Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 94, Issue 2, Pages 219-223Publisher
SPRINGER
DOI: 10.1007/s11240-008-9395-8
Keywords
Acidovorax; black locust; Dyella; endophyte; lemongrass oil; Microbacterium; micropropagation; Sphingomonas; thyme oil
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During tissue culture of black locust (Robinia pseudoacacia L.), serious problems with plant-associated bacteria led to a reduction of propagation potential in several clones. Four dominant strains of plant-associated bacteria could be isolated and were assigned to the genera Acidovorax, Dyella, Microbacterium and Sphingomonas. Out of five essential oils tested, thyme and lemongrass oil at a concentration of 0.03% each and 0.015% of both oils in combination clearly inhibited the growth of these bacteria strains on bacteriologic medium. There were no significant differences in total bacterial population density when penicillin, thyme and lemongrass oil or thyme plus lemongrass oil were added to the plant propagation media. The use of lemongrass oil changed the proportion of dominant bacterial strains.
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