Article
Genetics & Heredity
Jia Yan, Ha Buer, Ya Ping Wang, Gegen Zhula, Yu'e Bai
Summary: Transcriptomic analysis of zygotic embryos in Picea mongolica revealed transcripts related to embryogenic competence, cell division pattern, hormones, and stress response genes. Auxin is crucial for early embryo patterning and pre-cotyledon embryonic formation, while ABA regulates embryo maturation. Methylation-related gene expression is associated with activation of early-stage embryos, late embryogenesis abundant proteins, and storage/energy-related genes in late and mature embryos. Network analysis identified stage-specific and multistage gene expression clusters during embryogenesis, with WOX, MYB, AP2, and HLH transcription factors playing a role in different stages of embryogenesis. This study provides valuable transcriptome data for embryo development in P. mongolica, benefiting the protection and utilization of its genetic resources.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Janet Juarez-Escobar, Esau Bojorquez-Velazquez, Jose M. Elizalde-Contreras, Jose A. Guerrero-Analco, Victor M. Loyola-Vargas, Martin Mata-Rosas, Eliel Ruiz-May
Summary: Embryogenesis is a crucial developmental program in plants, with research gaps in understanding the mechanisms regulating somatic embryogenesis (SE) and zygotic embryogenesis (ZE). Studies on ZE are limited, while SE research efforts are substantial but disjointed, potentially hindering efficient large-scale plant regeneration. Comprehensive and integrative studies of both SE and ZE are needed to address these challenges and provide a molecular foundation for plant embryogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Houssein Wehbi, Camille Soulhat, Halima Morin, Abdelhafid Bendahmane, Pierre Hilson, Oumaya Bouchabke-Coussa
Summary: Plant somatic embryogenesis (SE) is a natural process of vegetative propagation and can be induced in tissue cultures for various purposes. In this study, SE induction in monocots with Brachypodium distachyon as a model was investigated. The researchers identified the earliest stages of cell fate transition and demonstrated that secondary somatic embryos can develop within one week of in vitro culture. The fast SE induction method described in this study could contribute to the mechanistic study of SE processes and shorten the production of transgenic or gene-edited plants.
Article
Horticulture
HongYu Li, FengSong Liu, ShengLi Song, ChunXia Wang, HongMei Sun
Summary: In this study, an efficient in vitro regeneration protocol for Clivia miniata through somatic embryogenesis via a TCL technique was established. This protocol provides a reliable and stable system for sustainable commercial cultivation and conservation of Clivia bioresources.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Zurisadai Monroy-Gonzalez, Miguel A. Uc-Chuc, Ana O. Quintana-Escobar, Fatima Duarte-Ake, Victor M. Loyola-Vargas
Summary: Auxins play a significant role in plant development, and their movement is facilitated by complex transport systems. This study focused on the PIN transporters in Persea americana and found 12 different types of transporters expressed during embryo development. The researchers used bioinformatics tools to analyze the structure and potential phosphorylation sites of these PIN transporters, revealing conserved phosphorylation sites and sites important for IAA interaction.
Article
Plant Sciences
Jacobo Perez-Pastrana, Pilar S. Testillano, Ivett Barany, Adriana Canto-Flick, Dulce Alvarez-Lopez, Gema Pijeira-Fernandez, Susana A. Aviles-Vinas, Laura Pena-Yam, Liliana Munoz-Ramirez, Sara Nahuat-Dzib, Ignacio Islas-Flores, Nancy Santana-Buzzy
Summary: Zygotic and somatic embryogenesis in plants are finely regulated by a specific group of genes and dynamic levels of plant hormones, with levels of indole-3-acetic acid (IAA) increasing as embryogenesis progresses. The distribution of IAA changes dynamically throughout different stages of embryo development, showing a correlation between IAA levels and cell differentiation in Capsicum chinense species.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Horticulture
Nevena Banjac, Dijana Krstic-Milosevic, Tatjana Mijalkovic, Mirjana Petrovic, Tatjana Cosic, Mariana Stanisic, Branka Vinterhalter
Summary: In this study, an efficient tissue culture system for improving the rocket cultivar Domaca rukola was established. Optimal shoot multiplication and organogenesis protocols were determined through experiments with different hormone concentrations and combinations. In addition, in vitro-produced rocket shoots were found to be a potential source of the antioxidant flavonoid kaempferol.
Article
Plant Sciences
Concepcion avila, Maria Teresa Llebres, Vanessa Castro-Rodriguez, Cesar Lobato-Fernandez, Isabelle Reymond, Luc Harvengt, Jean-Francois Trontin, Francisco M. Canovas
Summary: This study compared the developmental processes of somatic and zygotic embryos in pines through genome-wide transcriptome analysis. The differences between the developmental stages and the effects on metabolic pathways were identified. Additionally, differentially expressed transcription factors in these two types of embryos were analyzed, and potential key regulatory genes were identified to provide insights into the regulation of this process.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: Plant micropropagation has been widely used in agriculture, horticulture, forestry, and related fields for large-scale production of high-quality plants. The use of liquid media and bioreactors has increased the yield of healthy plants. This article discusses the design, principle, operational mode, advantages, and disadvantages of various bioreactors used for plant propagation, as well as the parameters that affect plant regeneration with suitable examples.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Anatomy & Morphology
Martti P. Montanari, Ngan Vi Tran, Osamu Shimmi
Summary: Bone morphogenetic proteins (BMPs) play crucial roles in animal development, with highly conserved signals in the genetic toolkit and precise control at the post-translational level. Research focuses on the formation of BMP morphogen gradient and context-dependent control of BMP signal to facilitate complex tissue structure formation.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Plant Sciences
Renata Orlowska, Janusz Zimny, Piotr T. Bednarek
Summary: In this study, the role of copper ions as a mediator of DNA sequence variation in the CHG context was highlighted while silver ions did not show any mediating effect. Modifications in in vitro culture conditions explained DNA sequence variation, demethylation, and de novo methylation in the CHG context. Incorporating copper ions in the induction medium should be approached cautiously.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Tianyao He, Jinyu Peng, Shu Yang, Dongsong Liu, Shuang Gao, Yanlong Zhu, Zhuang Chai, Byeong Chun Lee, Renyue Wei, Jiaqiang Wang, Zhonghua Liu, Jun-Xue Jin
Summary: This study identifies a novel retrotransposon-associated long noncoding RNA (lncRNA), SAWPA, in porcine early embryos. SAWPA is essential for porcine embryonic development by regulating the JNK-MAPK signaling pathway.
Review
Developmental Biology
Aidan H. Terhune, Jeyoon Bok, Shiyu Sun, Jianping Fu
Summary: The development of a zygote into an embryo is a miraculous process in nature, but scientists have struggled to understand the mechanisms in human embryos due to ethical and practical limitations. However, pluripotent stem cells have the ability to mimic specific embryonic events, allowing the creation of in vitro models that recapitulate different stages of early embryo development.
Article
Plant Sciences
Kamila Godel-Jedrychowska, Katarzyna Kulinska-Lukaszek, Ewa Kurczynska
Summary: The research found that the symplasmic domains between somatic embryos (SEs) and zygotic embryos (ZEs) were similar, but there were spatial-temporal differences in the symplasmic transport restriction. These findings provide a new perspective on intercellular signaling during embryonic development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Fisheries
Mohammad Ashaf-Ud-Doulah, S. M. Majharul Islam, Md Mahiuddin Zahangir, Md Sadiqul Islam, Christopher Brown, Md Shahjahan
Summary: The study found that temperature has a significant impact on the development of rohu carp embryos and larvae, with the optimal temperature range being 30-32 degrees Celsius or lower. Higher temperatures disrupt embryonic metabolism and physiology, resulting in aberrant and unsuccessful early development.
AQUACULTURE INTERNATIONAL
(2021)
