Article
Biotechnology & Applied Microbiology
Ahmed Madi Waheed Al-Mayahi
Summary: The addition of polyamines and silver thiosulfate to the nutrient medium improves shoot organogenesis, rooting, and production of genetically stable date palm plants.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Agronomy
Doina Clapa, Monica Harta
Summary: In this study, an effective protocol for clonal propagation of cv. Cascade was established using cytokinins as the only plant growth regulators. The protocol included stages of initiation, multiplication, rooting, and acclimatization. Molecular markers were used to confirm the genetic uniformity of the in vitro raised plants with the mother plant.
Article
Horticulture
Fredy P. Carrera, Carlos Noceda, Maria G. Mariduena-Zavala, Jose A. Garcia, Omar Ruiz-Barzola, Juan M. Cevallos-Cevallos
Summary: The study found that changes in metabolite profiles during micropropagation of banana plants can affect the accumulation of apoplast components in dwarf plants, indicating somaclonal variations.
Article
Green & Sustainable Science & Technology
Aikaterini N. N. Martini, Maria Papafotiou
Summary: This study investigated the in vitro seed and clonal propagation of Anthyllis hermanniae L. for the first time, aiming to introduce this plant into the horticulture industry. The study found that seeds stored for 4 to 18 months can have a germination rate higher than 80% after scarification, and juvenile explants established more efficiently compared to adult ones. Therefore, this study provides important insights for the sustainable utilization of A. hermanniae in the horticulture industry.
Article
Agronomy
Simona Ancona, Giuseppe De Mastro, Maria M. Jenderek, Claudia Ruta
Summary: This study focused on the cultivation of artichoke through tissue culture techniques, finding that the best growth media were MS519-A and BM. Root induction was successfully achieved with the addition of cytokinin and auxin, and using arbuscular mycorrhizal fungi improved plant performance in greenhouse conditions.
Article
Forestry
Maurizio Capuana, Werther Guidi Nissim, Joshua D. Klein
Summary: We describe a micropropagation protocol for three ecotypes of S. acmophylla, which showed that the best results for shoot proliferation were obtained by culturing the shoot explants on Woody Plant Medium containing benzyladenine. Separating the shoot tip from the underlying internodes further enhanced proliferation.
Review
Plant Sciences
Chaimae Meyad, Fatima Henkrar, Nezha Bouhaddou, Lahsen Khabar
Summary: The Quercus genus plays an important role in improving the ecosystem in urban and forest environments. However, unregulated exploitation of Quercus species has led to deforestation in many areas. Reforestation through in vitro propagation of Quercus plantations is the best solution. Different plant parts can be cultured using Quercus micropropagation, such as buds, meristems, and shoot tips. Somatic embryogenesis provides the highest multiplication rate. However, issues like basal calluses, vitrification, tissue necrosis, and phenol accumulation may arise during in vitro multiplication. Various strategies, such as adding supplements to the culture medium, have been implemented to overcome these problems.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Biotechnology & Applied Microbiology
Monika Kaminska, Elwira Sliwinska
Summary: An effective micropropagation protocol for kale was developed, using shoot tips as explants and 6-benzylaminopurine as the most effective cytokinin for axillary shoots multiplication. Rooting was successful using indole-3-acetic acid, and the highest survival rate during acclimatization was achieved using a soil and perlite mixture. No changes in DNA content were observed, and the micropropagation of kale was successful without any phenotypic aberrations.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Agronomy
Behzad Kaviani, Azam Barandan, Alicja Tymoszuk, Dariusz Kulus
Summary: Pears are economically important fruits that are grown worldwide. This study investigated the in vitro propagation of P. communis L. using different growth media and hormone combinations. The combination of 2 mg center dot L-1 BA and 1 mg center dot L-1 Kin in MS medium showed the highest shoot proliferation, while the longest shoots were obtained in WPM enriched with 1 mg center dot L-1 BA. The highest number of roots was obtained on MS medium augmented with 1 mg center dot L-1 IAA. The acclimatization of the produced plantlets resulted in a 90% survival rate.
Article
Plant Sciences
M. Manokari, S. Priyadharshini, Mahipal S. Shekhawat
Summary: A micropropagation method for Coccoloba uvifera has been developed, achieving a 96% success rate in bud development from surface-sterilized nodal explants collected from a 9-year-old mature tree. This method can be applied for large scale production and sustainable conservation of this vulnerable species for agroforestry in coastal regions.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Felipe de Jesus Romo-Paz, Raquel Folgado, Lourdes Delgado-Aceves, Juan Francisco Zamora-Natera, Liberato Portillo
Summary: The study successfully developed an efficient method for mass multiplication of Physalis angulata and cryopreservation of apical meristems by droplet-vitrification. In vitro propagation and root induction experiments showed that specific hormone concentrations could achieve good proliferation and rooting effects.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Horticulture
Maria Teresa Martinez, Beatriz Cuenca, Fatima Mosteiro, Pablo Pineiro, Felipe Perez, Alejandro Solla, Elena Corredoira
Summary: The massive propagation of cork oak individuals tolerant to Phytophthora cinnamomi is a major challenge for cork production. In this study, resistance screening was conducted on seedlings obtained from a survived cork oak tree. The most vigorous seedlings were selected and successfully micropropagated using axillary budding. The optimized micropropagation method provides valuable insights for the propagation of this important tree species in forestry.
Article
Plant Sciences
Takeshi Hirakawa, Seia Tanno
Summary: In this study, it was found that the combined treatment of stem explants with appropriate concentrations of gibberellic acid (GA(3)) and cytokinin is effective for the propagation of hops through shoot branching.
Article
Biochemistry & Molecular Biology
Yucong Bai, Miaomiao Cai, Yuping Dou, Yali Xie, Huifang Zheng, Jian Gao
Summary: In this study, key gene families of the cytokinin pathway in Moso bamboo were systematically identified and analyzed. The cytokinin pathway in Moso bamboo was found to be closely related to rice and evolutionarily conservative. Tissue-specific expression patterns and co-expression patterns with auxin and gibberellin were observed for cytokinin family genes in Moso bamboo. Auxin and cytokinin crosstalk were shown to regulate Moso bamboo growth, and a key factor for this crosstalk, the auxin signaling factor PheARF52, was identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Xiaojian Xia, Han Dong, Yanling Yin, Xuewei Song, Xiaohua Gu, Kangqi Sang, Jie Zhou, Kai Shi, Yanhong Zhou, Christine H. Foyer, Jingquan Yu
Summary: The study reveals that plant hormones promote bud outgrowth in tomato by directly regulating BRANCHED1, demonstrating that BR signaling integrates multiple pathways controlling shoot branching, making local BR signaling in axillary buds a potential target for shaping plant architecture.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
A. Ghosh, A. U. Igamberdiev, S. C. Debnath
BIOLOGIA PLANTARUM
(2017)
Article
Horticulture
Samir C. Debnath
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2017)
Article
Agronomy
Sapan Tailor, Natalia V. Bykova, Abir U. Igamberdiev, Samir C. Debnath
GENETIC RESOURCES AND CROP EVOLUTION
(2017)
Article
Agriculture, Multidisciplinary
Cara K. Isaak, Pengqi Wang, Suvira Prashar, Karmin O, Daniel C. W. Brown, Samir C. Debnath, Yaw L. Siow
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2017)
Article
Agronomy
Juran C. Goyali, Abir U. Igamberdiev, Samir C. Debnath
CANADIAN JOURNAL OF PLANT SCIENCE
(2018)
Article
Multidisciplinary Sciences
Amrita Ghosh, Abir U. Igamberdiev, Samir C. Debnath
SCIENTIFIC REPORTS
(2018)
Review
Biochemistry & Molecular Biology
Samir C. Debnath, Juran C. Goyali
Article
Horticulture
Usha Arigundam, Asokan Mulayath Variyath, Yaw L. Siow, Dawn Marshall, Samir C. Debnath
SCIENTIA HORTICULTURAE
(2020)
Review
Agronomy
Samir C. Debnath, Usha Arigundam
Review
Biochemistry & Molecular Biology
Amrita Ghosh, Abir U. Igamberdiev, Samir C. Debnath
Summary: Plant tissue culture techniques are commonly used for commercial micropropagation, but may lead to genetic and epigenetic variations, including changes in DNA methylation pattern. These variations can impact plant phenotypes, and molecular techniques can be employed to detect DNA methylation. Understanding the molecular basis of DNA methylation can help in designing strategies to maintain clonal fidelity of regenerants in micropropagation systems.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Amrita Ghosh, Abir U. Igamberdiev, Samir C. Debnath
Summary: The study compared the DNA methylation levels of regenerants of two half-high blueberry cultivars grown in different culture systems, finding that plantlets developed from liquid medium showed higher levels of DNA methylation alterations.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biochemistry & Molecular Biology
Dhrumit S. Bhatt, Samir C. Debnath
Summary: Blueberries have been studied for their antioxidant properties, total phenolic and flavonoid contents, and genetic diversity using various markers. Different genotypes showed varied levels of antioxidant efficacy, with wild clones exhibiting higher variation in total phenolic and flavonoid contents. Molecular analysis revealed extensive genetic diversity, with certain markers associated with antioxidant properties. This study provides valuable information for breeding and germplasm conservation programs.
Article
Multidisciplinary Sciences
Arindam Sikdar, Umanath Sharma, Rajesh Barua, Abir U. Igamberdiev, Samir C. Debnath
Summary: Epigenetic variation and interaction between zeatin-induced cytosine methylation and secondary compounds play important roles in tissue culture and cutting propagation of lingonberry plants.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, General & Internal
Susara Madduma Hewage, Suvira Prashar, Samir C. Debnath, O. Karmin, Yaw L. Siow
FRONTIERS IN MEDICINE
(2020)
Article
Multidisciplinary Sciences
Samir C. Debnath, Dong An