Article
Plant Sciences
Lennart Hoengenaert, Jan Van Doorsselaere, Ruben Vanholme, Wout Boerjan
Summary: This study explores the delivery of gene-editing reagents into poplar trees using DNA-coated microparticle bombardment and evaluates its potential for developing transgene-free, gene-edited trees. The results show that the optimized transformation method can regenerate gene-edited trees without transgenes and antibiotic resistance-encoding genes, and donor DNA fragments can frequently integrate at specific target sites, opening opportunities for targeted gene insertions.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Yuan Liang, Xuehui Yan, Jingyi Xu, Yanlong Liu, Ke Xie, Jieqin Li, Qiuwen Zhan
Summary: In this study, a highly efficient microprojectile transformation system was developed for tannin-containing sorghum by using immature embryos of Hongyingzi as explants. After optimization, a transformation frequency of 27% was achieved and molecular analysis confirmed the presence and expression of transgenes in all transgenic plants. Inheritance analysis further demonstrated the inheritance of transgenes into the next generation, demonstrating the successful establishment of an efficient transformation system for tannin-containing sorghum to overcome the restriction imposed by tannins.
Article
Environmental Sciences
Xuexue Cao, Fanna Kong, Bin Sun, Jiqiang Yin, Hongzhong Ren, Huijie Yue, Chenggong Yu, Xianghai Tang, Guoying Du, Dongmei Wang, Yunxiang Mao
Summary: This study developed an efficient transformation toolbox for Neopyropia yezoensis, allowing the stable expression of foreign genes in its cells. The researchers found the optimal transformation conditions and identified an endogenous promoter that efficiently controls gene expression. This provides a strong foundation for functional genomics research and molecular genetic breeding on N. yezoensis.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemical Research Methods
Lap Man Lee, George J. J. Klarmann, Dustin W. W. Haithcock, Yi Wang, Ketan H. H. Bhatt, Balabhaskar Prabhakarpandian, Kapil Pant, Luis M. M. Alvarez, Eva Lai
Summary: Human adipose tissue is a valuable source of mesenchymal stem cells (MSCs) and a microfluidic sorter cascade has been developed to enrich and purify adipose-derived stem cells (ADSCs) from tissue-digested samples. The sorter cascade utilizes spiral-shaped inertial and deterministic lateral displacement (DLD) sorters to separate cells based on size difference, resulting in a >90% separation efficiency. The enriched ADSC sub-population shows a 6x enhancement of expansion capacity in tissue culture. This microfluidic sorter cascade holds promise for various applications in tissue engineering and regeneration medicine.
Article
Biology
Nader R. Abdelsalam, Wafaa E. Grad, Nabawya S. A. Ghura, Ahmed E. Khalid, Rehab Y. Ghareeb, El-Sayed M. Desoky, Mostafa M. Rady, Hatim M. Al-Yasi, Esmat F. Ali
Summary: Tissue culture methods were used to assess sugarcane genotypes' response to drought stress, with GT 54-9 genotype showing the highest tolerance based on all studied traits. This study highlights the potential of tissue culture techniques in understanding plant stress responses and identifying tolerant genotypes.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Wenbin Su, Mingyue Xu, Yasmina Radani, Liming Yang
Summary: Genetic transformation is a vital strategy for improving plant biomass and resistance to adverse environments, achieved through imparting desirable genetic characteristics. Research on plant genetic transformation technology can lead to functional analysis of plant genes, efficient utilization of desirable traits, and precise breeding. Various genetic transformation technologies have been developed, including direct methods like Agrobacterium-mediated gene delivery and indirect methods such as particle bombardment, electroporation, silicon carbide whiskers, and pollen tubes. The regeneration of transformed plants usually involves de novo organogenesis or somatic embryogenesis pathways. This review provides an overview of various techniques for plant genetic transformation, their application scope, and limiting factors, serving as a reference for plant transgenic breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Yuelin Liu, Weifeng Luo, Qianyan Linghu, Fumitaka Abe, Hiroshi Hisano, Kazuhiro Sato, Yoko Kamiya, Kanako Kawaura, Kazumitsu Onishi, Masaki Endo, Seiichi Toki, Haruyasu Hamada, Yozo Nagira, Naoaki Taoka, Ryozo Imai
Summary: The study discusses the limitations of genome editing technologies on elite wheat varieties and introduces a new in planta genome editing method using particle bombardment. Results show successful genome editing in commercially relevant Japanese elite wheat varieties through this method, demonstrating its potential for application in different types of elite wheat cultivars.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Lei Shi, Xiufang Cui, Jian Li, Guo Jin, Xuerun Zhang, Xin Wen, Haoliang Tian
Summary: In this study, the wear resistance and impact toughness of the carburized layer were improved through rare earth doped supersonic fine particle bombardment pretreatment assisted vacuum carburizing. CeO2 doped SFPB vacuum carburizing layer showed the highest wear resistance with the minimum wear rate.
MATERIALS CHARACTERIZATION
(2022)
Article
Multidisciplinary Sciences
X. Li, B. Bakhit, M. P. Johansson Joesaar, I Petrov, L. Hultman, G. Greczynski
Summary: This study proposes a novel approach for reducing energy consumption during thin film growth by combining high power impulse and direct current magnetron sputtering. The use of heavy metal-ion irradiation enables the growth of high-quality dense films at lower temperatures, resulting in significant energy savings and the ability to coat temperature-sensitive substrates.
SCIENTIFIC REPORTS
(2022)
Review
Plant Sciences
Jerome Jeyakumar John Martin, Rajesh Yarra, Lu Wei, Hongxing Cao
Summary: Oil palm, as a cross-pollinated crop with long generation time, faces challenges in achieving sustainable high-yield and high-quality production. Modern breeding tools, such as genomics, marker-assisted breeding, genetic engineering, and genome editing techniques, can be utilized to improve breeding methods in oil palm and develop new varieties with high yield potential and yield stability.
Article
Automation & Control Systems
Lei Shi, Jinna Liu, Jian Li, Zuo Wang, Xiufang Cui, Guo Jin, Haoliang Tian
Summary: A plastic deformation layer was fabricated on the surface of low carbon steel using supersonic fine particle bombardment, which refined the surface microstructure, increased hardness and residual stress, and improved hydrophobicity and wear resistance.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2022)
Article
Forestry
Phillip D. Jones, Autumn E. Sabo, Jodi A. Forrester, David J. Mladenoff, Marc E. McDill
Summary: This study examines the effects of deer on the survival and growth of sugar maple and ash seedlings in a Northern hardwood forest. The results show that the survival of seedlings is positively correlated with initial root collar diameter and is higher in transition zones. The study also finds that soil nitrogen positively influences the height growth of sugar maple but has no effect on ash growth.
FOREST ECOLOGY AND MANAGEMENT
(2023)
Article
Plant Sciences
Yaqiong Wang, Jian Zeng, Peipei Su, Hongyan Zhao, Li Li, Xiaoxue Xie, Qian Zhang, Ya'nan Wu, Ruibin Wang, Yufan Zhang, Boju Yu, Mingjie Chen, Yuesheng Wang, Guangxiao Yang, Guangyuan He, Junli Chang, Yin Li
Summary: This article provides a detailed protocol and experience for producing transgenic wheat using particle bombardment-mediated method, highlighting its advantages in genotype dependency and vector construction. The method has been successfully applied to multiple wheat genotypes and shows great potential and wide applications.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yanlin Yang, Na Li, Wenrong Hui, Binjie Yuan, Pan Fan, Jingxia Liu, Honggang Wang, Deshun Feng
Summary: This study showed that the specific expression of TaYUC10.3 in wheat young seeds could increase the content of auxin and protein, potentially affecting the synthesis pathway of auxin and the protein content of seeds.
PLANT CELL REPORTS
(2021)
Article
Multidisciplinary Sciences
Lihua Wang, Li Gao, Guoquan Liu, Ruirui Meng, Yanlong Liu, Jieqin Li
Summary: Efficient genetic transformation system using mature seeds as explants for sorghum has been established, overcoming limitations of relying on immature embryos, and confirmed heritability of transgenic plants through PCR and RTPCR analysis. This system will promote sorghum research on genetic engineering and genome editing without seasonal weather conditions and explant resources restrictions.
Article
Plant Sciences
Richard Moyle, Lara-Simone Pretorius, Jessica Dalton-Morgan, Denis Persley, Peer Schenk
AUSTRALASIAN PLANT PATHOLOGY
(2016)
Article
Plant Sciences
Richard L. Moyle, Lilia C. Carvalhais, Lara-Simone Pretorius, Ekaterina Nowak, Gayathery Subramaniam, Jessica Dalton-Morgan, Peer M. Schenk
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Plant Sciences
Jonni H. Koia, Richard L. Moyle, Jose R. Botella
Article
Plant Sciences
Ting-Chun Chou, Richard L. Moyle
Article
Biotechnology & Applied Microbiology
Stephen R. Mudge, Shiromi W. V. Basnayake, Richard L. Moyle, Kenji Osabe, Michael W. Graham, Terence E. Morgan, Robert G. Birch
PLANT BIOTECHNOLOGY JOURNAL
(2013)
Article
Biochemistry & Molecular Biology
Jonni Koia, Richard Moyle, Caroline Hendry, Lionel Lim, Jose Ramon Botella
PLANT MOLECULAR BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Richard L. Moyle, Robert G. Birch
PLANT MOLECULAR BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Richard L. Moyle, Jonni H. Koia, Julia Vrebalov, James Giovannoni, Jose R. Botella
PLANT MOLECULAR BIOLOGY
(2014)
Article
Biochemical Research Methods
Richard L. Moyle, Peter R. Sternes, Robert G. Birch
PLANT MOLECULAR BIOLOGY REPORTER
(2015)
Article
Biochemical Research Methods
Peter R. Sternes, Richard L. Moyle
PLANT MOLECULAR BIOLOGY REPORTER
(2015)
Article
Agronomy
Richard L. Moyle, Robert G. Birch
THEORETICAL AND APPLIED GENETICS
(2013)
Article
Plant Sciences
Richard L. Moyle, Jose R. Botella
TROPICAL PLANT BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Armand Cave-Radet, Armel Salmon, Loup Tran Van Canh, Richard L. Moyle, Lara-Simone Pretorius, Oscar Lima, Malika L. Ainouche, Abdelhak El Amrani
Summary: Environmental contamination by xenobiotics poses a significant threat to natural ecosystems and public health. The molecular mechanisms underlying xenobiotic detoxification remain poorly understood in plants, particularly in response to allopolyploidy. This study identified phenanthrene-responsive microRNAs in Spartina in the context of allopolyploid speciation, and demonstrated the relative impacts of hybridization and genome doubling in miRNA-guided regulation mechanisms in response to xenobiotics.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Lara Pretorius, Richard L. Moyle, Jessica Dalton-Morgan, Mark W. Schwinghamer, Kathy Crew, Peer M. Schenk, Andrew D. W. Geering
AUSTRALASIAN PLANT PATHOLOGY
(2017)