Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 105, Issue 3, Pages 309-316Publisher
SPRINGER
DOI: 10.1007/s11240-010-9869-3
Keywords
Na+/H+ antiporter; Ammopiptanthus mongolicus; Salt tolerance; Drought tolerance
Funding
- Science and Technology Committee of the Shanghai Municipal Government [08DZ2270800]
- National Program of Transgenic Variety Development of China [2008ZX08009-004, 2009ZX08009-061B]
Ask authors/readers for more resources
An orthologue of the vacuolar Na+/H+ antiporter gene, AmNHX2, was isolated from a desert plant, Ammopiptanthus mongolicus, by RACE-PCR. It has a total length of 1,986 bp, with an open reading frame of 1,632 bp, encoding a predicted polypeptide of 543 amino acids. Sequence similarity and exon constituent analysis clearly suggested that AmNHX2 encoded an AtNHX2 (an antiporter from Arabidopsis) like vacuolar Na+/H+ antiporter. AmNHX2 could be strongly induced by both drought and salt stress. Heterologous expression in the yeast mutant nhx1 indicated that AmNHX2 was the orthologue of ScNHX1, and the complementation effect was almost the same as AtNHX1. Over-expressing AmNHX2 resulted in enhanced tolerances to both drought and salt stresses in transgenic Arabidopsis plants. The transgenic plants accumulated lower Na+ content in their leaves, showing healthier root system after salt stress, and retained more water during the drought stress. Our work suggested that AmNHX2 was a salt tolerance determinant in A. mongolicus, but might not be a contributor to the difference in salt sensitivity between A. thaliana and A. mongolicus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available