Article
Multidisciplinary Sciences
Yuan Xu, Thomas Wieloch, Joshua A. M. Kaste, Yair Shachar-Hill, Thomas D. Sharkey
Summary: This study presents a carbon-13 washout model for leaf metabolism and identifies three processes that contribute to the labeling of Calvin-Benson cycle (CBC) metabolites. The model integrates CBC, cytosolic sugar pools, the glucose-6-phosphate shunt, and vacuolar sugars into a single system, providing a more complete understanding of carbon movement in photosynthetic metabolism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Jianqiang Mu, Yajuan Fu, Bucang Liu, Yao Zhang, Aiying Wang, Yuxia Li, Jianbo Zhu
Summary: Saussurea involucrata is a cold-resistant plant species that grows in extreme arctic conditions. The key enzyme Fructose-1, 6-bisphosphate aldolase (FBA) plays a crucial role in the photosynthesis process. A cold-responsive gene, SiFBA5, has been identified and shown to positively regulate plant response to cold stress, offering potential for improving crop yield under cold stress conditions.
Article
Plant Sciences
Komal Pandey, Ravi Shankar Kumar, Priti Prasad, Veena Pande, Prabodh Kumar Trivedi, Pramod Arvind Shirke
Summary: The study found that under water stress, photosynthesis decreases in guar varieties, leading to reduced CO2 assimilation and increased oxidative stress with accumulation of antioxidant enzymes. Certain varieties showed higher ROS scavenging capacity under water stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Chong Chen, Meng Zhang, Xiaocui Ma, Qingwei Meng, Kunyang Zhuang
Summary: This study reveals that the expression patterns and thermostabilities of SlTPI1 and SlTPI2 in tomato differ under heat stress, which could be applied to enhance thermotolerance in crops.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Xiaowei Yin, Mingjia Tang, Xiaojian Xia, Jingquan Yu
Summary: In this study, the researchers investigated the role of brassinosteroids (BRs), a type of plant hormone, in regulating the Calvin cycle genes in tomatoes. They found that BRs can enhance the activity of the Calvin cycle and improve photosynthetic ability. This regulation is mediated by the signal effector BRASSINAZOLE RESISTANT 1 (BZR1), which directly binds to the promoters of key Calvin cycle genes. These findings suggest that BRs regulate the Calvin cycle in a BZR1-dependent manner in tomatoes, highlighting the potential of targeting BRs to increase crop yields.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Engineering, Environmental
Xinru Zhang, Na Liu, Huijie Lu, Lizhong Zhu
Summary: This study investigated the mechanism of carbon fixation disturbance in rice caused by organic pollutants, revealing that the pollutants downregulated the activity and gene expression of enzymes involved in carbon fixation, leading to biomass reduction. Molecular simulation suggested that the pollutants potentially competed with CO2 for binding to active sites, reducing CO2 capture efficiency.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Jingyi Hu, Jie Chen, Wei Wang, Lizhong Zhu
Summary: PAHs pollution inhibits rice growth and photosynthesis by downregulating chlorophyll synthesis genes, accelerating degradation processes and disturbing chlorophyll metabolism, leading to a decrease in carbon fixation and plant biomass.
Review
Cell Biology
P. Lemonnier, T. Lawson
Summary: Stomatal conductance plays a crucial role in determining CO2 uptake and water loss in plants, affecting overall water status and productivity. However, the signals coordinating mesophyll demands for CO2, the role of chloroplasts in stomatal function, and other GC metabolic processes in stomatal function remain poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Article
Plant Sciences
Ying Liu
Summary: The study reveals that grazing rest during the spring regreening period can promote the ecological restoration of degraded alpine meadows by enhancing the photosynthesis and respiration of dominant grass species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Gian Luca Borghi, Stephanie Arrivault, Manuela Guenther, David Barbosa Medeiros, Emilia Dell'Aversana, Giovanna Marta Fusco, Petronia Carillo, Martha Ludwig, Alisdair R. Fernie, John E. Lunn, Mark Stitt
Summary: Metabolite profiling and (13)CO(2)labelling studies of Flaveria species reveal the progressive re-wiring of various metabolic processes in the evolution from C(3)to C(4)photosynthesis. This includes the relocation of enzymes, establishment of shuttles, and changes in the concentration of organic acids. The findings support current models and provide further insights into the evolutionary path of C(4)photosynthesis in the genus Flaveria.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Min Xu, Xiaomin Tian, Tingting Ku, Guangyuan Wang, Enying Zhang
Summary: Lysine malonylation is an important post-translational modification that plays a regulatory role in maize. The study provided a comprehensive survey of the malonylome in maize, revealing its essential regulatory role in photosynthesis and carbon fixation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yonghong Li, Lianwei Peng, Xiaoqin Wang, Lin Zhang
Summary: This study identifies the critical role of chloroplast ribulose-5-phosphate-3-epimerase (RPE) in the Calvin-Benson cycle and its influence on the photosynthetic capacity of chloroplasts.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Christine A. Raines
Summary: The Calvin-Benson-Bassham (CBB) cycle is the most important pathway on earth, capturing and converting CO2 into organic molecules for the basis of life. This cycle has been extensively studied and is highly conserved in nature. The enzymes in this cycle regenerate the CO2 acceptor molecule RuBP. Manipulating RuBP regeneration has the potential to improve photosynthesis and increase crop yields.
Article
Biochemistry & Molecular Biology
Helene Launay, Hui Shao, Olivier Bornet, Francois-Xavier Cantrelle, Regine Lebrun, Veronique Receveur-Brechot, Brigitte Gontero
Summary: In the chloroplast, the CP12 protein regulates the activity of Calvin-Benson-Bassham enzymes through redox transitions, with its structure detected through NMR and mass-spectrometry techniques, and validated in a cell extract. The different oxidation rates of the two cysteine pairs confirm the important role of CP12 in regulation.
Review
Cell Biology
Thomas D. Sharkey
Summary: The pentose phosphate pathway (PPP) consists of an oxidative branch and a non-oxidative branch, with the latter playing a crucial role in the Calvin-Benson cycle. The non-oxidative branch can convert CO2 into sugar through metabolic reversal, involving reactions that consume ATP in photosynthesis. The oxidative branch forms a shunt with the non-oxidative branch, leading to an unusual labeling pattern in the cytosol and a potential stress-induced response in the stroma.
Review
Biochemistry & Molecular Biology
Elisabeth Kurze, Matthias Wuest, Jieren Liao, Kate McGraphery, Thomas Hoffmann, Chuankui Song, Wilfried Schwab
Summary: Research has shown that the glycosylation of terpenoids can alter their physiological activities and this reaction remains chemically challenging. Scientists have successfully conducted functional studies on various plant enzymes involved in glycosylation, providing crucial clues for better understanding the mechanism of terpenoid glycosylation.
NATURAL PRODUCT REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Agriculture, Multidisciplinary
Francesca Zamolo, Matthias Wuest
Summary: The biosynthesis of IBMP in bell pepper fruits was investigated using stable isotope-labeled precursors. It was found that IBMP is synthesized from specific precursors in the pericarp tissues of unripe bell pepper fruits but not in the pericarp tissues of ripe bell pepper fruits.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Amber N. Parrish, Iris Lange, Dunja Samec, Bernd Markus Lange
Summary: This study analyzed the chemical diversity in three species of the genus Equisetum. The results showed that there were species- and organ-specific patterns of metabolite accumulation. Different metabolites contributed to the separation of shoot and rhizome samples. The findings provide a foundation for further investigation into the metabolism of this ancient land plant lineage.
Article
Biochemistry & Molecular Biology
Francesca Zamolo, Jan Phillip Heinrichs, Matthias Wuest
Summary: This study examined the distribution, translocation, and biosynthetic sites of IBMP in bell pepper plants. The results showed that IBMP was the most abundant pyrazine in all aerial plant parts, with IPMP being dominant in root tissues. The highest levels of IBMP were found in unripe bell pepper fruits. Biosynthesis of IBMP was observed in all plant parts, but not in roots and ripe bell pepper fruits. There was no evidence of phloem translocation of IBMP.
Article
Chemistry, Multidisciplinary
Francesca Zamolo, Matthias Wuest
Summary: 3-Isobutyl-2-methoxypyrazine (IBMP) is a highly potent odorant that gives fruits and vegetables their distinct aroma. Bell peppers, in particular, have high levels of IBMP, making it the compound responsible for their characteristic smell. However, the biosynthesis of methoxypyrazines, including IBMP, has been unknown until now. Through feeding experiments with stable-isotope labeled precursors, it has been discovered that l-Serine plays a key role in the construction of the pyrazine ring in 3-alkyl-2-methoxypyrazines (MPs). Analysis using HS-SPME-GCxGC-TOF-MS revealed that the biosynthetic pathway to IBMP is closely linked to l-Serine derived from photorespiration.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemical Research Methods
Jan Leppert, Tillman Brehmer, Matthias Wuest, Peter Boeker
Summary: A fast and reliable method is introduced to evaluate retention parameters for gas chromatography based on the distribution-centric 3-parameter model. The method uses a differentiable model of solute migration in a gas chromatographic system and employs Newton's method with trust region to determine the parameters. The determined retention parameters can be applied in method development and show good agreement with isothermal GC measurements.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Plant Sciences
B. Markus Lange, Narayanan Srividya
Summary: In this article, the authors review all functionally characterized monoterpene synthases (MTSs) and sesquiterpene synthases (STSs) of Cannabis sativa L. They also explore the locations of MTSs and STSs in the chromosome-level assembly of the reference chemovar CBDRx. Computational analyses and literature reviews are combined to lay the foundation for future studies on the determinants of substrate and product specificity in these enzymes.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Plant Sciences
B. Markus Lange, Narayanan Srividya, Iris Lange, Amber N. Parrish, Lukas R. Benzenberg, Iovanna Pandelova, Kelly J. Vining, Matthias Wuest
Summary: The essential oils extracted from above-ground materials of mint family members are widely used in consumer products. Previous research focused on the characterization of terpenoid oil constituents in mint leaves, but we found that other organs, such as stems, rhizomes, and roots, also emit volatiles with varying compositions. The analysis of volatile quantities released by mint plants treated with Verticillium dahliae-spore suspension or water showed differences in susceptible and resistant species, indicating their involvement in disease resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Tillman Brehmer, Benny Duong, Manuela Marquart, Luise Friedemann, Peter J. Faust, Peter Boeker, Matthias Wuest, Jan Leppert
Summary: This study presents an open source database with suitable retention parameters for prediction and simulation of GC separations, and introduces three common retention models. Useful computer simulations are important for saving resources and time in GC method development. The standardized procedure of measurements and calculations presented in this study is beneficial for chromatographers, analytical chemists, and method developers. The main benefits include simulations of temperature-programed GC separations and comparison with measurements, with observed deviation of predicted retention times being less than 1%. The database includes over 900 entries covering a wide range of compounds on more than 20 different GC columns, such as VOCs, PAHs, FAMEs, PCBs, or allergenic fragrances.
Review
Biochemistry & Molecular Biology
Francesca Zamolo, Matthias Wuest
Summary: Pyrazines are widely present in nature and are synthesized by microorganisms, insects, and plants. They have diverse biological functions, including playing a role as semiochemicals and aroma compounds in foods. 3-alkyl-2-methoxypyrazines (MPs) have been the focus of research due to their association with green and earthy attributes and their influence on the aroma of vegetables and wines. The biosynthetic pathway and precursors of MPs have been the subject of discussion and were recently discovered to involve l-leucine and l-serine as important precursors for IBMP.
Article
Agriculture, Multidisciplinary
Marvin Benedikt Brenner, Sandra Flory, Matthias Wuest, Jan Frank, Karl Wagner
Summary: Currently, there is a lack of in vitro dissolution methods that can accurately predict the in vivo bioavailability of compounds from complex mixtures. This study developed an in vivo predictive dissolution method (BiPHa+) using data on the in vivo performance of bioavailability-improved curcumin formulations. Compared with conventional dissolution systems, BiPHa+ demonstrated superior in vivo-relevant formulation characterization and had the highest degree of linearity in predicting bioavailability. It also provided mechanistic information about formulation-dependent release characteristics.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Tillman Brehmer, Peter Boeker, Matthias Wuest, Jan Leppert
Summary: The development of new analytical methods that support the optimization process can save resources, time, and costs. In this study, the relationship between the measured elution temperature and the characteristic temperature was investigated for various volatile substances. A novel multivariate curve fit model was developed, which accurately describes the relation between the characteristic temperature T-char and elution temperatures T-elu, expanding the prediction range for high volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Horticulture
Charlotte Hubert, Saskia Tsiaparas, Liane Kahlert, Katharina Luhmer, Marcel Dieter Moll, Maike Passon, Matthias Wuest, Andreas Schieber, Ralf Pude
Summary: This study investigated the effects of two different postharvest processes on the content and composition of essential oils in three different Mentha genotypes. The results showed that the postharvest processing treatments did not significantly affect the essential oil content and composition, but genotype selection and harvest time had a significant impact.
Article
Plant Sciences
Koichi Sugimoto, Jordan J. Zager, Brian St Aubin, Bernd Markus Lange, Gregg A. Howe
Summary: The deficiency of flavonoids in glandular trichomes of tomato leads to an increase in the accumulation of reactive oxygen species and downregulation of terpenoid biosynthesis in a specific cell type.