Article
Plant Sciences
Guihua Duan, Chunqin Li, Yanfang Liu, Xiaoqing Ma, Qiong Luo, Jing Yang
Summary: This study utilized UHPLC/Q-TOF-MS to identify altered metabolites in transgenic rice infected with M. oryzae, revealing organic acids, organic oxygen compounds, lipids, and lipid-like molecules. The research showed that carbohydrate and amino acid metabolic pathways were involved in plant defense responses and resistance strengthening. The study also found that the identified metabolites could be utilized as an optimal marker for M.oryzae defense in rice.
Article
Plant Sciences
Yanfang Nie, Guanjun Li, Jieling Li, Xiaoshu Zhou, Yanzhi Zhang, Qingchuan Shi, Xiaofan Zhou, Huaping Li, Xiao-Lin Chen, Yunfeng Li
Summary: This study identified a novel elicitor-activity protein, MoVcpo, secreted by M. oryzae. It was found that MoVcpo acted both as a pathogen-associated molecular pattern (PAMP) to trigger rice immunity and as a virulence factor facilitating fungal infection. Additionally, MoVcpo was highly expressed in the early stage of M. oryzae infection, affecting various aspects of fungal development and sensitivity to stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Guihua Duan, Xiaoqing Ma, Zhufeng Shi, Yaqiong Yang, Hongfeng Chen, Qiong Huang, Jing Yang
Summary: MoSDT1, a transcription factor of rice blast fungus, acts as an inducer to activate defense response in rice mainly by mediating phosphorylated proteins. It plays a crucial role in enhancing rice resistance by modulating defense-related genes, ROS synthesis, callose deposition, PR gene expression, and SA/JA synthesis. MoSDT1 specifically up-regulates the expression levels of phosphorylated proteins involved in stress responses and growth in rice, highlighting its regulatory effects on protein phosphorylation.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Ecology
Kuleshwar Prasad Sahu, Aundy Kumar, Asharani Patel, Mukesh Kumar, S. Gopalakrishnan, G. Prakash, R. Rathour, Robin Gogoi
Summary: Dark brown necrotic lesions caused by Magnaporthe oryzae on rice foliage provide a contrasting microhabitat for leaf-colonizing microbiome, with 17 bacterial species identified in these lesions showing biocontrol potential against the pathogen. Over 50% of the bacterial isolates were able to suppress the growth of M. oryzae, with certain strains triggering the expression of defense genes in rice, indicating their potential as biocontrol agents for managing rice blast disease.
Article
Agronomy
Na Cui, Xiaofeng Chen, Yan Shi, Meirong Chi, Jiantuan Hu, Kunlong Lai, Zonghua Wang, Haifeng Wang
Summary: DNA methylation plays a role in regulating the expression of coding and non-coding regions in plants. A study using whole-genome bisulfite sequencing revealed temporal DNA methylation changes in rice after infection with Magnaporthe oryzae, showing a slight increase in global DNA methylation levels compared to Arabidopsis. Over 38,000 differentially methylated regions (DMRs) were identified, with hypermethylated DMRs dominating over hypomethylated DMRs, most of which were located in transposable element regions. Transcription of NBS-LRR family genes was affected by changes in DNA methylation, indicating the essential role of DNA methylation in rice response to M. oryzae infection.
Article
Plant Sciences
Kanyanat Lamanchai, Nicholas Smirnoff, Deborah L. Salmon, Athipat Ngernmuen, Sittiruk Roytrakul, Kantinan Leetanasaksakul, Suthathip Kittisenachai, Chatchawan Jantasuriyarat
Summary: This study investigates the role of the OsVTC1-1 gene in rice blast fungus inoculation and finds that the gene regulates rice blast resistance through multiple defense mechanisms, including hormone synthesis and signaling pathways.
Article
Biochemistry & Molecular Biology
Bellamkonda Ramakrishna, P. V. S. R. N. Sarma, Sravani Ankati, Bhoopal Bhuvanachandra, Appa Rao Podile
Summary: Long-chain chitooligosaccharides with degree of polymerization greater than 4 have potential biological activities. A mutant endo-chitinase was used to synthesize COS with DP6 and DP7, inducing defense response in rice seedlings and affecting gene expression levels, possibly through phosphorylation signaling pathways.
CARBOHYDRATE RESEARCH
(2021)
Article
Plant Sciences
Junning Ma, Jean-Benoit Morel, Michael Riemann, Peter Nick
Summary: This study dissects the JA-dependent defense signaling in rice for both compatible and incompatible interactions by using JA-deficient mutants cpm2 and hebiba. The results demonstrate the pivotal role of JA in the basal immunity of rice in its resistance to the Rice Blast Fungus in both types of interactions.
Article
Biochemistry & Molecular Biology
Shumin Ying, Zhen Zhang, Yanan Zhang, Zhongna Hao, Rongyao Chai, Haiping Qiu, Yanli Wang, Xueming Zhu, Jiaoyu Wang, Guochang Sun, Fucheng Lin
Summary: This study reveals the crucial role of DExD/H-box protein MoDHX35 in appressoria formation and virulence of the rice blast fungus, highlighting the involvement of DEAH-box proteins in the pathogenicity of plant fungal pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Enshuang Zhao, Hao Zhang, Xueqing Li, Tianheng Zhao, Hengyi Zhao
Summary: Studies have shown that fungi cause plant diseases through RNA interference mechanism and secreted protein infection mechanism. The coordination of sRNAs and proteins in the process of infection is not well understood. Utilizing transcriptomics and proteomics for studying the mechanism has important theoretical and practical value in controlling diseases and improving yields.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Zhiquan Liu, Jiehua Qiu, Zhenan Shen, Congcong Wang, Nan Jiang, Huanbin Shi, Yanjun Kou
Summary: Rice blast, caused by Magnaporthe oryzae, is a devastating disease that affects rice. This study investigates the interaction between the avirulence factor AvrPi9 and the resistance protein Pi9 in rice, as well as the role of AvrPi9 in promoting infection. The researchers found that AvrPi9 suppresses basal resistance against M. oryzae when expressed in a cultivar lacking Pi9. They also identified a protein named OsRGLG5 that interacts with AvrPi9 and positively regulates basal resistance against rice blast. This study provides insights into the mechanisms of rice resistance against blast and highlights the importance of understanding the interaction between pathogens and host plants.
PLANT COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang, Xinqiong Liu
Summary: The study aimed to investigate the regulation of gene expression in the interaction between rice and Magnaporthe oryzae using gene chip technology. Changes in gene expression at different time points post-inoculation were analyzed, revealing significant differentially expressed genes and identifying new genes related to rice blast. This research may contribute to the discovery of novel resistance genes and improve the understanding of rice blast development mechanisms.
Article
Plant Sciences
Lei Wei, Zhengying Shao, Yanhui Fu, Zhang Li, Guorong Ni, Xin Cheng, Yangping Wen, Saijin Wei
Summary: This study found that Streptomyces JD211 could protect rice from Magnaporthe oryzae damages by promoting signal transduction and inducing the production of phenylpropanoids. The results not only revealed the molecular mechanism involved in JD211-induced resistance, but also provided application clues for genetic breeding of crops with improved M. oryzae resistance.
JOURNAL OF PLANT INTERACTIONS
(2022)
Article
Cell Biology
Xiaoman You, Shanshan Zhu, Haowen Sheng, Zheng Liu, Dan Wang, Min Wang, Xiao Xu, Feng He, Hong Fang, Fan Zhang, Debao Wang, Zeyun Hao, Ruyi Wang, Yinghui Xiao, Jianmin Wan, Guo-Liang Wang, Yuese Ning
Summary: Knockdown of OsPEX5 enhances resistance to rice blast fungus Magnaporthe oryzae. OsPEX5 interacts with E3 ubiquitin ligase APIP6 to regulate plant immunity. Additionally, OsPEX5 interacts with OsALDH2B1 to enhance its repression of defense gene expression.
Article
Plant Sciences
Huajun Zhu, Hu Zhou, Zuohua Ren, Erming Liu
Summary: Bacillus subtilis JN005 shows growth promotion and biocontrol efficacy against rice blast, improving germination and growth of rice seeds, enhancing defense enzyme activities in rice leaves, and reducing disease incidence by 79% and 76% in curative and preventive treatments, respectively. Additionally, field experiments demonstrate that spraying with JN005 suspension at 1x10(7) cfu/mL can effectively control rice blast and increase rice production yield.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Mingjia Chen, Marco Herde, Claus-Peter Witte
Article
Biochemistry & Molecular Biology
Mingjia Chen, Mounashree J. Urs, Ismael Sanchez-Gonzalez, Monilola A. Olayioye, Marco Herde, Claus-Peter Witte
Article
Microbiology
Huaixing Shi, Hongmei Zeng, Xiufen Yang, Jing Zhao, Mingjia Chen, Dewen Qiu
CURRENT MICROBIOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
T. Zhou, H. Zeng, D. Qiu, X. Yang, B. Wang, M. Chen, L. Guo, S. Wang
JOURNAL OF APPLIED MICROBIOLOGY
(2011)
Correction
Biochemistry & Molecular Biology
Jing Zhao, Lihua Guo, Hongmei Zeng, Xiufen Yang, Jingjing Yuan, Huaixing Shi, Yehui Xiong, Mingjia Chen, Lei Han, Dewen Qiu
Article
Biochemistry & Molecular Biology
Jing Zhao, Lihua Guo, Hongmei Zeng, Xiufen Yang, Jingjing Yuan, Huaixing Shi, Yehui Xiong, Mingjia Chen, Lei Han, Dewen Qiu
Article
Multidisciplinary Sciences
Mingjia Chen, Hongmei Zeng, Dewen Qiu, Lihua Guo, Xiufen Yang, Huaixing Shi, Tingting Zhou, Jing Zhao
Article
Biochemistry & Molecular Biology
Mingjia Chen, Claus-Peter Witte
Article
Multidisciplinary Sciences
Changhua Zhu, Xiaoye Liu, Wenlei Wang, Xiaoguang Chen, Shangyu Gao, Meng Qian, Na Yang, Yifeng Xu, Mingjia Chen
Summary: This study presents a rapid and reliable method for absolute quantification of nucleoside/nucleotide content in plants. By utilizing external standards in HPLC-MS/MS, researchers can easily quantify nucleosides/nucleotides, making it suitable for different plant species.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Jinjiao Zuo, Yanan Cao, Zhisheng Wang, Abid Ullah Shah, Wenlei Wang, Chen Dai, Mingjia Chen, Jian Lin, Qian Yang
Summary: IBV inhibits the antigen-presentation ability of avian dendritic cells by suppressing their maturation, cytokine secretion, and antigen presentation.
Article
Biochemistry & Molecular Biology
Shangyu Gao, Yu Sun, Xiaoguang Chen, Changhua Zhu, Xiaoye Liu, Wenlei Wang, Lijun Gan, Yanwu Lu, Frank Schaarschmidt, Marco Herde, Claus-Peter Witte, Mingjia Chen
Summary: This study reveals that free 5-methylcytidine (5mC) and 5-methyl-2'-deoxycytidine are produced from nucleic acid turnover in Arabidopsis thaliana. The degradation process of these cytidines, which is unclear in eukaryotes, is elucidated. It is found that 5-methyluridine (5mU) is mainly generated from RNA, and most 5mU is directly released from RNA without a 5mC intermediate. The removal of 5mU is shown to be an important function of pyrimidine degradation in many organisms, protecting RNA from stochastic m(5)U modification in plants.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wenlei Wang, Huijie Liu, Feifei Wang, Xiaoye Liu, Yu Sun, Jie Zhao, Changhua Zhu, Lijun Gan, Jinping Yu, Claus-Peter Witte, Mingjia Chen
Summary: This study reveals the occurrence of N4-acetylation of cytidine in plant RNA and identifies two N-ACETYLTRANSFERASEs FOR CYTIDINE IN RNA (ACYR1 and ACYR2) that are essential for plant development. Mutations in these genes result in defects in leaf and embryo development due to reduced acetylation and destabilization of the TOUGH transcript. These findings highlight the critical role of N4-acetylation of cytidine in plant development.