Article
Plant Sciences
Dipti Tonk, Abdul Mujib, Mehpara Maqsood, Mir Khusrau, Ali Alsughayyir, Yaser Hassan Dewir
Summary: This study investigated the effect of Fusarium oxysporum on the yields of vinblastine and vincristine alkaloids in Catharanthus roseus embryos. The addition of F. oxysporum extract improved the growth of callus biomass and enhanced the accumulation of vinblastine and vincristine in the tissues. Low doses of the Fusarium treatment resulted in high germination rates and longer shoot and root lengths of somatic embryos. The yields of vinblastine and vincristine were measured, with the highest and increased yields observed in F. oxysporum-amended tissues.
Article
Plant Sciences
Zahid Hameed Siddiqui, Abdul Mujib, Zahid Khorshid Abbas, M. Salik Noorani, Salim Khan
Summary: The use of CaCl2 as an elicitor was found to enhance Vinblastine synthesis in Catharanthus roseus cell suspension culture, leading to increased productivity and reduced production costs.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Plant Sciences
Tran My Linh, Nguyen Chi Mai, Pham Thi Hoe, Ninh Thi Ngoc, Phan Thi Hong Thao, Ninh Khac Ban, Nguyen Tuong Van
Summary: Cell and tissue cultures of Catharanthus roseus have been studied extensively to improve the production of valuable secondary metabolites. The study successfully produced callus and suspension cell biomass with high quality and quantity, while utilizing endophytic fungi to enhance alkaloid accumulation.
Article
Biotechnology & Applied Microbiology
A. Mujib, Samar Fatima, Moien Qadir Malik
Summary: The study investigated the impact of gamma ray irradiation on callus biomass growth and the yield of vincristine and vinblastine in in vitro grown tissues of Catharanthus roseus. The lower gamma irradiation levels of 20-40 Gy were found to be more effective in enriching alkaloids, while higher doses declined the yield.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Plant Sciences
Anamika Paul, Krishnendu Acharya, Nilanjan Chakraborty
Summary: The demand for natural products has led to increased interest in the medicinal plant Catharanthus roseus. This plant is known for its traditional pharmacological applications and its rich phytochemical composition, particularly alkaloids. Among these alkaloids, vinblastine and vincristine are of great importance as expensive anti-cancer compounds. This review provides an overview of the biosynthesis, extraction, detection, mode of action, and limitations of vinblastine and vincristine, aiming to contribute to the understanding of their mechanism of action and potential side effects. The need for increasing the production of these alkaloids using cost-effective biotechnological methods is also highlighted.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Agricultural Engineering
Xinyu Yang, Ru Zhao, Han Wang, Ailing Ben, Hong Lin, Xiaonan Zhang, Chunying Li, Lei Yang
Summary: This study developed a method for simultaneous enrichment and separation of vindoline, catharanthine, and vinblastine from Catharanthus roseus ionic liquid extracts. HPD750 resin was found to have higher adsorption capacity and desorption ratio for the target alkaloids compared to other resins. After optimization, the content and recovery of the target alkaloids in the products met satisfactory levels.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Genetics & Heredity
Pengfei Zhou, Mingxiang Chen
Summary: This research compared the alkaloid concentrations and transcriptomes of cambial meristematic cells (CMCs) and dedifferentiated cells (DDCs) in Catharanthus roseus. The results showed that CMCs produced significantly higher concentrations of alkaloids compared to DDCs. Transcriptomic analysis revealed differential expression of genes involved in alkaloid biosynthesis and hormone metabolism, providing potential target genes and regulators for developing an efficient alkaloid production system using CMCs.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Guoqing Song, Qiuxia Chen, Pete Callow, Mario Mandujano, Xiaoyan Han, Beatriz Cuenca, Gregory Bonito, Carmen Medina-Mora, Dennis Wayne Fulbright, Daniel E. Guyer
Summary: The efficient method described in the study involves micropropagation of European ? Japanese chestnut hybrids using specific media and plant growth hormones, resulting in successful shoot multiplication and rooting. Acclimatization of rooted shoots under high humidity conditions showed high success rates within a short period of time.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Agricultural Engineering
Elham Azadvari, Ziba Ghasimi Hagh, Amin Ebrahimi, Hojatollah Bodaghi
Summary: This study aimed to improve the production of secondary metabolites in Catharanthus roseus and investigate the effects of PEG-induced drought stress on physiological and biochemical characteristics. The results showed that increasing PEG concentration led to higher levels of malondialdehyde and proline in the Alba cultivar compared to the Rosea cultivar. The Alba cultivar also exhibited higher activity of antioxidant enzymes. The relative expression of vinblastine biosynthesis genes was significantly increased in both cultivars, especially in the medium with 5% PEG. Similarly, the relative expression of CrMPK3 and catalase genes increased significantly in all PEG-containing media. These findings suggest that PEG can induce the accumulation of vinblastine through a signaling pathway involving MAPK and antioxidant enzymes.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Kanchan Birat, Reem Binsuwaidan, Tariq Omar Siddiqi, Showkat Rasool Mir, Nawaf Alshammari, Mohd Adnan, Rahila Nazir, Bushra Ejaz, Moien Qadir Malik, Rikeshwer Prasad Dewangan, Syed Amir Ashraf, Bibhu Prasad Panda
Summary: A novel endophytic fungus, Nigrospora zimmermanii, was isolated from Catharanthus roseus and found to produce the anticancer compound vincristine. The fungus showed the ability to re-infect the plant after 20 days of inoculation, highlighting its potential as an alternative method for high yield production of vincristine compared to plant-mediated extraction.
Article
Chemistry, Applied
D. A. Zavala-Ortiz, M. J. Martinez-Montero, E. Guedon, A. Marc, B. Ebel, D. M. Barradas-Dermitz, P. M. Hayward-Jones, M. Mata-Rosas, M. G. Aguilar-Uscanga
Summary: Plant cell culture technology, especially suspension cultures, is a convenient tool for industrial production of molecules like vincristine and vinblastine. Cell differentiation is necessary for in vivo synthesis, with culture conditions such as light exposure and plant growth regulator regimes affecting differentiation and titers of these molecules. The study provides important insights for understanding in vitro culture performance and developing advanced processes for producing valuable molecules.
REVISTA MEXICANA DE INGENIERIA QUIMICA
(2021)
Article
Biotechnology & Applied Microbiology
Ahmed Madi Waheed Al-Mayahi
Summary: The addition of polyamines and silver thiosulfate to the nutrient medium improves shoot organogenesis, rooting, and production of genetically stable date palm plants.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
M. Ahmadzadeh, A. H. Keshtkar, K. Moslemkhany, M. Ahmadzadeh
Summary: This study investigated the effects of plant probiotic bacteria on the expression of TIA biosynthetic pathway genes and alkaloid content in Catharanthus roseus. The results showed that P. fluorescens and A. brasilense significantly increased the alkaloid content in roots, as well as the expression of genes in the biosynthetic pathway. The findings suggest that seed and seedling root treatments with naturally occurring probiotic bacteria could be effective in enhancing alkaloid contents in medicinal plants.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Plant Sciences
Joana G. Guedes, Rogerio Ribeiro, Ines Carqueijeiro, Ana Luisa Guimaraes, Claudia Bispo, John Archer, Herlander Azevedo, Nuno A. Fonseca, Mariana Sottomayor
Summary: The mesophyll idioblasts of Catharanthus roseus are a hotspot for alkaloid accumulation and have a distinctive transcriptomic profile associated with stress responses. The transcriptome of idioblasts may hold the key to understanding the MIA pathway and increasing the levels of anticancer drugs.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Shaghufta Perveen, Naila Safdar, Azra Yasmin, Yamin Bibi
Summary: The underlying mechanism of nanostructures induced by monoterpene in Catharanthus roseus has not been explored yet. In this study, Copper, Iron, and Zinc nanoparticles were synthesized using Eriobotrya japonica seed extract and capped with reduced glutathione. These nanoparticles were used in plant tissue culture media and were found to stimulate the production of alkaloids, tannins, and flavonoids in C. roseus without affecting its growth. Copper and Zinc nanostress resulted in the highest vincristine production in propagated calli and shoots cultures, while iron nanostructure had the least effect. Nano-zinc was also found to increase the expression of DAT and PRX1 genes in the TIA pathway. The accumulation of calcium in in vitro shoots was highest under capped and uncapped Zinc nanoparticles compared to control. This study highlights the potential of metal biogenic nanomaterials as drug modulators in plant tissue culture studies.
Article
Biochemical Research Methods
Shivani Saini, Navdeep Kaur, Pratap Kumar Pati
ANALYTICAL BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Neha Basotra, Swati Joshi, T. Satyanarayana, Pratap Kumar Pati, Adrian Tsang, Bhupinder S. Chadha
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Multidisciplinary Sciences
Amandeep Kaur, Baldev Singh, Puja Ohri, Jia Wang, Renu Wadhwa, Sunil C. Kau, Pratap Kumar Pati, Arvinder Kaurl
Article
Plant Sciences
Gurpreet Kaur, Pratap Kumar Pati
Article
Biotechnology & Applied Microbiology
Ashwani Kumar Bhardwaj, Baldev Singh, Kulwinder Kaur, Poonam Roshan, Anu Sharma, Dechen Dolker, Avilekh Naryal, Shweta Saxena, Pratap Kumar Pati, Om Prakash Chaurasia
PLANT CELL TISSUE AND ORGAN CULTURE
(2018)
Article
Multidisciplinary Sciences
Vasudha Gupta, Rupam Guleri, Muskan Gupta, Navdeep Kaur, Kuldeep Kaur, Paramdeep Kumar, Manju Anand, Gurcharan Kaur, Pratap Kumar Pati
Article
Multidisciplinary Sciences
Amritpreet Kaur, Pratap Kumar Pati, Aparna Maitra Pati, Avinash Kaur Nagpal
Article
Agricultural Engineering
Baldev Singh, Navdeep Kaur, Paramdeep Kumar, Vipin Hallan, Pratap Kumar Pati
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Plant Sciences
Shivani Saini, Navdeep Kaur, Deeksha Marothia, Baldev Singh, Varinder Singh, Pascal Gantet, Pratap Kumar Pati
Summary: The study revealed that the salt tolerant rice cultivar Luna Suvarna exhibited stress adaptive root traits and higher accumulation of auxin compared to the salt sensitive cultivar IR64. Luna Suvarna also showed increased levels of auxin homeostasis genes transcript under salinity stress, along with differential regulation of salinity stress responsive proteins in its roots, highlighting the morphological and molecular features that contribute to its salinity stress tolerance.
Article
Agricultural Engineering
Kuldeep Kaur, Kulwinder Kaur, Abhishek Bhandawat, Pratap Kumar Pati
Summary: This study successfully selected an elite accession of Withania somnifera with high withaferin A content and utilized meta-Topolin (mT) as a novel cytokinin for aseptic cultures, achieving clonal mass propagation of shoots. Additionally, an effective regeneration protocol using leaf explant of W. somnifera for in vitro mass propagation and genetic transformation studies was developed. The genetic uniformity of the in vitro regenerated shoots was confirmed using RAPD, ISSR, and SCoT markers.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Review
Biochemistry & Molecular Biology
Navdeep Kaur, Shubham Sharma, Mirza Hasanuzzaman, Pratap Kumar Pati
Summary: The susceptibility of crop plants towards abiotic stresses poses a significant threat to global food security. Traditional methods such as plant breeding and genetic engineering have limitations in enhancing stress resilience due to the complex inheritance of adaptive traits. Genome editing has emerged as a promising approach to address this challenge by improving stress tolerance in crops with high precision.
INTERNATIONAL JOURNAL OF GENOMICS
(2022)
Review
Plant Sciences
Isha Sharma, Pulugurtha Bharadwaja Kirti, Pratap Kumar Pati
Summary: Manipulation of the autophagic pathway offers great potential for designing climate-smart crops with improved yield and adaptability. Identifying and characterizing the adapter/receptor complex and understanding its regulatory network in crop plants is crucial for fully utilizing autophagy. Recent advances have revealed plant-specific roles of autophagy and its implications in improving stress tolerance, disease resistance, yield potential, and production of economically valuable metabolites. Bridging knowledge gaps and developing biotechnological applications of autophagy can enhance bioeconomy and sustainability in agriculture.
Review
Plant Sciences
Anshika Sethi, Abhishek Bhandawat, Pratap Kumar Pati
Summary: Cytochrome P450 enzymes play a critical role in the biosynthesis of secondary metabolites and show great potential as targets for metabolic pathway engineering. They are found in all life domains and have been successfully utilized in medicinal plants. Pathway engineering has achieved significant improvements in the production of important bioactive compounds, and strategies to overcome limitations associated with CYP engineering are being explored.
Article
Plant Sciences
P. Kumar, B. Singh, S. Rajak, S. Pandey, P. K. Pati
Summary: This study investigated the response of Withania somnifera to leaf spot disease caused by Alternaria alternata. It was found that the infected leaves showed increased NADPH oxidase activity and ROS accumulation, as well as enhanced antioxidant enzyme activity and lignin content. The expression of lignin biosynthesis genes and defence-related genes was also upregulated. This study provides insights into the defence mechanisms of W. somnifera against leaf spot disease.
Article
Biochemistry & Molecular Biology
Gurpreet Kaur, Kunchur Guruprasad, Brenda R. S. Temple, David G. Shirvanyants, Nikolay V. Dokholyan, Pratap Kumar Pati
