Article
Biochemistry & Molecular Biology
Baosheng Liao, Xiaofeng Shen, Li Xiang, Shuai Guo, Shiyu Chen, Ying Meng, Yu Liang, Dandan Ding, Junqi Bai, Dong Zhang, Tomasz Czechowski, Yi Li, Hui Yao, Tingyu Ma, Caroline Howard, Chao Sun, Haitao Liu, Jiushi Liu, Jin Pei, Jihai Gao, Jigang Wang, Xiaohui Qiu, Zhihai Huang, Hongyi Li, Ling Yuan, Jianhe Wei, Ian Graham, Jiang Xu, Boli Zhang, Shilin Chen
Summary: This study investigates the biosynthesis and regulation of artemisinin through chromosome-level genome mapping. The research discovers a correlation between artemisinin content and the copy number of amorpha-4,11-diene synthase genes, providing new insights into the production of this anti-malarial drug.
Review
Biochemistry & Molecular Biology
Jameel M. Al-Khayri, Wudali N. Sudheer, Vasantha V. Lakshmaiah, Epsita Mukherjee, Aatika Nizam, Muthu Thiruvengadam, Praveen Nagella, Fatima M. Alessa, Muneera Q. Al-Mssallem, Adel A. Rezk, Wael F. Shehata, Mahesh Attimarad
Summary: This review primarily focuses on the biosynthesis of artemisinin and the methods to increase its production using tissue culture techniques.
Article
Plant Sciences
Tian-Tian Chen, Xing-Hao Yao, Hang Liu, Yong-Peng Li, Wei Qin, Xin Yan, Xiu-Yun Wang, Bo-Wen Peng, Yao-Jie Zhang, Jin Shao, Xin-Yi Hu, Qing Miao, Xue-Qing Fu, Yu-Liang Wang, Ling Li, Ke-Xuan Tang
Summary: A MADS transcription factor, AaSEP4, has been identified as a positive regulator of artemisinin biosynthesis in Artemisia annua. AaSEP4 activates the expression of GLANDULAR TRICHOME-SPECIFIC WRKY1 and directly binds to its promoter region. Overexpression of AaSEP4 significantly increases artemisinin content in A. annua, making it a potential target for enhancing artemisinin production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xingxing Wang, Wenjing Sun, Shiyuan Fang, Boran Dong, JinXing Li, Zongyou Lv, Wankui Li, Wansheng Chen
Summary: Artemisinin, extracted from Artemisia annua L., is an important drug for malaria treatment and has potential applications in other diseases. The regulatory mechanism of artemisinin production is still not well understood. In this study, WRKY6 was identified as a key transcription factor that binds to the promoter of the artemisinin biosynthesis gene DBR2, thereby regulating artemisinin synthesis and content during the growth cycle of A. annua.
Article
Agricultural Engineering
Jose Abramo Marchese, Jorge F. S. Ferreira, Rita Maria Moraes, Franck E. Dayan, Michelle F. F. Rodrigues, Jorge Jamhour, Lucas Vinicius Dallacorte
Summary: In this study, a phenological scale was established for Artemisia annua L. to facilitate successful cross-pollination and breeding. The scale divided the plant's growth cycle into the vegetative phase and reproductive phase, with distinct stages identified through daily observations and photographs. The experiments showed that genotypes from different geographic origins demonstrate better synchronization under both short days and low temperatures, and the concentration and yield of artemisinin vary depending on the photoperiod and growth phase.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Biotechnology & Applied Microbiology
Jin Quan Huang, Xin Fang
Summary: ADS is the key enzyme in the artemisinin biosynthetic pathway, playing a crucial role in the production of artemisinin. The microbial production of amorpha-4,11-diene signifies a breakthrough in metabolic engineering and synthetic biology. Various new techniques have been utilized in ADS engineering to explore its potential applications in chemoenzymatic production of artemisinin.
Article
Plant Sciences
Boran Dong, Xingxing Wang, Rui Jiang, Shiyuan Fang, Jinxing Li, Qing Li, Zong You Lv, Wan Sheng Chen
Summary: In this study, AaCycTL was found to regulate trichome development and affect the content of artemisinin in Artemisia annua. Overexpression of AaCycTL in Arabidopsis and A. annua resulted in changes in wax coverage on the plant surface and decreased trichome density. The interaction between AaCycTL and AaTAR1 was identified, indicating a potential role of AaCycTL in regulating trichome density and artemisinin biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Umit Babacan, Mehmet Fatih Cengiz, Mariem Bouali, Timur Tongur, Songul Sever Mutlu, Enes Gulmez
Summary: Artemisinin, the main active compound in Artemisia annua L., has been found to have antimalarial and antiviral properties. This study successfully optimized a HPLC method for the determination of artemisinin content in A. annua samples and also optimized the extraction conditions for the compound.
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS
(2022)
Article
Plant Sciences
Shuwei Chang, Qi Li, Baokang Huang, Wansheng Chen, Hexin Tan
Summary: This study systematically identified and characterized 226 putative bHLH transcription factors in A. annua. These transcription factors play important roles in regulating key biological processes such as artemisinin biosynthesis and trichome development.
Article
Horticulture
Federica Blando, Francesca Rizzello, Miriana Durante, Angelo De Paolis, Sofia Caretto, Giovanni Mita
Summary: This study established an in vitro regeneration process of Artemisia annua L. and investigated the potential of tissue culture to induce new variability in artemisinin metabolism. The results showed that adventitious shoot induction could provide a useful strategy to impact artemisinin metabolism through in vitro manipulation.
Review
Agricultural Engineering
Ranjana Soni, Gauri Shankar, Pradipto Mukhopadhyay, Vikrant Gupta
Summary: Malaria is a major global health concern and previous measures to control and cure it are being hampered by drug resistance. Artemisinin derived from sweet wormwood is highly effective against the malaria-causing parasite and is a major component of current treatments. However, there is a limited supply of artemisinin, and novel strategies and advanced scientific methods are needed to meet global demand.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Zhaoyu Liu, Yupeng Du, Zhihao Sun, Bohan Cheng, Zenghao Bi, Zhicheng Yao, Yuting Liang, Huiling Zhang, Run Yao, Shen Kang, Yuhua Shi, Huihua Wan, Dou Qin, Li Xiang, Liang Leng, Shilin Chen
Summary: Gene annotation is crucial for genome-based research, but algorithm-based annotation may not accurately reveal genomic information for species with complex genomes. In this study, we manually corrected the genome annotation of Artemisia annua using transcriptome data and found that gene annotation errors can affect gene structure, function, and expression levels.
Article
Plant Sciences
Xingwen Wang, Lan Wu, Li Xiang, Ranran Gao, Qinggang Yin, Mengyue Wang, Zhaoyu Liu, Liang Leng, Yanyan Su, Huihua Wan, Tingyu Ma, Shilin Chen, Yuhua Shi
Summary: This study compared the differences between the LAP and HAP chemotypes of Artemisia annua, the plant source of artemisinin, through comprehensive analysis of genome resequencing, metabolome, and transcriptome data. The results showed that the HAP chemotype had a higher accumulation of sesquiterpenes compared to the LAP chemotype. A gene called DBR2-Like (DBR2L) was found to be potentially involved in artemisinin biosynthesis. The analysis of DBR2L promoters revealed complicated variations among different varieties of A. annua, which may affect the expression level and yield of artemisinin.
HORTICULTURE RESEARCH
(2023)
Article
Engineering, Environmental
Kaiser Iqbal Wani, M. Naeem, M. Masroor A. Khan, Tariq Aftab
Summary: This study investigates the role of strigolactones (SLs) in alleviating cadmium (Cd) toxicity in Artemisia annua plants. The results show that SLs improve the photosynthetic performance and Cd tolerance by modulating the antioxidant enzyme system, protecting chloroplasts and pigments, and enhancing glandular trichome (GT) attributes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Agriculture, Multidisciplinary
Jin-Quan Huang, Dong-Mei Li, Xiu Tian, Jia-Ling Lin, Lei Yang, Jing-Jing Xu, Xin Fang
Summary: ADS is involved in the biosynthesis of artemisinin through biobased fermentation. Chiral analysis showed the production of amorpha-4-en-11-ol by ADS as a pair of epimers. Labeling experiments indicated the co-occurrence of initial 1,6 and 1,10 cyclization of farnesyl diphosphate by ADS mutants.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
L. Aranzazu Diaz-Ramos, Andrew O'Hara, Selvaraju Kanagarajan, Daniel Farkas, Ake Strid, Gareth Jenkins
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2018)
Article
Multidisciplinary Sciences
Magnus L. R. Carlsson, Selvaraju Kanagarajan, Leif Bulow, Li-Hua Zhu
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Saraladevi Muthusamy, Ramesh R. Vetukuri, Anneli Lundgren, Suresh Ganji, Li-Hua Zhu, Peter E. Brodelius, Selvaraju Kanagarajan
Article
Plant Sciences
Magnus L. R. Carlsson, Amanda Kristiansson, Jesper Bergwik, Selvaraju Kanagarajan, Leif Bulow, Bo Akerstrom, Li-Hua Zhu
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Selvaraju Kanagarajan, Magnus L. R. Carlsson, Sandeep Chakane, Karin Kettisen, Emanuel Smeds, Ranjeet Kumar, Niklas Ortenlof, Magnus Gram, Bo Akerstrom, Leif Bulow, Li-Hua Zhu
Summary: This study successfully produced recombinantly fused endotoxin-free HbF in Nicotiana benthamiana through Agrobacterium tumefaciens-mediated transient expression, demonstrating its high potential for use as blood substitutes. The recombinant protein showed structural and functional properties similar to native HbF, confirmed by various analyses including SDS-PAGE, Western blot, and mass spectrometry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Xueyuan Li, Sjur Sandgrind, Oliver Moss, Rui Guan, Emelie Ivarson, Eu Sheng Wang, Selvaraju Kanagarajan, Li-Hua Zhu
Summary: This study established a rapid and efficient protocol for the isolation, regeneration, and transfection of protoplasts of rapeseed, investigating different basal media, types and combinations of plant growth regulators, and protoplast culture duration. Optimized combinations in media led to up to 45% shoot regeneration, with a critical importance of culture duration on different media. The transfection protocol was also successfully optimized for high mutation frequency in rapeseed gene editing.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Rajaprakasam Sudhagar, Shanmugavel Priyanka, Vanniarajan Chockalingam, Vaithiyanathan Sendhilvel, Jegadeesan Souframanien, Kalimuthu Raja, Selvaraju Kanagarajan
Summary: This study explored the effects of powdery mildew on horse gram growth and yield through screening mutants and conducting metabolomic analysis. The research found differences in metabolite expression among different mutants and the accumulation of certain compounds related to disease resistance.
Article
Nanoscience & Nanotechnology
Kamaljit Kaur, Bandana Kumari Sahu, Kanchan Swami, Mahima Chandel, Anshika Gupta, Li-Hua Zhu, Jeffrey P. Youngblood, Selvaraju Kanagarajan, Vijayakumar Shanmugam
Summary: A transparent upconversion nanoparticles strip has been developed for real-time monitoring of lycopene concentration. The strip shows a low detection limit and increased hydrophobicity during fabrication, making it a potential extraction-free substitute for chromatography. Additionally, the strip exhibits a high luminescence recovery rate.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Sudhagar Rajaprakasam, Priyanka Shanmugavel, Vanniarajan Chockalingam, Souframanien Jegadeesan, Tnpalayam Krishnaswamy Sukirtha Latha, Saravanan Naaganoor Ananthan, Raveendran Muthurajan, Selvaraju Kanagarajan
Summary: This study investigated the resistance of 252 horse gram germplasm accessions to horse gram yellow mosaic virus. The results showed that the percentage of highly resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible accessions were 0.34%, 13.89%, 38.89%, 46.43%, and 0.34% respectively. Further analysis revealed an enhanced accumulation of sugars, alkanes, and carboxylic acids in the highly resistant genotypes.
Article
Genetics & Heredity
Misteru Tesfaye, Tileye Feyissa, Teklehaimanot Hailesilassie, Selvaraju Kanagarajan, Li-Hua Zhu
Summary: This study investigated the genetic diversity and population structure of Ethiopian mustard (Brassica carinata) genotypes of different origins using high-throughput single nucleotide polymorphism (SNP) markers. The results revealed two distinct subpopulations, with the majority of accessions clustered in one subpopulation mainly obtained from specific regions. The analysis showed high genetic variation within populations and low genetic differentiation between populations, indicating the presence of gene flow and weak geographical structure. The study provides important information for future breeding and conservation efforts of B. carinata.
Review
Agronomy
Karthikeyan Ramasamy, Kalarani M. Karuppasami, Senthil Alagarswamy, Kavitha P. Shanmugam, Sivakumar Rathinavelu, Geethalakshmi Vellingiri, Umapathi Muniyappan, Thirukumaran Kanthan, Anitha Kuppusamy, Megala Rajendran, Arunkumar Kathirvel, Selvaraju Kanagarajan
Summary: Melatonin, a naturally occurring compound, plays a crucial role in plant growth and development, as well as in the regulation of physiological functions in response to abiotic stresses. This compound acts as an antioxidant, protecting plants from oxidative damage caused by reactive oxygen species. By enhancing the plant antioxidant defense system, melatonin improves plant growth and development under various abiotic stresses such as drought, salinity, high temperature, flooding, and heavy metals. The understanding of melatonin's role in plant biology is important for developing strategies to enhance the resilience of crops in the face of climate change.
Article
Plant Sciences
Anitha Kuppusamy, Senthil Alagarswamy, Kalarani M. Karuppusami, Djanaguiraman Maduraimuthu, Senthil Natesan, Kuttimani Ramalingam, Umapathi Muniyappan, Marimuthu Subramanian, Selvaraju Kanagarajan
Summary: Mung bean is sensitive to abiotic stresses, and its yield potential is affected by drought and high-temperature stress. Melatonin plays a vital role in plant stress defense mechanisms. This study evaluated the efficiency of melatonin under individual and combined drought and high-temperature stress in mung bean, and found that foliar application of melatonin improves physiological and yield-related traits by enhancing antioxidant enzyme activity and metabolite concentration.
Article
Chemistry, Multidisciplinary
Raja Kalimuthu, Kumuthan Meenachi Sellan, Dhivya Antony, Sudhagar Rajaprakasam, Vanniarajan Chokkalingam, Prabu Chidambaram, Selvaraju Kanagarajan
Summary: Bare and stabilized zinc oxide nanoparticles were prepared using a microwave-assisted method, and used as a priming agent to improve the quality of Vigna radiata under normal and moisture stress conditions. The priming effect was found to be more effective when the zinc oxide nanoparticles were capped with cysteine, leading to improvements in germination, seedling vigor, and enzyme activity.
Review
Biochemistry & Molecular Biology
Sudha Manickam, Veera Ranjani Rajagopalan, Rohit Kambale, Raghu Rajasekaran, Selvaraju Kanagarajan, Raveendran Muthurajan
Summary: Plant metabolomics is a field that involves comprehensive analyses of small molecules in plant tissues and cells, providing insights into the functional roles of metabolites and their associations with specific traits. Integrating metabolomics data with other omics data enables researchers to understand the complex interactions within organisms.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Sandeep Sharma, Mohd. Muddassir, Saraladevi Muthusamy, Pardeep Kumar Vaishnav, Manish Singh, Deepak Sharma, Selvaraju Kanagarajan, Vijayakumar Shanmugam
SCIENTIFIC REPORTS
(2020)