Article
Entomology
Kyle M. Bekelja, Kathleen M. Miller, Thomas P. Kuhar, Christian H. Krupke, Sally Taylor
Summary: Nearly all maize seed sold in the US contains neonicotinoid seed treatment to protect against early-season insect pests. Bacillus thuringiensis (Bt) proteins are used as alternatives to insecticides. Non-Bt refuges are used to preserve susceptible alleles in the population. This study found that neonicotinoid seed treatments had inconsistent effects on refuge beetles and did not provide significant agronomic advantages over Bt seed alone.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Agronomy
Dawson D. Kerns, Fei Yang, David L. Kerns, Scott D. Stewart
Summary: The study aimed to determine if plant tissue overlays could detect resistance by using Bt proteins and Bt cotton plant tissue in diet-overlay bioassays. Results showed that overlays using lyophilized tissue from white flowers were most ideal, while tissue from non-Bt bolls and leaves affected larval health and behavior, affecting assay results.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Haley Kennedy, David L. Kerns, Graham P. Head, Fei Yang
Summary: Our findings show that Vip3Aa-resistant H. zea does not exhibit positive cross-resistance to Cry1 or Cry2 proteins. Furthermore, most pyramided Bt crops have a complete or nearly complete redundant killing effect on Vip3Aa-resistant H. zea. These results suggest that the pyramiding strategy is often effective for managing Vip3Aa resistance in regions of the United States where H. zea has not developed resistance to Cry1 and Cry2 toxins. (c) 2023 Society of Chemical Industry.
PEST MANAGEMENT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Fernando Alvarez-Alfageme, Yann Devos, Ana M. Camargo, Salvatore Arpaia, Antoine Messean
Summary: Since 1998, genetically engineered Bt maize varieties have been grown in Spain to resist corn borers without reported resistance or unexpected field damage. However, the sensitivity of the resistance monitoring strategy is debated, and some areas exceed recommended resistance allele frequencies. To ensure the durability of Bt maize in Spain, continued implementation of appropriate resistance management strategies is crucial.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Entomology
Kyle J. Paddock, Kyle Dellamano, Bruce E. Hibbard, Kent S. Shelby
Summary: Insect resistance to Bacillus thuringiensis (Bt) toxins is a major concern in agriculture, and resistance mechanisms include loss of toxin binding sites, changes in gut microbiota, and midgut tissue regeneration. This study provides histopathological evidence for the first time showing the morphological response of Bt-resistant and Bt-susceptible western corn rootworm larvae to Bt intoxication, shedding light on the multifaceted nature of Bt resistance in these insects.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Debora G. Montezano, Thomas E. Hunt, Priscila M. Colombo da Luz, Kelsey Karnik, Stephen D. Kachman, Ana M. Velez, Julie A. Peterson
Summary: This study investigated the behavior of the western bean cutworm larvae when exposed to Bt plants. The results showed that the larvae were more likely to abandon plants that expressed Bt proteins. This finding is important for the use of integrated versus structured refuges.
Article
Agronomy
Arun Babu, Dominic D. Reisig, Maiquel P. Pes, Christopher M. Ranger, Narong Chamkasem, Michael E. Reding
Summary: Results from field and laboratory experiments showed that there were no interactions between Bt and chlorantraniliprole in influencing larval survival or growth parameters. Chlorantraniliprole had long-residual effects on H. zea larvae feeding on cotton leaves, but its utility as a Bt-resistance management tool remains unclear.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agronomy
Xihua Liu, Yile Zhang, Xi Du, Xingyu Luo, Weilong Tan, Xiong Guan, Lingling Zhang
Summary: This study showed that deletion of the yhfS gene in Bacillus thuringiensis (Bt) led to metabolic disorders and reduced antioxidant ability, resulting in decreased resistance to UV radiation.
PEST MANAGEMENT SCIENCE
(2023)
Article
Environmental Sciences
Michael Meissle, Stefanie Kloos, Joerg Romeis
Summary: Insects such as lady beetles can ingest Cry proteins from genetically engineered crops without significant accumulation in their bodies, and the proteins are quickly degraded and excreted. The impact of Bt maize on lady beetle larvae and adults is minimal in terms of Cry protein exposure.
ENVIRONMENTAL POLLUTION
(2021)
Article
Agronomy
Fei Yang, Jose C. Santiago Gonzalez, Gregory A. Sword, David L. Kerns
Summary: The study revealed that resistance to Vip3Aa in H. zea is monogenic, autosomal, and recessive, with no maternal effects or sex linkage observed. Resistance is largely determined by a single gene, providing valuable insights for understanding the resistance mechanism and developing effective resistance management strategies.
PEST MANAGEMENT SCIENCE
(2021)
Article
Chemistry, Analytical
Liyuan Zhang, Runzhong Yu, Yingbo Yu
Summary: Gas chromatography-mass spectrometry and non-targeted metabolomics techniques were used to analyze the metabolites and metabolic pathways of non-transgenic and Bacillus thuringiensis transgenic maize. The results showed that the transgenic maize had more metabolites involved in metabolic pathways and higher activity levels compared to non-transgenic maize.
MICROCHEMICAL JOURNAL
(2021)
Article
Entomology
Bruce E. Tabashnik, Jeffrey A. Fabrick, Yves Carriere
Summary: Genetically engineered crops producing insecticidal proteins have improved pest management, but practical resistance has reduced their efficacy. Resistance has been documented in 11 pest species affecting seven countries, while 16 pest species remain susceptible. Early warnings of resistance have been observed, but further research is needed.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wendy Kain, Rey O. Cotto-Rivera, Ping Wang
Summary: This study focuses on the selection and isolation of Trichoplusia ni strains resistant to Bt toxins Cry1Ac and Cry1F, as well as the genetic characterization of Cry1F resistance trait in the strains. The findings provide important insights into the development of resistance management technologies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Agronomy
Yaling Lin, Qing Gao, Yueqin Wang, Zhenying Wang, Kanglai He, Suqin Shang, Tiantao Zhang
Summary: This study successfully developed Cry1le and Cry1F-resistant strains of Asian corn borer through laboratory selection. Transcriptome sequencing revealed significant down-regulation of multiple genes in the resistant strains. Validation of these differentially expressed genes provides candidate genes for further research on Bt resistance mechanism.
Article
Chemistry, Multidisciplinary
Yujun Long, Haiyan Zhang, Guohui Liao, Minggui Chen, Xiangyan Chen, Lixin Qin, Caijun Chen, Zhuo Chen, Xiaomao Wu, Feng Zhu
Summary: Broadcasting emamectin benzoate (EB) granules into maize leaf whorls effectively controls fall armyworm (FAW). However, the distribution of EB in maize plants is unclear. In this study, EB granules were prepared and characterized using infrared spectrometry. The behavior of the granules in water and the release of EB were studied, and a method for determining EB in different parts of maize plants was established.