Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 120, Issue 2, Pages 563-570Publisher
SPRINGER
DOI: 10.1007/s11240-014-0622-1
Keywords
Grasswort; Halophyte; Magnesium chloride; Regeneration; Root culture; Salicornia brachiata
Funding
- CSIR, New Delhi, India [OLP0058]
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An improved micropropagation protocol has been developed for halophytic grasswort Salicornia brachiata by using double concentration of Murashige and Skoog (Physiol Plant 15:473-497, 1962) medium (DMS) supplemented with plant growth regulators. Best shoot bud induction (90 %) from nodal explants was observed on DMS supplemented with 3.0 mg L-1 6-benzyl aminopurine (BAP) and 0.5 mg L-1 Zeatin with > 3 shoot buds. Shoot proliferation and elongation was achieved on DMS supplemented with 1.0 mg L-1 thidiazuron (TDZ) and 1.0 mg L-1 alpha-naphthalene acetic acid (NAA) with multiple shoot buds after 30 days of culture. Best callus response (78 %) was observed when stem explants were cultured on DMS supplemented with 2.0 mg L-1 2, 4-dichlorophenoxyacetic acid and 0.01 mg L-1 BAP. Regeneration from the callus was achieved when callus was cultured on DMS supplemented with 0.5 mg L-1 TDZ. These shoot buds were elongated on shoot proliferation and elongation medium. Elongated shoots (5 cm) could be rooted on DMS supplemented with 0.5-1.5 mg L-1 NAA, 0.5-1.5 mg L-1 indole-3-acetic acid and 0.5-1.5 mg L-1 indole-3-butyric acid. DMS medium supplemented with 0.5 mg L-1 NAA found to be best for rooting and the addition of 20 g L-1 magnesium chloride (MgCl2) to the medium resulted in highest percentage of rooting and not with the addition of sodium chloride (NaCl). Rooted plants could be established in soil with 55 % survival. In another set of study on root culture generation, many roots initiated from explants cultured on DMS supplemented with 2.0 mg L-1 NAA. Addition of 10 g L-1 MgCl2 was highly beneficial in stimulating root initiation and proliferation as compared to NaCl.
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