorbiicola. The results suggest that these species have genetic variability and parasexual recombination may be the cause for the origin of new pathotypes.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Review
Microbiology
Cecilia Tamborindeguy, Fernando Teruhiko Hata, Rubia de Oliveira Molina, William Mario de Carvalho Nunes
Summary: This review focuses on the co-transmission of plant pathogens by insects, highlighting key findings and challenges in studying this phenomenon. The review examines the competition between similar pathogens during co-transmission, the impact of changes in pathogen titer on transmission, and the different approaches used to study co-transmission. Despite recent advancements in techniques, there are still gaps in understanding pathogen transmission. Further laboratory and field studies using different pathosystems are needed to elucidate the role of co-infection and co-transmission in infectious disease ecology and evolution.
Article
Virology
Edson Bertolini, Angela Figas-Segura, Belen Alvarez, Elena G. G. Biosca
Summary: In this study, primers and TaqMan probes were designed, and duplex and multiplex real-time quantitative PCR (qPCR) protocols were developed and optimized for the simultaneous quantification of R. solanacearum and their phages. This method can accurately monitor and quantify these bacteria and phages in water, soil, and plant samples.
Review
Agronomy
Julia Boscariol Rasera, Roberto Fray da Silva, Francisco de Assis Alves Mourao Filho, Alexandre Claudio Botazzo Delbem, Antonio Mauro Saraiva, Paulo Cesar Sentelhas, Patricia Angelica Alves Marques
Summary: This study conducted a bibliometric analysis on the research field of climate change and citrus, revealing that citrus crops are severely affected by climate change and there is a lack of comprehensive research on this topic. The analysis of 178 documents from the Scopus database identified three main research areas: modeling, socio-political issues, and plant physiology. However, there is a need for more studies on predictive models for citrus production under different conditions and climate change scenarios, as well as investigations on combined stresses.
Article
Horticulture
Rodrigo Areze da Silva Santos, Sergio Francisco Schwarz, Magnolia Aparecida Silva da Silva, Edson Bertolini, Camila Cristina Lage de Andrade, Bruna Alana Haupt Pacini
Summary: The Guabiju tree, a member of the Myrtaceae family, has great commercial potential due to its sweet-tasting fresh fruit, which can be used in various food and drink products. This research studied the occurrence and identification of guabiju rust, a disease that affects the plant's stems, leaves, flowers, and fruits. The study also identified the most susceptible and resistant guabiju accessions to the rust pathogen.
REVISTA BRASILEIRA DE FRUTICULTURA
(2022)
Article
Plant Sciences
Daniel Remor Moritz, Neemias da Silva Santos, Francis Zanini, Bruna Alana Pacini, Henrique Belmonte Petry, Edson Bertolini
Summary: The use of vegetable oil in controlling passion fruit woodiness disease shows promising results by reducing virus acquisition and transmission without causing phytotoxic symptoms in plants. Even though Aphis gossypii is highly efficient in transmitting CABMV, the use of vegetable oil significantly decreases the spread of the virus.
TROPICAL PLANT PATHOLOGY
(2022)
Article
Horticulture
Yane Caroline dos Anjos Bezerra, Joao Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Lisia Borges Attilio, Juliana Freitas-Astua, Francisco de Assis Alves Mourao Filho
Summary: New approaches in developing disease-resistant genetically modified organisms have focused on enhancing pathogen control through specific targets for gene expression. Using citrus gene promoters, the study found that tissue-specific promoters resulted in lower gene expression compared to a constitutive promoter, but may lead to more effective expression in certain tissues, potentially increasing disease resistance.
REVISTA BRASILEIRA DE FRUTICULTURA
(2021)
Article
Agronomy
Yuri Caires Ramos, Andre Luiz Fadel, Horst Bremer Neto, Marina Maitto Caputo, Eduardo Sanches Stuchi, Francisco de Assis Alves Mourao Filho
Summary: This study evaluated the horticultural performance of 17 different sweet orange selections compared to the standard cultivar Pera IAC. Several selections showed superior performance in fruit quality and production compared to Pera IAC, making them ideal for the fresh fruit market and juice processing.
EXPERIMENTAL AGRICULTURE
(2021)
Article
Entomology
Jessika Angelotti-Mendonca, Meire M. Bassan, Joao Paulo R. Marques, Pedro T. Yamamoto, Antonio Figueira, Sonia Maria De S. Piedade, Francisco A. A. Mourao Filho
JOURNAL OF ECONOMIC ENTOMOLOGY
(2020)