Article
Biotechnology & Applied Microbiology
Linghao Hu, Nianchao Wang, Joshua Bryant, Lin Liu, Linglin Xie, A. Phillip West, Alex Walsh
Summary: Autofluorescence lifetime imaging combined with machine learning models can detect metabolic perturbations between glycolysis and oxidative metabolism of living samples at a cellular level, providing a label-free technology to study cellular metabolism and metabolic heterogeneity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biophysics
Leonel Malacrida, Suman Ranjit, David M. Jameson, Enrico Gratton
Summary: The phasor approach in fluorescence lifetime imaging has become a common method to analyze complex fluorescence signals in biological samples, providing insights into fundamental biological states and molecular interactions. It offers new avenues for disease characterization and diagnostics.
ANNUAL REVIEW OF BIOPHYSICS, VOL 50, 2021
(2021)
Article
Plant Sciences
Nina Gloeckner, Sven zur Oven-Krockhaus, Leander Rohr, Frank Wackenhut, Moritz Burmeister, Friederike Wanke, Eleonore Holzwart, Alfred J. Meixner, Sebastian Wolf, Klaus Harter
Summary: Protein-protein interaction studies are crucial for understanding cellular signaling. This study used three-fluorophore FRET and FRET-fluorescence lifetime imaging microscopy techniques to demonstrate the formation of a ternary complex involving Receptor-Like Protein 44, Brassinosteroid Insensitive 1, and Brassinosteroid Insensitive 1 Associated Kinase 1 in living plant cells. The researchers also found that this complex is localized in a distinct plasma membrane nanodomain.
Article
Chemistry, Multidisciplinary
Pia A. Lopez, Vy H. B. Pham, Suzanne A. Blum
Summary: A new autofluorescence technique was developed to monitor polymerization progress in real time/in line without typical fluorogenic groups. By harnessing the autofluorescence of dicyclopentadiene and polydicyclopentadiene, the reaction progress of ruthenium-catalyzed ring-opening metathesis polymerization could be quantitatively evaluated. This method has the potential to be extended to other polymerization reactions previously overlooked due to the lack of obvious fluorescence handles.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Adrian Monteleone, Weronika Schary, Folker Wenzel, Heinz Langhals, Daniel R. Dietrich
Summary: The study introduced a new method FLIM and phasor analysis for fast, label-free, and sensitive identification and differentiation of plastics, which represents a significant advancement in addressing the issue of plastic pollution.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Biotechnology & Applied Microbiology
Tiffany M. Heaster, Alexa R. Heaton, Paul M. Sondel, Melissa C. Skala
Summary: Metabolic autofluorescence imaging was used to assess cell-level macrophage heterogeneity in response to normal and cancerous tissue microenvironments in vivo. Tumor-associated macrophages exhibited higher oxidation levels compared to dermal macrophages, with changes in NAD(P)H and FAD protein-binding activities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
William N. Zagotta, Brandon S. Sim, Anthony K. Nhim, Marium M. Raza, Eric G. B. Evans, Yarra Venkatesh, Chloe M. Jones, Ryan A. Mehl, E. James Petersson, Sharona E. Gordon
Summary: With the recent explosion in high-resolution protein structures, there is a new frontier in biology to elucidate the mechanisms of conformational rearrangements in proteins to meet the changing needs of cells. By rigorously measuring protein energetics and dynamics through new methods like tmFRET, differences in conformational states and accurate quantification of distributions can be revealed. This new approach using Acd for time-resolved tmFRET sets the stage for measuring the energetics of conformational rearrangements in soluble and membrane proteins in near-native conditions.
Article
Biochemistry & Molecular Biology
Alba Alfonso-Garcia, Stephanie A. Cevallos, Jee-Yon Lee, Cai Li, Julien Bec, Andreas J. Baumler, Laura Marcu
Summary: This study tested the feasibility of using fluorescence lifetime imaging (FLIm) to non-destructively detect inflammation in thick tissue. The results showed that FLIm was sensitive to colon anatomy and colonocyte layer, and could detect changes in epithelial bioenergetics and physical structure related to inflammatory disease treatment.
Article
Biochemistry & Molecular Biology
Bengt Svensson, Florentin R. Nitu, Robyn T. Rebbeck, Lindsey M. Mcgurran, Tetsuro Oda, David D. Thomas, Donald M. Bers, Razvan L. Cornea
Summary: Current studies have shown that Ca2+ leakage from cardiomyocyte sarcoplasmic reticulum through hyperactive resting cardiac ryanodine receptor channels (RyR2) has a pro-arrhythmic effect. An exogenous peptide (DPc10) binding promotes leaky RyR2 in cardiomyocytes and reports on the endogenous state. Conversely, binding of calmodulin (CaM) inhibits RyR2 leak and low CaM affinity is indicative of leaky RyR2. These findings have led to the development of a FRET biosensor for drug discovery targeting RyR2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Amani A. Gillette, Christopher P. Babiarz, Ava R. VanDommelen, Cheri A. Pasch, Linda Clipson, Kristina A. Matkowskyj, Dustin A. Deming, Melissa C. Skala
Summary: Gastroenteropancreatic neuroendocrine tumors (GEP-NET) make up around 60% of all neuroendocrine tumors, with low/intermediate grade human GEP-NETs having slow growth rates that traditional laboratory culture methods struggle to capture. The study explores the use of a label-free, non-destructive optical metabolic imaging (OMI) method to measure drug response in live GEP-NET patient-derived cancer organoids (PDCOs), showing promising results in testing drug combinations for GEP-NET treatment.
Article
Biochemistry & Molecular Biology
Nicoleta Sandu, Claudia G. Chilom, Melinda David, Monica Florescu
Summary: This study investigated the interaction between the hydrophilic drug levothyroxine (LT4) and bovine serum albumin (BSA). Various techniques including UV-vis measurements, Fourier-transform infrared spectroscopy (FT-IR), fluorescence, fluorescence resonance energy transfer (FRET), Surface Plasmon Resonance (SPR), and molecular docking analysis were used. The results showed that LT4 binds to BSA through ground-state complex formation, driven mainly by hydrogen bonds and van der Waals forces. The immobilization of BSA was found to be crucial for the binding of LT4. These findings have important implications for understanding the pharmacological significance of LT4-protein binding.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Oncology
Maria Lukina, Konstantin Yashin, Elena E. Kiseleva, Anna Alekseeva, Varvara Dudenkova, Elena V. Zagaynova, Evgenia Bederina, Igor Medyanic, Wolfgang Becker, Deependra Mishra, Mikhail Berezin, Vladislav I. Shcheslavskiy, Marina Shirmanova
Summary: The development of a rapid and sensitive assay for intraoperative diagnostics of glioma, focusing on fluorescence lifetime imaging (FLIM) to identify imaging markers for differentiation between tumors and healthy tissues, shows a high potential for diagnostics of glioma and evaluation of surgical margins.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Rebecca Schmitz, Kelsey Tweed, Christine Walsh, Alex J. Walsh, Melissa C. Skala
Summary: The study investigated the effect of extracellular pH on the fluorescence lifetimes of FAD, FMN, and NAD(P)H. Results showed that FAD and NAD(P)H mean lifetimes increased and decreased, respectively, with increasing pH, with differences observed between the two cell lines.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Physics, Multidisciplinary
Jesus Sambrano, Felicia Rodriguez, John Martin, Jessica P. Houston
Summary: Conventional flow cytometry is a valuable quantitative tool that provides users with multiparametric data and high-throughput cell information. Time-resolved flow cytometry plays an important role in fluorescence decay kinetics, Forster Resonance Energy Transfer studies, but faces challenges in handling high flow rates. The innovative approach of combining time-resolved hardware with microfluidics and acoustics in an acoustofluidic time-resolved flow cytometer shows promise in balancing throughput with necessary optical signals for lifetime data.
FRONTIERS IN PHYSICS
(2021)
Article
Medicine, General & Internal
Alexey Borisov, Olga A. Zakharova, Alisa A. Samarinova, Natalia Yunusova, Olga Cheremisina, Yury Kistenev
Summary: This study aimed to investigate the applicability of exosome fluorescence-lifetime imaging microscopy (FLIM) for colorectal cancer (CRC) diagnosis. Exosomes were extracted using differential ultra-centrifugation from the blood plasma of patients with colon polyps and CRC. Analysis was performed using a two-photon FLIM device. The results showed that different classes of exosomes, differentiated by autofluorescence average lifetime tm, were predominantly found in samples from CRC and CP patients. The relative number of CRC-associated exosomes in the FLIM dataset differed significantly between the CP and CRC patient groups. Thus, FLIM has potential as a diagnostic tool for CRC.
Article
Multidisciplinary Sciences
Christine Terryn, Anouck Habrant, Gabriel Paes, Corentin Spriet
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Editorial Material
Chemistry, Multidisciplinary
Florent Allais, Xavier Coqueret, Thomas Farmer, Warwick Raverty, Caroline Remond, Gabriel Paes
FRONTIERS IN CHEMISTRY
(2020)
Review
Biotechnology & Applied Microbiology
Claire Bourlieu, Thierry Astruc, Sophie Barbe, Jean-Guy Berrin, Estelle Bonnin, Rachel Boutrou, Virginie Hugouvieux, Steven Le Feunteun, Gabriel Paes
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biochemistry & Molecular Biology
Saswat S. Mohapatra, Antonella Fioravanti, Pauline Vandame, Corentin Spriet, Francesco Pini, Coralie Bompard, Ralf Blossey, Odile Valette, Emanuele G. Biondi
MOLECULAR MICROBIOLOGY
(2020)
Article
Materials Science, Biomaterials
Franck Bouchart, Olivier Vidal, Jean-Marie Lacroix, Corentin Spriet, Shaan Chamary, Annie Brutel, Jean-Christophe Hornez
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Fabien Baldacci-Cresp, Julien Le Roy, Brigitte Huss, Cedric Lion, Anne Creach, Corentin Spriet, Ludovic Duponchel, Christophe Biot, Marie Baucher, Simon Hawkins, Godfrey Neutelings
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Angelina Kasprowicz, Corentin Spriet, Christine Terryn, Vincent Rigolot, Stephan Hardiville, Matthew G. Alteen, Tony Lefebvre, Christophe Biot
Article
Microbiology
Laura Riva, Corentin Spriet, Nicolas Barois, Costin-Ioan Popescu, Jean Dubuisson, Yves Rouille
Summary: This study investigates the dynamics and intracellular localization of the NS5A protein of hepatitis C virus, shedding light on its role in viral assembly rather than RNA replication. The findings contribute to a better understanding of the interplay between HCV proteins during the viral life cycle.
Article
Biotechnology & Applied Microbiology
Amandine Leroy, Xavier Falourd, Loic Foucat, Valerie Mechin, Fabienne Guillon, Gabriel Paes
Summary: Hot water pretreatment (HWP) alters the composition and structure of biomass, increasing saccharification kinetics and yield. Changes in cell wall composition, lignin structure, and cellulose environment after HWP contribute to enhanced accessibility of cellulases and promote hydrolysis. Understanding the interactions between polymers is crucial for optimizing HWP in sustainable biorefineries.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Claire Mayer-Laigle, Laurence Foulon, Clement Denoual, Miguel Pernes, Eric Rondet, Anthony Magueresse, Cecile Barron, Anouck Habrant, Alain Bourmaud, Gabriel Paes
Summary: The study successfully prepared reinforced filaments containing fluorescent flax particles through extrusion and 3D printing. Microstructural characterization showed that the fluorescent flax particles were homogeneously distributed in the 3D printed material. Despite the low content, a strong fluorescent emission could still be measured.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Multidisciplinary
Sacha Escamez, Christine Terryn, Madhavi Latha Gandla, Zakiya Yassin, Gerhard Scheepers, Torgny Nasholm, Ola Sundman, Leif J. Jonsson, Judith Lundberg-Felten, Hannele Tuominen, Totte Niittyla, Gabriel Paes
Summary: This study demonstrates the relationship between fluorescence lifetime and chemical composition of lignin, a major structural element in plant cell walls. By using FLIM and classical analytical methods, researchers accurately predicted the content of different lignin units in hybrid aspen trees with varying cell wall chemistry and structure. These findings provide insights into lignin biosynthesis dynamics and potential improvements for woody biomass feedstocks.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Agricultural Engineering
Amandine Leroy, Marie-Francoise Devaux, Mathieu Fanuel, Hugo Chauvet, Sylvie Durand, Camille Alvarado, Anouck Habrant, Christophe Sandt, Helene Rogniaux, Gabriel Paes, Fabienne Guillon
Summary: This work provides a dynamic perspective on the enzymatic degradation of maize cell walls and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Depending on their polymer composition and organization, different cell types exhibit varying degrees and rates of enzymatic degradation. Enzymes sequentially act from cell walls rich in accessible cellulose to the most recalcitrant cells, and this phenomenon may be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with cell type.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Julien du Pasquier, Gabriel Paes, Patrick Perre
Summary: This review critically analyzed the current state of dilute acid pretreatment applied to lignocellulosic biomass. Data from 63 publications were analyzed, and it was found that most studies used residence times of less than 30 minutes, temperature ranges from 100°C to 200°C, and acid levels between 0% and 2%. Yields were quantified by measuring xylose content or glucose content after enzymatic hydrolysis. Statistical analyses were performed to determine the time-temperature equivalence for three types of biomass, and non-linear expressions were used for formulation. Further research is recommended to explore less investigated areas, such as moderate temperature levels with longer residence times. Standardization and better description of pretreatment materials (time-temperature kinetics, reactor type) and analytical methods are necessary. The development of an open, findable, accessible, interoperable, and reusable data approach for pretreatment research is mandatory.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Edwige Audibert, Berangere Lebas, Corentin Spriet, Anouck Habrant, Brigitte Chabbert, Gabriel Paes
Summary: This study proposes an automated method for the quantification of chemical and morphological parameters through fluorescence macroscopy, which was applied on wood samples pretreated with steam explosion. The results show that steam explosion has a significant impact on the wood samples, altering the fluorescence intensity and causing morphological changes such as cell shrinkage and deformation of cell walls. The developed procedure allows simultaneous and effective quantification of morphological parameters and fluorescence intensity of the cell walls, providing valuable insights into biomass architecture.
Article
Oncology
Aurelien Haustrate, George Shapovalov, Corentin Spriet, Clement Cordier, Artem Kondratskyi, Lucile Noyer, Francois Foulquier, Natalia Prevarskaya, V'yacheslav Lehen'kyi
Summary: The upregulation of TRPV6 channel in cancer is associated with poor prognosis, but there is no reliable tool to target it. Two antibodies have been generated that can bind to TRPV6 and transiently activate calcium currents. This activation leads to apoptosis induction in prostate cancer cells, with only cells lacking TRPV6 expression being able to avoid death. Therefore, activating the TRPV6 channel may be more prospective than inhibiting it.