Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 101, Issue 1, Pages 59-63Publisher
SPRINGER
DOI: 10.1007/s11240-009-9664-1
Keywords
Alliin; Embryos; Garlic; In vitro; Sulphur
Funding
- Dean, Faculty of Science
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Garlic extract contains alliin (S-allyl-l-cysteine sulfoxide), an organosulphur compound that contributes to its therapeutic value and pharmacological importance. Alliin production in plant organs (leaf and root), plantlet, callus (non-embryogenic and embryogenic), embryo (proliferated, matured and germinated embryos), grown under in-vitro conditions was evaluated after 8 weeks. Highest alliin content was recorded in leaves. Clove callus and germinated embryos also showed alliin production. Evaluation of alliin content of in-vitro grown tissues both in normal (control) and sulphur supplemented conditions (4, 8, 16, 32 mg l(-1)) showed that sulphur treatment at supply of 16 mg l(-1) gypsum (CaSO4) significantly enhanced the production of alliin content in all in-vitro grown tissues and organs. These findings suggest that production of alliin is dependent upon type and age of tissue and can be altered by growth conditions.
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