Article
Microbiology
Marcin Borowicz, Dorota M. Krzyzanowska, Magdalena Narajczyk, Marta Sobolewska, Magdalena Rajewska, Paulina Czaplewska, Katarzyna Wegrzyn, Robert Czajkowski
Summary: Tailocins are nanomolecular machines produced by bacteria with bactericidal activity. In this study, a new tailocin produced by the plant pathogenic bacterium Dickeya dadantii was characterized. The tailocins showed resistance to pH, temperature, and high osmolarity, and had bactericidal activity against certain strains, but were not toxic to other bacteria and nematodes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Robert Czajkowski, Lukasz Rabalski, Przemyslaw Bartnik, Sylwia Jafra
Summary: Dickeya solani is an emerging plant-pathogenic bacterium causing disease symptoms in various agriculturally relevant crop species. Most sequenced genomes of D. solani strains come from potato, but this study presents the first complete high-quality genome of D. solani IPO 2019 isolated from an ornamental plant, providing valuable resources for comparative genomic studies.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Microbiology
Zhenhe Su, Xiaomeng Liu, Qinggang Guo, Lifeng Xuan, Xiuyun Lu, Lihong Dong, Xiaoyun Zhang, Peipei Wang, Weisong Zhao, Yuanhang Qu, Shezeng Li, Ping Ma
Summary: In this study, the pathogenicity of two pectinolytic strains of Pectobacterium spp. (P. brasiliense and P. carotovorum) was investigated. The results showed that P. carotovorum had higher cell wall-degrading enzyme activities and caused more severe disease symptoms in potato tubers compared to P. brasiliense. The pathogenicity of both strains increased with temperature. Furthermore, coinoculation of P. carotovorum with P. brasiliense resulted in more severe pathogenicity, especially when P. carotovorum had a bacterial number advantage at the beginning. These findings provide new insights into the pathogenicity caused by mixed infections of different Pectobacterium spp.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Fan Liu, Ming Hu, Xu Tan, Yang Xue, Chuhao Li, Si Wang, Mingfa Lv, Xiaoyuan Chen, Xiaofan Zhou, Lian-hui Zhang, Jianuan Zhou
Summary: This study constructed a biosensor for monitoring the virulence factor in a specific bacterial strain, Dickeya, and screened out two bacteria with high inhibition efficiency against Dickeya. The results showed that these bacteria can reduce the virulence and PCWDEs production of Dickeya and have the potential for biocontrol of plant diseases.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Entomology
Nisrine Sbai Idrissi, Aicha Ouarzane, Latifa Elouazni, Aziz Hmyene, Said Elantri, Abdessamad Amine
Summary: A study in Morocco isolated 608 bacterial cultures from potato tubers and rhizosphere soils, with 40 showing antagonistic activity against Pectobacterium carotovorum. Among these, 10 isolates exhibited symptom suppression over 90%, with 8 showing complete soft rot suppression in in vivo tests. The selected strains, mainly Bacillus and Pseudomonas species, proved effective in suppressing blackleg and soft rot symptoms, suggesting their potential as biocontrol agents.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2021)
Article
Multidisciplinary Sciences
Przemyslaw Bartnik, Kinga Lewtak, Marta Fiolka, Paulina Czaplewska, Magdalena Narajczyk, Robert Czajkowski
Summary: Resistance to bacteriophage infections in Dickeya solani comes at a cost, compromising its ability to grow and cause disease in potato plants.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Cristina Bez, Alfonso Esposito, Hang Dinh Thuy, Minh Nguyen Hong, Giampiero Vale, Danilo Licastro, Iris Bertani, Silvano Piazza, Vittorio Venturi
Summary: This study focused on the pathobiome analysis of rice foot rot disease caused by Dickeya zeae, revealing the impact of the bacterial pathogen on the resident microbiome and the interactions between the pathogen and the microbial community involved in the disease process. The presence of D. zeae was found to alter the resident bacterial community and lead to the formation of microbial consortia associated with the disease state. Additionally, several bacterial species were identified to be co-present with the pathogen, suggesting their potential involvement in the disease process.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Mariana Galvao Ferrarini, Agnes Vallier, Carole Vincent-Monegat, Elisa Dell'Aglio, Benjamin Gillet, Sandrine Hughes, Ophelie Hurtado, Guy Condemine, Anna Zaidman-Remy, Rita Rebollo, Nicolas Parisot, Abdelaziz Heddi
Summary: This study examined the gene expression of both the host and endosymbiont during the life cycle of the cereal weevil. They found a constant arms race between the host and the endosymbiont, involving various biological functions such as immunity, metabolism, metal control, apoptosis, and bacterial stress response.
Article
Microbiology
Chloe Wasendorf, Stephan Schmitz-Esser, Carter J. Eischeid, Martin J. Leyhe, Erika N. Nelson, Faith M. Rahic-Seggerman, Kasey E. Sullivan, Nick T. Peters
Summary: This study provides the first genomic analysis of E. persicina and compares it to other Erwinia species and P. carotovorum. The research reveals common genomic features and unique characteristics of E. persicina. It offers new insights into the mechanisms of soft rot disease and potential targets for diagnostic tools and control measures.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Gal Sason, Edouard Jurkevitch, Amos Nussinovitch
Summary: This research developed a kappa-carrageenan-based encapsulation system to release predators for preventing soft-rot infections. The encapsulated predators efficiently preyed on soft rot bacteria and had higher survival rates after drying.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ibai Nafarrate, Estibaliz Mateo, Katherine Miranda-Cadena, Amaia Lasagabaster
Summary: This study reported the isolation and characterization of 304 Campylobacter specific bacteriophage isolates from broiler and swine sources. The most promising 59 campylophage candidates were selected based on their lytic activity, and RAPD-PCR technique was found to be effective only for Group II campylophages. Bacteriophage isolates within the same genotype displayed different host ranges, leading to the differentiation of 13 campylophages from Group II and 8 from Group III.
Article
Microbiology
Rebecca D. Curland, Amanda Mainello, Keith L. Perry, Jianjun Hao, Amy O. Charkowski, Carolee T. Bull, Ryan R. McNally, Steven B. Johnson, Noah Rosenzweig, Gary A. Secor, Robert P. Larkin, Beth K. Gugino, Carol A. Ishimaru
Summary: An outbreak of bacterial soft rot and blackleg of potato has been occurring in the northeastern region of the United States since 2014. A phylogenetic study was conducted to identify the pathogens involved in the epidemic, revealing a variety of species clustering with certain type strains. The study also found low genetic diversity in D. dianthicola strains and greater diversity in P. parmentieri strains among those isolated from symptomatic potato samples in 2015-2016.
Article
Agronomy
E. Theron, K. N. Bophela, J. Bisschoff, G. Shin, T. A. Coutinho, J. E. van der Waals
Summary: Pectobacterium brasiliense (Pb) remains the dominant and most pathogenic species on potatoes in South Africa. Pectobacterium carotovorum (Pc), Pectobacterium parmentieri (Pm), and Pectobacterium versatile (Pv) were also identified, with Pv being reported for the first time. Dickeya solani was not detected in any of the samples.
Article
Virology
Maryna Zlatohurska, Tetiana Gorb, Liudmyla Romaniuk, Natalia Shenderovska, Yuliia Faidiuk, Ganna Zhuminska, Yuliia Hubar, Oleksandr Hubar, Andrew M. Kropinski, Alla Kushkina, Fedor Tovkach
Summary: This study characterized the genome of the lytic broad-host-range phage Key infecting Erwinia amylovora, Erwinia horticola, and Pantoea agglomerans strains. The Key phage has a 115,651 bp long double-stranded DNA genome encoding182 proteins and 27 tRNA genes. Most predicted coding sequences (CDSs) encode proteins with unknown functions, and the annotated genes are involved in various biological processes. The similarities to T5-related phages suggest that Key phage represents a novel genus within the Demerecviridae family, tentatively named Keyvirus.
Article
Biotechnology & Applied Microbiology
Zhe Zhou, Xiaozhen Wu, Jiayi Li, Yiyin Zhang, Yaohua Huang, Wenping Zhang, Yu Shi, Junxia Wang, Shaohua Chen
Summary: Quorum sensing (QS) is a microbial communication mechanism that regulates virulence factor production in pathogens. Quorum quenching (QQ) can block QS-mediated microbial infections. This study identified a novel QQ strain, XN-36, which can effectively degrade AHL and reduce the disease severity caused by P. carotovorum in host plants.
BIOLOGICAL CONTROL
(2022)
Article
Agronomy
Agata Motyka-Pomagruk, Sabina Zoledowska, Wojciech Sledz, Ewa Lojkowska
Summary: The study on soft rot Pectobacteriaceae (SRP) in Poland showed that Pectobacterium genus bacteria were abundant and outnumbered Dickeya species. Majority of SRP strains were isolated from potato plants in July 2013 or June-July 2014, mostly from stems rather than tubers. Coinfections of potato samples with multiple SRP species were also observed.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Kaczynska, Ewa Lojkowska, Magdalena Narajczyk, Robert Czajkowski
Summary: This study identified temperature-regulated genes in P. atrosepticum using transposon mutagenesis, uncovering their roles in bacterial metabolism, membrane-related proteins, fitness factors, and hypothetical proteins. Mutants showed alterations in bacterial generation time, biofilm formation, lipopolysaccharide production, and virulence on potato tuber slices, highlighting the impact of environmental temperature on the pathogen's ability to cause disease in potatoes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Izabela Perkowska, Joanna Siwinska, Alexandre Olry, Jeremy Grosjean, Alain Hehn, Frederic Bourgaud, Ewa Lojkowska, Anna Ihnatowicz
Summary: Coumarins are plant phytochemicals induced by stress factors, known for their beneficial properties and importance in pharmaceutical industry. Natural variation in coumarin content in Arabidopsis populations suggests potential for discovering physiological mechanisms involved in their biosynthesis.
Article
Environmental Sciences
Weronika Babinska, Agata Motyka-Pomagruk, Wojciech Sledz, Agnieszka Kowalczyk, Zbigniew Kaczynski, Ewa Lojkowska
Summary: Pectinolytic bacteria from the genus Pectobacterium have been found to cause economic losses in various crops and plants, including potato. A new strain of Pectobacterium aquaticum was isolated and characterized from Pomeranian lakes, showing the ability to degrade plant cell walls and macerate tissues. Monitoring for potential disease-causing agents in natural waterways is essential due to increased irrigation in seed potato fields caused by climate warming.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Agata Motyka-Pomagruk, Anna Dzimitrowicz, Jakub Orlowski, Weronika Babinska, Dominik Terefinko, Michal Rychlowski, Michal Prusinski, Pawel Pohl, Ewa Lojkowska, Piotr Jamroz, Wojciech Sledz
Summary: The study evaluated the effectiveness and mechanism of non-thermal atmospheric pressure plasma (NTAPP) against plant pathogenic bacteria Dickeya and Pectobacterium, noting significant bacterial inactivation rates. Additionally, the NTAPP exposure was found to stimulate germination and early growth of mung bean seeds, highlighting its potential as an eco-friendly and innovative plant protection method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Karolina Ossowska, Agata Motyka-Pomagruk, Natalia Kaczynska, Agnieszka Kowalczyk, Wojciech Sledz, Ewa Lojkowska, Zbigniew Kaczynski
Summary: Pectobacterium parmentieri is a plant pathogenic bacterium causing economic losses in cultivated plants. In this study, it was found that two P. parmentieri strains isolated from different countries have the same OPS structure. Analysis of genomic and phenotypic features revealed that certain strains have higher genome-wide similarity and better ability to macerate tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Przepiora, Donata Figaj, Aleksandra Bogucka, Jakub Fikowicz-Krosko, Robert Czajkowski, Nicole Hugouvieux-Cotte-Pattat, Joanna Skorko-Glonek
Summary: DsbA oxidoreductase plays a crucial role in introducing disulfide bonds in bacteria, and its absence may result in the loss of virulence. In this study, a D. solani dsbA mutant was constructed, and it was found that the lack of DsbA led to the loss of virulence and affected multiple aspects of bacterial physiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Microbiology
Krishna Mohan Poluri, Robert Czajkowski
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Maciag, Dorota M. Krzyzanowska, Lukasz Rabalski, Sylwia Jafra, Robert Czajkowski
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Editorial Material
Plant Sciences
Mohammad Arif, Robert Czajkowski, Toni A. Chapman
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Przemyslaw Bartnik, Kinga Lewtak, Marta Fiolka, Paulina Czaplewska, Magdalena Narajczyk, Robert Czajkowski
Summary: Resistance to bacteriophage infections in Dickeya solani comes at a cost, compromising its ability to grow and cause disease in potato plants.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Microbiology
Zhiyong Li, Robert Czajkowski
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Plant Sciences
Robert Czajkowski, Mohammad Arif, Toni Chapman
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Anissa Yahyaoui, Maroua Oueslati, Agata Motyka-Pomagruk, Natalia Kaczynska, Wojciech Sledz, Belhassen Tarhouni, Mohamed Rabeh Hajlaoui, Ewa Lojkowska, Najla Sadfi-Zouaoui
Summary: Soft rot disease in potato is a global problem, with Pectobacterium and Dickeya bacteria being the main culprits. However, this study reveals the involvement of pectinolytic Bacillus and Paenibacillus in soft rot diseases. Isolates collected from potato samples in Tunisia showed pectinolytic activity and the majority belonged to Bacillus pumilus species. This is the first report of soft rot in potato caused by pectinolytic B. pumilus in Tunisia.
Review
Biochemistry & Molecular Biology
Tomasz Maciag, Edmund Koziel, Piotr Rusin, Katarzyna Otulak-Koziel, Sylwia Jafra, Robert Czajkowski
Summary: Biological plant protection is a promising alternative to chemical methods for safeguarding plants against diseases, using biological control agents (BCAs) to suppress plant pathogens. While microbial BCAs have the potential to effectively manage crop diseases, there is a need for further research to improve their efficacy. Microbial consortia, consisting of multiple microorganisms with different mechanisms of action, offer promise in enhancing the overall antipathogen effect. This article aims to present an analysis of the current status of microbial consortia-based biocontrol and explore future research directions for biological plant protection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)