Article
Agronomy
Raoni Andrade Pires, Onildo Nunes Jesus, Lucas Kennedy Silva Lima, Luana Nascimento Silva, Francisco Ferraz Laranjeira
Summary: Genetic variability among Fusarium oxysporum f. sp. passiflorae (FOP) isolates affects their aggressiveness, with isolate CMF3115 being the most aggressive in this study. This isolate also showed high levels of chlamydospores in roots, suggesting potential for screening Passiflora germplasm for resistance to FOP.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Plant Sciences
R. Thangavel, J. A. Pattemore, K. B. Rebijith, N. Grbavae, S. Ganev, N. Chan, H. G. Pearson, B. J. R. Alexander
Summary: The study confirmed the presence of Fusarium oxysporum f. sp. passiflorae (FOP) in Northland, New Zealand, through pathogenicity testing and DNA sequencing. The FOP isolates in New Zealand were found to have identical SIX6 and SIX9 sequences, indicating a shared homolog of these effector genes among different haplotypes.
AUSTRALASIAN PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Cui Yang, Jing Sun, Zhaoqun Wu, Maozhu Jiang, Dayong Li, Xinjie Wang, Chunxiang Zhou, Xuecheng Liu, Zhiguo Ren, Jun Wang, Manli Sun, Wenxian Sun, Jie Gao
Summary: In this study, the FoRSR1 gene encoding a Ras-related small GTPase in F. oxysporum f. sp. ginseng was identified and characterized. Disruption of FoRSR1 affected mycelial dry weight, hyphal morphology, conidiation, chlamydospore formation, and virulence in ginseng roots. Deletion of FoRSR1 also had impacts on pigmentation, autophagy, and secondary metabolism. The study demonstrated the important roles of FoRSR1 in various biological processes and pathogenesis in F. oxysporum f. sp. ginseng.
Review
Biochemistry & Molecular Biology
Owen Hudson, James C. Fulton, Alexi K. Dong, Nicholas S. Dufault, Md Emran Ali
Summary: Watermelon is an important commercial crop, but its production is significantly limited by fusarium wilt caused by the fungus Fon. Management strategies include rotation, improved resistance, and chemical control, but accurate diagnostics are crucial. Molecular strategies for diagnostics greatly reduce the time needed for diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anastasia Samsonova, Alexander Kanapin, Michael Bankin, Anton Logachev, Maria Gretsova, Tatyana Rozhmina, Maria Samsonova
Summary: The study revealed the complex genomic architecture of Fusarium oxysporum f. sp. lini, shedding light on its diverse evolutionary dynamics that enhance genetic diversity and facilitate host adaptation. Experimental results indicate that mobile genetic elements are expressed in planta upon infection, suggesting a potential role in pathogenicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Qinggang Guo, Shixin Li, Lihong Dong, Zhenhe Su, Peipei Wang, Xiaomeng Liu, Ping Ma
Summary: This study established a screening method for biocontrol agents against Fusarium wilt based on tolerance to Fusaric acid and antagonism against F. oxysporum. Three promising biocontrol bacteria were obtained, named B31, F68, and 30833, which successfully controlled tomato, watermelon, and cucumber Fusarium wilt. Phylogenetic analysis confirmed these strains as B. velezensis. Further experiments showed that strains B31, F68, and 30833 exhibited significantly greater tolerance to Fusaric acid compared to strain FZB42.
Article
Microbiology
Yan-Fen Wang, Fang-Min Hao, Huan-Huan Zhou, Jiang-Bo Chen, Hai-Chuan Su, Fang Yang, Yuan-Yuan Cai, Guan-Long Li, Meng Zhang, Feng Zhou
Summary: Melon Fusarium wilt (MFW), caused by Fusarium oxysporum f. sp. melonis (FOM), is a soil-borne disease that commonly affects melon cultivation worldwide. Fludioxonil, a phenylpyrrole fungicide, has strong inhibitory effects on the mycelial growth of FOM isolates. Mutant FOM isolates with fludioxonil resistance showed reduced sporulation and growth rates in response to certain stimuli. Molecular analysis suggested that the observed fludioxonil resistance was related to changes in the sequence and expression of the FoOs1 gene. No cross-resistance was found between fludioxonil and other tested fungicides. Fludioxonil has great potential as an alternative control method for FOM in melon crops.
Article
Plant Sciences
Yi Zhu, Abdelraheem Abdelraheem, Phillip Lujan, John Idowu, Patrick Sullivan, Robert Nichols, Tom Wedegaertner, Jinfa Zhang
Summary: The study conducted a field survey in three southern counties of New Mexico and identified FOV4, a threat to cotton production, with different levels of virulence. Different cotton cultivars showed varying degrees of resistance to FOV4.
Article
Biochemistry & Molecular Biology
Ping Xingxing, Raja Asad Ali Khan, Li Yan, Yang Yuhong, Xie Bingyan, Mao Zhenchuan, Ling Jian
Summary: Fusarium oxysporum f. sp. capsici is the specific pathogen of pepper Fusarium wilt and its genome assembly serves as a valuable resource for research.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Horticulture
Juliana Avelar de Carvalho, Jeferson Goncalves de Jesus, Kelly Lana Araujo, Milson Evaldo Serafim, Thiago Alexandre Santana Gilio, Leonarda Grillo Neves
Summary: The study evaluated the resistance/susceptibility of Passiflora species to soil pathogens in different biomes of Mato Grosso, with P.nitida, foetida, and P.mucronata showing the highest resistance levels. This suggests that these genotypes can be utilized as a source of resistance in commercial passionfruit breeding programs.
REVISTA BRASILEIRA DE FRUTICULTURA
(2021)
Review
Plant Sciences
Lynn Epstein, Sukhwinder Kaur, Peter M. Henry
Summary: Foa race 4 and Foci strains have emerged as infectious plant diseases in celery and coriander in coastal southern California. They are spreading rapidly and causing severe yield losses, with no economical solutions for control currently available. Foa race 4 has been observed to cause more severe disease at temperatures above 21 degrees Celsius.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Fangwei Yu, Wei Zhang, Shenyun Wang, Hong Wang, Li Yu, Xiaoping Zeng, Zhangjun Fei, Jianbin Li
Summary: This study presents a high-quality genome sequence of a race 1 strain of F. oxysporum f. sp. conglutinans, providing valuable insights into its interaction with cabbage.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Biochemical Research Methods
Eman Elagamey, Magdi A. E. Abdellatef, Md Yasir Arafat
Summary: This study provides insights into the inhibitory mechanism of chitosan against Fusarium oxysporum f. sp. cucumerinum (FOC) by identifying chitosan-responsive proteins (CRPs). It is found that chitosan can restrict disease severity, affect the metabolism and defense of FOC, and inhibit the synthesis of proteins responsible for FOC's virulence.
JOURNAL OF PROTEOMICS
(2022)
Article
Plant Sciences
Graziele Santos Lima, Leandro de Souza Rocha, Hermes Peixoto Santos-Filho, Sami Jorge Michereff, Francisco Ferraz Laranjeira
Summary: This study simulated the in vitro dynamics of the plant pathogenic fungus FOP under different environmental conditions and found that the nutrient state of the environment influenced the rate of pathogen spread, potentially slowing down epidemic progress. The research provides new biological knowledge and insights for disease management improvement.
TROPICAL PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuta Asai, Yu Ayukawa, Pamela Gan, Ken Shirasu
Summary: This study presents high-quality genome sequences of two pathogenic forms of Fusarium oxysporum isolated from radish and turnip, which can be utilized for investigating interactions with Brassicaceae plants and understanding the evolution of pathogenicity within the F. oxysporum species complex.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Biotechnology & Applied Microbiology
Daniele Vidal Faria, Debora Marcia Silva Freitas, Marcos Bruno Silva Duarte, Kleiton Lima Godoy Machado, Elyabe Monteiro Matos, Diego Silva Batista, Marcio Gilberto Cardoso Costa, Lyderson Facio Viccini, Fabio Tebaldi Silveira Nogueira, Wagner Campos Otoni
Summary: An improved method for the Agrobacterium-mediated transformation of hypocotyl segments of annatto was developed and successfully validated by producing transgenic plants overexpressing microRNA 156. The optimized protocol achieved the highest transformation frequency reported for the target species.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Forestry
Tamyris de Mello, Thuanny Lins Monteiro Rosa, Ingridh Medeiros Simoes, Paula Aparecida Muniz de Lima, Breno Benvindo dos Anjos, Caroline Palacio de Araujo, Clovis Eduardo Nunes Hegedus, Heloisa Oliveira dos Santos, Wagner Campos Otoni, Rodrigo Sobreira Alexandre, Jose Calos Lopes
Summary: The study revealed that Euterpe edulis seeds have sufficient reserves to develop normally after 148 days post-anthesis, resulting in a 100% germination rate and the formation of 100% normal seedlings. The morphological characteristics, germination, vigor, and biochemical properties of in vitro germinated seeds varied across different maturation stages, reflecting changes in the composition of the seeds as they mature.
TREES-STRUCTURE AND FUNCTION
(2022)
Editorial Material
Biotechnology & Applied Microbiology
S. Ochatt, A. R. Alan, A. Bhattacharya, C. Hano, K. Kiselev, P. L. Marconi, W. C. Otoni, S. Y. Park, K. X. Tang, P. J. Weathers
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Juliene D. R. Moreira, Bruno L. Rosa, Bruno S. Lira, Joni E. Lima, Ludmila N. F. Correia, Wagner C. Otoni, Antonio Figueira, Luciano Freschi, Tetsu Sakamoto, Lazaro E. P. Peres, Magdalena Rossi, Agustin Zsogon
Summary: The distribution of heterobaric and homobaric leaves in tomatoes is controlled by a single-nucleotide polymorphism in a transcription factor gene. This mutation exists in wild tomatoes and has increased in frequency in domesticated tomatoes, affecting leaf growth and morphological characteristics.
Article
Plant Sciences
Evandro Alexandre Fortini, Diego Silva Batista, Sergio Heitor Sousa Felipe, Tatiane Dulcineia Silva, Ludmila Nayara Freitas Correia, Leticia Monteiro Farias, Daniele Vidal Faria, Vitor Batista Pinto, Claudete Santa-Catarina, Vanildo Silveira, Clelia De-la-Pena, Eduardo Castillo-Castro, Wagner Campos Otoni
Summary: This study investigated the proteomic and epigenetic changes involved in the morphophysiological responses of Pfaffia glomerata to salt stress and the demethylating agent 5-azacytidine. The results revealed differentially accumulated proteins related to amino acid and carbohydrate metabolism, and specialized metabolism, as well as global DNA methylation levels and 20-E biosynthesis. The findings suggest the potential regulatory proteins and epigenetic changes that contribute to salt stress tolerance and 20-E biosynthesis in P. glomerata.
Article
Horticulture
Aressa de Oliveira Correia, Rodrigo Sobreira Alexandre, Ludwig Heinrich Pfenning, Patricia Alvarez Cabanez, Adesio Ferreira, Marcia Flores da Silva Ferreira, Paula Aparecida Muniz de Lima, Tamyris de Mello, Wagner Campos Otoni, Jose Carlos Lopes
Summary: This study assessed the resistance of ten P. mucronata genotypes to fusariosis. It found that G2 and G5 genotypes were immune to fusariosis, while G9 genotype showed horizontal resistance. These genotypes can be used as rootstocks for breeding.
SCIENTIA HORTICULTURAE
(2022)
Article
Multidisciplinary Sciences
Cristina P. S. Martins, Denise Fernandes, Valeria M. Guimaraes, Dongliang Du, Delmira C. Silva, Alex-Alan F. Almeida, Frederick G. Gmitter, Wagner C. Otoni, Marcio G. C. Costa
Summary: This study assessed the potential functions of citrus galactinol synthase (GolS) in stress response and tolerance mechanisms. The results showed that citrus GolS genes have low-level tissue-specific and stress-inducible expression in response to drought, salt stress, and infection. Overexpression of CsGolS6 improved tobacco tolerance to drought and salt stresses, leading to increased mesophyll cell expansion, photosynthesis, and plant growth. Metabolite profiling revealed no significant changes in endogenous galactinol, but an increase in metabolites with antioxidant properties was observed in the transgenic plants.
Article
Plant Sciences
Rodrigo Sobreira Alexandre, Caroline Palacio de Araujo, Andre Lucas Siqueira, Elisa Mitsuko Aoyama, Tamyris de Mello, Thuanny Lins Monteiro Rosa, Carlos Eduardo Valerio Raymundo, Edilson Romais Schmildt, Jose Carlos Lopes, Joao Paulo Bestete de Oliveira, Robson Bonomo, Wagner Campos Otoni
Summary: This study evaluated the influence of tendrils and indole-3-butyric acid (IBA) on the anatomy and adventitious rooting of cuttings from three black pepper cultivars. Results showed that IBA-treated cuttings without tendrils had the highest rooting rates. Histological and histochemical analyses revealed some anatomical characteristics and starch content in the cuttings were related to rooting rates.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Forestry
Natane Amaral Miranda, Aloisio Xavier, Wagner Campos Otoni
Summary: The mini-cutting method is widely used for Eucalyptus plantlet production, but it has issues with physiological vigor loss and low rooting rates. In vitro culture techniques offer advantages, but they still cause some stress to plants and have limitations for commercial use. This study proposes an in vitro clonal hedge system, combining traditional techniques, to overcome the difficulties in mini-clonal hedge propagation. High survival and rooting rates were observed in both systems. However, further research and improvement are needed for the in vitro clonal hedge system to become an alternative for clonal Eucalyptus plantlet production.
Article
Ecology
Allan Rocha de Freitas, Nathalia Aparecida Braganca Favaris, Rodrigo Sobreira Alexandre, Tatiana da Silva Souza, Iasmini Nicoli Galter, Julcinara Oliveira Baptista, Paula Aparecida Muniz de Lima, Tamyris de Mello, Wagner Campos Otoni, Jose Carlos Lopes
Summary: The aim of this study was to investigate the effect of sewage sludge (SS) dosage on the cell cycle and growth of Lactuca sativa and Passiflora alata. The results showed that SS concentrations above 120 t ha(-1) adversely affected the germination and early growth of L. sativa and P. alata, with high concentrations inducing genetic damage and chromosomal and nuclear changes in L. sativa.
Article
Biotechnology & Applied Microbiology
Tamyris de Mello, Ludmila Nayara Freitas Correia, Clovis Eduardo Nunes Hegedus, Edilson Romais Schmildt, Adesio Ferreira, Jose Carlos Lopes, Wagner Campos Otoni, Rodrigo Sobreira Alexandre
Summary: Somatic embryogenesis of E. edulis stem explants is not affected by their polarity, and a concentration of 150 mu M PIC in the culture medium results in the highest number of embryos. After 30 days of maturation, the explants should be transferred to a different medium to avoid oxidation. Around 20% of somatic embryos successfully convert into plantlets and can be acclimatized.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Viviane Santos Moreira, Vinicius Carius de Souza, Virginia Lucia Fontes Soares, Aurizangela Oliveira Sousa, Katiucia Ticila de Souza de Nascimento, Monique Reis de Santana, Tiyoko Nair Hojo Reboucas, Carlos Andre Espolador Leitao, Priscila Vanessa Zabala Capriles Goliatt, Daniele Vidal Faria, Wagner Campos Otoni, Marcio Gilberto Cardoso Costa
Summary: By combining chemical, anatomical, and transcriptomic data, this study provides insights into the dynamics and regulation of Bixin biosynthesis and accumulation during B. orellana seed development. Bixin accumulated in large quantities in seeds, reached a peak at the S4 stage, and gradually decreased thereafter. Anatomical analysis revealed that bixin accumulated in the central vacuole of specialized cells in the developing mesotesta. Transcriptomic analysis identified 312 genes involved in the DOXP/MEP, carotenoid, and bixin biosynthesis pathways, with 50 of them being sequentially and differentially expressed throughout seed development.
Article
Biotechnology & Applied Microbiology
Lais Stehling de Queiroz Nascimento, Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Vinicius Carius Souza, Diego Silva Batista, Marcelo de Oliveira Santos, Wagner Campos Otoni, Lyderson Facio Viccini
Summary: Experimental conditions affect the expression of reference genes. NADH is the most stable gene under saline, exogenous hormones, wounding, and cold stress; whereas TUB is most stable under osmotic stress. Lippia alba is a medicinal plant with commercial value as a source of essential oils. RT-qPCR is a useful tool for studying gene expression and selecting improved essential oil composition. TUB and NADH are recommended as reference genes for expression studies in L. alba.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Agriculture, Multidisciplinary
Ingridh Medeiros Simoes, Caroline Palacio de Araujo, Tamyris de Mello, Thuanny Lins Monteiro Rosa, Natasha Vieira de Oliveira, Marcos Vinicius Winckler Caldeira, Edilson Romais Schmildt, Jose Carlos Lopes, Wagner Campos Otoni, Rodrigo Sobreira Alexandre
Summary: The objective of this study was to establish an efficient protocol for in vitro organogenesis of Dalbergia nigra. It was found that explants from 30-day-old cotyledonary nodes showed a higher morphogenetic potential, and the medium supplemented with 2.22 μmol/L BAP was the best combination for in vitro propagation of D. nigra.
PESQUISA AGROPECUARIA BRASILEIRA
(2022)
Review
Biochemistry & Molecular Biology
Joao Antonio Siqueira, Wagner C. Otoni, Wagner L. Araujo
Summary: Efficient use of natural resources can be improved through tailored developmental programs that maximize plant lifetime and fitness. Root development consists of three main phases: root apical meristem appearance, foraging, and senescence. These phases are regulated by four developmental pathways: autonomous, non-autonomous, hormonal, and periodic, which coordinate organ plasticity and nutritional checkpoints.
PLANT COMMUNICATIONS
(2022)