Article
Plant Sciences
Ying Peng, Rui Pan, Yi Liu, Milca Banda Medison, Abdullah Shalmani, Xinsun Yang, Wenying Zhang
Summary: This study identified differentially expressed mRNAs, miRNAs, and lncRNAs in two sweet potato genotypes with high and low CGA content, and explored the regulatory networks involved in CGA synthesis. The findings provide insights into the role of ncRNAs in CGA synthesis and its potential application in improving sweet potato's nutritional value.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Multidisciplinary Sciences
Weimin Zhang, Ran Brosh, Laura H. McCulloch, Yinan Zhu, Hannah Ashe, Gwen Ellis, Brendan R. Camellato, Sang Yong Kim, Matthew T. Maurano, Jef D. Boeke
Summary: This study presents a new conditional knockout strategy that enables efficient counter selection in mouse embryonic stem cells. The strategy overwrites counter selectable markers and allows the removal or replacement of wild-type DNA with the desired payload DNA. The restored native gene function in the conditional knockout cells facilitates subsequent mouse development and the generality of the strategy is demonstrated through the knockout of other genes.
Article
Multidisciplinary Sciences
Agnieszka M. Murakami, Katsuhiro Nagatomo, Ichro Miyoshi, Shirou Itagaki, Yasutaka Niwa, Manabu Murakami
Summary: A new method was developed to analyze protein-protein interactions using a dual-inducible prokaryotic expression system. This method involved constructing a chimeric fusion toxin gene to evaluate protein-protein binding. The study identified a new binding site in a voltage-dependent calcium channel.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Anna V. Levina, Owen A. Hoekenga, Mikhail Gordin, Corey Broeckling, Walter S. De Jong
Summary: In this study, network analysis was used to organize metabolic features detected by mass spectrometry into modules, as a precursor to genetic analysis. The results showed that features associated with chip color and chipping were often not clustered together in the network analysis, indicating the complexity of linkage disequilibrium in potato network analysis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Rungtiwa Nutalai, Daming Zhou, Aekkachai Tuekprakhon, Helen M. Ginn, Piyada Supasa, Chang Liu, Jiandong Huo, Alexander J. Mentzer, Helen M. E. Duyvesteyn, Aiste Dijokaite-Guraliuc, Donal Skelly, Thomas G. Ritter, Ali Amini, Sagida Bibi, Sandra Adele, Sile Ann Johnson, Bede Constantinides, Hermione Webster, Nigel Temperton, Paul Klenerman, Eleanor Barnes, Susanna J. Dunachie, Derrick Crook, Andrew J. Pollard, Teresa Lambe, Philip Goulder, Neil G. Paterson, Mark A. Williams, David R. Hall, Juthathip Mongkolsapaya, Elizabeth E. Fry, Wanwisa Dejnirattisai, Jingshan Ren, David Stuart, Gavin R. Screaton
Summary: This study compares the neutralization of Omicron variants of SARS-CoV-2 and finds that differences in neutralization mostly arise from variations in residues bordering the ACE2 binding site. Analysis of monoclonal antibodies isolated from vaccinated individuals shows that they can effectively neutralize early pandemic strains and exhibit broad reactivity with variants.
Article
Horticulture
Xiang Li, Chengcheng Lyu, Jun Song, Yifei Lu, Fuchun Zeng, Liming Lu, Liqin Li
Summary: In this study, 23 ADK genes were identified in potato using genome data, and a comprehensive study was conducted using bioinformatics methods. The results showed that these genes are involved in the regulation of cold, ABA, salt, H2O2 and drought stresses, providing a foundation for further understanding the molecular mechanism of potato ADK genes.
Article
Plant Sciences
Diogo Gil, Joana M. S. Cardoso, Isabel Abrantes, Ivania Esteves
Summary: The morphometry of five P. penetrans isolates parasitizing potato roots in Portugal was compared, with vulva position and stylet length showing the lowest variability within and between isolates.
DNA sequencing revealed higher genetic diversity in ITS region compared to COI gene, showing 15 and 7 haplotypes respectively. Intra- and inter-isolate genetic diversity was found in both genomic regions.
Article
Fisheries
Ke Liu, Jinxia Liu, Tianjiao Ren, Youhou Xu, Min Lu, Huaiyi Fang, Yan Zhang, Yongyan Liao, Peng Zhu
Summary: Azadirachtin is a botanical pesticide toxic to arthropods, while GST enzymes play a role in drug metabolism. Cloning of GSTM3, GSTT1, and GSTD1 genes in Eriocheir hepuensis revealed their expression patterns under azadirachtin stress, indicating the regulatory role of these genes in response to the pesticide.
AQUACULTURE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Panfeng Yao, Chunli Zhang, Tianyuan Qin, Yuhui Liu, Zhen Liu, Xiaofei Xie, Jiangping Bai, Chao Sun, Zhenzhen Bi
Summary: In this study, 19 StGH3 genes were isolated and characterized. Phylogenetic analysis and gene structure analysis revealed the evolutionary characteristics of StGH3 genes. The study also showed that StGH3 genes may be involved in the response to abiotic stress through hormonal signal pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yaoyao Ma, Tianqi Zhao, Xinyi Wu, Zhangping Yang, Yujia Sun
Summary: Long noncoding RNAs, such as CFL1-AS1, play important roles in gene expression and animal development. CFL1-AS1 positively regulates the expression of CFL1 gene and affects the expression of CFL2. It also promotes cell proliferation, inhibits apoptosis, and participates in autophagy.
Article
Plant Sciences
Haixiang Ruan, Xingxing Shi, Liping Gao, Arif Rashid, Yan Li, Ting Lei, Xinlong Dai, Tao Xia, Yunsheng Wang
Summary: Anthocyanins and proanthocyanidins are important flavonoids that have various industrial and pharmaceutical applications. In this study, four CsDFR genes were isolated from Camellia sinensis and their overexpression was analyzed. The results showed that CsDFR genes are closely related to the accumulation of catechins and PAs. Enzyme activity analysis revealed two CsDFR proteins have DFR activity, while the other two do not. Overexpression of CsDFRa and CsDFRc restored the phenotype of purple petiole and seed coat color in AtDFR mutants. Site-directed mutagenesis further identified two amino acid residues that play a key role in controlling DFR reductase activity. Our findings provide comprehensive characterization of CsDFRs and their important role in metabolic engineering.
HORTICULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Shuang Liu, Yan-chao Bian, Wan-lun Wang, Tong-Jia Liu, Ting Zhang, Yue Chang, Rui Xiao, Chuan-ling Zhang
Summary: By analyzing differentially expressed genes, we identified crucial hub genes involved in spermatogenesis and found that they may be associated with non-obstructive azoospermia and testicular germ cell tumors.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Shutao He, Xiaomeng Hao, Shuli He, Xiaoge Hao, Xiaonan Chen
Summary: This study identified 198 IbAP2/ERF transcription factors in sweet potato, classified into three groups (ERF, AP2, and RAV) based on phylogenetic analysis. The evolutionary analysis suggested that the expansion of this gene family in sweet potato may have been caused by tandem duplication. Furthermore, the study found that these genes play crucial roles in plant growth, development, and stress responses, with some showing specific expression patterns during sweet potato root development and under various abiotic stress conditions.
Article
Multidisciplinary Sciences
Majed Dasouki, Ayodeele Alaiya, Tanziel ElAmin, Zakia Shinwari, Dorota Monies, Mohamed Abouelhoda, Amjad Jabaan, Feras Almourfi, Zuhair Rahbeeni, Fahad Alsohaibani, Fahad Almohareb, Hazzaa Al-Zahrani, Francisco J. Guzman Vega, Stefan T. Arold, Mahmoud Aljurf, Syed Osman Ahmed
Summary: Autosomal recessive mutations in G6PC3 can lead to isolated and syndromic congenital neutropenia, which includes congenital heart disease and atypical inflammatory bowel disease. Comprehensive multi-omics analyses identified damaging effects of novel mutations in G6PC3 and MPL, as well as a unique molecular cytokine profile in patients with IBD. Liquid chromatography-mass spectrometry-based proteomics analysis revealed differential expression of key proteins linked to congenital heart disease and IBD in G6PC3-deficient plasma samples.
Article
Biochemistry & Molecular Biology
Shijie Ye, Dongjie Yin, Xiaoyan Sun, Qinyi Chen, Ting Min, Hongxun Wang, Limei Wang
Summary: In this study, the TbUGGT gene was cloned from Trapa bispinosa Roxb. and its functional analysis was conducted, laying the foundation for further research on the synthesis of β G in Escherichia coli.
