Article
Biochemistry & Molecular Biology
Peter Braun, Martin Duy-Thanh Nguyen, Mathias C. Walter, Gregor Grass
Summary: A real-time PCR assay targeting a specific single nucleotide polymorphism in Bacillus anthracis genomes was validated, showing high specificity, linearity, and sensitivity. Adapting the assay for reverse transcription PCR on 16S rRNA transcripts significantly increased sensitivity compared to DNA-targeting assays, offering great potential for molecular diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Physiology
Jinrong Tang, Gemei Liang, Shaoqi Dong, Shuang Shan, Man Zhao, Xianru Guo
Summary: By evaluating the stability of reference genes in Athetis dissimilis under different conditions, suitable reference genes for different experimental conditions can be determined. Different reference genes are needed under different experimental conditions, providing a reliable basis for studying gene expression in A. dissimilis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medical Laboratory Technology
Luca Bernasconi, Peter Neyer, Michael Oberle, Bettina Schmid, Eileen Martin, Hans Fankhauser, Sebastian Haubitz, Angelika Hammerer-Lercher
Summary: This study assessed the diagnostic accuracy of a new fully automated SARS-CoV-2 antigen assay and explored the efficiency of a diagnostic algorithm combining antigen and molecular assays. The results showed that the assay had a sensitivity of 79.1% and specificity of 97.8%. By combining the antigen assay with RT-PCR, the number of tests could be reduced by 36.1% during the pandemic phase, leading to cost and time savings.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Veterinary Sciences
Siti Tasnim Makhtar, Sheau Wei Tan, Nur Amalina Nasruddin, Nor Azlina Abdul Aziz, Abdul Rahman Omar, Farina Mustaffa-Kamal
Summary: A specific TaqMan-based real-time RT-PCR assay targeting the N gene of FeMV was developed in this study, showing higher detection rate and sensitivity in early detection of FeMV infection.
BMC VETERINARY RESEARCH
(2021)
Article
Infectious Diseases
Georgi Tomov, Nikola Stamenov, Deyan Neychev, Kiril Atliev
Summary: This study aimed to examine the possible association between tongue piercing and Candida spp. infection in healthy individuals. The results suggest that the main risk factors for tongue colonization with Candida spp. are gender, smoking, and improper hygiene, while tongue piercing is not a risk factor for colonization of Candida spp.
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Medicine, Research & Experimental
Xi Wang, Yixiao Feng, Shixian Zhou, Xiaorong Yang, Yan Liu, Qi Peng, Xuehua Kong, Lan Zhou, Zongyue Zeng
Summary: The study demonstrates that size selection magnetic beads can efficiently remove long RNA transcripts and concentrate small RNAs for reverse transcription, while touchdown qPCR improves microRNA detection efficiency, particularly for low-abundance microRNAs. The new protocol LTMT-qPCR, integrating these techniques, shows higher precision and detection efficiency compared to traditional methods, making it more suitable for microRNA screening and quantification.
LABORATORY INVESTIGATION
(2021)
Article
Oncology
Aileen I. Fernandez, Niki Gavrielatou, Leena McCann, Saba Shafi, Myrto K. Moutafi, Sandra Martinez-Morilla, Ioannis A. Vathiotis, Thazin Nwe Aung, Vesal Yaghoobi, Yalai Bai, Yvonne G. Chan, Jodi Weidler, Roy Herbst, Michael Bates, David L. Rimm
Summary: This study investigates the use of PD-L1 mRNA level as a predictive biomarker for nonresponse to immune checkpoint inhibitors (ICIs) in lung cancer patients. The results show that high levels of PD-L1 and PD-L2 mRNA are associated with improved long-term benefit and overall survival, while low PD-L1 mRNA level has a high negative predictive value for absence of long-term benefit.
JOURNAL OF THORACIC ONCOLOGY
(2022)
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Plant Sciences
Xiao-Ying Li, Lin Gao, Wei-Hong Zhang, Jing-Kun Liu, Yan-Jun Zhang, Hai-Yan Wang, Da-Qun Liu
JOURNAL OF PLANT INTERACTIONS
(2015)
Article
Biochemistry & Molecular Biology
Lin Gao, Shen Wang, Yanjun Zhang, Xiaoying Li, Haiyan Wang, Daqun Liu
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2016)
Article
Plant Sciences
Parham Haddadi, Lisong Ma, Haiyan Wang, M. Hossein Borhan
MOLECULAR PLANT PATHOLOGY
(2016)
Article
Biochemical Research Methods
Xiaoying Li, Yanjun Zhang, Weihong Zhang, Jiarui Zhang, Haiyan Wang, Daqun Liu
PLANT MOLECULAR BIOLOGY REPORTER
(2016)
Article
Plant Sciences
Yanjun Zhang, Haixia Yan, Xuejun Wei, Jiarui Zhang, Haiyan Wang, Daqun Liu
JOURNAL OF PLANT INTERACTIONS
(2017)
Article
Plant Sciences
Jiarui Zhang, Fei Wang, Fang Liang, Yanjun Zhang, Lisong Ma, Haiyan Wang, Daqun Liu
Article
Agriculture, Multidisciplinary
Zhang Nan, Wang Shen, Wang Hai-yan, Liu Da-qun
AGRICULTURAL SCIENCES IN CHINA
(2011)
Article
Genetics & Heredity
Fei Wang, Shitao Yuan, Wenyue Wu, Yiqing Yang, Zhongchi Cui, Haiyan Wang, Daqun Liu
Editorial Material
Plant Sciences
Xinkang Sun, Shitao Yuan, Songsong Shen, Huaimin Geng, Junying Liu, Zhongchi Cui, Fei Wang, Guoshu Wei, Daqun Liu, Haiyan Wang