Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Review
Biotechnology & Applied Microbiology
M. Mustafa Azeem, Muhammad Shafa, Muhammad Aamir, Muhammad Zubair, Basma Souayeh, Mir Waqas Alam
Summary: The recent pandemic has led to the development of new nucleic acid sensors with high detection accuracy. Various techniques using low-dimensional materials have been demonstrated to detect infinitesimal limits effectively. The research in this field aims to enhance the detection limit of sensors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Linlin Zhuang, Jianbo Yang, Chunlei Song, Li Sun, Bin Zhao, Qiuping Shen, Xiyan Ren, Hongjing Shi, Yu Zhang, Mengling Zhu
Summary: An accurate and practical graphene oxide-based method, called GO-ASEA, was developed for rapid and highly sensitive detection of African swine fever virus (ASFV). This novel assay showed excellent specificity and selectivity, with a low detection limit, and demonstrated good clinical applicability. The GO-ASEA method has the potential to serve as a robust tool in epidemic prevention and point-of-care diagnosis.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Bobin Lee, Yeonghun Lee, Seung-Mo Kim, Kihyeun Kim, Min-Gon Kim
Summary: The incidence of deadly infectious diseases has been increasing worldwide in recent years, leading to a high demand for rapid and accurate nucleic acid diagnostic systems. In this study, a rapid membrane-based photothermal polymerase chain reaction (PCR) system was developed, allowing for quantitative fluorescence detection. This system overcomes the limitations of conventional PCR and offers user-friendly micro-scale solution control and sample loading. The reaction pad is coated with an Au nanostructure that acts as a light-to-heat converter for thermocycling. The reaction solution is heated using a 785 nm light source, and the temperature is cycled 25 times between 63°C and 95°C within 6 minutes. The amplified samples on the membrane are then quantitatively analyzed by measuring the fluorescence signal. This membrane-based PCR approach can be easily integrated with various sample pre-treatment processes, making it a promising all-in-one molecular diagnosis platform.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Man Ho, N. Sathishkumar, Alexandros A. Sklavounos, Jianxian Sun, Ivy Yang, Kevin P. Nichols, Aaron R. Wheeler
Summary: This study developed a new microfluidic system that combines digital microfluidics (DMF) with distance-based detection (DBD) for LAMP-based point-of-care nucleic acid amplification tests (POC NAATs). The system allows for direct signal readout without the need for dedicated optical detection apparatus. The method was validated for SARS-CoV-2 detection in saliva and showed promising results. The combination of DMF with DBD holds great potential for implementing various POC NAATs or other applications in the future.
Article
Microbiology
Moussa El Jarroudi, Fadia Chairi, Louis Kouadio, Kathleen Antoons, Abdoul-Hamid Mohamed Sallah, Xavier Fettweis
Summary: The study proposed a weather-based modeling approach to predict infection by Cercospora beticola in sugar beet fields in Belgium, evaluating 14 models with M1, M2, and M3 performing outstandingly. These models can help predict CLS infection events and aid in determining the need for fungicide spray application on sugar beets.
Article
Chemistry, Analytical
Elena Suprun, Svetlana A. Khmeleva, Konstantin Bibik, Konstantin G. Ptitsyn, Leonid K. Kurbatov, Sergey P. Radko
Summary: In this study, modified dUTP-Fl and dUTP-Rh were used as substrates and electroactive labels for electrochemical detection of dsDNA amplification products. The results showed that dUTP-Fl had better compatibility with PCR and RPA, allowing the direct electrochemical detection of full-size amplicons at micromolar concentrations when substituted in the reaction mixture at 50-90% of dTTP.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Dalton J. Nelson, Mindy Leelawong, Megan E. Pask, C. William Wester, Muktar H. Aliyu, Frederick R. Haselton
Summary: This report presents the first step towards a highly specific SNP detection method using magnetic bead processing and PCR detection, which is suitable for resource-limited clinics and allows for more effective clinical management of drug resistance mutations.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Daya Marasini, Melissa J. Whaley, Laurel T. Jenkins, Fang Hu, Wenxin Jiang, Nadav Topaz, Alex Chen, Susanna Schmink, Jennifer Dolan Thomas, Brian H. Harcourt, Henju Marjuki, Xin Wang
Summary: We have developed direct real-time PCR assays for the detection of Haemophilus influenzae (Hi) and its six serotypes directly from cerebrospinal fluid specimens. These assays are highly sensitive and specific, do not require DNA extraction, and can detect three serotypes in a single reaction, improving testing efficiency.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Review
Oncology
Jordana Williams, Morris Kostiuk, Vincent L. Biron
Summary: HPV is responsible for most cervical cancers and some head and neck cancers. Specific detection methods are used to diagnose HPV infections in cervical and head and neck cancers.
FRONTIERS IN ONCOLOGY
(2022)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Veterinary Sciences
Zoran Zlabravec, Brigita Slavec, Al Vrezec, Urska Kuhar, Olga Zorman Rojs, Zlatko Golob, Jozko Racnik
Summary: This study detected the presence of herpesviruses in wild birds and investigated their phylogenetic relationship using PCR. The results showed that herpesviruses are present in a wide range of bird species and can be transmitted through predation and superpredation. Simultaneous testing of oropharyngeal and cloacal swabs is necessary for accurate herpesvirus detection in wild birds.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Haoyi Xu, Hangzhen Lan, Daodong Pan, Junfeng Xu, Xiaofu Wang
Summary: The aim of this study was to develop an accurate, easy-to-use, and cost-effective method for detecting chicken adulteration using PCR and LFS. The study compared six DNA extraction methods and identified a suitable one. The PCR-LFS method demonstrated high specificity and sensitivity, providing accurate results in a short time.