Article
Plant Sciences
Emilie Kold Bredahl, Louise Kjaerulff, Chi Ndi, Susan Semple, Bevan Buirchell, Birger Lindberg Moller, Dan Staerk
Summary: This study investigated the crude extract of Eremophila spathulata leaves using different scales of high-performance liquid chromatography (HPLC) and hyphenated HPLC-photodiode array-high-resolution mass spectrometry-nuclear magnetic resonance (HPLC-PDA-HRMS-SPE-NMR), and discovered seven previously unreported caryophyllane sesquiterpenoids.
PHYTOCHEMISTRY LETTERS
(2022)
Article
Plant Sciences
Galaihalage K. S. Ananda, Sally L. Norton, Cecilia Blomstedt, Agnelo Furtado, Birger Lindberg Moller, Roslyn Gleadow, Robert J. Henry
Summary: Australian native species of sorghum contain negligible amounts of dhurrin in their leaves and the cyanogenesis process is regulated differently under water-stress in comparison to domesticated sorghum species. Cyanogenesis in forage sorghum is a major concern in agriculture as the leaves of domesticated sorghum are potentially toxic to livestock, especially at times of drought which induces increased production of the cyanogenic glucoside dhurrin. The wild sorghum species endemic to Australia have a negligible content of dhurrin in the above ground tissues and thus represent a potential resource for key agricultural traits like low toxicity.
Article
Genetics & Heredity
Max Cowan, Birger Lindberg Moller, Sally Norton, Camilla Knudsen, Christoph Crocoll, Agnelo Furtado, Robert Henry, Cecilia Blomstedt, Roslyn M. Gleadow
Summary: Domestication has caused a loss of genetic diversity in major food crops, but crop wild relatives could potentially provide novel genes for regaining climate resilience. Australian Sorghum wild species were found to be phenotypically acyanogenic, possibly due to their nutrient-poor environments. The transcriptional regulation of cyanogenic phenotype in wild sorghum is similar to that in elite sorghum.
Article
Biochemistry & Molecular Biology
Malene J. Petersen, Chao Liang, Louise Kjaerulff, Chi Ndi, Susan Semple, Bevan Buirchell, Sonia Coriani, Birger Lindberg Moller, Dan Staerk
Summary: Eremophila, a genus of Australian desert plants, has traditionally been used by Australian Aboriginal people for medicinal purposes. Extracts from Eremophila glabra showed inhibitory activity against alpha-glucosidase and protein tyrosine phosphatase 1B. The study identified new serrulatane diterpenoids and confirmed the presence of previously known compounds, some of which exhibited PTP1B inhibitory activities.
Article
Plant Sciences
Cecilie Cetti Hansen, Mette Sorensen, Matteo Bellucci, Wolfgang Brandt, Carl Erik Olsen, Jason Q. D. Goodger, Ian E. E. Woodrow, Birger Lindberg Moller, Elizabeth H. J. Neilson
Summary: This study investigates the biosynthesis of prunasin, a cyanogenic glucoside, in the economically and ecologically important genus Eucalyptus. By comparing cyanogenic and acyanogenic species, researchers identified a novel UDP-glucosyltransferase UGT87 that catalyzes the final step of prunasin biosynthesis. Additionally, the loss of different ortholog prunasin biosynthetic genes was observed in acyanogenic species. These findings highlight the heterogeneity and dynamic evolution of chemical defense pathways within a single genus, providing insights into the adaptability of Eucalyptus trees.
Article
Engineering, Environmental
Dan Zhao, Maria Louise Leth, Maher Abou Hachem, Iram Aziz, Natalija Jancic, Thomas Luxbacher, Claus Helix-Nielsen, Wenjing Zhang
Summary: Biocatalytic nanofibrous membranes have efficient catalytic performance and operational catalytic stability in wastewater treatment. The fabrication of flexible SiO2 biocatalytic nanofiber membranes and their application in emerging pollutant bioremediation have been reported. The membranes showed high enzyme immobilization yield and maintained residual activity after multiple cycles.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Maria Louise Leth, Michael Jakob Pichler, Maher Abou Hachem
Summary: Butyrate-producing human gut microbiota members are associated with a healthy immune-homeostasis and protection from inflammatory disorders and colorectal cancer. This is due to the production of butyrate, which is produced by prevalent and abundant bacteria in the gut. However, our understanding of the glycan utilization mechanisms and preferences of these bacteria is limited. Recent findings have revealed the strategies used by these bacteria to harvest energy from dietary fibers and human milk oligosaccharides (HMOs). The discovery of a conserved protein apparatus that enables the growth of butyrate producers on HMOs highlights their adaptation to both infant and adult guts.
ESSAYS IN BIOCHEMISTRY
(2023)
Article
Chemistry, Applied
Xiaoxiao Li, Yu Wang, Jing Wu, Zhengyu Jin, Lubbert Dijkhuizen, Maher Abou Hachem, Yuxiang Bai
Summary: In this study, five different starches were modified using enyzmes, and the thermo-reversibility of the modified starch gels was evaluated. The results showed that modified potato starch exhibited thermo-reversibility and improved stability. This suggests that modified potato starch has the potential to be used as a substitute for kappa-carrageenan and other hydrocolloids in food applications.
FOOD HYDROCOLLOIDS
(2023)
Article
Agronomy
Galaihalage K. S. Ananda, Sally L. Norton, Eve Barnes, Agnelo Furtado, Birger Lindberg Moller, Roslyn Gleadow, Robert J. Henry
Summary: In this study, the variation of grain size related genes in wild Sorghum was analyzed, revealing high morphological diversity in wild sorghum species. Six candidate genes related to grain size showed polymorphism, which may contribute to understanding and engineering grain size in sorghum and other cereals.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Chemistry, Analytical
Yong Zhao, Oliver Gericke, Tuo Li, Louise Kjaerulff, Kenneth T. Kongstad, Allison Maree Heskes, Birger Lindberg Moller, Flemming Steen Jorgensen, Henrietta Venter, Sonia Coriani, Susan J. Semple, Dan Staerk
Summary: The development of sustainable and safe drugs to combat emerging health pandemics requires new analytical technologies to explore the unique chemical properties in Nature. In this study, a new analytical technology called polypharmacology-labeled molecular networking (PLMN) was introduced, which combined positive and negative ionization tandem mass spectrometry with polypharmacological high-resolution inhibition profiling data. By applying PLMN analysis to the crude extract of Eremophila rugosa, the antihyperglycemic and antibacterial constituents were identified, revealing their activity in different assays. Furthermore, 27 new non canonical nerylneryl diphosphate-derived diterpenoids were discovered, with serrulatane ferulate esters showing potential antihyperglycemic and antibacterial activities, including synergy with oxacillin in methicillin-resistant Staphylococcus aureus strains and saddle-shaped binding to the active site of protein tyrosine phosphatase 1B. PLMN holds promise for polypharmacological natural-products-based drug discovery.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Chao Liang, Chi Ndi, Louise Kjaerulff, Susan Semple, Bevan Buirchell, Sonia Coriani, Birger Lindberg Moller, Dan Staerk
Summary: Extracts of Eremophila phyllopoda subsp. phyllopoda exhibited inhibitory activity against alpha-glucosidase and PTP1B. Through high-resolution profiling, 21 new serrulatane diterpenoids and five known furofuran lignans were identified. The structures were elucidated using HRMS and NMR spectroscopic data. The compounds showed potential as inhibitors for alpha-glucosidase and PTP1B.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Multidisciplinary Sciences
Bashar Shuoker, Michael J. Pichler, Chunsheng Jin, Hiroka Sakanaka, Haiyang Wu, Ana Martinez Gascuena, Jining Liu, Tine Sofie Nielsen, Jan Holgersson, Eva Nordberg Karlsson, Nathalie Juge, Sebastian Meier, Jens Preben Morth, Niclas G. Karlsson, Maher Abou Hachem
Summary: This study provides insights into the mechanism of mucin O-glycan degradation by Akkermansia muciniphila and the nutrient sharing between this symbiotic bacteria and the mucus-associated microbiota. They discovered new enzymes involved in the breakdown of protective caps on mucin, which promote the growth of A. muciniphila and the production of butyrate through cooperation with other bacteria.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yong Zhao, Nikolaj L. Hansen, Yao-Tao Duan, Meera Prasad, Mohammed S. Motawia, Birger L. Moller, Irini Pateraki, Dan Staerk, Soren Bak, Karel Miettinen, Sotirios C. Kampranis
Summary: Researchers have identified a compound called celastrol in the roots of Tripterygium wilfordii, which has potential as an effective treatment for obesity. They have also developed a synthetic method for celastrol, allowing it to be produced from table sugar. This study demonstrates the successful combination of plant biochemistry, metabolic engineering, and chemistry for the scalable synthesis of complex specialized metabolites.
Article
Plant Sciences
Chao Liang, Chi Ndi, Louise Kjaerulff, Susan Semple, Bevan Buirchell, Sonia Coriani, Birger Lindberg Moller, Dan Staerk
Summary: Extracts of Eremophila phyllopoda subsp. phyllopoda demonstrated inhibitory activity against alpha-glucosidase and PTP1B. Through extensive analysis, 21 previously undescribed serrulatane diterpenoids, two known serrulatane diterpenoids, and five known furofuran lignans were identified. These compounds showed potential as inhibitors against alpha-glucosidase and PTP1B.
JOURNAL OF NATURAL PRODUCTS
(2023)
Meeting Abstract
Biophysics
Nikos S. Hatzakis, Simon B. Jensen, Sara Thodberg, Shaheena Parween, Matias E. Moses, Cecillie C. Hansen, Camilla Knudsen, Rita D. Giudice, Philip M. Lund, Patricia Castano, Yanet G. Bustamante, Flemming S. Jorgennsen, Amit V. Pandey, Tomas Laursen, Birger L. Moller
BIOPHYSICAL JOURNAL
(2022)