Review
Agronomy
Agnieszka Kielkowska, Waldemar Kiszczak
Summary: This paper presents the historical background and current status of haploid induction in carrot, and discusses the potential of haploidization technology in carrot breeding. The methods of androgenesis, gynogenesis, and induced parthenogenesis, as well as the manipulation of the centromere-specific histone 3 variant (CENH3) in carrot, are also acknowledged.
Article
Plant Sciences
Karen Cristina Fialho dos Santos, Antonio da Silva Souza, Carlos Alberto da Silva Ledo, Leila Vasconcelos Costa Nobre, Marcus Dhilermando Hora de Souza, Denise dos Santos Vila Verde, Walter dos Santos Soares Filho
Summary: This study aimed to refine the protocols for callus induction in different species of Citrus and Poncirus. Only the Citrus medica L. genotype responded to the treatments and was subjected to callus multiplication. Other genotypes are still under investigation.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Eva Nagy, Timea Szaloki, Janos Pauk
Summary: Doubled haploid (DH) plant production, such as anther culture (AC), is a useful tool in modern rice breeding. This study compared the effects of different combinations of plant growth regulators on callus induction in rice, and identified the best combination for in vitro green plant production.
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Timea Szaloki, Shoba Venkatanagappa, Janos Pauk
Summary: An efficient protocol for in vitro anther culture in rice breeding, especially for indica genotypes, was evaluated. Three different induction media (N6NDK, N(6)NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested to increase the efficiency of in vitro androgenesis. The production of calli and green plantlets varied among different genotypes and media combinations. Flow cytometric analyses showed that 51.89% of the acclimatized indica rice plantlets had undergone spontaneous chromosome doubling. The utilization of DH plants has been integrated into the Hungarian rice breeding program, and tetraploid lines offer potential for future research and breeding directions.
Article
Plant Sciences
Katarzyna Marciniak, Krzysztof Przedniczek
Summary: This study demonstrated the specific changes in the yellow lupine anther structure during dehiscence, and identified genes associated with this process, showing that their transcription is highly coexpressed and regulated in a GA-dependent manner. The localization of GA(3) in septum cells, near the vascular bundle, and endothecium before anther opening suggests its role in regulating gene expression related to GA biosynthesis and deactivation. The results provide valuable insights into the hormonal and molecular regulation of anther dehiscence in yellow lupine, offering potential applications in breeding for improved fertility in this economically important crop species.
Article
Biology
Jozsef Kruppa, Osama Zuhair Kanbar, Kitti Andrea Toth-Lencses, Erzsebet Kiss, Lajos Bona, Csaba Lantos, Janos Pauk
Summary: In triticale anther cultures, the efficiency of androgenesis is significantly influenced by genotype and induction medium. Both W14mf and P4mf medium are suitable for producing triticale DH plants, with P4mf medium showing higher efficiency. Breeders should be cautious about the outcross character of triticale and ensure careful propagation and maintenance.
Article
Plant Sciences
Stanislava Grozeva, Gancho Pasev, Vesela Radeva-Ivanova, Velichka Todorova, Valentina Ivanova, Amol N. Nankar
Summary: This study evaluated the androgenic potential of 180 pepper accessions and 11 progenies with PMMoV resistance. Results revealed genotype-dependent embryo induction with differences in regenerated plants among different varietal groups. Use of anther culture will facilitate genetic improvement in ongoing pepper breeding programs.
Article
Agriculture, Multidisciplinary
Stefan Ritter, Martina Gastl, Thomas Becker
Summary: Lupines and faba beans, protein-rich legumes, can be used as a plant-based alternative to animal proteins in the beverage industry. However, their usability is hindered by low protein solubility and the presence of antinutrients. This study found that germination can enhance protein solubility, increase levels of free amino acids, and decrease the concentration of antinutrients in lupines and faba beans. These findings demonstrate the potential of germination as a process for refining these legumes for various food applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Elena Lima-Cabello, Julia Escudero-Feliu, Andreina Peralta-Leal, Pedro Garcia-Fernandez, Kadambot H. M. Siddique, Karam B. Singh, Maria I. Nunez, Josefa Leon, Jose C. Jimenez-Lopez
Summary: Narrow-leafed lupin (NLL) beta-5 and beta-7 conglutin proteins, containing unique structural features like the mobile arm at the N-terminal, showed anti-inflammatory and oxidative cell state regulatory properties, reducing pro-inflammatory mediators and cytokines. Truncated forms of these proteins did not exhibit the same effects. This suggests that the mobile arm domain is key in developing nutraceutical properties, making NLL beta-5 and beta-7 potential functional food components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Kseniya Belova, Elena Dushina, Sergey Popov, Andrey Zlobin, Ekaterina Martinson, Fedor Vityazev, Sergey Litvinets
Summary: The study aims to develop and evaluate the printability of k-carrageenan inks enriched with lupin callus tissue and examine their effect on the texture and digestibility of 3D-printed gel. Results showed successful 3D printing of enriched ink at specific concentrations of callus. Higher concentrations of callus material reduced the feasibility of 3D printing. The texture and rheological properties of the gel were influenced by the type and concentration of callus used. Incorporating lupin callus into hydrocolloid ink for food 3D printing could lead to the development of gelling material with new properties.
Article
Biotechnology & Applied Microbiology
Csaba Lantos, Szabolcs Lehoczki-Krsjak, Janos Pauk
Summary: This study compared different pre-treatments to investigate the effects of stress pre-treatments on the efficiency of androgenesis in einkorn. It successfully induced androgenesis and regenerated green and albino plantlets in in vitro anther culture of einkorn. Flow cytometric analyses confirmed the ploidy level of the microspore-derived green plantlets as haploid.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Agronomy
Ade Mulyana, Bambang S. Purwoko, Iswari S. Dewi, Awang Maharijaya
Summary: Eggplant is a highly produced and valuable vegetable crop. Anther culture is used to produce pure lines for hybrid breeding. This study compared six anther culture methods and found that the Modification 1 method was the most effective in producing in vitro androgenic regenerated plants in eggplant. The ploidy level of regenerants was confirmed using flow cytometry.
Article
Plant Sciences
Monika Gajecka, Marek Marzec, Beata Chmielewska, Janusz Jelonek, Justyna Zbieszczyk, Iwona Szarejko
Summary: This study investigated the genotype-dependent molecular differences in chloroplast differentiation leading to the formation of green and albino regenerants in microspore culture of barley. The results showed that the failed transition from NEP-dependent to PEP-dependent transcription in plastids was associated with albino regenerants, while a high activity of PEP polymerase and increased expression of certain genes correlated with the production of green regenerants. Understanding these molecular mechanisms may help overcome the issue of albinism in cereal androgenesis.
Article
Plant Sciences
Abdullah, Furrukh Mehmood, Iram Shahzadi, Zain Ali, Madiha Islam, Muhammad Naeem, Bushra Mirza, Peter J. Lockhart, Ibrar Ahmed, Mohammad Tahir Waheed
Summary: This study explored the correlations among mutational events in chloroplast genomes of species in the Malvaceae family, finding that oligonucleotide repeats can serve as a proxy for identifying mutational hotspots. The extent of correlations between InDels and single-nucleotide polymorphism, InDels and repeats, as well as SNPs and repeats ranged from weak to moderate. At different taxonomic levels, a high percentage of repeats showed co-occurrence with SNPs, indicating a potential relationship between these mutational events.
JOURNAL OF SYSTEMATICS AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Abdullah, Claudia L. Henriquez, Furrukh Mehmood, Monica M. Carlsen, Madiha Islam, Mohammad Tahir Waheed, Peter Poczai, Thomas B. Croat, Ibrar Ahmed
JOURNAL OF MOLECULAR EVOLUTION
(2020)
Article
Multidisciplinary Sciences
Furrukh Mehmood, Abdullah, Zartasha Ubaid, Iram Shahzadi, Ibrar Ahmed, Mohammad Tahir Waheed, Peter Poczai, Bushra Mirza
Article
Plant Sciences
Abdullah, Claudia L. Henriquez, Furrukh Mehmood, Iram Shahzadi, Zain Ali, Mohammad Tahir Waheed, Thomas B. Croat, Peter Poczai, Ibrar Ahmed
Article
Biotechnology & Applied Microbiology
Abdullah, Claudia L. Henriquez, Furrukh Mehmood, Amir Hayat, Abdul Sammad, Shahid Waseem, Mohammad Tahir Waheed, Peter J. Matthews, Thomas B. Croat, Peter Poczai, Ibrar Ahmed
Summary: The study elucidated the molecular evolution in cp genomes of species in the Dracunculus clade, revealing high similarity in gene content and genetic characteristics across different genera. It also identified suitable polymorphic loci for phylogenetic inference, broadening our understanding of the chloroplast genome evolution in aroids.
Article
Biochemistry & Molecular Biology
Sara Latif, Johanna Gottschamel, Tahira Syed, Iqra Younus, Kehkshan Gull, Muhammad Sameeullah, Neelam Batool, Andreas Guenter Loessl, Filipe Mariz, Martin Mueller, Bushra Mirza, Mohammad Tahir Waheed
Summary: This study successfully expressed the HPV-16 L1 gene in tobacco chloroplasts, induced L1 protein production using an ethanol-inducible promoter, and purified the protein. The transplastomic tobacco plants developed normally, produced viable seeds and pollen, confirming the use of inducible expression as a safe approach for expressing transgenes.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Sandra Rychel-Bielska, Anna Surma, Wojciech Bielski, Bartosz Kozak, Renata Galek, Michal Ksiazkiewicz
Summary: White lupin is a pulse annual plant with high-protein grain, showing diverse flowering time control mechanisms and suggesting the need for genome-wide association studies for future research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jacek Lyczko, Jacek Piotr Twardowski, Bartlomiej Skalny, Renata Galek, Antoni Szumny, Iwona Gruss, Dariusz Piesik, Sebastian Sendel
Summary: Rare carnivorous plants of the genus Sarracenia are highly interesting to scientists in various fields due to their unique capacity to attract insects. This study focused on developing a micropropagation protocol for Sarracenia alata and identifying its specific chemical composition of volatile compounds. The research confirmed the presence of major volatile compounds responsible for attracting insects and showed the potential for cultivating rare carnivorous plants in vitro for bioactive compounds.
Article
Biochemistry & Molecular Biology
Muhammad Sameeullah, Muhammet Yildirim, Noreen Aslam, Mehmet Cengiz Baloglu, Buhara Yucesan, Andreas G. Loessl, Kiran Saba, Mohammad Tahir Waheed, Ekrem Gurel
Summary: The study revealed the important role of 3β-HSD, P5β R1, and P5β R2 genes in enhancing salt tolerance under saline stress, with the improvement in biomass and chlorophyll content observed in transplastomic tobacco plants through plastid genetic engineering. The transgenic lines also showed increased levels of metabolites (such as sucrose, glutamate, proline) and a lower Na+/K+ ratio, suggesting their involvement in salt stress management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Inese Kokina, Ilona Plaksenkova, Renata Galek, Marija Jermalonoka, Elena Kirilova, Vjaceslavs Gerbreders, Marina Krasovska, Eriks Sledevskis
Summary: This research found that Fe3O4 NPs at low concentrations can be successfully used as nanonutrition to increase barley photosynthetic efficiency and yield, while also observing an increase in genotoxicity. Future studies are needed to further investigate the impact of these NPs on gene expression in treated barley seedlings.
Article
Biochemistry & Molecular Biology
Noreen Aslam, Muhammad Sameeullah, Muhammet Yildirim, Mehmet Cengiz Baloglu, Buhara Yucesan, Andreas G. Lossl, Mohammad Tahir Waheed, Ekrem Gurel
Summary: Although members of the SDR gene family are widely distributed in different kingdoms of life, their roles in gene expression and regulation under stress conditions are not fully understood. In this study, we isolated and functionally characterized the 3 beta-HSD promoter from Digitalis ferruginea and examined its expression in Arabidopsis thaliana. The promoter showed stable expression under different abiotic stress conditions and responded to methyl jasmonate application. These findings suggest that the 3 beta-HSD promoter could be a potential candidate for the development of transgenic plants, particularly under changing environmental conditions.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Agronomy
Michal Dziwak, Katarzyna Wroblewska, Antoni Szumny, Renata Galek
Summary: Bryophytes are a diverse group of plants with important ecological functions. They produce secondary metabolites that help defend against herbivores and pathogens, as well as exhibit stress tolerance properties. Bryophytes have been used in various cultures for health purposes, and scientists are discovering new compounds with potential applications.
Article
Biochemistry & Molecular Biology
Maciej Jakobina, Jacek Lyczko, Kinga Zydorowicz, Renata Galek, Antoni Szumny
Summary: This study analyzed the volatile compounds in the stem of pitaya using the SPME-GC-MS technique and found that the addition of BA and IAA as plant growth regulators in the culture medium can affect the content and composition of volatile compounds. The results indicate that the pitaya stem contains a rich variety of volatile compounds, and plant hormones can modify their profile.
Article
Multidisciplinary Sciences
Sylwia Lewandowska, Katarzyna Dziergowska, Renata Galek, Izabela Michalak
Summary: The effect of extract obtained by Ultrasound-Assisted Extraction (UAE) from Cladophora glomerata on the germination and early growth of three narrow-leaved lupin varieties was investigated. Results showed that the concentration of the extract significantly influenced the growth of seedlings, with the 20% extract demonstrating better stimulation effect on all lupin cultivars.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Multidisciplinary
Ayisha Zia, Misbah Zahoor, Abdullah Abdullah, Asma Nisar, Neelam Batool, Amana Bibi, Kiran Saba, Ibrar Ahmed, Arzu Karatas, Ekrem Gurel, Mohammad Tahir Waheed
Summary: This study sequenced and analyzed the chloroplast genomes of two cultivars of broccoli, revealing highly similar genome structures and providing insights into gene encoding frequencies and repeat sequences. The findings will contribute to a better understanding of the chloroplast genomes of broccoli and serve as a resource for chloroplast genetic engineering.
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
(2022)