Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 100, Issue 2, Pages 175-181Publisher
SPRINGER
DOI: 10.1007/s11240-009-9634-7
Keywords
Sugarcane mosaic virus (SCMV); Sugarcane yellow leaf virus (ScYLV); Meristem culture; Meristem size; Plant proliferation
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The use of apical meristem culture for simultaneous virus elimination and shoot proliferation in sugarcane was assessed. Virus-free plants were propagated from Sugarcane mosaic virus and Sugarcane yellow leaf virus-infected material of the South African commercial cultivar, NCo376. A combination of thermotherapy by hot water treatment of stem sections (nodes) and subsequent germination of vegetative buds at 40A degrees C and optimal meristem size were key factors for the production of virus-free plants. Only meristems of 2 mm in length or of a smaller size (but > 0.5 mm) resulted in virus-free sugarcane. Shoot induction and proliferation via direct organogenesis were achieved on Murashige and Skoog nutrient medium supplemented with 0.1 mg l(-1) 6-benzyladenine and 0.015 mg l(-1) 6-furfurylaminopurine (KIN). The established protocol provides for the rapid proliferation of virus-free shoots from infected sugarcane plants and approximately 1,300 shoots were propagated from a single 2 mm meristem in 11 weeks. Plants remained virus-free when tested 12 months later.
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