Journal
PLANT JOURNAL
Volume 81, Issue 1, Pages 40-52Publisher
WILEY
DOI: 10.1111/tpj.12691
Keywords
importin-alpha; nucleo-cytoplasmic transport; nuclear localization sequence; oomycete effector; plant innate immunity; Hyaloperonospora arabidopsidis; Arabidopsis thaliana
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Funding
- BBSRC-UK [BBJ00453, BBK009176]
- German Research Foundation (DFG)
- BBSRC [BB/K009176/1, BBS/E/J/000C0624] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000C0624] Funding Source: researchfish
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Importin-alpha s are essential adapter proteins that recruit cytoplasmic proteins destined for active nuclear import to the nuclear transport machinery. Cargo proteins interact with the importin-alpha armadillo repeat domain via nuclear localization sequences (NLSs), short amino acids motifs enriched in Lys and Arg residues. Plant genomes typically encode several importin-alpha paralogs that can have both specific and partially redundant functions. Although some cargos are preferentially imported by a distinct importin-alpha it remains unknown how this specificity is generated and to what extent cargos compete for binding to nuclear transport receptors. Here we report that the effector protein HaRxL106 from the oomycete pathogen Hyaloperonospora arabidopsidis co-opts the host cell's nuclear import machinery. We use HaRxL106 as a probe to determine redundant and specific functions of importin-alpha paralogs from Arabidopsis thaliana. A crystal structure of the importin-alpha 3/MOS6 armadillo repeat domain suggests that five of the six Arabidopsis importin-alpha s expressed in rosette leaves have an almost identical NLS-binding site. Comparison of the importin-alpha binding affinities of HaRxL106 and other cargos in vitro and in plant cells suggests that relatively small affinity differences in vitro affect the rate of transport complex formation in vivo. Our results suggest that cargo affinity for importin-alpha, sequence variation at the importin-alpha NLS-binding sites and tissue-specific expression levels of importin-alpha s determine formation of cargo/importin-alpha transport complexes in plant cells.
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