Journal
PLANT CELL AND ENVIRONMENT
Volume 36, Issue 6, Pages 1093-1104Publisher
WILEY
DOI: 10.1111/pce.12041
Keywords
biofortification; cell wall; P1B-ATPase; phytoremediation; zinc; Zinpyr-1
Categories
Funding
- EU (PHIME) [FOOD-CT-2006-016253]
- MNiSW through the Faculty of Biology, Warsaw University [191128]
- COST project [814/N-COST/2010/0]
- [POIG.02.02.00-14-024/08-0]
Ask authors/readers for more resources
AtHMA4 was previously shown to contribute to the control of Zn root-to-shoot translocation and tolerance to high Zn. However, heterologous expression of 35S::AtHMA4 in tobacco (Nicotiana tabacum cv. Xanthi) results in enhanced Zn sensitivity. This study provides a better understanding of the development of this Zn-sensitive phenotype and demonstrates that substantial modifications of Zn homeostasis occur due to AtHMA4 expression. We show that ectopically expressing AtHMA4 in tobacco results in overloading the root and leaf apoplast with Zn. The tissue and cellular distribution of Zn, monitored using Zinpyr-1, was altered in the AtHMA4-expressing plants compared with wild type. Increased loading of the leaf apoplast with Zn in AtHMA4 transformants induced necrosis; this appeared at lower levels of Zn supply in the transgenics compared with wild type. This study suggests that Zn concentration may be sensed in the apoplast of leaves, and if concentrations are above a certain threshold then particular groups of cells accumulate Zn and necrosis is initiated. Therefore, this could be considered as a mechanism for protecting the other parts of the photosynthetically active leaf from Zn toxicity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available