Article
Horticulture
Jianfeng Liu, Jianying Liu, Xingzheng Zhang, Heng Wei, Jiahua Ren, Cheng Peng, Yunqing Cheng
Summary: The intermittent growth pattern of hazel pollen tubes has a significant impact on fruit cluster yield. Ca2+ and ALA treatments affect pistillate inflorescence drop and fruit production differently by influencing pollen tube growth. ChACA1 may play a key role in regulating pollen germination and tube development in hazel.
SCIENTIA HORTICULTURAE
(2021)
Article
Plant Sciences
Yiling Cai, Chao Tang, Shouzheng Lv, Qiming Chen, Xiaoxuan Zhu, Xian Li, Kaijie Qi, Zhihua Xie, Shaoling Zhang, Peng Wang, Juyou Wu
Summary: The study identifies the GAUT gene family and its role in controlling pollen tube growth in 8 Rosaseae species. PbrGAUT22 is found to regulate the content of pectins, thus influencing pear pollen tube growth.
Article
Plant Sciences
Zhongheng Xia, Binxu Wen, Jing Shao, Tianci Zhang, Mengmeng Hu, Lin Lin, Yiping Zheng, Zhixin Shi, Xinlin Dong, Juanjuan Song, Yuanshan Li, Yongjie Wu, Yafang Yuan, Juyou Wu, Qingxi Chen, Jianqing Chen
Summary: In this study, the researchers investigated the mechanism of callose synthesis in pear pollen tubes and found that a specific gene, PbrCalS1B.1, plays a crucial role in regulating this process. They also discovered that another gene, PbrbZIP52, positively affects the longevity of pear pollen tubes by promoting callose synthesis. This finding has potential applications in breeding high-yielding pear cultivars and improving fruit setting in commercial orchards.
Article
Plant Sciences
Yazhou Duan, Limin Wang, Xueling Li, Wanlei Wang, Jing Wang, Xiaoyu Liu, Yangyang Zhong, Nana Cao, Mengjuan Tong, Weina Ge, Yi Guo, Rui Li
Summary: SKS11 and SKS12 are crucial for regulating the integrity, growth, and guidance of pollen tubes, possibly by modulating ROS levels and the deposition or remodeling of cell wall polysaccharides.
Article
Horticulture
Lei Wu, Ying Xu, Min He, Xue-Ting Jiang, Kai-Jie Qi, Chao Gu, Shao-Ling Zhang
Summary: ABRE-binding factors play a crucial role in gametophytic self-incompatibility in pear by activating genes responsive to the GSI reaction. RNA-Seq analysis revealed a large number of differentially expressed genes between self- and cross-pollinated styles, including 278 genes associated with the GSI reaction.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Xiaoxuan Zhu, Chao Tang, Qionghou Li, Xin Qiao, Xian Li, Yilin Cai, Peng Wang, Yangyang Sun, Hua Zhang, Shaoling Zhang, Juyou Wu
Summary: This study characterized the pectin methylesterase inhibitor gene family in seven Rosaceae species and identified three genes that regulate pollen tube growth in pear. Quantitative real-time PCR analysis revealed that these genes are increasingly expressed during pear pollen tube growth. The results suggest that pectin methylesterase inhibitor regulates pollen tube growth by changing the distribution of methylesterified pectin in the apex.
Article
Plant Sciences
Zhenguo Zhou, Sheng Zheng, Syed Inzimam Ul Haq, Dianfeng Zheng, Quan-Sheng Qiu
Summary: This review summarizes the current advances in understanding the function and mechanisms of cellular pH and ions in regulating pollen tube growth. It is found that ions such as Ca2+, K+, and Cl-, as well as cellular pH, play important physiological roles in pollen tube growth. Ca2+ is particularly crucial in regulating cytoskeletons and cell wall development. Furthermore, pH regulation of ion flow, cell wall development, auxin signaling, and cytoskeleton function also contribute to pollen tube growth. Unanswered questions regarding the interplay of ion homeostasis and pH dynamics, as well as the regulation of pollen tube growth by proton pumps and ion transporters in the trans-Golgi network, need to be addressed in the future.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Nutrition & Dietetics
Nicolette W. de Jong, Severina Terlouw, Frank E. van Boven, M. S. van Maaren, Marco W. J. Schreurs, Dianne B. P. M. Van den Berg-Somhorst, Diederik Esser, Shanna Bastiaan-Net
Summary: The study aimed to measure allergic symptoms in birch pollen allergic patients during challenges with two different pear varieties. Results showed that symptoms were less severe during challenges with 'Cepuna' pears, suggesting that sensitized patients may consume small amounts of 'Cepuna' pears outside of the birch pollen season.
Article
Plant Sciences
Lei Wu, Xing Liu, Ming-Yue Zhang, Kai-Jie Qi, Xue-Ting Jiang, Jia-Long Yao, Shao-Ling Zhang, Chao Gu
Summary: Through transcriptome and real-time PCR analyses, two leucine-rich repeat extensin genes, PbLRXA2.1 and PbLRXA2.2, were identified in pear. These genes were found to be significantly upregulated in pollen grains and pollen tubes of the self-compatible cultivar, 'Jinzhui', compared to the self-incompatible cultivar, 'Yali'. PbLRXA2.1 and PbLRXA2.2 promoted pollen tube growth and attenuated the inhibitory effects of self S-RNase, indicating their involvement in the loss of self-incompatibility. The promoters of these two genes were directly bound by the ABRE-binding factor PbABF.D.2, and knockdown of PbABF.D.2 inhibited pollen tube growth. Additionally, self S-RNase repressed the expression of PbLRXA2.1, PbLRXA2.2, and PbABF.D.2, suggesting its role in arresting pollen tube growth by restricting the PbABF.D.2-PbLRXA2.1/PbLRXA2.2 signal cascade.
Article
Chemistry, Multidisciplinary
Naoki Yanagisawa, Elena Kozgunova, Tetsuya Higashiyama
Summary: This study presents a microfluidic injector device coupled with a cell culture chamber to study directional cellular growth triggered by ligand-receptor interactions at single molecule resolution. The unidirectional fluidic flow introduced by a syringe pump, along with an on-chip electro-osmotic pump for temporal change in flow direction, allows for controlled transfer of sample plugs into the cell culture chamber. This setup potentially enables study of cellular responses to injected ligands at high spatiotemporal resolution.
Article
Biochemistry & Molecular Biology
Vladislav Chubinskiy-Nadezhdin, Svetlana Semenova, Valeria Vasileva, Alla Shatrova, Natalia Pugovkina, Yuri Negulyaev
Summary: Endometrial mesenchymal stem cells (eMSCs) have the ability to differentiate into different cell types and are influenced by mechanical forces in their microenvironment. This study identified Piezo1 proteins as mechanosensitive channels in the plasma membrane of eMSCs, which transduce mechanical stimuli into intracellular signaling pathways. The influx of Ca2+ triggered by Piezo1 activity is regulated by store-operated Ca2+ entry (SOCE) through the ORAI1 channel and STIM1/STIM2 Ca2+ sensors. Activation of Piezo1 or SOCE does not cause cytotoxicity in eMSCs but affects their migratory capacity and cell proliferation. This finding suggests that Piezo1 and SOCE play important roles in the regulation of intracellular calcium, which influences the migratory activity and regenerative potential of eMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Rafiquel Sarker, Ruxian Lin, Varsha Singh, Mark Donowitz, Chung -Ming Tse
Summary: In polarized intestinal epithelial cells, the downregulated in adenoma (DRA) is involved in both NaCl absorption and anion secretion. Forskolin (FSK) and adenosine 50-triphosphate (ATP) can stimulate the activity of DRA in a concentration-dependent manner. The synergistic increase of intracellular Ca2+ is essential for the stimulation of DRA by FSK and ATP.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Jingzhe Guo, Jiangman He, Katayoon Dehesh, Xinping Cui, Zhenbiao Yang
Summary: This study used a molecular toolset (CamelliA lines) in Arabidopsis to simultaneously and high-resolution monitor calcium dynamics in multiple subcellular compartments. Different calcium signatures were uncovered in Arabidopsis cells in response to developmental and external cues, including rapid oscillations of cytosolic calcium, apical plasma membrane calcium influx, and shockwave-like calcium waves propagating in laser-wounded leaf epidermis.
Review
Plant Sciences
Cai Yu Yu, Huan Kai Zhang, Ning Wang, Xin-Qi Gao
Summary: GPI-APs play important roles in the interactions between pollen/pollen tube and pistil tissues, affecting pollen germination on the stigma, pollen tube growth in the transmitting tract, pollen tube guidance to the ovule, and pollen tube reception in the embryo sac.
Review
Pharmacology & Pharmacy
Dagmar Wachten, David U. Mick
Summary: Primary cilia play a crucial role in sensing extracellular signals and regulating cellular processes. G-protein coupled receptors are key in primary cilia, and can be targeted by pharmacology, chemogenetics, and optogenetics.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Hao Ai, Xiuli Liu, Zhi Hu, Yue Cao, Nannan Kong, Feiyan Gao, Siwen Hu, Xing Shen, Xianzhong Huang, Guohua Xu, Shubin Sun
Summary: OsLPR3 is induced under phosphate deprivation and its overexpression inhibits the growth and development of rice without affecting phosphate homeostasis. However, oslpr3 mutants show improved root system architecture and phosphate utilization, as well as higher tolerance to low phosphate stress. OsLPR3 plays different roles from OsLPR5 in rice growth and development, as well as in maintaining phosphate status.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yafei Sun, Qin Qin, Ke Song, Lijuan Sun, Tingting Jiang, Shiyan Yang, Zhouwen Li, Guohua Xu, Shubin Sun, Yong Xue
Summary: This study reveals that OsSQD1 in rice is highly expressed in lateral roots under different phosphorus conditions, and its mutation inhibits the formation and growth of lateral roots. Additionally, the mutation of OsSQD1 changes the composition of different lipid species and decreases the concentration of phospholipids and glycolipids in phosphorus-starved roots. Therefore, OsSQD1 plays a crucial role in maintaining the phospholipid and glycolipid composition in phosphorus-deprived rice roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Kangning Li, Shuo Tang, Shunan Zhang, Yanghao Tian, Hongye Qu, Mian Gu, Guohua Xu
Summary: The circadian clock regulator Nhd1 in rice activates the expression of the sucrose transporter gene OsSUT1, leading to changes in carbon and nitrogen balance, and subsequently affecting photosynthesis rate, C to N ratio, and free amino acids. This in turn influences rice growth and development.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Shichao Wang, Tingting Xu, Min Chen, Liyan Geng, Zhaoyang Huang, Xiaoli Dai, Hongye Qu, Jun Zhang, Huanhuan Li, Mian Gu, Guohua Xu
Summary: A WRKY transcription factor, OsWRKY10, acts as a negative regulator by suppressing the expression of OsPHT1;2 gene in rice, thereby inhibiting phosphate uptake from phosphate-replete environments.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Tingting Chen, Shijia Lin, Ziping Chen, Tianyuan Yang, Shupei Zhang, Jinsong Zhang, Guohua Xu, Xiaochun Wan, Zhaoliang Zhang
Summary: A study has found that theanine, synthesized by GSI-like proteins in tea plants, plays a crucial role in nitrogen signaling and development. Exogenous theanine inhibits lateral root development by reducing H2O2 accumulation. This study reveals the regulatory role of CsTSI-synthesized theanine in lateral root development in tea plants.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Ying Wang, Huimin Feng, Jia Du, Xinxin Liu, Haiya Wang, Xiaoli Dai, Guohua Xu, Ling Yu
Summary: In this study, it was found that HtVHA-c and HtNHX2 have a synergistic function in regulating the pH of endosomes, improving plant mineral nutrition and salt tolerance.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Engineering, Environmental
Hong-Xiang Zheng, Wen-Shen Liu, Dan Sun, Shi-Chen Zhu, Yang Li, Yu-Lu Yang, Ruo-Rong Liu, Hua-Yuan Feng, Xuan Cai, Yue Cao, Guo-Hua Xu, Jean Louis Morel, Antony van der Ent, Lena Q. Ma, Yao-Guang Liu, Elizabeth L. Rylott, Rong-Liang Qiu, Ye-Tao Tang
Summary: Rare earth elements (REEs) are essential for modern technologies, but their production is resource-intensive and environmentally damaging. Certain plants can hyperaccumulate REEs, and understanding the biology behind this phenomenon could help in developing more environmentally friendly REE recovery technologies.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Plant Sciences
Nan Guo, Hongye Qu, Yue Zhi, Yuyi Zhang, Shujing Cheng, Jinfang Chu, Zhengguang Zhang, Guohua Xu
Summary: Plant amino acid transporters play crucial roles in regulating long-distance nitrogen transport, amino acid allocation, and defense against invading pathogens. However, the specific function of amino acid transporters in plant defense against pathogen infection remains unknown. In this study, researchers discovered that the rice amino acid transporter gene OsLHT1 is expressed in leaves and is up-regulated in response to maturation, nitrogen starvation, and infection by the rice blast fungus Magnaporthe oryzae. Knockout of OsLHT1 resulted in premature leaf senescence and sustained rusty red spots on mature leaves, regardless of nitrogen supply. Disruption of OsLHT1 also affected the transport and metabolism of amino acids, as well as the biosynthesis of flavones and flavonoids, leading to increased expression of defense genes, production of jasmonic acid and salicylic acid, and accumulation of reactive oxygen species. Importantly, inactivation of OsLHT1 significantly inhibited leaf invasion by M. oryzae. These findings establish OsLHT1 as a key regulator in the crosstalk between leaf appearance and resistance to rice blast fungus by mediating leaf amino acid homeostasis, ROS accumulation, and JA and SA production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Qingchun Zhao, Dechao Zeng, Zhenzhen Luo, Aiqun Chen, Guohua Xu, Yiting Li
Summary: Phosphorus deficiency increases flavonoid accumulation in tobacco shoots and up-regulates the expression of genes involved in flavonol biosynthesis. External application of quercetin, a flavonol, reduces soluble phosphate concentration but enhances phosphate starvation response in tobacco roots. Quercetin application also affects auxin response and up-regulates genes encoding putative phosphate transporters and purple acid phosphatases in tobacco roots. These findings suggest that P deficiency promotes flavonoid biosynthesis, and flavonoids may mediate the regulation of phosphate uptake and starvation signaling via the auxin pathway.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Pengyuan Gu, Wenqing Tao, Jinyuan Tao, Huwei Sun, Ripeng Hu, Daojian Wang, Guoxinan Zong, Xiaonan Xie, Wenyuan Ruan, Guohua Xu, Keke Yi, Yali Zhang
Summary: Phosphate fertilisers are essential for modern agriculture to achieve high yields. Understanding how plants sense and adapt to phosphate is crucial for improving phosphorus-use efficiency and promoting sustainable agriculture. Research has found that strigolactones play a role in regulating rice root development and metabolic adaptations to low phosphate, enhancing efficient phosphate uptake and translocation. This discovery sheds light on the crosstalk mechanism between strigolactone and phosphate signalling networks, which can be used to breed high-phosphorus-use efficiency crop plants.
Review
Plant Sciences
Yucong Li, Yu Chen, Yansong Fu, Jiahui Shao, Yunpeng Liu, Wei Xuan, Guohua Xu, Ruifu Zhang
Summary: This review summarizes the rhizosphere microbial signals that modulate plant root-system architecture and the underlying signaling mechanisms, and considers their potential use in agricultural production. Every living organism on Earth depends on its interactions with other organisms. In the rhizosphere, plants and microorganisms constantly exchange signals and influence each other's behavior. Recent studies have shown that many beneficial rhizosphere microbes can produce specific signaling molecules that affect plant root-architecture and therefore could have substantial effects on above-ground growth. This review examines these chemical signals and summarizes their mechanisms of action, with the aim of enhancing our understanding of plant-microbe interactions and providing references for the comprehensive development and utilization of these active components in agricultural production. In addition, we highlight future research directions and challenges, such as searching for microbial signals to induce primary root development.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Engineering, Civil
Hao Xu, Yide Shan, Ning Ling, Lixuan Ren, Hongye Qu, Zhipeng Liu, Guohua Xu
Summary: The application of hydrochar derived from food waste as a soil amendment can effectively improve soil structure and hydraulic properties, as well as increase soil water holding capacity. However, attention should be paid to the hydrophobicity of the hydrochar and its negative effects on plant growth.
JOURNAL OF HYDROLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Ying Liu, Guohua Xu
Article
Plant Sciences
Wenqi Zhang, Qi Meng, Wei Liu, Pinzhu Qin, Bowen Li, Guohua Xu
Summary: Plants respond to phosphate starvation stress by increasing carbohydrate accumulation and shoot-to-root transport, which activates signaling pathways. Overexpression of the Ugp1 gene decreases plant biomass, increases sucrose content, and promotes phosphate accumulation.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2023)
Article
Biochemistry & Molecular Biology
Junyu Wu, Shuaiqi Yang, Nana Chen, Qining Jiang, Linli Huang, Jiaxuan Qi, Guohua Xu, Lisha Shen, Hao Yu, Xiaorong Fan, Yinbo Gan
Summary: Nitrate is an important regulator of plant growth and development. In this study, the researchers investigated the mechanism by which the protein OsMADS25 moves from the cytoplasm to the nucleus and activates target genes involved in root growth in response to nitrate signals. The results showed that OsMADS25 moves to the nucleus in the presence of nitrate in a manner dependent on the protein OsNAR2.1. It directly activates the expression of OsMADS27 and OsARF7, which are known to be associated with root growth. These findings provide new insights into the regulation of root growth by nitrate signaling.
PLANT COMMUNICATIONS
(2023)
