Journal
PLANT METHODS
Volume 9, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1746-4811-9-31
Keywords
Nuclei isolation; Solanaceae; Rosaceae; Tobacco; Potato; Apple
Categories
Funding
- European Social Fund under the Global Grant measure [VP1-3.1-SMM-07-K-01-041]
- Center of International Mobility (CIMO), Finland
- SS
- Academy of Finland [1253126]
Ask authors/readers for more resources
In this study, a protocol is described for rapid preparation of an enriched, reasonably pure fraction of nuclear proteins from the leaves of tobacco (Nicotiana tabacum), potato (Solanum tuberosum) and apple (Malus domestica). The protocol gives reproducible results and can be carried out quickly in 2 hours. Tissue extracts clarified with filtration were treated with non-ionic detergent (Triton X-100) to lyse membranes of contaminating organelles. Nuclei were collected from a 60% Percoll layer of density gradient following low-speed centrifugation. Western blot analysis using antibodies to marker proteins of organelles indicated that the nuclear protein fractions were highly enriched and free or nearly free of proteins from the endoplasmic reticulum and chloroplasts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available