Journal
PLANT MOLECULAR BIOLOGY
Volume 76, Issue 1-2, Pages 85-96Publisher
SPRINGER
DOI: 10.1007/s11103-011-9770-4
Keywords
ESCRT; Arabidopsis; Protein-protein network; Endosomal sorting; MVB; Protein trafficking
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Funding
- DFG [SFB 635]
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In yeast, endosomal sorting of monoubiquitylated transmembrane proteins is performed by a subset of the 19 class E vacuolar protein sorting proteins. The core machinery consists of 11 proteins that are organised in three complexes termed ESCRT I-III (endosomal sorting complex required for transport I-III) and is conserved in eukaryotic cells. While the pathway is well understood in yeast and animals, the plant ESCRT system is largely unexplored. At least one sequence homolog for each ESCRT component can be found in the Arabidopsis genome. Generally, sequence conservation between yeast/animals and the Arabidopsis proteins is low. To understand details about participating proteins and complex organization we have performed a systematic pairwise yeast two hybrid analysis of all Arabidopsis proteins showing homology to the ESCRT core machinery. Positive interactions were validated using bimolecular fluorescence complementation. In our experiments, most putative ESCRT components exhibited interactions with other ESCRT components that could be shown to occur on endosomes suggesting that despite their low homology to their yeast and animal counterparts they represent functional components of the plant ESCRT pathway.
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