Article
Horticulture
Rachidatou Sikirou, Marie Epiphane Dossoumou, Judith Honfoga, Victor Afari-Sefa, Ramasamy Srinivasan, Mathews Paret, Wubetu Bihon
Summary: Bacterial wilt caused by Ralstonia solanacearum is a growing concern in amaranth production in Benin. Host resistance is identified as the most sustainable disease control measure. The study suggests that the UG-AMES13-2 variety from the World Vegetable Center shows high resistance to R. solanacearum and should be promoted for seed production to support farmers.
Article
Multidisciplinary Sciences
Dingxin Wen, Qingqing Guo, Wan Zhao, Yong Yang, Chunlei Yang, Jun Yu, Yun Hu
Summary: The study found that hydrogen sulfide can prevent tobacco bacterial wilt. The results showed that hydrogen sulfide can inhibit the growth of the bacteria, alter its cell morphology, inhibit biofilm formation and swarming motility, and reduce the population of bacteria invading tobacco roots.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Keming Yang, Xiaofang Wang, Rujiao Hou, Chunxia Lu, Zhe Fan, Jingxuan Li, Shuo Wang, Yangchun Xu, Qirong Shen, Ville-Petri Friman, Zhong Wei
Summary: This study investigates the influence of rhizosphere phage communities on soil suppressiveness and bacterial wilt disease. It shows that healthy plants are associated with phage communities that have stronger control over the pathogen R. solanacearum. Additionally, phages that target pathogen-inhibiting bacteria are more abundant in diseased plant microbiomes. These findings suggest that rhizosphere phage communities play a crucial role in determining bacterial wilt disease outcomes and soil suppressiveness.
Article
Plant Sciences
Erika Valente de Medeiros, Neyla Thayna Lima, Jose Romualdo de Sousa Lima, Kedma Maria Silva Pinto, Diogo Paes da Costa, Rafaela Felix da Franca, Cicero Luiz Franco Junior, Gustavo Pereira Duda, Antonio Celso Dantas Antonino, Claude Hammecker
Summary: The study demonstrates the potential value of biochar in managing tomato diseases, showing that biochar from different sources can inhibit the growth of R. solanacearum and reduce the severity of bacterial wilt in tomatoes.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
Wenlong Liang, Jingli Cheng, Jiadong Zhang, Qiuyu Xiong, Maojun Jin, Jinhao Zhao
Summary: By utilizing a core-shell nanocarrier that releases the bactericide berberine in an acidic environment, this study demonstrates an effective control of tomato bacterial wilt disease without negative impact on plant growth.
Article
Agriculture, Multidisciplinary
Keke Dang, Jinfeng Hou, Hong Liu, Junwei Peng, Yang Sun, Jiangang Li, Yuanhua Dong
Summary: In this study, the changes in rhizobacteria, endobacteria, and root exudates during the pathogenesis of bacterial wilt in ginger caused by Ralstonia solanacearum (Rs) were analyzed using high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). The results showed that Rs infection led to a 50.00% incidence of bacterial wilt in ginger and altered the composition of bacterial communities in both the endosphere and rhizosphere. Furthermore, the changes in rhizobacterial composition induced corresponding changes in endobacterial and root exudate compositions. The upregulated exudates inhibited ginger bacterial wilt, indicating that ginger produces antibacterial compounds as a defense mechanism against bacterial pathogens.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Dhananjay Kumar Yadav, Venkatappa Devappa, Abhijeet Shankar Kashyap, Narendra Kumar, V. S. Rana, Kumari Sunita, Dinesh Singh
Summary: Chemical mutagenic agents and ultraviolet irradiation were used to enhance the antagonistic property of Bacillus amyloliquefaciens DSBA-11 against Ralstonia solanacearum UTT-25 for managing tomato wilt disease effectively. The derivative strain MHNO2-20, treated with nitrous acid, showed the highest inhibition to R. solanacearum UTT-25. GC/MS analysis detected 18 major compounds in MHNO2-20, with compound 3-isobutyl hexahydropyrrolo (1,2), pyrazine-1,4-dione being the most abundant. Bio-efficacy assessment revealed that MHNO2-20 had the highest control efficacy (88.75%) against bacterial wilt disease in tomato plants.
Article
Chemistry, Multidisciplinary
Ying Wang, Yusen Li, Xin Zhou, Wenna Zhang, Shusheng Zhang, Dongmei Xi
Summary: A rapid and purification-free alpha-hemolysin nanopore-sensing strategy based on PCR and lambda exonuclease digestion was established to detect R. solanacearum, the soil-borne bacterium causing tobacco bacterial wilt. The sensing approach can be conveniently extended to other DNA monitoring due to the wide range of PCR applications.
Article
Biotechnology & Applied Microbiology
Qian Zhao, Jianmin Cao, Xianjie Cai, Jie Wang, Fanyu Kong, Dongkun Wang, Jing Wang
Summary: Volatile organic compounds (VOCs) produced by beneficial bacteria can inhibit the growth of plant-pathogenic microorganisms and have the potential to be used as biological agents in controlling plant diseases. In this study, the VOCs produced by an acid-tolerant strain, Pseudomonas protegens CLP-6, showed strong antagonistic activities against phytopathogens, especially Ralstonia solanacearum, under acidic conditions. These VOCs could be used as an environmentally-friendly microbial fumigant against bacterial wilt of tobacco and other Solanaceae crops in acidic soils.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Plant Sciences
Lanping Shi, Xia Li, Yahong Weng, Hanyang Cai, Kaisheng Liu, Baixue Xie, Hussain Ansar, Deyi Guan, Shuilin He, Zhiqin Liu
Summary: The study reveals that plant immunity and dehydration tolerance are coupled and regulated by the CaPti1-CaERF3 module in pepper plants, enhancing resistance against bacterial wilt caused by Ralstonia solanacearum.
Article
Plant Sciences
Pau Sebastia, Roger de Pedro-Jove, Benoit Daubech, Anurag Kashyap, Nuria S. Coll, Marc Valls
Summary: This study systematically analysed the infection of R. solanacearum in S. dulcamara, showing that bittersweet plants exhibit partial resistance to bacterial wilt compared with susceptible hosts like tomatoes, especially at lower temperatures. The findings suggest that inherent resistance and perennial growth habits enable bittersweet plants to act as reservoirs for the pathogen.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Yifan Cao, Linda S. Thomashow, Yu Luo, Hangwei Hu, Xuhui Deng, Hongjun Liu, Zongzhuan Shen, Rong Li, Qirong Shen
Summary: Soil organic carbon, total nitrogen, available potassium, available phosphorus significantly influence the suppression of bacterial wilt disease, while the stoichiometry ratios such as carbon to nitrogen, nitrogen to phosphorus are also correlated with disease incidence and severity.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Plant Sciences
Bingsen Wang, Mengshu Huang, Wenfeng He, Yuqi Wang, Liu Yu, Dan Zhou, Chengzhen Meng, Dong Cheng, Huishan Qiu, Xiaodan Tan, Botao Song, Huilan Chen
Summary: Knock down of StTOPP6 enhances resistance to bacterial wilt in potato by activating the MAPK3-mediated pathway and inducing accumulation of reactive oxygen species.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Microbiology
Leo Gerlin, Antoine Escourrou, Cedric Cassan, Felicia Maviane Macia, Nemo Peeters, Stephane Genin, Caroline Baroukh
Summary: The plant pathogen Ralstonia solanacearum proliferates rapidly in xylem vessels, leading to plant death at a tipping point in bacterial wilt dynamics. Metabolic and physiological changes during infection are similar to drought stress. Glutamine is identified as the preferred substrate for R. solanacearum during colonization.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agronomy
Ippei Habe, Koji Miyatake
Summary: This study identified multiple major and minor resistance-related loci on potato chromosomes against different strains of Ralstonia solanacearum. The major QTLs PBWR-3 and PBWR-7 conferred stable broad-spectrum resistance, while PBWR-6b was a strain-specific major resistance QTL. These findings provide important theoretical basis for developing BW-resistant potato cultivars in specific regions.
MOLECULAR BREEDING
(2022)
Article
Virology
Abdelmonim Ali Ahmad, Kamel M. Elhalag, Hardian Susilo Addy, Mohamed A. Nasr-Eldin, Ahmed S. Hussien, Qi Huang
ARCHIVES OF VIROLOGY
(2018)
Article
Agronomy
Nurmalasari Darsono, Novita Niswatun Azizah, Kiky Mey Putranty, Natalia Tri Astuti, Hardian Susilo Addy, Win Darmanto, Bambang Sugiharto
Article
Virology
Hardian Susilo Addy, Moh Miftah Farid, Abdelmonim Ali Ahmad, Qi Huang
ARCHIVES OF VIROLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Alaaeldin Mohamed Saad, Ahmed Mahrous Soliman, Takeru Kawasaki, Makoto Fujie, Hirofumi Nariya, Tadashi Shimamoto, Takashi Yamada
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Microbiology
Hardian Susilo Addy, Abdelmonim Ali Ahmad, Qi Huang
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Emmanuelle Neumann, Takeru Kawasaki, Gregory Effantin, Leandro F. Estrozi, Orawan Chatchawankanphanich, Takashi Yamada, Guy Schoehn
JOURNAL OF GENERAL VIROLOGY
(2020)
Article
Virology
Takeru Kawasaki, Hisashi Endo, Hiroyuki Ogata, Orawan Chatchawankanphanich, Takashi Yamada
Summary: A novel lytic bacteriophage RP13 was isolated from tomato fields in Thailand, with unique features and heavily modified DNA. The whole genome of RP13 was determined to be 170,942 bp in length with 277 ORFs. Phylogenetic analysis revealed that RP13 is not closely related to any known phages, making it a potential biocontrol agent against bacterial wilt disease caused by R. solanacearum.
ARCHIVES OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ahmed Askora, Takeru Kawasaki, Makoto Fujie, Takashi Yamada
Summary: The study describes the integrative/excisive recombination reactions mediated by phi RSM integrase, showing efficient integration and excision in vitro without the need for additional proteins or high-energy cofactors. This small serine recombinase demonstrated two-way activity in excising a functional phage genome from a prophage fragment, marking a significant advancement in genome engineering applications.
MOLECULAR GENETICS AND GENOMICS
(2021)
Article
Biochemistry & Molecular Biology
Gregory Effantin, Akiko Fujiwara, Takeru Kawasaki, Takashi Yamada, Guy Schoehn
Summary: The phi RSA1 bacteriophage isolated from Ralstonia solanacearum exhibits unique structural characteristics, predominantly composed of a polymer of the gp8 protein, unlike other bacteriophages in terms of stabilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Abdelmonim Ali Ahmad, Hardian Susilo Addy, Qi Huang
Summary: A jumbo phage RsoM2USA isolated from a tomato field in Florida, U.S., has a long latent period and short infection cycle. With a genome size of 343,806 bp, it is the largest Ralstonia-infecting phage sequenced to date, belonging to the family Myoviridae. It infects multiple Ralstonia species and significantly reduces the virulence of susceptible strains in tomato plants.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
D. C. Widianingrum, H. Khasanah, H. S. Addy
Summary: This study investigated the presence of antibiotic-resistant genes in cases of Staphylococcal subclinical-mastitis in several regions of East Java Province, Indonesia. The results showed that antibiotic-resistant genes were found in non-aureus Staphylococci species in several areas, providing important information for the treatment of Staphylococcal subclinical-mastitis.
TROPICAL ANIMAL SCIENCE JOURNAL
(2022)
Article
Entomology
Sigit Prastowo, Soeharto, Hardian Susilo Addy, Tri Handoyo
Summary: Thirteen isolates of Metarhizium species were identified, with M. majus showing high pathogenicity against O. rhinoceros larvae. The close resemblance between M. majus isolates and CRB-isolated strains suggests a possible relationship between pathogenicity and host specificity with phylogenetic status or DNA sequence polymorphisms.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2022)
Meeting Abstract
Plant Sciences
A. A. Ahmad, H. S. Addy, Q. Huang
Proceedings Paper
Horticulture
A. Bhunchoth, N. Phironrit, C. Leksomboon, T. Kawasaki, T. Yamada, O. Chatchawankanphanich
V INTERNATIONAL SYMPOSIUM ON TOMATO DISEASES: PERSPECTIVES AND FUTURE DIRECTIONS IN TOMATO PROTECTION
(2018)
