Journal
PLANT CELL AND ENVIRONMENT
Volume 37, Issue 9, Pages 2201-2210Publisher
WILEY
DOI: 10.1111/pce.12297
Keywords
Arabidopsis; mesophyll cells; white light
Categories
Funding
- program for New Century Excellent Talents in University [NCET-10-0128]
- National Science Foundation of China [30970266, 30670173, 31370319]
- Excellent Youth Foundation of Hebei Scientific Committee [C2010000411]
- Ministry of Education, Culture, Sports, Science and Technology [22570039, 19060003]
- Grants-in-Aid for Scientific Research [22570039] Funding Source: KAKEN
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Controversies regarding the function of guard cell chloroplasts and the contribution of mesophyll in stomatal movements have persisted for several decades. Here, by comparing the stomatal opening of guard cells with (crl-ch) or without chloroplasts (crl-no ch) in one epidermis of crl (crumpled leaf) mutant in Arabidopsis, we showed that stomatal apertures of crl-no ch were approximately 65-70% those of crl-ch and approximately 50-60% those of wild type. The weakened stomatal opening in crl-no ch could be partially restored by imposing lower extracellular pH. Correspondingly, the external pH changes and K+ accumulations following fusicoccin (FC) treatment were greatly reduced in the guard cells of crl-no ch compared with crl-ch and wild type. Determination of the relative ATP levels in individual cells showed that crl-no ch guard cells contained considerably lower levels of ATP than did crl-ch and wild type after 2h of white light illumination. In addition, guard cell ATP levels were lower in the epidermis than in leaves, which is consistent with the observed weaker stomatal opening response to white light in the epidermis than in leaves. These results provide evidence that both guard cell chloroplasts and mesophyll contribute to the ATP source for H+ extrusion by guard cells.
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