Journal
PLANT CELL REPORTS
Volume 32, Issue 11, Pages 1807-1818Publisher
SPRINGER
DOI: 10.1007/s00299-013-1493-3
Keywords
CAM; Carbon metabolism; OEE; Oxidative stress; PEPC; PEPCK; RT-qPCR; Rubisco
Categories
Funding
- Fundacao para a Ciencia e Tecnologia (FCT) [PEst-OE/AGR/UI0240/2011]
- [SFRH/BD/43181/2008]
- Fundação para a Ciência e a Tecnologia [PEst-OE/AGR/UI0240/2011, SFRH/BD/43181/2008] Funding Source: FCT
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Proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under C3 and CAM-inducing metabolisms to obtain insight into the CAM-facultative metabolism and the relationship of CAM plants with oxidative stress. Pineapple is one of the most important tropical crops worldwide. The use of temporary immersion bioreactors for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are carefully acclimatized to natural environmental conditions, and a facultative C3/CAM metabolism in the first 2 months of growth is the characteristic of pineapple plants, depending on environmental conditions. We subjected two sets of micropropagated pineapple plants to C3 and CAM-inducing environmental conditions, determined by light intensity/relative humidity (respectively 40 mu mol m(-2) s(-1)/85 % and 260 mu mol m(-2) s(-1)/50 %). Leaves of pineapple plants grown under CAM-inducing conditions showed higher leaf thickness and more developed cuticles and hypodermic tissue. Proteomic profiles of several proteins, isoenzyme patterns and transcriptomic profiles were also measured. Five major spots were isolated and identified, two of them for the first time in Ananas comosus (OEE 1; OEE 2) and the other three corresponding to small fragments of the large subunit of Rubisco (LSU). PEPC and PEPCK were also detected by immunobloting of 2DE at the end of both ex vitro treatments (C3/CAM) during the dark period. Isoenzymes of SOD and CAT were identified by electrophoresis and the transcript levels of OEE 1 and CAT were associated with CAM metabolism in pineapple plants.
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